SSGCID: Target Page

BrovA.00197.a

DNA-binding response regulator

CENTER ID:	BrovA.00197.a
ORGANISM:	Brucella ovis ATCC 25840
ASSOCIATED DISEASE:	Ovine epididymitis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrovA.00197.a.B1.GE38056	Full length( BrovA.00197.a )		1	238

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruOvi136990_0484
BV-BRC:	fig\|444178.3.peg.484
RefSeq:	YP_001257449.1
UniProt:	A0A0H3AWE4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKEGAHILIV DDDKDIRDLL HEFLKRRGMH VSIACNGDEM LDVLSRTPID LVILDVMLPG KSGIEICQDV RRTSRVPIIM LTAIADAADK ILGLEIGADD YIAKPFDPRE LLARIRAVLR RFEGNRSPQR ALTQIYRFAG GTLDCARRRL TSPHDVRVEL TTAEFNLLEA FVKSSQHILS RDQLMEMAGH QAVYGYDRSV DILISRLRKK LEDDPCAPKL ILTIRGGGYQ FGHEVEIA

NT Sequence

TTGAAAGAAG GCGCCCACAT TCTGATCGTC GATGACGACA AGGATATACG CGACCTTCTG CACGAATTTC TCAAACGGCG CGGAATGCAC GTTTCCATCG CCTGCAATGG TGATGAAATG CTGGATGTGC TGAGCCGCAC GCCCATTGAT CTCGTCATTC TGGATGTCAT GCTGCCGGGA AAAAGCGGCA TAGAAATCTG TCAGGATGTG CGGCGCACCT CCCGTGTGCC TATCATTATG CTGACGGCCA TTGCCGATGC AGCCGACAAG ATACTGGGAT TGGAAATCGG CGCGGACGAC TATATCGCCA AGCCCTTCGA CCCGCGCGAG TTGCTTGCGC GCATCCGCGC CGTGCTTCGC CGTTTCGAGG GCAATCGCAG CCCGCAGCGC GCCCTTACGC AGATTTACCG CTTCGCGGGC GGGACGCTCG ACTGCGCCCG GCGGCGCCTC ACATCGCCCC ACGATGTGCG GGTGGAACTG ACGACAGCCG AATTCAACCT GCTCGAAGCT TTCGTCAAAA GCTCGCAGCA TATTCTAAGC CGCGACCAGT TGATGGAAAT GGCAGGCCAT CAGGCCGTCT ATGGCTATGA TCGCAGCGTG GATATTCTCA TCAGCCGCCT GCGCAAGAAG CTGGAGGACG ATCCGTGCGC GCCGAAGCTG ATCCTTACCA TTCGCGGCGG CGGCTACCAG TTCGGGCATG AAGTGGAAAT AGCA

Details for BrovA.00197.a.B1.GE38056

HARVESTED ON:	5/13/2014
SEQUENCED ON:	5/6/2014
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML KEGAHILIVD DDKDIRDLLH EFLKRRGMHV SIACNGDEML DVLSRTPIDL VILDVMLPGK SGIEICQDVR RTSRVPIIML TAIADAADKI LGLEIGADDY IAKPFDPREL LARIRAVLRR FEGNRSPQRA LTQIYRFAGW TLDCARRRLT SPHDVRVELT TAEFNLLEAF VKSSQHILSR DQLMEMAGHQ AVYGYDRSVD ILISRLRKKL EDDPCAPKLI LTIRGGGYQF GHEVEIA

Validated NT Sequence

atcttactca cttatgctat ttccacttca tgcccgaact ggtagccgcc gccgcgaatg gtaaggatca gcttcggcgc gcacggatcg tcctccagct tcttgcgcag gcggctgatg agaatatcca cgctgcgatc atagccatag acggcctgat ggcctgccat ttccatcaac tggtcgcggc ttagaatatg ctgcgagctt ttgacgaaag cttcgagcag gttgaattcg gctgtcgtca gttccacccg cacatcgtgg ggcgatgtaa ggcgccgccg ggcgcagtcg agcgtccagc ccgcgaagcg gtaaatctgc gtaagggcgc gctgcgggct gcgattgccc tcgaaacggc gaagcacggc gcggatgcgc gcaagcaact cgcgcgggtc gaagggcttg gcgatatagt cgtccgcgcc gatttccaat cccagtatct tgtcggctgc atcggcaatg gccgtcagca taatgatagg cacacgggag gtgcgccgca catcctgaca gatttctatg ccgctttttc ccggcagcat gacatccaga atgacgagat caatgggcgt gcggctcagc acatccagca tttcatcacc attgcaggcg atggaaacgt gcattccgcg ccgtttgaga aattcgtgca gaaggtcgcg tatatccttg tcgtcatcga cgatcagaat gtgggcgcct tctttcaaca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

MAHHHHHHMK EGAHILIVDD DKDIRDLLHE FLKRRGMHVS IACNGDEMLD VLSRTPIDLV ILDVMLPGKS GIEICQDVRR TSRVPIIMLT AIADAADKIL GLEIGADDYI AKPFDPRELL ARIRAVLRRF EGNRSPQRAL TQIYRFAGGT LDCARRRLTS PHDVRVELTT AEFNLLEAFV KSSQHILSRD QLMEMAGHQA VYGYDRSVDI LISRLRKKLE DDPCAPKLIL TIRGGGYQFG HEVEIA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgaaag aaggcgccca cattctgatc gtcgatgacg acaaggatat acgcgacctt ctgcacgaat ttctcaaacg gcgcggaatg cacgtttcca tcgcctgcaa tggtgatgaa atgctggatg tgctgagccg cacgcccatt gatctcgtca ttctggatgt catgctgccg ggaaaaagcg gcatagaaat ctgtcaggat gtgcggcgca cctcccgtgt gcctatcatt atgctgacgg ccattgccga tgcagccgac aagatactgg gattggaaat cggcgcggac gactatatcg ccaagccctt cgacccgcgc gagttgcttg cgcgcatccg cgccgtgctt cgccgtttcg agggcaatcg cagcccgcag cgcgccctta cgcagattta ccgcttcgcg ggcgggacgc tcgactgcgc ccggcggcgc ctcacatcgc cccacgatgt gcgggtggaa ctgacgacag ccgaattcaa cctgctcgaa gctttcgtca aaagctcgca gcatattcta agccgcgacc agttgatgga aatggcaggc catcaggccg tctatggcta tgatcgcagc gtggatattc tcatcagccg cctgcgcaag aagctggagg acgatccgtg cgcgccgaag ctgatcctta ccattcgcgg cggcggctac cagttcgggc atgaagtgga aatagcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at