BrovA.00085.c
UDP-glucose 4-epimerase (EC 5.1.3.2)
| CENTER ID: | BrovA.00085.c |
| ORGANISM: | Brucella ovis ATCC 25840 |
| ASSOCIATED DISEASE: | Ovine epididymitis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrovA.00085.c.B1.GE38048 | Full length( BrovA.00085.c ) | 1 | 328 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrovA.00085.c.B1.PS02133 | Full length( BrovA.00085.c ) | 1 | 328 |
Structures
4WOK
DEPOSITED: 10/15/2014
DETERMINATION: XRay
CLONE: BrovA.00085.c.B1.GE38048
PROTEIN: BrovA.00085.c.B1.PS02133
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | PATRIC_Locus_Tag:VBIBruOvi136990_0551 |
| BV-BRC: | fig|444178.3.peg.551 |
| RefSeq: | YP_001257509.1 |
| UniProt: | A0A0H3ASR6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSANNVLVVG GAGFIGSHTA KLLAGQGYAP VVYDNLSTGH QSAVRWGDFV EGDILDQARL VETMEKYVPV AVIHFAASAY VGESVEDPAK YYRNNVGGTQ SLLDACRLTR TQNVIFSSSC ATYGVPSRLP IGEGEAQNPI NPYGRTKLIA EHMLADYAVA YGLRYVALRY FNASGADIDG ELGEKHDPET HLIPRAMMAA AGRLDVLEVY GDDYETPDGT CIRDYIHVTD LARAHVLAVE HLKEAGGNLA VNLGTGRGTS IREIVQSIGR LTGRSVPVAM RARRAGDPPA LYADPALAAE KLGFHTVYSD LDTIIRTAAP YFGLEVRG
NT Sequence
ATGTCCGCGA ACAACGTCCT CGTCGTCGGT GGCGCCGGAT TCATCGGCAG CCACACGGCC AAGCTTCTGG CCGGGCAGGG CTATGCACCC GTCGTCTACG ACAATCTTTC GACGGGCCAT CAATCCGCCG TCCGGTGGGG CGACTTTGTC GAGGGCGACA TTCTTGACCA GGCGCGTCTG GTCGAGACAA TGGAAAAATA TGTGCCGGTC GCGGTAATCC ATTTCGCCGC CTCGGCCTAT GTCGGGGAAT CGGTGGAGGA CCCCGCCAAA TATTATCGCA ACAATGTAGG CGGAACGCAG TCGCTTCTCG ACGCCTGCCG TCTGACGCGC ACGCAAAACG TTATCTTCTC CTCCAGTTGC GCCACCTATG GCGTGCCCAG CCGGCTTCCC ATCGGGGAGG GCGAGGCCCA GAATCCGATC AACCCTTATG GGCGCACAAA GTTGATCGCC GAGCACATGC TGGCCGATTA TGCGGTGGCC TACGGGCTGC GTTATGTGGC GCTACGCTAT TTCAATGCAT CCGGTGCCGA TATCGACGGT GAACTGGGCG AAAAGCACGA CCCTGAAACA CATCTCATCC CGCGCGCCAT GATGGCCGCC GCTGGCAGGC TCGACGTTCT GGAAGTCTAT GGCGACGATT ACGAGACGCC GGACGGCACC TGCATCCGCG ACTATATCCA TGTCACCGAT CTCGCGCGGG CGCATGTGCT GGCGGTCGAG CATCTGAAGG AAGCCGGGGG CAATCTGGCC GTTAATCTGG GTACCGGGCG TGGCACGTCA ATTCGTGAGA TCGTGCAGTC CATTGGCCGC CTGACGGGCC GTAGCGTGCC GGTCGCCATG CGCGCGCGCC GTGCCGGCGA TCCGCCAGCG CTCTATGCCG ACCCGGCACT TGCTGCCGAA AAGCTCGGCT TCCATACGGT CTATTCCGAT CTGGACACTA TCATCCGCAC CGCTGCCCCA TACTTCGGAC TGGAGGTTAG GGGA
Details for BrovA.00085.c.B1.GE38048
| HARVESTED ON: | 5/13/2014 |
| SEQUENCED ON: | 5/6/2014 |
| EXPECTED MW: | 36kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS ANNVLVVGGA GFIGSHTAKL LAGQGYAPVV YDNLSTGHQS AVRWGDFVEG DILDQARLVE TMEKYAPVAV IHFAASAYVG ESVEDPAKYY RNNVGGTQSL LDACRLTRTQ NVIFSSSCAT YGVPSRLPIG EGEAQNPINP YGRTKLIAEH MLADYAVAYG LRYVALRYFN ASGADIDGEL GEKHDPETHL IPRAMMAAAG RLDVLEVYGD DYETPDGTCI RDYIHVTDLA RAHVLAVEHL KEAGGNLAVN LGTGRGTSIR EIVQSIGRLT GRSVPVAMRA RRAGDPPALY ADPALAAEKL GFHTVYSDLD TIIRTAAPHF GLEVRG
Validated NT Sequence
ctttaagaag gagatatacc atggctcacc accaccacca ccatatgtcc gcgaacaacg tcctcgtcgt cggtggcgcc ggattcatcg gcagccacac ggccaagctt ctggccgggc agggctatgc acccgtcgtc tacgacaatc tttcgacggg ccatcaatcc gccgtccggt ggggcgactt tgtcgagggc gacattcttg accaggcgcg tctggtcgag acaatggaaa aatatgcgcc ggtcgcggta atccatttcg ccgcctcggc ctatgtcggg gaatcggtgg aggaccccgc caaatattat cgcaacaatg taggcggaac gcagtcgctt ctcgacgcct gccgtctgac gcgcacgcaa aacgttatct tctcctccag ttgcgccacc tatggcgtgc ccagccggct tcccatcggg gagggcgagg cccagaatcc gatcaaccct tatgggcgca caaagttgat cgccgagcac atgctggccg attatgcggt ggcctacggg ctgcgttatg tggcgctacg ctatttcaat gcatccggtg ccgatatcga cggtgaactg ggcgaaaagc acgaccctga aacacatctc atcccgcgcg ccatgatggc cgccgctggc aggctcgacg ttctggaagt ctatggcgac gattacgaga cgccggacgg cacctgcatc cgcgactata tccatgtcac cgatctcgcg cgggcgcatg tgctggcggt cgagcatctg aaggaagccg ggggcaatct ggccgttaat ctgggtaccg ggcgtggcac gtcaattcgt gagatcgtgc agtccattgg ccgcctgacg ggccgtagcg tgccggtcgc catgcgcgcg cgccgtgccg gcgatccgcc agcgctctat gccgacccgg cacttgctgc cgaaaagctc ggcttccata cggtctattc cgatctggac actatcatcc gcaccgctgc cccacacttc ggactggagg ttaggggata agtgagtaag ataggatccg gctgctaaca a
Expected Protein Sequence
MAHHHHHHMS ANNVLVVGGA GFIGSHTAKL LAGQGYAPVV YDNLSTGHQS AVRWGDFVEG DILDQARLVE TMEKYVPVAV IHFAASAYVG ESVEDPAKYY RNNVGGTQSL LDACRLTRTQ NVIFSSSCAT YGVPSRLPIG EGEAQNPINP YGRTKLIAEH MLADYAVAYG LRYVALRYFN ASGADIDGEL GEKHDPETHL IPRAMMAAAG RLDVLEVYGD DYETPDGTCI RDYIHVTDLA RAHVLAVEHL KEAGGNLAVN LGTGRGTSIR EIVQSIGRLT GRSVPVAMRA RRAGDPPALY ADPALAAEKL GFHTVYSDLD TIIRTAAPYF GLEVRG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg cgaacaacgt cctcgtcgtc ggtggcgccg gattcatcgg cagccacacg gccaagcttc tggccgggca gggctatgca cccgtcgtct acgacaatct ttcgacgggc catcaatccg ccgtccggtg gggcgacttt gtcgagggcg acattcttga ccaggcgcgt ctggtcgaga caatggaaaa atatgtgccg gtcgcggtaa tccatttcgc cgcctcggcc tatgtcgggg aatcggtgga ggaccccgcc aaatattatc gcaacaatgt aggcggaacg cagtcgcttc tcgacgcctg ccgtctgacg cgcacgcaaa acgttatctt ctcctccagt tgcgccacct atggcgtgcc cagccggctt cccatcgggg agggcgaggc ccagaatccg atcaaccctt atgggcgcac aaagttgatc gccgagcaca tgctggccga ttatgcggtg gcctacgggc tgcgttatgt ggcgctacgc tatttcaatg catccggtgc cgatatcgac ggtgaactgg gcgaaaagca cgaccctgaa acacatctca tcccgcgcgc catgatggcc gccgctggca ggctcgacgt tctggaagtc tatggcgacg attacgagac gccggacggc acctgcatcc gcgactatat ccatgtcacc gatctcgcgc gggcgcatgt gctggcggtc gagcatctga aggaagccgg gggcaatctg gccgttaatc tgggtaccgg gcgtggcacg tcaattcgtg agatcgtgca gtccattggc cgcctgacgg gccgtagcgt gccggtcgcc atgcgcgcgc gccgtgccgg cgatccgcca gcgctctatg ccgacccggc acttgctgcc gaaaagctcg gcttccatac ggtctattcc gatctggaca ctatcatccg caccgctgcc ccatacttcg gactggaggt taggggatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BrovA.00085.c.B1.PS02133
| PURIFICATION DATe: | 8/13/2014 |
| CONCENTRATION: | 17mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS ANNVLVVGGA GFIGSHTAKL LAGQGYAPVV YDNLSTGHQS AVRWGDFVEG DILDQARLVE TMEKYAPVAV IHFAASAYVG ESVEDPAKYY RNNVGGTQSL LDACRLTRTQ NVIFSSSCAT YGVPSRLPIG EGEAQNPINP YGRTKLIAEH MLADYAVAYG LRYVALRYFN ASGADIDGEL GEKHDPETHL IPRAMMAAAG RLDVLEVYGD DYETPDGTCI RDYIHVTDLA RAHVLAVEHL KEAGGNLAVN LGTGRGTSIR EIVQSIGRLT GRSVPVAMRA RRAGDPPALY ADPALAAEKL GFHTVYSDLD TIIRTAAPHF GLEVRG
Validated NT Sequence
ctttaagaag gagatatacc atggctcacc accaccacca ccatatgtcc gcgaacaacg tcctcgtcgt cggtggcgcc ggattcatcg gcagccacac ggccaagctt ctggccgggc agggctatgc acccgtcgtc tacgacaatc tttcgacggg ccatcaatcc gccgtccggt ggggcgactt tgtcgagggc gacattcttg accaggcgcg tctggtcgag acaatggaaa aatatgcgcc ggtcgcggta atccatttcg ccgcctcggc ctatgtcggg gaatcggtgg aggaccccgc caaatattat cgcaacaatg taggcggaac gcagtcgctt ctcgacgcct gccgtctgac gcgcacgcaa aacgttatct tctcctccag ttgcgccacc tatggcgtgc ccagccggct tcccatcggg gagggcgagg cccagaatcc gatcaaccct tatgggcgca caaagttgat cgccgagcac atgctggccg attatgcggt ggcctacggg ctgcgttatg tggcgctacg ctatttcaat gcatccggtg ccgatatcga cggtgaactg ggcgaaaagc acgaccctga aacacatctc atcccgcgcg ccatgatggc cgccgctggc aggctcgacg ttctggaagt ctatggcgac gattacgaga cgccggacgg cacctgcatc cgcgactata tccatgtcac cgatctcgcg cgggcgcatg tgctggcggt cgagcatctg aaggaagccg ggggcaatct ggccgttaat ctgggtaccg ggcgtggcac gtcaattcgt gagatcgtgc agtccattgg ccgcctgacg ggccgtagcg tgccggtcgc catgcgcgcg cgccgtgccg gcgatccgcc agcgctctat gccgacccgg cacttgctgc cgaaaagctc ggcttccata cggtctattc cgatctggac actatcatcc gcaccgctgc cccacacttc ggactggagg ttaggggata agtgagtaag ataggatccg gctgctaaca a
Expressed Protein Sequence
MAHHHHHHMS ANNVLVVGGA GFIGSHTAKL LAGQGYAPVV YDNLSTGHQS AVRWGDFVEG DILDQARLVE TMEKYVPVAV IHFAASAYVG ESVEDPAKYY RNNVGGTQSL LDACRLTRTQ NVIFSSSCAT YGVPSRLPIG EGEAQNPINP YGRTKLIAEH MLADYAVAYG LRYVALRYFN ASGADIDGEL GEKHDPETHL IPRAMMAAAG RLDVLEVYGD DYETPDGTCI RDYIHVTDLA RAHVLAVEHL KEAGGNLAVN LGTGRGTSIR EIVQSIGRLT GRSVPVAMRA RRAGDPPALY ADPALAAEKL GFHTVYSDLD TIIRTAAPYF GLEVRG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG CGAACAACGT CCTCGTCGTC GGTGGCGCCG GATTCATCGG CAGCCACACG GCCAAGCTTC TGGCCGGGCA GGGCTATGCA CCCGTCGTCT ACGACAATCT TTCGACGGGC CATCAATCCG CCGTCCGGTG GGGCGACTTT GTCGAGGGCG ACATTCTTGA CCAGGCGCGT CTGGTCGAGA CAATGGAAAA ATATGTGCCG GTCGCGGTAA TCCATTTCGC CGCCTCGGCC TATGTCGGGG AATCGGTGGA GGACCCCGCC AAATATTATC GCAACAATGT AGGCGGAACG CAGTCGCTTC TCGACGCCTG CCGTCTGACG CGCACGCAAA ACGTTATCTT CTCCTCCAGT TGCGCCACCT ATGGCGTGCC CAGCCGGCTT CCCATCGGGG AGGGCGAGGC CCAGAATCCG ATCAACCCTT ATGGGCGCAC AAAGTTGATC GCCGAGCACA TGCTGGCCGA TTATGCGGTG GCCTACGGGC TGCGTTATGT GGCGCTACGC TATTTCAATG CATCCGGTGC CGATATCGAC GGTGAACTGG GCGAAAAGCA CGACCCTGAA ACACATCTCA TCCCGCGCGC CATGATGGCC GCCGCTGGCA GGCTCGACGT TCTGGAAGTC TATGGCGACG ATTACGAGAC GCCGGACGGC ACCTGCATCC GCGACTATAT CCATGTCACC GATCTCGCGC GGGCGCATGT GCTGGCGGTC GAGCATCTGA AGGAAGCCGG GGGCAATCTG GCCGTTAATC TGGGTACCGG GCGTGGCACG TCAATTCGTG AGATCGTGCA GTCCATTGGC CGCCTGACGG GCCGTAGCGT GCCGGTCGCC ATGCGCGCGC GCCGTGCCGG CGATCCGCCA GCGCTCTATG CCGACCCGGC ACTTGCTGCC GAAAAGCTCG GCTTCCATAC GGTCTATTCC GATCTGGACA CTATCATCCG CACCGCTGCC CCATACTTCG GACTGGAGGT TAGGGGATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT