SSGCID: Target Page

BrovA.00085.b

GDP-L-fucose synthetase (EC 1.1.1.271)

CENTER ID:	BrovA.00085.b
ORGANISM:	Brucella ovis ATCC 25840
ASSOCIATED DISEASE:	Ovine epididymitis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrovA.00085.b.B1.GE38047	Full length( BrovA.00085.b )		1	356

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruOvi136990_0424
BV-BRC:	fig\|444178.3.peg.424
RefSeq:	YP_001257406.1
UniProt:	A0A0H3AW26

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQDKTALFVG VTGQDGAYLS ELLLGKGYRV HGLKRRSSSF NMARIDHLYQ DPHEEDIRFR LHFGDLTDAT NLCRVIQEVQ PDEIYNLGAQ SHVQVSFETP EYTANADALG TLRLLESMRI LGLGKSCRFY QASTSELFGN SSHHAQNEQT PFAPRSPYAT AKLYAYWTTV NYRDAYGFHA SNGILFNHES PLRGETFVTR KITRAVAAIE RGLQDRLRLG NLEARRDWGH ARDYVEGMWR ILQEDTPDDY VLATGETHTV REFIEHAFKA VDKQIVWHGD GVDEVGIDRK TGNCLIEIDP RYFRPNEVDF LMGDASKAKD RLGWQHTTGF ENLVAEMVDW DLRNIMREVG RNDFYG

NT Sequence

TTGCAGGATA AAACAGCTCT CTTCGTCGGT GTAACCGGGC AGGATGGCGC CTATCTAAGC GAGCTTCTGC TGGGCAAGGG CTATCGGGTG CATGGCCTGA AAAGGCGGTC ATCGTCATTC AATATGGCGC GTATCGACCA TCTTTATCAA GACCCGCATG AGGAAGACAT CCGCTTTCGC CTGCATTTCG GCGACCTGAC AGACGCAACC AATCTGTGCC GCGTCATCCA GGAAGTGCAG CCTGACGAGA TTTATAATCT CGGCGCCCAG AGCCATGTGC AGGTGAGTTT TGAAACGCCG GAATATACGG CCAATGCCGA TGCGCTCGGC ACACTGCGCC TGCTTGAATC CATGCGCATC CTGGGTCTCG GCAAAAGCTG CCGTTTCTAT CAGGCTTCCA CTTCGGAGCT TTTCGGCAAT AGTTCCCACC ATGCGCAAAA CGAGCAGACG CCTTTCGCTC CGCGCAGCCC ATATGCAACC GCCAAGCTTT ATGCTTACTG GACAACGGTG AACTATCGCG ATGCCTATGG TTTCCACGCC TCGAACGGCA TCTTGTTCAA TCATGAAAGC CCCTTGCGGG GCGAAACCTT CGTCACCCGC AAGATCACGC GCGCAGTTGC AGCCATCGAA CGCGGGCTAC AGGACAGGCT GCGGCTTGGC AATCTTGAGG CCCGCCGCGA CTGGGGCCAT GCGCGCGATT ATGTCGAAGG CATGTGGCGC ATTCTACAGG AAGACACACC GGACGATTAT GTGCTCGCCA CGGGTGAAAC GCATACGGTG CGTGAATTCA TCGAACACGC CTTCAAGGCC GTTGACAAGC AGATCGTATG GCATGGCGAT GGCGTGGACG AGGTCGGCAT CGACCGCAAG ACGGGCAATT GCCTGATCGA GATCGACCCG CGCTATTTCC GCCCCAACGA AGTGGATTTC CTTATGGGCG ATGCTTCCAA GGCAAAAGAC CGTCTGGGCT GGCAACATAC GACCGGCTTT GAAAATCTCG TGGCTGAAAT GGTGGATTGG GATCTGCGCA ACATCATGCG GGAGGTAGGA CGCAATGATT TCTACGGC

Details for BrovA.00085.b.B1.GE38047

HARVESTED ON:	5/13/2014
SEQUENCED ON:	5/6/2014
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMQ DKTALFVGVT GQDGAYLSEL LLGKGYRVHG LKRRSSSFNM ARIDHLYQDP HEEDIRFRLH FGDLTDATNL CRVIQEVQPD EIYNLGAQSH VQVSFETPEY TANADALGTL RLLESMRILG LGKSCRFYQA STSELFGNSS HHAQNEQTPF APRSPYATAK LYAYWTTVNY RDAYGFHASN GILFNHESPL RGETFVTRKI TRAVAAIERG LQDRLRLGNL EARRDWGHAR DYVEGMWRIL QEDTPDDYVL ATGETHTVRE FIEHAFKAVD KQIVWHGDGV DEVGIDRKTG NCLIEIDPRY FRPNEVDFLM GDASKAKDRL GWQHTTGFEN LVAEMVDWDL RNIMREVGRN DFYG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcagg ataaaacagc tctcttcgtc ggtgtaaccg ggcaggatgg cgcctatcta agcgagcttc tgctgggcaa gggctatcgg gtgcatggcc tgaaaaggcg gtcatcgtca ttcaatatgg cgcgtatcga ccatctttat caagacccgc atgaggaaga catccgcttt cgcctgcatt tcggcgacct gacagacgca accaatctgt gccgcgtcat ccaggaagtg cagcctgacg agatttataa tctcggcgcc cagagccatg tgcaggtgag ttttgaaacg ccggaatata cggccaatgc cgatgcgctc ggcacactgc gcctgcttga atccatgcgc atcctgggtc tcggcaaaag ctgccgtttc tatcaggctt ccacttcgga gcttttcggc aatagttccc accatgcgca aaacgagcag acgcctttcg ctccgcgcag cccatatgca accgccaagc tttatgctta ctggacaacg gtgaactatc gcgatgccta tggtttccac gcctcgaacg gcatcttgtt caatcatgaa agccccttgc ggggcgaaac cttcgtcacc cgcaagatca cgcgcgcagt tgcagccatc gaacgcgggc tacaggacag gctgcggctt ggcaatcttg aggcccgccg cgactggggc catgcgcgcg attatgtcga aggcatgtgg cgcattctac aggaagacac accggacgat tatgtgctcg ccacgggtga aacgcatacg gtgcgtgaat tcatcgaaca cgccttcaag gccgttgaca agcagatcgt atggcatggc gatggcgtgg acgaggtcgg catcgaccgc aagacgggca attgcctgat cgagatcgac ccgcgctatt tccgccccaa cgaagtggat ttccttatgg gcgatgcttc caaggcaaaa gaccgtctgg gctggcaaca tacgaccggc tttgaaaatc tcgtggctga aatggtggat tgggatctgc gcaacatcat gcgggaggta ggacgcaatg atttctacgg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat