BrovA.00085.a
fucose synthetase family protein
| CENTER ID: | BrovA.00085.a |
| ORGANISM: | Brucella ovis ATCC 25840 |
| ASSOCIATED DISEASE: | Ovine epididymitis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrovA.00085.a.B1.GE38046 | Full length( BrovA.00085.a ) | 1 | 326 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrovA.00085.a.B1.PS02318 | Full length( BrovA.00085.a ) | 1 | 326 |
Structures
8DU0
DEPOSITED: 7/26/2022
DETERMINATION: XRay
CLONE: BrovA.00085.a.B1.GE38046
PROTEIN: BrovA.00085.a.B1.PS02318
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|444178.3.peg.423 |
| RefSeq: | YP_001257405.1 |
| UniProt: | A0A0H3AU93 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MISTAETGRE PVYSLTGKKI FVAGHTGMVG SAILRRLQHE DCDIITAAHS VLDLTRQGPT ENFISGHRPD VIIIAAARVG GILANSRFPA DFLYDNLAIG MNLIHAAHQI GVERLLWLGS SCIYPRDAAQ PLTEDALLTG PLEPTNEAYA IAKIAGLKYA QSCARQFGDR FITAMPTNLY GPNDNFDPTS SHVLPALIRR VHEARMRGAE EVVLWGSGKP LREFLHVDDL ADACLHLLRF YNGIEPVNIG SGEEISIKEL ALTVARIVGY EGRFEHDLSK PDGTPRKLLD TSRIEALGWQ PRIHLEDGLR DVYRNWLEET AGSVAA
NT Sequence
ATGATTTCTA CGGCTGAGAC CGGGCGCGAA CCCGTCTATT CACTCACGGG AAAGAAAATC TTCGTCGCCG GTCACACCGG CATGGTCGGC TCAGCCATTT TGCGGCGCCT CCAGCATGAG GATTGCGACA TCATCACCGC CGCGCACAGC GTGCTGGACC TGACCCGACA AGGCCCAACG GAAAATTTCA TCAGCGGCCA CAGGCCCGAT GTCATTATCA TCGCTGCCGC GCGCGTTGGC GGCATTCTCG CAAATTCGCG ATTTCCAGCG GATTTTCTCT ATGACAATCT GGCCATCGGC ATGAACCTCA TTCATGCCGC ACATCAGATC GGTGTGGAAC GCCTGCTCTG GCTGGGATCA AGCTGCATCT ATCCGCGCGA CGCCGCCCAG CCACTCACCG AGGATGCCCT GTTGACCGGC CCGCTGGAGC CGACCAACGA GGCCTATGCC ATCGCCAAGA TTGCCGGATT GAAATATGCG CAAAGCTGCG CGCGCCAGTT TGGCGACCGT TTCATCACGG CCATGCCGAC CAATCTTTAT GGACCGAACG ACAATTTCGA TCCCACCAGC TCGCATGTCC TGCCGGCGCT GATAAGGCGG GTGCATGAAG CCAGGATGAG AGGTGCGGAA GAAGTGGTGC TTTGGGGCAG CGGCAAGCCC TTGCGGGAAT TCCTGCATGT GGACGACCTC GCCGATGCGT GCCTGCATCT CCTGCGCTTT TATAATGGCA TCGAGCCGGT GAATATCGGC AGCGGAGAAG AAATCTCGAT CAAGGAACTG GCACTCACCG TTGCACGTAT CGTTGGCTAT GAAGGCCGCT TCGAGCACGA CCTGAGTAAA CCGGACGGCA CGCCGCGCAA GCTGCTCGAT ACATCGCGCA TAGAGGCGCT TGGCTGGCAG CCGCGCATCC ATCTCGAAGA CGGCTTGCGC GATGTCTATC GAAACTGGCT GGAAGAAACC GCAGGCTCCG TCGCCGCT
Details for BrovA.00085.a.B1.GE38046
| HARVESTED ON: | 5/13/2014 |
| SEQUENCED ON: | 5/6/2014 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMI STAETGREPV YSLTGKKIFV AGHTGMVGSA ILRRLQHEDC DIITAAHSVL DLTRQGPTEN FISGHRPDVI IIAAARVGGI LANSRFPADF LYDNLAIGMN LIHAAHQIGV ERLLWLGSSC IYPRDAAQPL TEDALLTGPL EPTNEAYAIA KIAGLKYAQS CARQFGDRFI TAMPTNLYGP NDNFDPTSSH VLPALIRRVH EARMRGAEEV VLWGSGKPLR EFLHVDDLAD ACLHLLRFYN GIEPVNIGSG EEISIKELAL TVARIVGYEG RFEHDLSKPD GTPRKLLDTS RIEALGWQPR IHLEDGLRDV YRNWLEETAG SVAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattt ctacggctga gaccgggcgc gaacccgtct attcactcac gggaaagaaa atcttcgtcg ccggtcacac cggcatggtc ggctcagcca ttttgcggcg cctccagcat gaggattgcg acatcatcac cgccgcgcac agcgtgctgg acctgacccg acaaggccca acggaaaatt tcatcagcgg ccacaggccc gatgtcatta tcatcgctgc cgcgcgcgtt ggcggcattc tcgcaaattc gcgatttcca gcggattttc tctatgacaa tctggccatc ggcatgaacc tcattcatgc cgcacatcag atcggtgtgg aacgcctgct ctggctggga tcaagctgca tctatccgcg cgacgccgcc cagccactca ccgaggatgc cctgttgacc ggcccgctgg agccgaccaa cgaggcctat gccatcgcca agattgccgg attgaaatat gcgcaaagct gcgcgcgcca gtttggcgac cgtttcatca cggccatgcc gaccaatctt tatggaccga acgacaattt cgatcccacc agctcgcatg tcctgccggc gctgataagg cgggtgcatg aagccaggat gagaggtgcg gaagaagtgg tgctttgggg cagcggcaag cccttgcggg aattcctgca tgtggacgac ctcgccgatg cgtgcctgca tctcctgcgc ttttataatg gcatcgagcc ggtgaatatc ggcagcggag aagaaatctc gatcaaggaa ctggcactca ccgttgcacg tatcgttggc tatgaaggcc gcttcgagca cgacctgagt aaaccggacg gcacgccgcg caagctgctc gatacatcgc gcatagaggc gcttggctgg cagccgcgca tccatctcga agacggcttg cgcgatgtct atcgaaactg gctggaagaa accgcaggct ccgtcgccgc ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BrovA.00085.a.B1.PS02318
| PURIFICATION DATe: | 2/3/2015 |
| CONCENTRATION: | 36.28mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMI STAETGREPV YSLTGKKIFV AGHTGMVGSA ILRRLQHEDC DIITAAHSVL DLTRQGPTEN FISGHRPDVI IIAAARVGGI LANSRFPADF LYDNLAIGMN LIHAAHQIGV ERLLWLGSSC IYPRDAAQPL TEDALLTGPL EPTNEAYAIA KIAGLKYAQS CARQFGDRFI TAMPTNLYGP NDNFDPTSSH VLPALIRRVH EARMRGAEEV VLWGSGKPLR EFLHVDDLAD ACLHLLRFYN GIEPVNIGSG EEISIKELAL TVARIVGYEG RFEHDLSKPD GTPRKLLDTS RIEALGWQPR IHLEDGLRDV YRNWLEETAG SVAA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATTT CTACGGCTGA GACCGGGCGC GAACCCGTCT ATTCACTCAC GGGAAAGAAA ATCTTCGTCG CCGGTCACAC CGGCATGGTC GGCTCAGCCA TTTTGCGGCG CCTCCAGCAT GAGGATTGCG ACATCATCAC CGCCGCGCAC AGCGTGCTGG ACCTGACCCG ACAAGGCCCA ACGGAAAATT TCATCAGCGG CCACAGGCCC GATGTCATTA TCATCGCTGC CGCGCGCGTT GGCGGCATTC TCGCAAATTC GCGATTTCCA GCGGATTTTC TCTATGACAA TCTGGCCATC GGCATGAACC TCATTCATGC CGCACATCAG ATCGGTGTGG AACGCCTGCT CTGGCTGGGA TCAAGCTGCA TCTATCCGCG CGACGCCGCC CAGCCACTCA CCGAGGATGC CCTGTTGACC GGCCCGCTGG AGCCGACCAA CGAGGCCTAT GCCATCGCCA AGATTGCCGG ATTGAAATAT GCGCAAAGCT GCGCGCGCCA GTTTGGCGAC CGTTTCATCA CGGCCATGCC GACCAATCTT TATGGACCGA ACGACAATTT CGATCCCACC AGCTCGCATG TCCTGCCGGC GCTGATAAGG CGGGTGCATG AAGCCAGGAT GAGAGGTGCG GAAGAAGTGG TGCTTTGGGG CAGCGGCAAG CCCTTGCGGG AATTCCTGCA TGTGGACGAC CTCGCCGATG CGTGCCTGCA TCTCCTGCGC TTTTATAATG GCATCGAGCC GGTGAATATC GGCAGCGGAG AAGAAATCTC GATCAAGGAA CTGGCACTCA CCGTTGCACG TATCGTTGGC TATGAAGGCC GCTTCGAGCA CGACCTGAGT AAACCGGACG GCACGCCGCG CAAGCTGCTC GATACATCGC GCATAGAGGC GCTTGGCTGG CAGCCGCGCA TCCATCTCGA AGACGGCTTG CGCGATGTCT ATCGAAACTG GCTGGAAGAA ACCGCAGGCT CCGTCGCCGC TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT