SSGCID: Target Page

BrovA.00027.e

Glutaryl-CoA dehydrogenase (EC 1.3.99.7)

CENTER ID:	BrovA.00027.e
ORGANISM:	Brucella ovis ATCC 25840
ASSOCIATED DISEASE:	Ovine epididymitis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrovA.00027.e.B1.GE38044	Full length( BrovA.00027.e )		1	324

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruOvi136990_2406

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRAAFAWED PFLLEEQLTE DERMIRDSAK AFASDVLLPR VEKAYLEETT DPELFHLMGQ AGLLGVTLPE DYGAANASYV AYGLVAREVE RIDSGYRSMM SVQSSLVMYP IYAYGSDEQR KKYLPGLVSG ELIGCFGLTE PDAGSDPAGM KTRAEKIDGG YRLSGSKMWI SNSPIADVFV VWAKSAAHDN AIRGFILEKG MKGLSAPKIG GKLSLRASIT GEIVMDGVEV SEDAILPNVS GLKGPFGCLN RARYGISWGV LGAAEDCWFR ARQYGLDRKQ FNKPLAGTQL YQKKLADMQT EIALGFRHRF ALAACSMKAR WRPK

NT Sequence

ATGTCACGTG CGGCTTTTGC CTGGGAAGAT CCGTTTTTGC TGGAGGAGCA ACTGACCGAA GACGAGCGCA TGATCCGCGA CTCGGCCAAG GCCTTTGCAA GCGACGTTCT TCTGCCTCGT GTCGAAAAAG CCTATCTGGA AGAAACCACC GACCCGGAAC TGTTTCACCT CATGGGGCAG GCGGGGCTTC TGGGCGTAAC GCTGCCGGAA GACTATGGCG CGGCCAATGC AAGCTATGTA GCCTATGGGC TGGTGGCGCG CGAAGTGGAG CGCATTGATT CCGGATATCG TTCCATGATG AGCGTGCAAT CGTCGCTCGT CATGTATCCG ATCTATGCCT ATGGTTCGGA TGAACAGCGC AAGAAATATC TTCCCGGCCT GGTTTCGGGT GAATTGATTG GCTGTTTCGG CCTTACCGAA CCCGATGCGG GTTCCGACCC GGCTGGCATG AAAACCCGCG CCGAAAAGAT CGATGGCGGC TATCGCCTCA GCGGCTCGAA GATGTGGATT TCCAATTCAC CGATTGCCGA TGTGTTCGTC GTCTGGGCAA AATCGGCTGC GCATGACAAC GCCATTCGCG GCTTCATTCT TGAAAAGGGC ATGAAGGGAC TTTCAGCACC GAAGATCGGG GGCAAGCTTT CGCTGCGCGC TTCGATCACC GGCGAGATCG TGATGGATGG GGTTGAAGTT TCCGAAGACG CAATCCTGCC GAATGTTTCC GGTCTCAAGG GCCCGTTTGG CTGTCTCAAC CGCGCGCGCT ATGGCATTTC GTGGGGTGTG CTCGGGGCTG CTGAAGATTG CTGGTTCCGT GCGCGCCAAT ATGGTCTGGA CCGCAAGCAA TTCAACAAGC CGCTGGCTGG CACGCAGCTT TACCAGAAGA AGCTTGCCGA TATGCAGACG GAAATCGCGC TTGGCTTCAG GCATCGCTTC GCGTTGGCCG CCTGTTCGAT GAAGGCAAGA TGGCGCCCGA AA

Details for BrovA.00027.e.B1.GE38044

HARVESTED ON:	5/13/2014
SEQUENCED ON:	5/6/2014
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	95
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS RAAFAWEDPF LLEEQLTEDE RMIRDSAKAF ASDVLLPRVE KAYLEETTDP ELFHLMGQAG LLGVTLPEDY GAANASYVAY GLVAREVERI DSGYRSMMSV QSSLVMYPIY AYGSDEQRKK YLPGLVSGEL IGCFGLTEPD AGSDPAGMKT RAEKIDGGYR LSGSKMWISN SPIADVFVVW AKSAAHDNAI RGFILEKGMK GLSAPKIGGK LSLRASITGE IVMDGVEVSE DAILPNVSGL KGPFGCLNRA RYGISWGVLG AAEDCWFRAR QYGLDRKQFN KPLAGTQLYQ KKLADMQTEI ALGLQASLRV GPLFDEXRWR PK

Validated NT Sequence

cnnnnnnnnn annnntnttt ntttaacttt aagaaggaga tataccatgg ctcaccacca ccaccaccat atgtcacgtg cggcttttgc ctgggaagat ccgtttttgc tggaggagca actgaccgaa gacgagcgca tgatccgcga ctcggccaag gcctttgcaa gcgacgttct tctgcctcgt gtcgaaaaag cctatctgga agaaaccacc gacccggaac tgtttcacct catggggcag gcggggcttc tgggcgtaac gctgccggaa gactatggcg cggccaatgc aagctatgta gcctatgggc tggtggcgcg cgaagtggag cgcattgatt ccggatatcg ttccatgatg agcgtgcaat cgtcgctcgt catgtatccg atctatgcct atggttcgga tgaacagcgc aagaaatatc ttcccggcct ggtttcgggt gaattgattg gctgtttcgg ccttaccgaa cccgatgcgg gttccgaccc ggctggcatg aaaacccgcg ccgaaaagat cgatggcggc tatcgcctca gcggctcgaa gatgtggatt tccaattcac cgattgccga tgtgttcgtc gtctgggcaa aatcggctgc gcatgacaac gccattcgcg gcttcattct tgaaaagggc atgaagggac tttcagcacc gaagatcggg ggcaagcttt cgctgcgcgc ttcgatcacc ggcgagatcg tgatggatgg ggttgaagtt tccgaagacg caatcctgcc gaatgtttcc ggtctcaagg gcccgtttgg ctgtctcaac cgcgcgcgct atggcatttc gtggggtgtg ctcggggctg ctgaagattg ctggttccgt gcgcgccaat atggtctgga ccgcaagcaa ttcaacaagc cgctggctgg cacgcagctt taccagaaga agcttgccga tatgcagacg gaaatcgcgc ttgggcttca ggcatcgctt cgcgttgggc cnctgttcga tgaancaaga tggcgcccga aataagtgag taagatagga tnnntgcnnn aaanncccna ngagnng

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcac gtgcggcttt tgcctgggaa gatccgtttt tgctggagga gcaactgacc gaagacgagc gcatgatccg cgactcggcc aaggcctttg caagcgacgt tcttctgcct cgtgtcgaaa aagcctatct ggaagaaacc accgacccgg aactgtttca cctcatgggg caggcggggc ttctgggcgt aacgctgccg gaagactatg gcgcggccaa tgcaagctat gtagcctatg ggctggtggc gcgcgaagtg gagcgcattg attccggata tcgttccatg atgagcgtgc aatcgtcgct cgtcatgtat ccgatctatg cctatggttc ggatgaacag cgcaagaaat atcttcccgg cctggtttcg ggtgaattga ttggctgttt cggccttacc gaacccgatg cgggttccga cccggctggc atgaaaaccc gcgccgaaaa gatcgatggc ggctatcgcc tcagcggctc gaagatgtgg atttccaatt caccgattgc cgatgtgttc gtcgtctggg caaaatcggc tgcgcatgac aacgccattc gcggcttcat tcttgaaaag ggcatgaagg gactttcagc accgaagatc gggggcaagc tttcgctgcg cgcttcgatc accggcgaga tcgtgatgga tggggttgaa gtttccgaag acgcaatcct gccgaatgtt tccggtctca agggcccgtt tggctgtctc aaccgcgcgc gctatggcat ttcgtggggt gtgctcgggg ctgctgaaga ttgctggttc cgtgcgcgcc aatatggtct ggaccgcaag caattcaaca agccgctggc tggcacgcag ctttaccaga agaagcttgc cgatatgcag acggaaatcg cgcttggctt caggcatcgc ttcgcgttgg ccgcctgttc gatgaaggca agatggcgcc cgaaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat