BrovA.00027.b

Butyryl-CoA dehydrogenase (EC 1.3.99.2)

CENTER ID:	BrovA.00027.b
ORGANISM:	Brucella ovis ATCC 25840
ASSOCIATED DISEASE:	Ovine epididymitis
CURRENT STATUS:	crystal structure
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrovA.00027.b.B1.GE38041	Full length( BrovA.00027.b )		1	375

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|444178.3.peg.461
RefSeq:	YP_001257429.1
UniProt:	A0A0H3AV31

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLLTDTQEQI REAARDFAQE RLAPGAAARD REHAFPRAEL TEMGALGFLG MLVPEEWGGS DLGMVAYALA LEEIAAGDGA CSIIVSVHSS VGCMPILRFG TEDQKRRFLP KMACGEWIGG FALTEPQAGS DASALKTRAR LDGDHYVIDG SKQFITSGKN GNVVIVFAVT DPAAGKKGIS AFIVPTDTPG YEVMSVEHKL GQHSSDTCAL GFTNMRVPVE NRLGAEGEGY KIALANLEGG RIGIAAQAVG MARAAFEAAR DYARERITFG KPIIEHQAVA FRLADMATRI ETARQMVLHA AALREAGKPC LTEASMAKLV ASEMAEQVCS AAIQIHGGYG YLADYPVERI YRDVRVCQIY EGTSDVQRLV IARGL

NT Sequence

ATGCTTCTGA CCGATACGCA GGAACAGATC CGCGAAGCCG CCCGCGACTT CGCACAGGAG CGCCTTGCCC CCGGCGCCGC GGCGCGTGAC CGTGAACATG CATTTCCCCG CGCCGAACTG ACTGAAATGG GCGCGCTTGG CTTTCTCGGA ATGCTGGTGC CGGAAGAATG GGGCGGCTCC GACCTCGGCA TGGTTGCCTA TGCTCTGGCG CTGGAAGAGA TAGCGGCAGG CGACGGTGCC TGCTCGATCA TCGTATCGGT GCATTCCTCC GTCGGCTGTA TGCCGATCTT ACGTTTCGGC ACGGAAGACC AGAAGCGCCG CTTCCTGCCC AAAATGGCCT GTGGCGAATG GATCGGCGGC TTTGCCCTCA CCGAGCCGCA GGCCGGTTCC GATGCCTCCG CGCTGAAAAC GCGCGCCCGG CTCGACGGCG ATCATTATGT GATCGACGGT TCCAAGCAGT TCATCACATC AGGCAAGAAC GGCAATGTCG TGATCGTTTT TGCCGTTACC GATCCTGCCG CAGGCAAGAA GGGTATTTCC GCCTTCATCG TGCCCACCGA TACGCCAGGT TATGAAGTGA TGTCGGTCGA ACACAAGCTC GGCCAGCATT CATCCGACAC CTGTGCGCTC GGCTTCACCA ATATGCGCGT TCCGGTCGAA AACCGGCTGG GCGCTGAAGG CGAAGGCTAC AAGATCGCCC TTGCCAATCT GGAAGGCGGA CGCATCGGCA TCGCAGCCCA GGCGGTTGGC ATGGCGCGCG CCGCTTTCGA GGCGGCACGC GATTATGCCC GCGAACGCAT TACCTTCGGC AAGCCGATCA TCGAACATCA GGCCGTGGCC TTCCGCCTTG CCGACATGGC AACCCGGATC GAAACCGCCC GGCAGATGGT GTTACACGCC GCTGCCCTGC GCGAAGCAGG CAAGCCATGC CTTACCGAAG CTTCAATGGC AAAGCTGGTC GCATCGGAAA TGGCCGAACA GGTCTGTTCC GCAGCCATCC AGATTCATGG CGGCTATGGC TATCTGGCCG ATTATCCGGT CGAGCGCATC TATCGCGATG TCCGCGTCTG CCAGATTTAT GAAGGCACAA GCGACGTGCA GCGGCTCGTC ATCGCGCGCG GGCTA

Details for BrovA.00027.b.B1.GE38041

HARVESTED ON:	5/13/2014
SEQUENCED ON:	5/7/2014
EXPECTED MW:	41kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHML LTDTQEQIRE AARDFAQERL APGAAARDRE HAFPRAELTE MGALGFLGML VPEEWGGSDL GMVAYALALE EIAAGDGACS IIVSVHSSVG CMPILRFGTE DQKRRFLPKM ACGEWIGGFA LTEPQAGSDA SALKTRARLD GDHYVIDGSK QFITSGKNGN VVIVFAVTDP AAGKKGISAF IVPTDTPGYE VMSVEHKLGQ HSSDTCALGF TNMRVPVENR LGAEGEGYKI ALANLEGGRI GIAAQAVGMA RAAFEAARDY ARERITFGKP IIEHQAVAFR LADMATRIET ARQMVLHAAA LREAGKPCLT EASMAKLVAS EMAEQVCSAA IQIHGGYGYL ADYPVERIYR DVRVCQIYEG TSDVQRLVIA RGL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcttc tgaccgatac gcaggaacag atccgcgaag ccgcccgcga cttcgcacag gagcgccttg cccccggcgc cgcggcgcgt gaccgtgaac atgcatttcc ccgcgccgaa ctgactgaaa tgggcgcgct tggctttctc ggaatgctgg tgccggaaga atggggcggc tccgacctcg gcatggttgc ctatgctctg gcgctggaag agatagcggc aggcgacggt gcctgctcga tcatcgtatc ggtgcattcc tccgtcggct gtatgccgat cttacgtttc ggcacggaag accagaagcg ccgcttcctg cccaaaatgg cctgtggcga atggatcggc ggctttgccc tcaccgagcc gcaggccggt tccgatgcct ccgcgctgaa aacgcgcgcc cggctcgacg gcgatcatta tgtgatcgac ggttccaagc agttcatcac atcaggcaag aacggcaatg tcgtgatcgt ttttgccgtt accgatcctg ccgcaggcaa gaagggtatt tccgccttca tcgtgcccac cgatacgcca ggttatgaag tgatgtcggt cgaacacaag ctcggccagc attcatccga cacctgtgcg ctcggcttca ccaatatgcg cgttccggtc gaaaaccggc tgggcgctga aggcgaaggc tacaagatcg cccttgccaa tctggaaggc ggacgcatcg gcatcgcagc ccaggcggtt ggcatggcgc gcgccgcttt cgaggcggca cgcgattatg cccgcgaacg cattaccttc ggcaagccga tcatcgaaca tcaggccgtg gccttccgcc ttgccgacat ggcaacccgg atcgaaaccg cccggcagat ggtgttacac gccgctgccc tgcgcgaagc aggcaagcca tgccttaccg aagcttcaat ggcaaagctg gtcgcatcgg aaatggccga acaggtctgt tccgcagcca tccagattca tggcggctat ggctatctgg ccgattatcc ggtcgagcgc atctatcgcg atgtccgcgt ctgccagatt tatgaaggca caagcgacgt gcagcggctc gtcatcgcgc gcgggctatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat