BrovA.00010.a
Oxidoreductase, short chain dehydrogenase/reductase family
| CENTER ID: | BrovA.00010.a |
| ORGANISM: | Brucella ovis ATCC 25840 |
| ASSOCIATED DISEASE: | Ovine epididymitis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrovA.00010.a.B1.GE38036 | Full length( BrovA.00010.a ) | 1 | 250 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5ER6
DEPOSITED: 11/13/2015
DETERMINATION: XRay
CLONE: BrovA.00010.a.B1.GE38036
PROTEIN: BrovA.00010.a.B1.PS02326
5HA5
DEPOSITED: 12/30/2015
DETERMINATION: XRay
CLONE: BrovA.00010.a.B1.GE38036
PROTEIN: BrovA.00010.a.B1.PS02326
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|444178.3.peg.33 |
| RefSeq: | YP_001257113.1 |
| UniProt: | A0A0H3ATY4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MELLKEKLVL VTGAGRGLGA AISSGAAEQG ARVILVDIDG TAAKAQADAL TAKGFVAEGH ALDVTDRDAV AALADDILSR FGGLDVLVNN AGVAGRAAFD QPEAVEVWDR VIGVNLEGAF NVSHALVPAL KAAKGNVVHL CSVAGFVSGG STAGYVVSKG AIRSLTQVMA RDLAPHGIRV NAVAPGIMMS EMAVAQLNRP GGTDWFMNRV MMKRIGETSE VVDPVVFLAS PMASYITGTI LPVDGGFLAA
NT Sequence
ATGGAACTTC TGAAAGAAAA GCTCGTCCTC GTCACCGGTG CAGGCCGGGG GCTGGGAGCG GCGATTTCTT CCGGCGCGGC AGAGCAGGGC GCGCGCGTCA TTCTCGTTGA TATCGACGGC ACGGCAGCCA AGGCCCAGGC CGATGCGCTG ACCGCGAAAG GCTTCGTTGC CGAAGGCCAT GCGCTCGATG TCACCGACCG CGACGCAGTT GCGGCACTGG CTGACGATAT ATTGAGCCGT TTTGGCGGTC TTGACGTATT GGTGAACAAT GCCGGTGTTG CCGGTCGCGC CGCCTTCGAC CAGCCCGAGG CGGTCGAGGT CTGGGACCGG GTGATCGGCG TCAACCTTGA AGGCGCGTTC AATGTTTCCC ATGCGCTCGT GCCTGCGCTG AAGGCTGCGA AGGGCAATGT GGTGCATCTG TGCTCGGTCG CGGGTTTCGT GTCCGGCGGC TCCACGGCGG GTTATGTCGT CTCCAAGGGG GCGATCCGTT CGCTGACGCA GGTGATGGCG CGTGATCTGG CCCCGCATGG CATCCGCGTC AATGCGGTTG CGCCCGGCAT CATGATGAGC GAGATGGCGG TGGCCCAGCT CAACCGTCCG GGTGGCACAG ACTGGTTTAT GAACCGTGTG ATGATGAAGC GCATCGGCGA AACCAGCGAA GTCGTCGATC CCGTCGTTTT TCTCGCGTCC CCGATGGCAA GCTATATCAC CGGCACCATC CTGCCGGTGG ATGGCGGCTT CCTGGCCGCG
Details for BrovA.00010.a.B1.GE38036
| HARVESTED ON: | 5/13/2014 |
| SEQUENCED ON: | 5/6/2014 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHME LLKEKLVLVT GAGRGLGAAI SSGAAEQGAR VILVDIDGTA AKAQADALTA KGFVAEGHAL DVTDRDAVAA LADDILSRFG GLDVLVNNAG VAGRAAFDQP EAVEVWDRVI GVNLEGAFNV SHALVPALKA AKGNVVHLCS VAGFVSGGST AGYVVSKGAI RSLTQVMARD LAPHGIRVNA VAPGIMMSEM AVAQLNRPGG TDWFMNRVMM KRIGETSEVV DPVVFLASPM ASYITGTILP VDGGFLAA
Validated NT Sequence
tnnntgnntt nnnntttnan aaggagatat accatggctc accaccacca ccaccatatg gaacttctga aagaaaagct cgtcctcgtc accggtgcag gccgggggct gggagcggcg atttcttccg gcgcggcaga gcagggcgcg cgcgtcattc tcgttgatat cgacggcacg gcagccaagg cccaggccga tgcgctgacc gcgaaaggct tcgttgccga aggccatgcg ctcgatgtca ccgaccgcga cgcagttgcg gcactggctg acgatatatt gagccgtttt ggcggtcttg acgtattggt gaacaatgcc ggtgttgccg gtcgcgccgc cttcgaccag cccgaggcgg tcgaggtctg ggaccgggtg atcggcgtca accttgaagg cgcgttcaat gtttcccatg cgctcgtgcc tgcgctgaag gccgcgaagg gcaatgtggt gcatctgtgc tcggtcgcgg gtttcgtgtc cggcggctcc acggcgggtt atgtcgtctc caagggggcg atccgttcgc tgacgcaggt gatggcgcgt gatctggccc cgcatggcat ccgcgtcaat gcggttgcgc ccggcatcat gatgagcgag atggcggtgg cccagctcaa ccgtccgggt ggcacagact ggtttatgaa ccgtgtgatg atgaagcgca tcggcgaaac cagcgaagtc gtcgatcccg tcgtttttct cgcgtccccg atggcaagct atatcaccgg caccatcctg ccggtggatg gcggcttcct ggccgcgtaa gtgagtaaga taggatccgg ctgctacana cccccaaagn aa
Expected Protein Sequence
MAHHHHHHME LLKEKLVLVT GAGRGLGAAI SSGAAEQGAR VILVDIDGTA AKAQADALTA KGFVAEGHAL DVTDRDAVAA LADDILSRFG GLDVLVNNAG VAGRAAFDQP EAVEVWDRVI GVNLEGAFNV SHALVPALKA AKGNVVHLCS VAGFVSGGST AGYVVSKGAI RSLTQVMARD LAPHGIRVNA VAPGIMMSEM AVAQLNRPGG TDWFMNRVMM KRIGETSEVV DPVVFLASPM ASYITGTILP VDGGFLAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaac ttctgaaaga aaagctcgtc ctcgtcaccg gtgcaggccg ggggctggga gcggcgattt cttccggcgc ggcagagcag ggcgcgcgcg tcattctcgt tgatatcgac ggcacggcag ccaaggccca ggccgatgcg ctgaccgcga aaggcttcgt tgccgaaggc catgcgctcg atgtcaccga ccgcgacgca gttgcggcac tggctgacga tatattgagc cgttttggcg gtcttgacgt attggtgaac aatgccggtg ttgccggtcg cgccgccttc gaccagcccg aggcggtcga ggtctgggac cgggtgatcg gcgtcaacct tgaaggcgcg ttcaatgttt cccatgcgct cgtgcctgcg ctgaaggctg cgaagggcaa tgtggtgcat ctgtgctcgg tcgcgggttt cgtgtccggc ggctccacgg cgggttatgt cgtctccaag ggggcgatcc gttcgctgac gcaggtgatg gcgcgtgatc tggccccgca tggcatccgc gtcaatgcgg ttgcgcccgg catcatgatg agcgagatgg cggtggccca gctcaaccgt ccgggtggca cagactggtt tatgaaccgt gtgatgatga agcgcatcgg cgaaaccagc gaagtcgtcg atcccgtcgt ttttctcgcg tccccgatgg caagctatat caccggcacc atcctgccgg tggatggcgg cttcctggcc gcgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat