BrmeB.18158.b
Transcriptional regulator, GntR family
| CENTER ID: | BrmeB.18158.b |
| ORGANISM: | Brucella melitensis ATCC 23457 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeB.18158.b.B1.GE37807 | Full length( BrmeB.18158.b ) | 1 | 257 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
7U5Q
DEPOSITED: 3/2/2022
DETERMINATION: XRay
CLONE: BrmeB.18158.b.B1.GE37807
PROTEIN: BrmeB.18158.b.B1.PS02075
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|546272.3.peg.3076 |
| RefSeq: | YP_002734548.1 |
| UniProt: | C0RLW1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRGRSAQPAL EKQKNVADTE SGAATGVKAR TLPSRVAGVQ RVTTAKLIYH ELQQQIIRME LLPGTPLNEK ALTEKYGVSR TPVREALIRL AEDRLVDVFP QSGTFVARIP VDAIPEAVVI RQALEGETAE RAAANSTAAA IEKLDELIHL QTFYARKDKP GPFHETDDAF HETIAEIAGY PGIWQHLKPV KMQIDRARRM TMPILGRMEQ VLREHHAIRD AISARDVHAA REAMKHHLSA VLPDIDELRK SRPDYFA
NT Sequence
ATGAGAGGAC GCAGTGCGCA ACCGGCGCTT GAAAAACAGA AAAATGTGGC GGACACCGAG AGCGGTGCGG CCACAGGCGT AAAGGCGCGC ACCTTGCCTT CGCGGGTGGC CGGGGTACAG CGCGTCACGA CAGCCAAGCT CATCTATCAT GAACTGCAAC AGCAAATTAT CCGGATGGAG CTTCTTCCCG GCACACCGCT CAATGAAAAG GCGTTGACCG AAAAATATGG CGTCAGCCGC ACGCCGGTAC GGGAAGCATT GATCCGCCTT GCCGAAGATC GTCTGGTCGA TGTTTTCCCG CAATCGGGTA CATTCGTGGC GCGCATTCCG GTTGATGCTA TCCCGGAAGC TGTTGTCATA CGACAGGCGC TGGAGGGAGA AACGGCAGAA AGGGCTGCCG CCAATTCAAC CGCAGCCGCT ATCGAGAAGC TGGACGAGTT GATCCACTTG CAGACTTTTT ATGCCCGCAA GGACAAACCC GGTCCATTTC ATGAAACCGA TGACGCTTTC CACGAAACAA TCGCGGAAAT TGCTGGCTAT CCCGGCATTT GGCAGCATCT CAAGCCGGTG AAAATGCAGA TTGACCGCGC CCGCCGCATG ACCATGCCGA TTCTCGGGCG AATGGAGCAG GTGTTGCGTG AACATCATGC CATTCGCGAT GCCATCTCGG CCCGTGATGT CCATGCCGCG CGCGAAGCCA TGAAACATCA TTTAAGTGCT GTTTTGCCGG ATATTGACGA GTTGCGCAAA AGCCGTCCGG ACTATTTTGC T
Details for BrmeB.18158.b.B1.GE37807
| HARVESTED ON: | 3/6/2014 |
| SEQUENCED ON: | 3/13/2014 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMR GRSAQPALEK QKNVADTESG AATGVKARTL PSRVAGVQRV TTAKLIYHEL QQQIIRMELL PGTPLNEKAL TEKYGVSRTP VREALIRLAE DRLVDVFPQS GTFVARIPVD AIPEAVVIRQ ALEGETAERA AANSTAAAIE KLDELIHLQT FYARKDKPGP FHETDDAFHE TIAEIAGYPG IWQHLKPVKM QIDRARRMTM PILGRMEQVL REHHAIRDAI SARDVHAARE AMKHHLSAVL PDIDELRKSR PDYFA
Validated NT Sequence
canccggntc ctatcttact cacttaagca aaatagtccg gacggctttt gcgcaactcg tcaatatccg gcaaaacagc acttaaatga tgtttcatgg cttcgcgcgc ggcatggaca tcacgggccg agatggcatc gcgaatggca tgatgttcac gcaacacctg ctccattcgc ccgagaatcg gcatggtcat gcggcgggcg cggtcaatct gcattttcac cggcttgaga tgctgccaaa tgccgggata gccagcaatt tccgcgattg tttcgtggaa agcgtcatcg gtttcatgaa atggaccggg tttgtccttg cgggcataaa aagtctgcaa gtggatcaac tcgtccagct tctcgatagc ggctgcggtt gaattggcgg cagccctttc tgccgtttct ccctccagcg cctgtcgtat gacaacagct tccgggatag catcaaccgg aatgcgcgcc acgaatgtac ccgattgcgg gaaaacatcg accagacgat cttcggcaag gcggatcaat gcttcccgta ccggcgtgcg gctgacgcca tatttttcgg tcaacgcctt ttcattgagc ggtgtgccgg gaagaagctc catccggata atttgctgtt gcagttcatg atagatgagc ttggctgtcg tgacgcgctg taccccggcc acccgcgaag gcaaggtgcg cgcctttacg cctgtggccg caccgctctc ggtgtccgcc acatttttct gtttttcaag cgccggttgc gcactgcgtc ctctcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMR GRSAQPALEK QKNVADTESG AATGVKARTL PSRVAGVQRV TTAKLIYHEL QQQIIRMELL PGTPLNEKAL TEKYGVSRTP VREALIRLAE DRLVDVFPQS GTFVARIPVD AIPEAVVIRQ ALEGETAERA AANSTAAAIE KLDELIHLQT FYARKDKPGP FHETDDAFHE TIAEIAGYPG IWQHLKPVKM QIDRARRMTM PILGRMEQVL REHHAIRDAI SARDVHAARE AMKHHLSAVL PDIDELRKSR PDYFA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagag gacgcagtgc gcaaccggcg cttgaaaaac agaaaaatgt ggcggacacc gagagcggtg cggccacagg cgtaaaggcg cgcaccttgc cttcgcgggt ggccggggta cagcgcgtca cgacagccaa gctcatctat catgaactgc aacagcaaat tatccggatg gagcttcttc ccggcacacc gctcaatgaa aaggcgttga ccgaaaaata tggcgtcagc cgcacgccgg tacgggaagc attgatccgc cttgccgaag atcgtctggt cgatgttttc ccgcaatcgg gtacattcgt ggcgcgcatt ccggttgatg ctatcccgga agctgttgtc atacgacagg cgctggaggg agaaacggca gaaagggctg ccgccaattc aaccgcagcc gctatcgaga agctggacga gttgatccac ttgcagactt tttatgcccg caaggacaaa cccggtccat ttcatgaaac cgatgacgct ttccacgaaa caatcgcgga aattgctggc tatcccggca tttggcagca tctcaagccg gtgaaaatgc agattgaccg cgcccgccgc atgaccatgc cgattctcgg gcgaatggag caggtgttgc gtgaacatca tgccattcgc gatgccatct cggcccgtga tgtccatgcc gcgcgcgaag ccatgaaaca tcatttaagt gctgttttgc cggatattga cgagttgcgc aaaagccgtc cggactattt tgcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat