BrmeB.18114.a

L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16)

CENTER ID: BrmeB.18114.a
ORGANISM: Brucella melitensis ATCC 23457
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.18114.a.B1.GE37805 Full length( BrmeB.18114.a ) 1 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.18114.a.B1.PS02059 Full length( BrmeB.18114.a ) 1 405

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel14466_2431
BV-BRC: fig|546272.3.peg.2431
RefSeq: YP_002733995.1
UniProt: C0RKA8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAPFSVQPP ESALRPLDGI TVVSLEHAIA APFATRQLAD LGARVIKVER PGVGDFARAY DDRVNGMASH FVWCNRSKES LAIDLKHPDA RGILHDLVSK ADVLVQNLAP GAAARLGLSA EALRADCPHL IVCDISGYGS DGPYRDRKAY DLLIQSEAGF LSVTGTPDEQ AKAGISIADI AAGMYAYSGI LSALLMRERT GQGSHIEVSM LEALGEWMGF PLYYTYGGKE PPPRTGASHA TIFPYGPFPT GDGKTVMLGL QNEREWAIFC EKVLHQPALA QDERFVSNVL RVRNRDALTE IIVASFAASN AEAITMQLDE AGIANARVRT MAEVWDHPQL QARGRWTEIT TPAGQVPALL PPAFPNGMEP RMDPVPALGA HNISILTELG LDEAQIAALR EAGAI
NT Sequence
ATGACTGCAC CCTTCTCAGT CCAGCCGCCA GAAAGCGCCC TCCGCCCTCT CGACGGGATA ACGGTCGTCT CTCTGGAACA CGCCATAGCG GCCCCTTTCG CCACGCGCCA GCTTGCCGAT CTGGGCGCAC GCGTCATCAA GGTCGAGCGG CCTGGGGTCG GCGATTTCGC CCGCGCCTAT GATGATCGCG TCAATGGTAT GGCGTCACAT TTTGTCTGGT GCAACCGTTC CAAGGAGAGC CTTGCGATTG ATCTGAAACA CCCGGATGCC AGAGGCATTC TGCATGATCT CGTGTCGAAA GCGGATGTTC TTGTTCAAAA CCTTGCACCG GGCGCGGCAG CGCGGCTGGG GCTTTCAGCG GAGGCGCTGC GGGCGGACTG TCCGCACCTT ATCGTTTGTG ATATTTCCGG CTATGGGTCG GATGGTCCCT ATCGTGACCG TAAGGCTTAC GATCTGCTGA TCCAGAGCGA AGCAGGCTTC CTGTCCGTCA CAGGTACGCC CGACGAGCAG GCGAAAGCCG GTATTTCCAT CGCCGATATT GCAGCCGGCA TGTATGCCTA TAGCGGTATT CTCTCTGCCC TGCTGATGCG TGAGCGCACT GGTCAAGGCA GCCATATCGA GGTGTCGATG CTGGAGGCAC TGGGTGAATG GATGGGCTTT CCGCTATACT ATACTTATGG TGGCAAGGAA CCGCCTCCGC GCACAGGCGC CAGCCACGCC ACCATCTTCC CCTATGGGCC ATTTCCGACC GGCGACGGCA AAACAGTGAT GCTGGGTCTG CAAAACGAAC GTGAATGGGC GATCTTTTGT GAAAAAGTGT TGCATCAGCC CGCTCTTGCC CAGGACGAAC GATTTGTCTC GAACGTCTTG CGGGTTCGTA ACCGTGATGC TCTCACCGAA ATTATTGTTG CATCCTTTGC CGCCAGCAAT GCTGAAGCGA TTACCATGCA ATTGGACGAA GCCGGCATCG CCAATGCGCG CGTGCGCACC ATGGCCGAAG TGTGGGATCA CCCTCAATTG CAGGCACGCG GACGCTGGAC AGAAATTACT ACGCCCGCCG GTCAGGTGCC TGCATTGCTT CCGCCAGCAT TTCCCAACGG TATGGAGCCG CGAATGGATC CCGTTCCCGC GCTTGGCGCT CACAATATCA GTATCCTGAC AGAGCTTGGC CTTGATGAGG CCCAGATTGC GGCTTTGCGC GAGGCGGGGG CAATA
Details for BrmeB.18114.a.B1.GE37805
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/13/2014
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 77
Validated AA Sequence
MASHFVWCNR SKESLAIDLK HPDARGILHD LVSKADVLVQ NLAPGAAARL GLSAEALRAD CPHLIVCDIS GYGSDGPYRD RKAYDLLIQS EAGFLSVTGT PDEQAKAGIS IADIAAGMYA YSGILSALLM RERTGQGSHI EVSMLEALGE WMGFPLYYTY GGKEPPPRTG ASHATIFPYG PFPTGDGKTV MLGLQNEREW AIFCEKVLHQ PALAQDERFV SNVLRVRNRD ALTEIIVASF AASNAEAITM QLDEAGIANA RVRTMAEVWD HPQLQARGRW TEITTPAGQV PALLPPAFPN GMEPRMDPVP ALGAHNISIL T
Validated NT Sequence
ctctgtcagg atactgatat tgtgagcgcc aagcgcggga acgggatcca ttcgcggctc cataccgttg ggaaatgctg gcggaagcaa tgcaggcacc tgaccggcgg gcgtagtaat ttctgtccag cgtccgcgtg cctgcaattg agggtgatcc cacacttcgg ccatggtgcg cacgcgcgca ttggcgatgc cggcttcgtc caattgcatg gtaatcgctt cagcattgct ggcggcaaag gatgcaacaa taatttcggt gagagcatca cggttacgaa cccgcaagac gttcgagaca aatcgttcgt cctgggcaag agcgggctga tgcaacactt tttcacaaaa gatcgcccat tcacgttcgt tttgcagacc cagcatcact gttttgccgt cgccggtcgg aaatggccca taggggaaga tggtggcgtg gctggcgcct gtgcgcggag gcggttcctt gccaccataa gtatagtata gcggaaagcc catccattca cccagtgcct ccagcatcga cacctcgata tggctgcctt gaccagtgcg ctcacgcatc agcagggcag agagaatacc gctataggca tacatgccgg ctgcaatatc ggcgatggaa ataccggctt tcgcctgctc gtcgggcgta cctgtgacgg acaggaagcc tgcttcgctc tggatcagca gatcgtaagc cttacggtca cgatagggac catccgaccc atagccggaa atatcacaaa cgataaggtg cggacagtcc gcccgcagcg cctccgctga aagccccagc cgcgctgccg cgcccggtgc aaggttttga acaagaacat ccgctttcga cacgagatca tgcagaatgc ctctggcatc cgggtgtttc agatcaatcg caaggctctc cttggaacgg ttgcaccaga caaaatgtga cgccatacca ttgacgcgat catcataggc gcgggcgaaa tcgccgaccc caggccgctc gaccttgatg acgcgtgcgc ccagatcggc aagctggcgc gtggcgaaag gggccgctat ggcgtgttcc agagagacga ccgttatccc gtcgagaggg cggagggcgc tttctggcgg ctggactgag aagggtgcag tc
Expected Protein Sequence
MAHHHHHHMT APFSVQPPES ALRPLDGITV VSLEHAIAAP FATRQLADLG ARVIKVERPG VGDFARAYDD RVNGMASHFV WCNRSKESLA IDLKHPDARG ILHDLVSKAD VLVQNLAPGA AARLGLSAEA LRADCPHLIV CDISGYGSDG PYRDRKAYDL LIQSEAGFLS VTGTPDEQAK AGISIADIAA GMYAYSGILS ALLMRERTGQ GSHIEVSMLE ALGEWMGFPL YYTYGGKEPP PRTGASHATI FPYGPFPTGD GKTVMLGLQN EREWAIFCEK VLHQPALAQD ERFVSNVLRV RNRDALTEII VASFAASNAE AITMQLDEAG IANARVRTMA EVWDHPQLQA RGRWTEITTP AGQVPALLPP AFPNGMEPRM DPVPALGAHN ISILTELGLD EAQIAALREA GAI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg cacccttctc agtccagccg ccagaaagcg ccctccgccc tctcgacggg ataacggtcg tctctctgga acacgccata gcggcccctt tcgccacgcg ccagcttgcc gatctgggcg cacgcgtcat caaggtcgag cggcctgggg tcggcgattt cgcccgcgcc tatgatgatc gcgtcaatgg tatggcgtca cattttgtct ggtgcaaccg ttccaaggag agccttgcga ttgatctgaa acacccggat gccagaggca ttctgcatga tctcgtgtcg aaagcggatg ttcttgttca aaaccttgca ccgggcgcgg cagcgcggct ggggctttca gcggaggcgc tgcgggcgga ctgtccgcac cttatcgttt gtgatatttc cggctatggg tcggatggtc cctatcgtga ccgtaaggct tacgatctgc tgatccagag cgaagcaggc ttcctgtccg tcacaggtac gcccgacgag caggcgaaag ccggtatttc catcgccgat attgcagccg gcatgtatgc ctatagcggt attctctctg ccctgctgat gcgtgagcgc actggtcaag gcagccatat cgaggtgtcg atgctggagg cactgggtga atggatgggc tttccgctat actatactta tggtggcaag gaaccgcctc cgcgcacagg cgccagccac gccaccatct tcccctatgg gccatttccg accggcgacg gcaaaacagt gatgctgggt ctgcaaaacg aacgtgaatg ggcgatcttt tgtgaaaaag tgttgcatca gcccgctctt gcccaggacg aacgatttgt ctcgaacgtc ttgcgggttc gtaaccgtga tgctctcacc gaaattattg ttgcatcctt tgccgccagc aatgctgaag cgattaccat gcaattggac gaagccggca tcgccaatgc gcgcgtgcgc accatggccg aagtgtggga tcaccctcaa ttgcaggcac gcggacgctg gacagaaatt actacgcccg ccggtcaggt gcctgcattg cttccgccag catttcccaa cggtatggag ccgcgaatgg atcccgttcc cgcgcttggc gctcacaata tcagtatcct gacagagctt ggccttgatg aggcccagat tgcggctttg cgcgaggcgg gggcaatatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BrmeB.18114.a.B1.PS02059
PURIFICATION DATe: 5/22/2014
CONCENTRATION: 39.23mg/ml
OBSERVED MW: 42kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 77
Protocol Notes
notes unavailable
Validated AA Sequence
MASHFVWCNR SKESLAIDLK HPDARGILHD LVSKADVLVQ NLAPGAAARL GLSAEALRAD CPHLIVCDIS GYGSDGPYRD RKAYDLLIQS EAGFLSVTGT PDEQAKAGIS IADIAAGMYA YSGILSALLM RERTGQGSHI EVSMLEALGE WMGFPLYYTY GGKEPPPRTG ASHATIFPYG PFPTGDGKTV MLGLQNEREW AIFCEKVLHQ PALAQDERFV SNVLRVRNRD ALTEIIVASF AASNAEAITM QLDEAGIANA RVRTMAEVWD HPQLQARGRW TEITTPAGQV PALLPPAFPN GMEPRMDPVP ALGAHNISIL T
Validated NT Sequence
ctctgtcagg atactgatat tgtgagcgcc aagcgcggga acgggatcca ttcgcggctc cataccgttg ggaaatgctg gcggaagcaa tgcaggcacc tgaccggcgg gcgtagtaat ttctgtccag cgtccgcgtg cctgcaattg agggtgatcc cacacttcgg ccatggtgcg cacgcgcgca ttggcgatgc cggcttcgtc caattgcatg gtaatcgctt cagcattgct ggcggcaaag gatgcaacaa taatttcggt gagagcatca cggttacgaa cccgcaagac gttcgagaca aatcgttcgt cctgggcaag agcgggctga tgcaacactt tttcacaaaa gatcgcccat tcacgttcgt tttgcagacc cagcatcact gttttgccgt cgccggtcgg aaatggccca taggggaaga tggtggcgtg gctggcgcct gtgcgcggag gcggttcctt gccaccataa gtatagtata gcggaaagcc catccattca cccagtgcct ccagcatcga cacctcgata tggctgcctt gaccagtgcg ctcacgcatc agcagggcag agagaatacc gctataggca tacatgccgg ctgcaatatc ggcgatggaa ataccggctt tcgcctgctc gtcgggcgta cctgtgacgg acaggaagcc tgcttcgctc tggatcagca gatcgtaagc cttacggtca cgatagggac catccgaccc atagccggaa atatcacaaa cgataaggtg cggacagtcc gcccgcagcg cctccgctga aagccccagc cgcgctgccg cgcccggtgc aaggttttga acaagaacat ccgctttcga cacgagatca tgcagaatgc ctctggcatc cgggtgtttc agatcaatcg caaggctctc cttggaacgg ttgcaccaga caaaatgtga cgccatacca ttgacgcgat catcataggc gcgggcgaaa tcgccgaccc caggccgctc gaccttgatg acgcgtgcgc ccagatcggc aagctggcgc gtggcgaaag gggccgctat ggcgtgttcc agagagacga ccgttatccc gtcgagaggg cggagggcgc tttctggcgg ctggactgag aagggtgcag tc
Expressed Protein Sequence
MAHHHHHHMT APFSVQPPES ALRPLDGITV VSLEHAIAAP FATRQLADLG ARVIKVERPG VGDFARAYDD RVNGMASHFV WCNRSKESLA IDLKHPDARG ILHDLVSKAD VLVQNLAPGA AARLGLSAEA LRADCPHLIV CDISGYGSDG PYRDRKAYDL LIQSEAGFLS VTGTPDEQAK AGISIADIAA GMYAYSGILS ALLMRERTGQ GSHIEVSMLE ALGEWMGFPL YYTYGGKEPP PRTGASHATI FPYGPFPTGD GKTVMLGLQN EREWAIFCEK VLHQPALAQD ERFVSNVLRV RNRDALTEII VASFAASNAE AITMQLDEAG IANARVRTMA EVWDHPQLQA RGRWTEITTP AGQVPALLPP AFPNGMEPRM DPVPALGAHN ISILTELGLD EAQIAALREA GAI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTG CACCCTTCTC AGTCCAGCCG CCAGAAAGCG CCCTCCGCCC TCTCGACGGG ATAACGGTCG TCTCTCTGGA ACACGCCATA GCGGCCCCTT TCGCCACGCG CCAGCTTGCC GATCTGGGCG CACGCGTCAT CAAGGTCGAG CGGCCTGGGG TCGGCGATTT CGCCCGCGCC TATGATGATC GCGTCAATGG TATGGCGTCA CATTTTGTCT GGTGCAACCG TTCCAAGGAG AGCCTTGCGA TTGATCTGAA ACACCCGGAT GCCAGAGGCA TTCTGCATGA TCTCGTGTCG AAAGCGGATG TTCTTGTTCA AAACCTTGCA CCGGGCGCGG CAGCGCGGCT GGGGCTTTCA GCGGAGGCGC TGCGGGCGGA CTGTCCGCAC CTTATCGTTT GTGATATTTC CGGCTATGGG TCGGATGGTC CCTATCGTGA CCGTAAGGCT TACGATCTGC TGATCCAGAG CGAAGCAGGC TTCCTGTCCG TCACAGGTAC GCCCGACGAG CAGGCGAAAG CCGGTATTTC CATCGCCGAT ATTGCAGCCG GCATGTATGC CTATAGCGGT ATTCTCTCTG CCCTGCTGAT GCGTGAGCGC ACTGGTCAAG GCAGCCATAT CGAGGTGTCG ATGCTGGAGG CACTGGGTGA ATGGATGGGC TTTCCGCTAT ACTATACTTA TGGTGGCAAG GAACCGCCTC CGCGCACAGG CGCCAGCCAC GCCACCATCT TCCCCTATGG GCCATTTCCG ACCGGCGACG GCAAAACAGT GATGCTGGGT CTGCAAAACG AACGTGAATG GGCGATCTTT TGTGAAAAAG TGTTGCATCA GCCCGCTCTT GCCCAGGACG AACGATTTGT CTCGAACGTC TTGCGGGTTC GTAACCGTGA TGCTCTCACC GAAATTATTG TTGCATCCTT TGCCGCCAGC AATGCTGAAG CGATTACCAT GCAATTGGAC GAAGCCGGCA TCGCCAATGC GCGCGTGCGC ACCATGGCCG AAGTGTGGGA TCACCCTCAA TTGCAGGCAC GCGGACGCTG GACAGAAATT ACTACGCCCG CCGGTCAGGT GCCTGCATTG CTTCCGCCAG CATTTCCCAA CGGTATGGAG CCGCGAATGG ATCCCGTTCC CGCGCTTGGC GCTCACAATA TCAGTATCCT GACAGAGCTT GGCCTTGATG AGGCCCAGAT TGCGGCTTTG CGCGAGGCGG GGGCAATATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT