BrmeB.17757.a

Choline-binding lipoprotein

CENTER ID: BrmeB.17757.a
ORGANISM: Brucella melitensis ATCC 23457
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.17757.a.B2.GE36974 Mature protein( BrmeB.17757.a ) 22 319
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel14466_3472
BV-BRC: fig|546272.3.peg.3472
RefSeq: YP_002734899.1
UniProt: C0RMW2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRRTFLAMS LALTFLPSVA LADNIPVRVG YIADYWGTSI TAIASEKGLW EKHGLDADTR VFTNGPIQVQ ALGAGSLDFG YIGPGALWLP ASGKAKIVAI NSVGFSDRVI AQEGFKSMAD LKGKKIGVPE GTSGDMLLRL ALGKAGMKLD DVQVIKMDPS TVVSAFASKQ IDAAGIWYPL IGTIKEHVPG MVELAANSDF FPDKTFPSAF IARNEIVAEN PEAVKRMIAV IEEAEDFRTA NPEQSVDITA KFLKVDKANL ETEAKNGKSL TSEELVKLTR DGSVNGWLSG MADMFVTFGK LKSPLDPKDY YLADYFTAK
NT Sequence
ATGAAAAGAC GCACTTTTCT TGCAATGAGC CTGGCATTAA CATTCCTGCC CTCCGTCGCC CTTGCCGACA ATATTCCGGT GCGCGTTGGT TATATCGCGG ATTATTGGGG CACCAGTATC ACCGCGATTG CAAGCGAGAA GGGGCTTTGG GAGAAGCACG GTCTTGATGC GGACACGCGG GTATTCACCA ACGGCCCCAT TCAGGTGCAG GCACTTGGTG CGGGCAGCCT TGATTTCGGC TATATCGGAC CGGGTGCGCT ATGGTTGCCG GCAAGCGGCA AGGCCAAGAT CGTGGCCATC AATTCGGTGG GTTTTTCAGA CCGGGTGATC GCGCAGGAGG GCTTCAAGTC GATGGCCGAC CTGAAGGGCA AGAAGATCGG TGTTCCCGAA GGCACCTCTG GCGATATGCT GCTCCGGCTC GCTCTCGGTA AGGCGGGCAT GAAGCTGGAT GATGTTCAGG TCATCAAGAT GGACCCGTCT ACGGTCGTAT CGGCCTTTGC CTCCAAACAA ATCGACGCCG CAGGCATCTG GTATCCATTG ATCGGCACGA TCAAGGAGCA TGTTCCCGGT ATGGTGGAAC TGGCTGCCAA TAGCGACTTC TTTCCGGATA AAACCTTTCC GAGTGCATTC ATCGCGCGCA ACGAGATCGT GGCCGAGAAC CCGGAGGCGG TGAAGCGGAT GATCGCGGTC ATCGAGGAAG CCGAGGACTT CCGCACCGCC AACCCGGAAC AGTCCGTCGA TATTACGGCA AAATTTCTCA AGGTCGATAA GGCCAATCTG GAAACGGAAG CCAAAAACGG AAAGTCGCTG ACGAGTGAGG AACTGGTGAA GCTCACCCGT GATGGATCGG TCAATGGATG GCTGTCGGGC ATGGCCGATA TGTTCGTAAC CTTCGGCAAG CTGAAAAGCC CGCTCGACCC GAAAGACTAT TATCTGGCCG ATTATTTCAC GGCAAAA
Details for BrmeB.17757.a.B2.GE36974
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 33kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA DNIPVRVGYI ADYWGTSITA IASEKGLWEK HGLDADTRVF TNGPIQVQAL GAGSLDFGYI GPGALWLPAS GKAKIVAINS VGFSDRVIAQ EGFKSMADLK GKKIGVPEGT SGDMLLRLAL GKAGMKLDDV QVIKMDPSTV VSAFASKQID AAGIWYPLIG TIKEHVPGMV ELAANSDFFP DKTFPSAFIA RNEIVAENPE AVKRMIAVIE EAEDFRTANP EQSVDITAKF LKVDKANLET EAKNGKSLTS EELVKLTRDG SVNGWLSGMA DMFVTFGKLK SPLDPKDYYL ADYFTAK
Validated NT Sequence
tcttactcac ttattttgcc gtgaaataat cggccagata atagtctttc gggtcgagcg ggcttttcag cttgccgaag gttacgaaca tatcggccat gcccgacagc catccattga ccgatccatc acgggtgagc ttcaccagtt cctcactcgt cagcgacttt ccgtttttgg cttccgtttc cagattggcc ttatcgacct tgagaaattt tgccgtaata tcgacggact gttccgggtt ggcggtgcgg aagtcctcgg cttcctcgat gaccgcgatc atccgcttca ccgcctccgg gttctcggcc acgatctcgt tgcgcgcgat gaatgcactc ggaaaggttt tatccggaaa gaagtcgcta ttggcagcca gttccaccat accgggaaca tgctccttga tcgtgccgat caatggatac cagatgcctg cggcgtcgat ttgtttggag gcaaaggccg atacgaccgt agacgggtcc atcttgatga cctgaacatc atccagcttc atgcccgcct taccgagagc gagccggagc agcatatcgc cagaggtgcc ttcgggaaca ccgatcttct tgcccttcag gtcggccatc gacttgaagc cctcctgcgc gatcacccgg tctgaaaaac ccaccgaatt gatggccacg atcttggcct tgccgcttgc cggcaaccat agcgcacccg gtccgatata gccgaaatca aggctgcccg caccaagtgc ctgcacctga atggggccgt tggtgaatac ccgcgtgtcc gcatcaagac cgtgcttctc ccaaagcccc ttctcgcttg caatcgcggt gatactggtg ccccaataat ccgcgatata accaacgcgc accggaatat tgtcggccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHAD NIPVRVGYIA DYWGTSITAI ASEKGLWEKH GLDADTRVFT NGPIQVQALG AGSLDFGYIG PGALWLPASG KAKIVAINSV GFSDRVIAQE GFKSMADLKG KKIGVPEGTS GDMLLRLALG KAGMKLDDVQ VIKMDPSTVV SAFASKQIDA AGIWYPLIGT IKEHVPGMVE LAANSDFFPD KTFPSAFIAR NEIVAENPEA VKRMIAVIEE AEDFRTANPE QSVDITAKFL KVDKANLETE AKNGKSLTSE ELVKLTRDGS VNGWLSGMAD MFVTFGKLKS PLDPKDYYLA DYFTAK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgaca atattccggt gcgcgttggt tatatcgcgg attattgggg caccagtatc accgcgattg caagcgagaa ggggctttgg gagaagcacg gtcttgatgc ggacacgcgg gtattcacca acggccccat tcaggtgcag gcacttggtg cgggcagcct tgatttcggc tatatcggac cgggtgcgct atggttgccg gcaagcggca aggccaagat cgtggccatc aattcggtgg gtttttcaga ccgggtgatc gcgcaggagg gcttcaagtc gatggccgac ctgaagggca agaagatcgg tgttcccgaa ggcacctctg gcgatatgct gctccggctc gctctcggta aggcgggcat gaagctggat gatgttcagg tcatcaagat ggacccgtct acggtcgtat cggcctttgc ctccaaacaa atcgacgccg caggcatctg gtatccattg atcggcacga tcaaggagca tgttcccggt atggtggaac tggctgccaa tagcgacttc tttccggata aaacctttcc gagtgcattc atcgcgcgca acgagatcgt ggccgagaac ccggaggcgg tgaagcggat gatcgcggtc atcgaggaag ccgaggactt ccgcaccgcc aacccggaac agtccgtcga tattacggca aaatttctca aggtcgataa ggccaatctg gaaacggaag ccaaaaacgg aaagtcgctg acgagtgagg aactggtgaa gctcacccgt gatggatcgg tcaatggatg gctgtcgggc atggccgata tgttcgtaac cttcggcaag ctgaaaagcc cgctcgaccc gaaagactat tatctggccg attatttcac ggcaaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at