BrmeB.17460.a

2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.-)

CENTER ID:	BrmeB.17460.a
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.17460.a.B1.GE36968	Full length( BrmeB.17460.a )		1	269

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|546272.3.peg.3441
RefSeq:	YP_002734872.1
UniProt:	C0RMT5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPAPRNQFKA ALKQGRAQIG LWQALANPYT VEICAGAGYD WLLLDGEHAP NDVPLLVSQL QAMTGSASHP VVRPPVGETW IVKQLLDIGA QTLLIPMIES REQAEAMVKA MRYPPHGVRG VGSALARASA FNRIPDYLQT ANDEVCLLLQ VESRAGLANL DAIATTEGVD GVFIGPADLA ADMGHLGKPG AQEVQDAVRD ALQRIIGHGK AAGILTGDLS LARHYLDLGA TFVAIGNDVT LLANATTRLL ADFKTTEPGR PASPDARVY

NT Sequence

ATGCCTGCTC CTCGAAATCA GTTCAAGGCC GCGCTCAAGC AGGGCCGCGC CCAGATCGGC CTGTGGCAGG CGCTCGCCAA TCCCTATACG GTCGAAATCT GTGCCGGAGC CGGATATGAC TGGCTGCTGC TCGATGGCGA ACATGCGCCC AATGATGTGC CTCTTCTGGT TTCACAGCTT CAGGCCATGA CAGGTTCAGC CTCGCATCCG GTCGTGCGGC CGCCTGTCGG CGAAACGTGG ATCGTCAAGC AATTGCTCGA TATCGGCGCG CAGACCCTGC TGATCCCCAT GATTGAAAGC CGCGAACAGG CCGAAGCCAT GGTAAAAGCC ATGCGCTATC CGCCGCATGG GGTGCGTGGT GTCGGTTCCG CTCTGGCGCG GGCTTCTGCC TTCAACCGTA TCCCCGATTA TCTACAGACG GCCAATGACG AGGTTTGTCT TCTGTTGCAG GTGGAAAGCC GCGCGGGGCT TGCCAATCTT GATGCAATTG CCACGACCGA AGGCGTGGAC GGCGTCTTCA TCGGCCCTGC CGATCTTGCT GCCGATATGG GGCATCTGGG CAAACCGGGC GCGCAAGAGG TGCAGGACGC TGTGCGCGAC GCACTTCAAC GCATCATCGG GCACGGCAAG GCGGCGGGCA TTCTCACGGG CGACCTTTCG CTTGCCCGTC ATTATCTCGA CCTTGGCGCA ACCTTTGTCG CCATCGGCAA TGATGTGACC TTGCTCGCGA ATGCCACCAC TAGGCTGCTT GCCGACTTCA AGACCACTGA GCCGGGCAGG CCAGCCAGCC CGGACGCAAG GGTCTAT

Details for BrmeB.17460.a.B1.GE36968

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMP APRNQFKAAL KQGRAQIGLW QALANPYTVE ICAGAGYDWL LLDGEHAPND VPLLVSQLQA MTGSASHPVV RPPVGETWIV KQLLDIGAQT LLIPMIESRE QAEAMVKAMR YPPHGVRGVG SALARASAFN RIPDYLQTAN DEVCLLLQVE SRAGLANLDA IATTEGVDGV FIGPADLAAD MGHLGKPGAQ EVQDAVRDAL QRIIGHGKAA GILTGDLSLA RHYLDLGATF VAIGNDVTLL ANATTRLLAD FKTTEPGRPA SPDARVY

Validated NT Sequence

atcttactca cttaatagac ccttgcgtcc gggctggctg gcctgcccgg ctcagtggtc ttgaagtcgg caagcagcct agtggtggca ttcgcgagca aggtcacatc attgccgatg gcgacaaagg ttgcgccaag gtcgagataa tgacgggcaa gcgaaaggtc gcccgtgaga atgcccgccg ccttgccgtg cccgatgatg cgttgaagtg cgtcgcgcac agcgtcctgc acctcttgcg cgcccggttt gcccagatgc cccatatcgg cagcaagatc ggcagggccg atgaagacgc cgtccacgcc ttcggtcgtg gcaattgcat caagattggc aagccccgcg cggctttcca cctgcaacag aagacaaacc tcgtcattgg ccgtctgtag ataatcgggg atacggttga aggcagaagc ccgcgccaga gcggaaccga caccacgcac cccatgcggc ggatagcgca tggcttttac catggcttcg gcctgttcgc ggctttcaat catggggatc agcagggtct gcgcgccgat atcgagcaat tgcttgacga tccacgtttc gccgacaggc ggccgcacga ccggatgcga ggctgaacct gtcatggcct gaagctgtga aaccagaaga ggcacatcat tgggcgcatg ttcgccatcg agcagcagcc agtcatatcc ggctccggca cagatttcga ccgtataggg attggcgagc gcctgccaca ggccgatctg ggcgcggccc tgcttgagcg cggccttgaa ctgatttcga ggagcaggca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcctg ctcctcgaaa tcagttcaag gccgcgctca agcagggccg cgcccagatc ggcctgtggc aggcgctcgc caatccctat acggtcgaaa tctgtgccgg agccggatat gactggctgc tgctcgatgg cgaacatgcg cccaatgatg tgcctcttct ggtttcacag cttcaggcca tgacaggttc agcctcgcat ccggtcgtgc ggccgcctgt cggcgaaacg tggatcgtca agcaattgct cgatatcggc gcgcagaccc tgctgatccc catgattgaa agccgcgaac aggccgaagc catggtaaaa gccatgcgct atccgccgca tggggtgcgt ggtgtcggtt ccgctctggc gcgggcttct gccttcaacc gtatccccga ttatctacag acggccaatg acgaggtttg tcttctgttg caggtggaaa gccgcgcggg gcttgccaat cttgatgcaa ttgccacgac cgaaggcgtg gacggcgtct tcatcggccc tgccgatctt gctgccgata tggggcatct gggcaaaccg ggcgcgcaag aggtgcagga cgctgtgcgc gacgcacttc aacgcatcat cgggcacggc aaggcggcgg gcattctcac gggcgacctt tcgcttgccc gtcattatct cgaccttggc gcaacctttg tcgccatcgg caatgatgtg accttgctcg cgaatgccac cactaggctg cttgccgact tcaagaccac tgagccgggc aggccagcca gcccggacgc aagggtctat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat