SSGCID: Target Page

BrmeB.17394.a

Putative hemolysin

CENTER ID:	BrmeB.17394.a
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.17394.a.B1.GE36967	Full length( BrmeB.17394.a )		1	297

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_0162
BV-BRC:	fig\|546272.3.peg.162
RefSeq:	YP_002731915.1
UniProt:	C0RGK3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTVLLGMGQQ IIDDTVMSGL EAQQALFASN GDAIILGRIG SLEVRLANSR AAIEAAQELR FRVFFEEMGA RKETIEAVEQ RDADRFDTIC DHLLVYDTAL PVPEHQQIVG TYRLMRNEQA EKALGFYSAD EYDVQRLKLS RPNLRLLELG RSCVKPEYRS KRTVELLWQG AWAYCRRHSI DVMFGCASFH GAVPAAHALG LSFLHHNCRA TDDWDVRALP HRYLAMDLMP KEAINNKVAL FSMPPLVKGY LRLGAMIGDG AVIDEAFGTT DVFIILPIER ISSRYISYYG AEANRFV

NT Sequence

ATGACAGTAC TGCTTGGAAT GGGCCAGCAA ATCATTGATG ATACGGTCAT GTCAGGCTTA GAAGCACAAC AGGCGCTGTT CGCCTCCAAT GGCGACGCCA TCATTCTGGG ACGCATCGGG TCGCTGGAAG TGCGGCTTGC GAATTCCCGC GCGGCAATCG AAGCTGCGCA GGAGCTTCGT TTTCGCGTCT TCTTTGAAGA GATGGGCGCC CGCAAGGAAA CCATCGAAGC GGTGGAGCAG CGCGATGCTG ATCGCTTCGA TACGATCTGC GATCATCTTC TGGTCTATGA TACGGCCCTG CCCGTCCCCG AACACCAGCA AATCGTCGGC ACCTATCGCC TGATGCGCAA CGAGCAGGCC GAAAAGGCGC TGGGTTTCTA TTCGGCGGAC GAATATGACG TGCAACGCCT CAAACTGTCG CGCCCCAACC TTCGCCTGCT GGAACTCGGC CGTTCCTGCG TGAAACCGGA ATATCGTTCC AAGCGCACCG TCGAGCTTTT GTGGCAGGGC GCATGGGCCT ATTGCCGCCG ACACTCCATC GACGTGATGT TCGGCTGTGC CTCTTTCCAC GGGGCCGTGC CTGCAGCCCA CGCGCTGGGG CTTTCCTTCC TGCATCACAA TTGCCGCGCC ACCGACGATT GGGACGTTCG CGCCCTGCCG CACCGCTACC TGGCGATGGA CCTGATGCCA AAGGAAGCGA TCAACAACAA AGTTGCGCTT TTCTCGATGC CGCCATTGGT GAAAGGCTAT CTGCGCCTTG GGGCAATGAT CGGGGATGGC GCCGTGATCG ATGAGGCCTT CGGCACCACC GATGTCTTCA TTATCCTACC CATCGAGCGC ATTTCCAGCC GTTACATTAG CTACTACGGC GCAGAAGCAA ACCGCTTTGT C

Details for BrmeB.17394.a.B1.GE36967

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	34kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT VLLGMGQQII DDTVMSGLEA QQALFASNGD AIILGRIGSL EVRLANSRAA IEAAQELRFR VFFEEMGARK ETIEAVEQRD ADRFDTICDH LLVYDTALPV PEHQQIVGTY RLMRNEQAEK ALGFYSADEY DVQRLKLSRP NLRLLELGRS CVKPEYRSKR TVELLWQGAW AYCRRHSIDV MFGCASFHGA VPAAHALGLS FLHHNCRATD DWDVRALPHR YLAMDLMPKE AINNKVALFS MPPLVKGYLR LGAMIGDGAV IDEAFGTTDV FIILPIERIS XRYISYYGAX QTALSX

Validated NT Sequence

ttgtnnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatgacagta ctgcttggaa tgggccagca aatcattgat gatacggtca tgtcaggctt agaagcacaa caggcgctgt tcgcctccaa tggcgacgcc atcattctgg gacgcatcgg gtcgctggaa gtgcggcttg cgaattcccg cgcggcaatc gaagctgcgc aggagcttcg ttttcgcgtc ttctttgaag agatgggcgc ccgcaaggaa accatcgaag cggtggagca gcgcgatgct gatcgcttcg atacgatctg cgatcatctt ctggtctatg atacggccct gcccgtcccc gaacaccagc aaatcgtcgg cacctatcgc ctgatgcgca acgagcaggc cgaaaaggcg ctgggtttct attcggcgga cgaatatgac gtgcaacgcc tcaaactgtc gcgccccaac cttcgcctgc tggaactcgg ccgttcctgc gtgaaaccgg aatatcgttc caagcgcacc gtcgagcttt tgtggcaggg cgcatgggcc tattgccgcc gacactccat cgacgtgatg ttcggctgtg cctctttcca cggggccgtg cctgcagccc acgcgctggg gctttccttc ctgcatcaca attgccgcgc caccgacgat tgggacgttc gcgccctgcc gcaccgctac ctggcgatgg acctgatgcc aaaggaagcg atcaacaaca aagttgcgct tttctcgatg ccgccattgg tgaaaggcta tctgcgcctt ggggcaatga tcggggatgg cgccgtgatc gatgaggcct tcggcaccac cgatgtcttc attatcctac ccatcgagcg catttccngc cgttacatta gctactacgg cgcanaacaa accgctttgt ctaantgagn annnaggann nnnnnnnnnn nnncnannnn nnnnnnannn nnnnnnnnnn nc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacag tactgcttgg aatgggccag caaatcattg atgatacggt catgtcaggc ttagaagcac aacaggcgct gttcgcctcc aatggcgacg ccatcattct gggacgcatc gggtcgctgg aagtgcggct tgcgaattcc cgcgcggcaa tcgaagctgc gcaggagctt cgttttcgcg tcttctttga agagatgggc gcccgcaagg aaaccatcga agcggtggag cagcgcgatg ctgatcgctt cgatacgatc tgcgatcatc ttctggtcta tgatacggcc ctgcccgtcc ccgaacacca gcaaatcgtc ggcacctatc gcctgatgcg caacgagcag gccgaaaagg cgctgggttt ctattcggcg gacgaatatg acgtgcaacg cctcaaactg tcgcgcccca accttcgcct gctggaactc ggccgttcct gcgtgaaacc ggaatatcgt tccaagcgca ccgtcgagct tttgtggcag ggcgcatggg cctattgccg ccgacactcc atcgacgtga tgttcggctg tgcctctttc cacggggccg tgcctgcagc ccacgcgctg gggctttcct tcctgcatca caattgccgc gccaccgacg attgggacgt tcgcgccctg ccgcaccgct acctggcgat ggacctgatg ccaaaggaag cgatcaacaa caaagttgcg cttttctcga tgccgccatt ggtgaaaggc tatctgcgcc ttggggcaat gatcggggat ggcgccgtga tcgatgaggc cttcggcacc accgatgtct tcattatcct acccatcgag cgcatttcca gccgttacat tagctactac ggcgcagaag caaaccgctt tgtctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat