SSGCID: Target Page

BrmeB.17389.a

Methionyl-tRNA formyltransferase (EC 2.1.2.9)

CENTER ID:	BrmeB.17389.a
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.17389.a.B1.GE36966	Full length( BrmeB.17389.a )		1	306

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.17389.a.B1.PS01910	Full length( BrmeB.17389.a )		1	306

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_3307
BV-BRC:	fig\|546272.3.peg.3307
RefSeq:	YP_002734760.1
UniProt:	C0RMH3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRVVFMGTPE FSVPILTAII GHGYEVVAAY TQPPRPAGRR GLELTRSPVH EKAEQFGIPV FTPKSLKGAE EQDVFASLKA DVAIVVAYGL LLPKAILDAP RLGCYNGHAS LLPRWRGAAP IQRAIMAGDA ETGMMIMKMD EGLDTGPVAM AEKVAITPDM TAGELHDRLS MIGADLMIRA LGALERESLA LQPQAEEGVT YAAKIDKAEA RIDWSKPAKD VHNSIRGLSP FPGAWCEMEI NGAVERVKLQ RSTLGEGSGA PGTVLDDRLT IACGEGAVRL ATLQRSGGKP LPAQEFLRGQ RVTKVL

NT Sequence

ATGCGTGTTG TTTTCATGGG GACGCCGGAA TTTTCCGTGC CGATCCTCAC CGCCATCATC GGTCACGGTT ATGAGGTCGT TGCAGCCTAT ACGCAGCCGC CGCGCCCGGC AGGCCGCAGA GGTCTGGAAC TCACCAGATC GCCGGTGCAT GAAAAAGCAG AGCAATTCGG CATTCCGGTT TTCACGCCGA AGAGCCTCAA GGGCGCGGAA GAGCAGGATG TTTTCGCAAG CCTTAAAGCC GATGTGGCGA TTGTCGTGGC CTATGGGCTT TTGCTGCCGA AGGCCATTCT CGATGCGCCG CGGCTTGGCT GCTATAACGG CCATGCGTCG CTTTTGCCGC GTTGGCGCGG TGCAGCGCCC ATCCAGCGCG CTATTATGGC AGGCGATGCG GAAACCGGCA TGATGATCAT GAAGATGGAT GAGGGGCTTG ATACCGGGCC TGTCGCCATG GCCGAAAAGG TGGCCATCAC GCCGGATATG ACGGCGGGCG AACTGCATGA TCGCCTGAGC ATGATTGGCG CTGACCTGAT GATCCGCGCG CTGGGCGCGC TTGAGCGCGA AAGCCTTGCC TTGCAGCCGC AGGCGGAAGA AGGCGTTACT TATGCCGCAA AGATCGACAA GGCGGAGGCC CGCATCGACT GGTCGAAGCC TGCAAAAGAC GTGCACAATA GCATTCGCGG CCTGTCACCC TTTCCGGGCG CGTGGTGCGA GATGGAAATC AACGGTGCTG TCGAGCGCGT GAAGTTGCAG CGTTCCACGC TGGGCGAAGG TTCGGGCGCG CCGGGCACGG TGCTGGATGA TCGCCTGACG ATTGCCTGCG GCGAGGGCGC GGTGCGGCTT GCCACGCTGC AACGTTCCGG CGGAAAACCC TTGCCTGCAC AAGAGTTTCT GCGTGGGCAG CGCGTCACGA AGGTTCTC

Details for BrmeB.17389.a.B1.GE36966

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR VVFMGTPEFS VPILTAIIGH GYEVVAAYTQ PPRPAGRRGL ELTRSPVHEK AEQFGIPVFT PKSLKGAEEQ DVFASLKADV AIVVAYGLLL PKAILDAPRL GCYNGHASLL PRWRGAAPIQ RAIMAGDAET GMMIMKMDEG LDTGPVAMAE KVAITPDMTA GELHDRLSMI GADLMIRALG ALERESLALQ PQAEEGVTYA AKIDKAEARI DWSKPAKDVH NSIRGLSPFP GAWCEMEING AVERVKLQRS TLGEGSGAPG TVLDDRLTIA CGEGAVRLAT LQRSGGKPLP AQEFLRGQRV TKVL

Validated NT Sequence

tactcactta gagaaccttc gtgacgcgct gcccacgcag aaactcttgt gcaggcaagg gttttccgcc ggaacgttgc agcgtggcaa gccgcaccgc gccctcgccg caggcaatcg tcaggcgatc atccagcacc gtgcccggcg cgcccgaacc ttcgcccagc gtggaacgct gcaacttcac gcgctcgaca gcaccgttga tttccatctc gcaccacgcg cccggaaagg gtgacaggcc gcgaatgcta ttgtgcacgt cttttgcagg cttcgaccag tcgatgcggg cctccgcctt gtcgatcttt gcggcataag taacgccttc ttccgcctgc ggctgcaagg caaggctttc gcgctcaagc gcgcccagcg cgcggatcat caggtcagcg ccaatcatgc tcaggcgatc atgcagttcg cccgccgtca tatccggcgt gatggccacc ttttcggcca tggcgacagg cccggtatca agcccctcat ccatcttcat gatcatcatg ccggtttccg catcgcctgc cataatagcg cgctggatgg gcgctgcacc gcgccaacgc ggcaaaagcg acgcatggcc gttatagcag ccaagccgcg gcgcatcgag aatggccttc ggcagcaaaa gcccataggc cacgacaatc gccacatcgg ctttaaggct tgcgaaaaca tcctgctctt ccgcgccctt gaggctcttc ggcgtgaaaa ccggaatgcc gaattgctct gctttttcat gcaccggcga tctggtgagt tccagacctc tgcggcctgc cgggcgcggc ggctgcgtat aggctgcaac gacctcataa ccgtgaccga tgatggcggt gaggatcggc acggaaaatt ccggcgtccc catgaaaaca acacgcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtg ttgttttcat ggggacgccg gaattttccg tgccgatcct caccgccatc atcggtcacg gttatgaggt cgttgcagcc tatacgcagc cgccgcgccc ggcaggccgc agaggtctgg aactcaccag atcgccggtg catgaaaaag cagagcaatt cggcattccg gttttcacgc cgaagagcct caagggcgcg gaagagcagg atgttttcgc aagccttaaa gccgatgtgg cgattgtcgt ggcctatggg cttttgctgc cgaaggccat tctcgatgcg ccgcggcttg gctgctataa cggccatgcg tcgcttttgc cgcgttggcg cggtgcagcg cccatccagc gcgctattat ggcaggcgat gcggaaaccg gcatgatgat catgaagatg gatgaggggc ttgataccgg gcctgtcgcc atggccgaaa aggtggccat cacgccggat atgacggcgg gcgaactgca tgatcgcctg agcatgattg gcgctgacct gatgatccgc gcgctgggcg cgcttgagcg cgaaagcctt gccttgcagc cgcaggcgga agaaggcgtt acttatgccg caaagatcga caaggcggag gcccgcatcg actggtcgaa gcctgcaaaa gacgtgcaca atagcattcg cggcctgtca ccctttccgg gcgcgtggtg cgagatggaa atcaacggtg ctgtcgagcg cgtgaagttg cagcgttcca cgctgggcga aggttcgggc gcgccgggca cggtgctgga tgatcgcctg acgattgcct gcggcgaggg cgcggtgcgg cttgccacgc tgcaacgttc cggcggaaaa cccttgcctg cacaagagtt tctgcgtggg cagcgcgtca cgaaggttct ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BrmeB.17389.a.B1.PS01910

PURIFICATION DATe:	10/3/2013
CONCENTRATION:	44mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGTG TTGTTTTCAT GGGGACGCCG GAATTTTCCG TGCCGATCCT CACCGCCATC ATCGGTCACG GTTATGAGGT CGTTGCAGCC TATACGCAGC CGCCGCGCCC GGCAGGCCGC AGAGGTCTGG AACTCACCAG ATCGCCGGTG CATGAAAAAG CAGAGCAATT CGGCATTCCG GTTTTCACGC CGAAGAGCCT CAAGGGCGCG GAAGAGCAGG ATGTTTTCGC AAGCCTTAAA GCCGATGTGG CGATTGTCGT GGCCTATGGG CTTTTGCTGC CGAAGGCCAT TCTCGATGCG CCGCGGCTTG GCTGCTATAA CGGCCATGCG TCGCTTTTGC CGCGTTGGCG CGGTGCAGCG CCCATCCAGC GCGCTATTAT GGCAGGCGAT GCGGAAACCG GCATGATGAT CATGAAGATG GATGAGGGGC TTGATACCGG GCCTGTCGCC ATGGCCGAAA AGGTGGCCAT CACGCCGGAT ATGACGGCGG GCGAACTGCA TGATCGCCTG AGCATGATTG GCGCTGACCT GATGATCCGC GCGCTGGGCG CGCTTGAGCG CGAAAGCCTT GCCTTGCAGC CGCAGGCGGA AGAAGGCGTT ACTTATGCCG CAAAGATCGA CAAGGCGGAG GCCCGCATCG ACTGGTCGAA GCCTGCAAAA GACGTGCACA ATAGCATTCG CGGCCTGTCA CCCTTTCCGG GCGCGTGGTG CGAGATGGAA ATCAACGGTG CTGTCGAGCG CGTGAAGTTG CAGCGTTCCA CGCTGGGCGA AGGTTCGGGC GCGCCGGGCA CGGTGCTGGA TGATCGCCTG ACGATTGCCT GCGGCGAGGG CGCGGTGCGG CTTGCCACGC TGCAACGTTC CGGCGGAAAA CCCTTGCCTG CACAAGAGTT TCTGCGTGGG CAGCGCGTCA CGAAGGTTCT CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT