BrmeB.17387.a

Citrate lyase beta chain (EC 4.1.3.6)

CENTER ID: BrmeB.17387.a
ORGANISM: Brucella melitensis ATCC 23457
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.17387.a.B1.GE36964 Full length( BrmeB.17387.a ) 1 331
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.17387.a.B1.PS01930 Full length( BrmeB.17387.a ) 1 331

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel14466_3142
BV-BRC: fig|546272.3.peg.3142
RefSeq: YP_002734615.1
UniProt: C0RM28

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALNKMSVVL PPVSFQTISG LGPQLFSVLA LWGCSVHKSG MKTIVRPRRS VLFVPAANTR ALEKSLTLSA DCVIYDLEDS VAPEAKAAAR EALAAHLSAH PKVAFERIVR VNAADTPWGR DDVAAVAKMA VNAVLLPKVE RPQDVIEAAN HLDRQDADPA MGVWAMIETP RGILNVDEIA VLGHRSAVRL ACFVVGPNDI ARETGLRSQP GRPYLAPWLM QIVLAARAGG IAVLDGVYND FRDSAGFEAE CAQGAAMGFD GKTLIHPGQI EAANRAFSPT EEEVAHARAV REIFARPENA GKGVVSLDGQ MVERLHLEMA ERILAKAGIN L
NT Sequence
ATGGCGCTCA ATAAAATGTC CGTCGTCCTG CCGCCGGTCA GCTTTCAAAC TATTTCAGGG CTTGGCCCCC AACTATTCTC GGTGCTTGCG CTGTGGGGCT GTTCGGTGCA CAAATCGGGC ATGAAAACAA TTGTTCGTCC CCGTCGTTCC GTCCTGTTTG TTCCTGCCGC AAATACGCGG GCATTGGAAA AATCGCTGAC GCTTTCCGCC GATTGCGTGA TCTATGATCT GGAGGATTCG GTTGCGCCGG AGGCGAAGGC TGCGGCCCGC GAGGCACTTG CAGCGCATCT TTCGGCCCAT CCGAAAGTTG CTTTCGAGCG GATCGTGCGG GTCAATGCGG CAGACACCCC ATGGGGCAGG GACGATGTTG CGGCTGTGGC GAAAATGGCT GTAAACGCCG TCCTTTTGCC GAAGGTGGAG CGCCCGCAGG ATGTGATCGA GGCCGCCAAT CATCTCGACC GGCAGGATGC CGATCCCGCC ATGGGCGTAT GGGCCATGAT CGAAACACCG CGCGGCATTT TGAATGTCGA TGAGATTGCT GTTCTGGGGC ATCGCTCGGC GGTGCGGCTT GCCTGTTTTG TGGTAGGGCC GAACGATATC GCGCGGGAAA CCGGGCTTCG CTCACAGCCG GGACGCCCTT ATCTGGCGCC GTGGCTGATG CAGATTGTGC TTGCGGCGCG CGCTGGCGGC ATTGCGGTGC TGGATGGCGT TTATAATGAT TTTCGTGATA GCGCTGGCTT CGAGGCCGAA TGTGCGCAGG GCGCGGCCAT GGGTTTTGAC GGCAAGACGC TGATCCATCC GGGCCAGATC GAAGCGGCCA ATCGCGCCTT CTCACCGACG GAGGAGGAAG TGGCCCATGC GCGCGCAGTG CGCGAGATTT TTGCCCGGCC GGAAAATGCC GGCAAGGGCG TCGTTTCGCT GGACGGGCAA ATGGTCGAGC GCCTGCATCT GGAAATGGCC GAACGGATTC TCGCGAAGGC TGGTATAAAT CTG
Details for BrmeB.17387.a.B1.GE36964
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MSVVLPPVSF QTISGLGPQL FSVLALWGCS VHKSGMKTIV RPRRSVLFVP AANTRALEKS LTLSADCVIY DLEDSVAPEA KAAAREALAA HLSAHPKVAF ERIVRVNAAD TPWGRDDVAA VAKMAVNAVL LPKVERPQDV IEAANHLDRQ DADPAMGVWA MIETPRGILN VDEIAVLGHR SAVRLACFVV GPNDIARETG LRSQPGRPYL APWLMQIVLA ARAGGIAVLD GVYNDFRDSA GFEAECAQGA AMGFDGKTLI HPGQIEAANR AFSPTEEEVA HARAVREIFA RPENAGKGVV SLDGQMVERL HLEMAERILA KAGINL
Validated NT Sequence
tcttactcac ttacagattt ataccagcct tcgcgagaat ccgttcggcc atttccagat gcaggcgctc gaccatttgc ccgtccagcg aaacgacgcc cttgccggca ttttccggcc gggcaaaaat ctcgcgcact gcgcgcgcat gggccacttc ctcctccgtc ggtgagaagg cgcgattggc cgcttcgatc tggcccggat ggatcagcgt cttgccgtca aaacccatgg ccgcgccctg cgcacattcg gcctcgaagc cagcgctatc acgaaaatca ttataaacgc catccagcac cgcaatgccg ccagcgcgcg ccgcaagcac aatctgcatc agccacggcg ccagataagg gcgtcccggc tgtgagcgaa gcccggtttc ccgcgcgata tcgttcggcc ctaccacaaa acaggcaagc cgcaccgccg agcgatgccc cagaacagca atctcatcga cattcaaaat gccgcgcggt gtttcgatca tggcccatac gcccatggcg ggatcggcat cctgccggtc gagatgattg gcggcctcga tcacatcctg cgggcgctcc accttcggca aaaggacggc gtttacagcc attttcgcca cagccgcaac atcgtccctg ccccatgggg tgtctgccgc attgacccgc acgatccgct cgaaagcaac tttcggatgg gccgaaagat gcgctgcaag tgcctcgcgg gccgcagcct tcgcctccgg cgcaaccgaa tcctccagat catagatcac gcaatcggcg gaaagcgtca gcgatttttc caatgcccgc gtatttgcgg caggaacaaa caggacggaa cgacggggac gaacaattgt tttcatgccc gatttgtgca ccgaacagcc ccacagcgca agcaccgaga atagttgggg gccaagccct gaaatagttt gaaagctgac cggcggcagg acgacggaca ttttattg
Expected Protein Sequence
MAHHHHHHMA LNKMSVVLPP VSFQTISGLG PQLFSVLALW GCSVHKSGMK TIVRPRRSVL FVPAANTRAL EKSLTLSADC VIYDLEDSVA PEAKAAAREA LAAHLSAHPK VAFERIVRVN AADTPWGRDD VAAVAKMAVN AVLLPKVERP QDVIEAANHL DRQDADPAMG VWAMIETPRG ILNVDEIAVL GHRSAVRLAC FVVGPNDIAR ETGLRSQPGR PYLAPWLMQI VLAARAGGIA VLDGVYNDFR DSAGFEAECA QGAAMGFDGK TLIHPGQIEA ANRAFSPTEE EVAHARAVRE IFARPENAGK GVVSLDGQMV ERLHLEMAER ILAKAGINL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgc tcaataaaat gtccgtcgtc ctgccgccgg tcagctttca aactatttca gggcttggcc cccaactatt ctcggtgctt gcgctgtggg gctgttcggt gcacaaatcg ggcatgaaaa caattgttcg tccccgtcgt tccgtcctgt ttgttcctgc cgcaaatacg cgggcattgg aaaaatcgct gacgctttcc gccgattgcg tgatctatga tctggaggat tcggttgcgc cggaggcgaa ggctgcggcc cgcgaggcac ttgcagcgca tctttcggcc catccgaaag ttgctttcga gcggatcgtg cgggtcaatg cggcagacac cccatggggc agggacgatg ttgcggctgt ggcgaaaatg gctgtaaacg ccgtcctttt gccgaaggtg gagcgcccgc aggatgtgat cgaggccgcc aatcatctcg accggcagga tgccgatccc gccatgggcg tatgggccat gatcgaaaca ccgcgcggca ttttgaatgt cgatgagatt gctgttctgg ggcatcgctc ggcggtgcgg cttgcctgtt ttgtggtagg gccgaacgat atcgcgcggg aaaccgggct tcgctcacag ccgggacgcc cttatctggc gccgtggctg atgcagattg tgcttgcggc gcgcgctggc ggcattgcgg tgctggatgg cgtttataat gattttcgtg atagcgctgg cttcgaggcc gaatgtgcgc agggcgcggc catgggtttt gacggcaaga cgctgatcca tccgggccag atcgaagcgg ccaatcgcgc cttctcaccg acggaggagg aagtggccca tgcgcgcgca gtgcgcgaga tttttgcccg gccggaaaat gccggcaagg gcgtcgtttc gctggacggg caaatggtcg agcgcctgca tctggaaatg gccgaacgga ttctcgcgaa ggctggtata aatctgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BrmeB.17387.a.B1.PS01930
PURIFICATION DATe: 10/23/2013
CONCENTRATION: 22.3mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MSVVLPPVSF QTISGLGPQL FSVLALWGCS VHKSGMKTIV RPRRSVLFVP AANTRALEKS LTLSADCVIY DLEDSVAPEA KAAAREALAA HLSAHPKVAF ERIVRVNAAD TPWGRDDVAA VAKMAVNAVL LPKVERPQDV IEAANHLDRQ DADPAMGVWA MIETPRGILN VDEIAVLGHR SAVRLACFVV GPNDIARETG LRSQPGRPYL APWLMQIVLA ARAGGIAVLD GVYNDFRDSA GFEAECAQGA AMGFDGKTLI HPGQIEAANR AFSPTEEEVA HARAVREIFA RPENAGKGVV SLDGQMVERL HLEMAERILA KAGINL
Validated NT Sequence
tcttactcac ttacagattt ataccagcct tcgcgagaat ccgttcggcc atttccagat gcaggcgctc gaccatttgc ccgtccagcg aaacgacgcc cttgccggca ttttccggcc gggcaaaaat ctcgcgcact gcgcgcgcat gggccacttc ctcctccgtc ggtgagaagg cgcgattggc cgcttcgatc tggcccggat ggatcagcgt cttgccgtca aaacccatgg ccgcgccctg cgcacattcg gcctcgaagc cagcgctatc acgaaaatca ttataaacgc catccagcac cgcaatgccg ccagcgcgcg ccgcaagcac aatctgcatc agccacggcg ccagataagg gcgtcccggc tgtgagcgaa gcccggtttc ccgcgcgata tcgttcggcc ctaccacaaa acaggcaagc cgcaccgccg agcgatgccc cagaacagca atctcatcga cattcaaaat gccgcgcggt gtttcgatca tggcccatac gcccatggcg ggatcggcat cctgccggtc gagatgattg gcggcctcga tcacatcctg cgggcgctcc accttcggca aaaggacggc gtttacagcc attttcgcca cagccgcaac atcgtccctg ccccatgggg tgtctgccgc attgacccgc acgatccgct cgaaagcaac tttcggatgg gccgaaagat gcgctgcaag tgcctcgcgg gccgcagcct tcgcctccgg cgcaaccgaa tcctccagat catagatcac gcaatcggcg gaaagcgtca gcgatttttc caatgcccgc gtatttgcgg caggaacaaa caggacggaa cgacggggac gaacaattgt tttcatgccc gatttgtgca ccgaacagcc ccacagcgca agcaccgaga atagttgggg gccaagccct gaaatagttt gaaagctgac cggcggcagg acgacggaca ttttattg
Expressed Protein Sequence
MAHHHHHHMA LNKMSVVLPP VSFQTISGLG PQLFSVLALW GCSVHKSGMK TIVRPRRSVL FVPAANTRAL EKSLTLSADC VIYDLEDSVA PEAKAAAREA LAAHLSAHPK VAFERIVRVN AADTPWGRDD VAAVAKMAVN AVLLPKVERP QDVIEAANHL DRQDADPAMG VWAMIETPRG ILNVDEIAVL GHRSAVRLAC FVVGPNDIAR ETGLRSQPGR PYLAPWLMQI VLAARAGGIA VLDGVYNDFR DSAGFEAECA QGAAMGFDGK TLIHPGQIEA ANRAFSPTEE EVAHARAVRE IFARPENAGK GVVSLDGQMV ERLHLEMAER ILAKAGINL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGC TCAATAAAAT GTCCGTCGTC CTGCCGCCGG TCAGCTTTCA AACTATTTCA GGGCTTGGCC CCCAACTATT CTCGGTGCTT GCGCTGTGGG GCTGTTCGGT GCACAAATCG GGCATGAAAA CAATTGTTCG TCCCCGTCGT TCCGTCCTGT TTGTTCCTGC CGCAAATACG CGGGCATTGG AAAAATCGCT GACGCTTTCC GCCGATTGCG TGATCTATGA TCTGGAGGAT TCGGTTGCGC CGGAGGCGAA GGCTGCGGCC CGCGAGGCAC TTGCAGCGCA TCTTTCGGCC CATCCGAAAG TTGCTTTCGA GCGGATCGTG CGGGTCAATG CGGCAGACAC CCCATGGGGC AGGGACGATG TTGCGGCTGT GGCGAAAATG GCTGTAAACG CCGTCCTTTT GCCGAAGGTG GAGCGCCCGC AGGATGTGAT CGAGGCCGCC AATCATCTCG ACCGGCAGGA TGCCGATCCC GCCATGGGCG TATGGGCCAT GATCGAAACA CCGCGCGGCA TTTTGAATGT CGATGAGATT GCTGTTCTGG GGCATCGCTC GGCGGTGCGG CTTGCCTGTT TTGTGGTAGG GCCGAACGAT ATCGCGCGGG AAACCGGGCT TCGCTCACAG CCGGGACGCC CTTATCTGGC GCCGTGGCTG ATGCAGATTG TGCTTGCGGC GCGCGCTGGC GGCATTGCGG TGCTGGATGG CGTTTATAAT GATTTTCGTG ATAGCGCTGG CTTCGAGGCC GAATGTGCGC AGGGCGCGGC CATGGGTTTT GACGGCAAGA CGCTGATCCA TCCGGGCCAG ATCGAAGCGG CCAATCGCGC CTTCTCACCG ACGGAGGAGG AAGTGGCCCA TGCGCGCGCA GTGCGCGAGA TTTTTGCCCG GCCGGAAAAT GCCGGCAAGG GCGTCGTTTC GCTGGACGGG CAAATGGTCG AGCGCCTGCA TCTGGAAATG GCCGAACGGA TTCTCGCGAA GGCTGGTATA AATCTGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T