BrmeB.17377.d

Maltose operon transcriptional repressor MalR, LacI family

CENTER ID: BrmeB.17377.d
ORGANISM: Brucella melitensis ATCC 23457
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.17377.d.B1.GE36963 Full length( BrmeB.17377.d ) 1 338
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel14466_2572
BV-BRC: fig|546272.3.peg.2572
RefSeq: YP_002734119.1
UniProt: C0RKN2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLREFAKHM GLSATTVSRA LSGYPEVAES TRKRVMEEAI RLGYRPNVNA VRLVTGRAGA IGVVMGRNSE FHFAEFMRGM AERLGQDEVD ILVSPTSPTG NDDEVQLCHR LATSGRVDAV IVTSPRPNDE RIRMLHKLGM PFLVHGRSQI DIPHAWLDID NESAVYHSIA HLLDLGHKRI AMINGRHGFT FSLHRDAGYR KALESRGIDF DPDLVEHGDF TDEIGYCLAR KLLERNPRPT AFVAGSMMTA LGIYRAARSF GLTIGKDISV IAHDDVISFL SAGNMVPSLT ATRSSMRAAG KRSADLLMDI LDGRAPTEIH ELWPVELILR ESATRVPE
NT Sequence
ATGAAACTCC GTGAATTCGC CAAACATATG GGCCTTTCCG CCACGACCGT CAGCCGGGCG CTGAGCGGCT ATCCCGAAGT GGCGGAAAGC ACGCGCAAGC GCGTGATGGA AGAGGCCATC CGTCTTGGCT ATCGCCCCAA CGTGAATGCG GTGCGGCTGG TAACGGGCCG CGCGGGCGCA ATCGGCGTCG TGATGGGCCG CAACAGCGAG TTTCACTTTG CCGAATTCAT GCGCGGCATG GCCGAACGGC TGGGGCAGGA CGAAGTGGAC ATTCTCGTCA GCCCCACCTC GCCGACCGGC AATGACGACG AGGTGCAGCT TTGTCACCGG CTGGCAACGA GCGGACGGGT GGATGCCGTT ATCGTCACTT CCCCCAGGCC GAATGATGAA CGCATCCGCA TGTTGCACAA GCTCGGCATG CCGTTTCTGG TCCACGGGCG TTCGCAGATC GACATTCCCC ATGCATGGCT TGATATCGAC AATGAGAGCG CGGTCTATCA TTCCATCGCA CATCTGCTCG ATCTTGGCCA CAAGCGGATT GCGATGATCA ACGGGCGCCA TGGCTTCACC TTCTCGCTGC ACCGCGACGC TGGCTATCGT AAGGCGCTTG AAAGCCGGGG AATCGACTTT GATCCCGACC TGGTGGAACA TGGCGATTTC ACCGATGAAA TCGGCTATTG CCTCGCCCGC AAGCTTCTGG AACGCAATCC ACGTCCAACG GCATTCGTCG CAGGCTCCAT GATGACCGCG CTCGGCATCT ACCGTGCCGC CCGCTCCTTC GGCCTTACGA TCGGCAAGGA TATTTCCGTC ATCGCCCATG ACGACGTGAT CTCGTTCCTC AGCGCCGGTA ATATGGTGCC GTCGCTGACT GCGACACGCT CTTCAATGCG CGCGGCTGGC AAGCGATCCG CCGACCTTTT GATGGATATC CTCGATGGGC GCGCGCCCAC CGAAATCCAT GAATTATGGC CGGTTGAACT GATCCTTCGC GAATCCGCCA CCCGTGTTCC CGAA
Details for BrmeB.17377.d.B1.GE36963
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 38kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MGLSATTVSR ALSGYPEVAE STRKRVMEEA IRLGYRPNVN AVRLVTGRAG AIGVVMGRNS EFHFAEFMRG MAERLGQDEV DILVSPTSPT GNDDEVQLCH RLATSGRVDA VIVTSPRPND ERIRMLHKLG MPFLVHGRSQ IDIPHAWLDI DNESAVYHSI AHLLDLGHKR IAMINGRHGF TFSLHRDAGY RKALESRGID FDPDLVEHGD FTDEIGYCLA RKLLERNPRP TAFVAGSMMT ALGIYRAARS FGLTIGKDIS VIAHDDVISF LSAGNMVPSL TATRSSMRAA GKRSADLLMD ILDGRAPTEI HELWPVELIL RESATRVPE
Validated NT Sequence
acttattcgg gaacacgggt ggcggattcg cgaaggatca gttcaaccgg ccataattca tggatttcgg tgggcgcgcg cccatcgagg atatccatca aaaggtcggc ggatcgcttg ccagccgcgc gcattgaaga gcgtgtcgca gtcagcgacg gcaccatatt accggcgctg aggaacgaga tcacgtcgtc atgggcgatg acggaaatat ccttgccgat cgtaaggccg aaggagcggg cggcacggta gatgccgagc gcggtcatca tggagcctgc gacgaatgcc gttggacgtg gattgcgttc cagaagcttg cgggcgaggc aatagccgat ttcatcggtg aaatcgccat gttccaccag gtcgggatca aagtcgattc cccggctttc aagcgcctta cgatagccag cgtcgcggtg cagcgagaag gtgaagccat ggcgcccgtt gatcatcgca atccgcttgt ggccaagatc gagcagatgt gcgatggaat gatagaccgc gctctcattg tcgatatcaa gccatgcatg gggaatgtcg atctgcgaac gcccgtggac cagaaacggc atgccgagct tgtgcaacat gcggatgcgt tcatcattcg gcctggggga agtgacgata acggcatcca cccgtccgct cgttgccagc cggtgacaaa gctgcacctc gtcgtcattg ccggtcggcg aggtggggct gacgagaatg tccacttcgt cctgccccag ccgttcggcc atgccgcgca tgaattcggc aaagtgaaac tcgctgttgc ggcccatcac gacgccgatt gcgcccgcgc ggcccgttac cagccgcacc gcattcacgt tggggcgata gccaagacgg atggcctctt ccatcacgcg cttgcgcgtg ctttccgcca cttcgggata gccgctcagc gcccggctga cggtcgtggc ggaaaggccc atatgtttgg cgaattcacg g
Expected Protein Sequence
MAHHHHHHMK LREFAKHMGL SATTVSRALS GYPEVAESTR KRVMEEAIRL GYRPNVNAVR LVTGRAGAIG VVMGRNSEFH FAEFMRGMAE RLGQDEVDIL VSPTSPTGND DEVQLCHRLA TSGRVDAVIV TSPRPNDERI RMLHKLGMPF LVHGRSQIDI PHAWLDIDNE SAVYHSIAHL LDLGHKRIAM INGRHGFTFS LHRDAGYRKA LESRGIDFDP DLVEHGDFTD EIGYCLARKL LERNPRPTAF VAGSMMTALG IYRAARSFGL TIGKDISVIA HDDVISFLSA GNMVPSLTAT RSSMRAAGKR SADLLMDILD GRAPTEIHEL WPVELILRES ATRVPE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac tccgtgaatt cgccaaacat atgggccttt ccgccacgac cgtcagccgg gcgctgagcg gctatcccga agtggcggaa agcacgcgca agcgcgtgat ggaagaggcc atccgtcttg gctatcgccc caacgtgaat gcggtgcggc tggtaacggg ccgcgcgggc gcaatcggcg tcgtgatggg ccgcaacagc gagtttcact ttgccgaatt catgcgcggc atggccgaac ggctggggca ggacgaagtg gacattctcg tcagccccac ctcgccgacc ggcaatgacg acgaggtgca gctttgtcac cggctggcaa cgagcggacg ggtggatgcc gttatcgtca cttcccccag gccgaatgat gaacgcatcc gcatgttgca caagctcggc atgccgtttc tggtccacgg gcgttcgcag atcgacattc cccatgcatg gcttgatatc gacaatgaga gcgcggtcta tcattccatc gcacatctgc tcgatcttgg ccacaagcgg attgcgatga tcaacgggcg ccatggcttc accttctcgc tgcaccgcga cgctggctat cgtaaggcgc ttgaaagccg gggaatcgac tttgatcccg acctggtgga acatggcgat ttcaccgatg aaatcggcta ttgcctcgcc cgcaagcttc tggaacgcaa tccacgtcca acggcattcg tcgcaggctc catgatgacc gcgctcggca tctaccgtgc cgcccgctcc ttcggcctta cgatcggcaa ggatatttcc gtcatcgccc atgacgacgt gatctcgttc ctcagcgccg gtaatatggt gccgtcgctg actgcgacac gctcttcaat gcgcgcggct ggcaagcgat ccgccgacct tttgatggat atcctcgatg ggcgcgcgcc caccgaaatc catgaattat ggccggttga actgatcctt cgcgaatccg ccacccgtgt tcccgaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at