BrmeB.17377.c

Ribitol operon repressor

CENTER ID: BrmeB.17377.c
ORGANISM: Brucella melitensis ATCC 23457
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.17377.c.B1.GE36962 Full length( BrmeB.17377.c ) 1 351
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.17377.c.B1.PS01927 Full length( BrmeB.17377.c ) 1 351

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel14466_2527
BV-BRC: fig|546272.3.peg.2527
RefSeq: YP_002734083.1
UniProt: C0RKJ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHISRKKATI YDLSVLSGSS PSTVSAVLNG TWRKRRISQA TAEAILQLAD QHAYTANRQA QGLRHSRSGL VGLLLPLHDN RFFSAMAQIF EAHVRARSFC PVVVSASRDP EEERETAETL ISYAIDELFI CGATDPDGLH ELCEKAALPH VNIDLPGSRA PSVTTDNFAG GKMLTAAIIE YFGEEAPLQP EDLCLFGGRN DESTRQRICG FHQVKQTVLG ADPEGCVFKT GYSPANTLVA LNDYRARHGR LPRGLFVNSS INLEGLLRFM AEHPGEPLND IVIGCYDYDP FASFLPFPVI SILQDVEGMI AKAFDLMERR VTEPELHLIM PKLIAPRSAI TGPLDSFKDI K
NT Sequence
ATGCACATCA GCAGGAAAAA GGCGACGATC TATGATCTTT CCGTTTTGTC GGGAAGCTCG CCTTCGACTG TGAGCGCCGT GCTGAACGGC ACATGGCGGA AGCGGCGTAT CAGTCAGGCT ACGGCGGAGG CCATATTGCA ACTGGCCGAT CAGCATGCCT ATACCGCCAA CCGGCAGGCA CAAGGCCTGC GTCATTCCCG CTCCGGCCTG GTGGGGCTTT TGCTCCCGTT GCATGACAAT CGCTTTTTCT CCGCTATGGC GCAGATTTTT GAGGCACATG TACGCGCGCG CAGCTTTTGC CCCGTGGTGG TGAGCGCAAG CCGCGACCCG GAAGAAGAGC GCGAGACGGC GGAAACCCTC ATTTCCTATG CCATTGACGA GCTGTTTATT TGTGGCGCCA CTGATCCGGA CGGCTTGCAT GAGCTCTGCG AAAAAGCGGC CCTGCCACAT GTGAATATCG ACCTGCCTGG CTCCAGGGCT CCTTCCGTCA CCACGGATAA TTTCGCGGGC GGCAAGATGC TCACTGCGGC CATCATCGAA TATTTTGGCG AAGAGGCGCC TTTGCAGCCT GAAGATCTTT GTCTTTTTGG CGGACGAAAC GATGAATCGA CCCGCCAGCG CATTTGCGGT TTCCATCAGG TGAAGCAGAC CGTGCTGGGG GCCGACCCGG AAGGCTGCGT GTTCAAGACC GGCTATTCGC CTGCAAATAC TCTGGTTGCG CTCAACGATT ATCGCGCGCG GCATGGCAGG CTGCCGCGCG GTCTCTTCGT CAATTCCTCC ATCAATCTGG AAGGGTTGCT ACGCTTCATG GCAGAGCATC CGGGCGAGCC GCTCAATGAT ATTGTCATTG GCTGTTACGA CTATGATCCC TTCGCCTCGT TCCTGCCCTT TCCGGTTATT TCTATCCTTC AGGATGTGGA AGGCATGATT GCAAAAGCCT TCGACCTGAT GGAACGGCGC GTGACGGAAC CGGAATTGCA CCTCATCATG CCGAAGCTTA TTGCGCCGCG TTCCGCTATC ACCGGGCCAC TCGATAGTTT CAAGGATATC AAA
Details for BrmeB.17377.c.B1.GE36962
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MHISRKKATI YDLSVLSGSS PSTVSAVLNG TWRKRRISQA TAEAILQLAD QHAYTANRQA QGLRHSRSGL VGLLLPLHDN RFFSAMAQIF EAHVRARSFC PVVVSASRDP EEERETAETL ISYAIDELFI CGATDPDGLH ELCEKAALPH VNIDLPGSRA PSVTTDNFAG GKMLTAAIIE YFGEEAPLQP EDLCLFGGRN DESTRQRICG FHQVKQTVLG ADPEGCVFKT GYSPANTLVA LNDYRARHGR LPRGLFVNSS INLEGLLRFM AEHPGEPLND IVIGCYDYDP FASFLPFPVI SILQDVEGMI AKAFDLMERR VTEPELHLIM PKLIAPRSAI TGPLDSFKDI K
Validated NT Sequence
tatttgatat ccttgaaact atcgagtggc ccggtgatag cggaacgcgg cgcaataagc ttcggcatga tgaggtgcaa ttccggttcc gtcacgcgcc gttccatcag gtcgaaggct tttgcaatca tgccttccac atcctgaagg atagaaataa ccggaaaggg caggaacgag gcgaagggat catagtcgta acagccaatg acaatatcat tgagcggctc gcccggatgc tctgccatga agcgtagcaa cccttccaga ttgatggagg aattgacgaa gagaccgcgc ggcagcctgc catgccgcgc gcgataatcg ttgagcgcaa ccagagtatt tgcaggcgaa tagccggtct tgaacacgca gccttccggg tcggccccca gcacggtctg cttcacctga tggaaaccgc aaatgcgctg gcgggtcgat tcatcgtttc gtccgccaaa aagacaaaga tcttcaggct gcaaaggcgc ctcttcgcca aaatattcga tgatggccgc agtgagcatc ttgccgcccg cgaaattatc cgtggtgacg gaaggagccc tggagccagg caggtcgata ttcacatgtg gcagggccgc tttttcgcag agctcatgca agccgtccgg atcagtggcg ccacaaataa acagctcgtc aatggcatag gaaatgaggg tttccgccgt ctcgcgctct tcttccgggt cgcggcttgc gctcaccacc acggggcaaa agctgcgcgc gcgtacatgt gcctcaaaaa tctgcgccat agcggagaaa aagcgattgt catgcaacgg gagcaaaagc cccaccaggc cggagcggga atgacgcagg ccttgtgcct gccggttggc ggtataggca tgctgatcgg ccagttgcaa tatggcctcc gccgtagcct gactgatacg ccgcttccgc catgtgccgt tcagcacggc gctcacagtc gaaggcgagc ttcccgacaa aacggaaaga tcatagatcg tcgccttttt cctgctgatg tgcatatggt ggtggtggtg
Expected Protein Sequence
MAHHHHHHMH ISRKKATIYD LSVLSGSSPS TVSAVLNGTW RKRRISQATA EAILQLADQH AYTANRQAQG LRHSRSGLVG LLLPLHDNRF FSAMAQIFEA HVRARSFCPV VVSASRDPEE ERETAETLIS YAIDELFICG ATDPDGLHEL CEKAALPHVN IDLPGSRAPS VTTDNFAGGK MLTAAIIEYF GEEAPLQPED LCLFGGRNDE STRQRICGFH QVKQTVLGAD PEGCVFKTGY SPANTLVALN DYRARHGRLP RGLFVNSSIN LEGLLRFMAE HPGEPLNDIV IGCYDYDPFA SFLPFPVISI LQDVEGMIAK AFDLMERRVT EPELHLIMPK LIAPRSAITG PLDSFKDIK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaca tcagcaggaa aaaggcgacg atctatgatc tttccgtttt gtcgggaagc tcgccttcga ctgtgagcgc cgtgctgaac ggcacatggc ggaagcggcg tatcagtcag gctacggcgg aggccatatt gcaactggcc gatcagcatg cctataccgc caaccggcag gcacaaggcc tgcgtcattc ccgctccggc ctggtggggc ttttgctccc gttgcatgac aatcgctttt tctccgctat ggcgcagatt tttgaggcac atgtacgcgc gcgcagcttt tgccccgtgg tggtgagcgc aagccgcgac ccggaagaag agcgcgagac ggcggaaacc ctcatttcct atgccattga cgagctgttt atttgtggcg ccactgatcc ggacggcttg catgagctct gcgaaaaagc ggccctgcca catgtgaata tcgacctgcc tggctccagg gctccttccg tcaccacgga taatttcgcg ggcggcaaga tgctcactgc ggccatcatc gaatattttg gcgaagaggc gcctttgcag cctgaagatc tttgtctttt tggcggacga aacgatgaat cgacccgcca gcgcatttgc ggtttccatc aggtgaagca gaccgtgctg ggggccgacc cggaaggctg cgtgttcaag accggctatt cgcctgcaaa tactctggtt gcgctcaacg attatcgcgc gcggcatggc aggctgccgc gcggtctctt cgtcaattcc tccatcaatc tggaagggtt gctacgcttc atggcagagc atccgggcga gccgctcaat gatattgtca ttggctgtta cgactatgat cccttcgcct cgttcctgcc ctttccggtt atttctatcc ttcaggatgt ggaaggcatg attgcaaaag ccttcgacct gatggaacgg cgcgtgacgg aaccggaatt gcacctcatc atgccgaagc ttattgcgcc gcgttccgct atcaccgggc cactcgatag tttcaaggat atcaaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BrmeB.17377.c.B1.PS01927
PURIFICATION DATe: 10/31/2013
CONCENTRATION: 19mg/ml
OBSERVED MW: 39kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MHISRKKATI YDLSVLSGSS PSTVSAVLNG TWRKRRISQA TAEAILQLAD QHAYTANRQA QGLRHSRSGL VGLLLPLHDN RFFSAMAQIF EAHVRARSFC PVVVSASRDP EEERETAETL ISYAIDELFI CGATDPDGLH ELCEKAALPH VNIDLPGSRA PSVTTDNFAG GKMLTAAIIE YFGEEAPLQP EDLCLFGGRN DESTRQRICG FHQVKQTVLG ADPEGCVFKT GYSPANTLVA LNDYRARHGR LPRGLFVNSS INLEGLLRFM AEHPGEPLND IVIGCYDYDP FASFLPFPVI SILQDVEGMI AKAFDLMERR VTEPELHLIM PKLIAPRSAI TGPLDSFKDI K
Validated NT Sequence
tatttgatat ccttgaaact atcgagtggc ccggtgatag cggaacgcgg cgcaataagc ttcggcatga tgaggtgcaa ttccggttcc gtcacgcgcc gttccatcag gtcgaaggct tttgcaatca tgccttccac atcctgaagg atagaaataa ccggaaaggg caggaacgag gcgaagggat catagtcgta acagccaatg acaatatcat tgagcggctc gcccggatgc tctgccatga agcgtagcaa cccttccaga ttgatggagg aattgacgaa gagaccgcgc ggcagcctgc catgccgcgc gcgataatcg ttgagcgcaa ccagagtatt tgcaggcgaa tagccggtct tgaacacgca gccttccggg tcggccccca gcacggtctg cttcacctga tggaaaccgc aaatgcgctg gcgggtcgat tcatcgtttc gtccgccaaa aagacaaaga tcttcaggct gcaaaggcgc ctcttcgcca aaatattcga tgatggccgc agtgagcatc ttgccgcccg cgaaattatc cgtggtgacg gaaggagccc tggagccagg caggtcgata ttcacatgtg gcagggccgc tttttcgcag agctcatgca agccgtccgg atcagtggcg ccacaaataa acagctcgtc aatggcatag gaaatgaggg tttccgccgt ctcgcgctct tcttccgggt cgcggcttgc gctcaccacc acggggcaaa agctgcgcgc gcgtacatgt gcctcaaaaa tctgcgccat agcggagaaa aagcgattgt catgcaacgg gagcaaaagc cccaccaggc cggagcggga atgacgcagg ccttgtgcct gccggttggc ggtataggca tgctgatcgg ccagttgcaa tatggcctcc gccgtagcct gactgatacg ccgcttccgc catgtgccgt tcagcacggc gctcacagtc gaaggcgagc ttcccgacaa aacggaaaga tcatagatcg tcgccttttt cctgctgatg tgcatatggt ggtggtggtg
Expressed Protein Sequence
MAHHHHHHMH ISRKKATIYD LSVLSGSSPS TVSAVLNGTW RKRRISQATA EAILQLADQH AYTANRQAQG LRHSRSGLVG LLLPLHDNRF FSAMAQIFEA HVRARSFCPV VVSASRDPEE ERETAETLIS YAIDELFICG ATDPDGLHEL CEKAALPHVN IDLPGSRAPS VTTDNFAGGK MLTAAIIEYF GEEAPLQPED LCLFGGRNDE STRQRICGFH QVKQTVLGAD PEGCVFKTGY SPANTLVALN DYRARHGRLP RGLFVNSSIN LEGLLRFMAE HPGEPLNDIV IGCYDYDPFA SFLPFPVISI LQDVEGMIAK AFDLMERRVT EPELHLIMPK LIAPRSAITG PLDSFKDIK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCACA TCAGCAGGAA AAAGGCGACG ATCTATGATC TTTCCGTTTT GTCGGGAAGC TCGCCTTCGA CTGTGAGCGC CGTGCTGAAC GGCACATGGC GGAAGCGGCG TATCAGTCAG GCTACGGCGG AGGCCATATT GCAACTGGCC GATCAGCATG CCTATACCGC CAACCGGCAG GCACAAGGCC TGCGTCATTC CCGCTCCGGC CTGGTGGGGC TTTTGCTCCC GTTGCATGAC AATCGCTTTT TCTCCGCTAT GGCGCAGATT TTTGAGGCAC ATGTACGCGC GCGCAGCTTT TGCCCCGTGG TGGTGAGCGC AAGCCGCGAC CCGGAAGAAG AGCGCGAGAC GGCGGAAACC CTCATTTCCT ATGCCATTGA CGAGCTGTTT ATTTGTGGCG CCACTGATCC GGACGGCTTG CATGAGCTCT GCGAAAAAGC GGCCCTGCCA CATGTGAATA TCGACCTGCC TGGCTCCAGG GCTCCTTCCG TCACCACGGA TAATTTCGCG GGCGGCAAGA TGCTCACTGC GGCCATCATC GAATATTTTG GCGAAGAGGC GCCTTTGCAG CCTGAAGATC TTTGTCTTTT TGGCGGACGA AACGATGAAT CGACCCGCCA GCGCATTTGC GGTTTCCATC AGGTGAAGCA GACCGTGCTG GGGGCCGACC CGGAAGGCTG CGTGTTCAAG ACCGGCTATT CGCCTGCAAA TACTCTGGTT GCGCTCAACG ATTATCGCGC GCGGCATGGC AGGCTGCCGC GCGGTCTCTT CGTCAATTCC TCCATCAATC TGGAAGGGTT GCTACGCTTC ATGGCAGAGC ATCCGGGCGA GCCGCTCAAT GATATTGTCA TTGGCTGTTA CGACTATGAT CCCTTCGCCT CGTTCCTGCC CTTTCCGGTT ATTTCTATCC TTCAGGATGT GGAAGGCATG ATTGCAAAAG CCTTCGACCT GATGGAACGG CGCGTGACGG AACCGGAATT GCACCTCATC ATGCCGAAGC TTATTGCGCC GCGTTCCGCT ATCACCGGGC CACTCGATAG TTTCAAGGAT ATCAAATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T