SSGCID: Target Page

BrmeB.17377.b

Transcriptional regulator, LacI family

CENTER ID:	BrmeB.17377.b
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.17377.b.B1.GE36961	Full length( BrmeB.17377.b )		1	352

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.17377.b.B1.PW37378	Full length( BrmeB.17377.b )		1	352

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_2121
BV-BRC:	fig\|546272.3.peg.2121
RefSeq:	YP_002733713.1
UniProt:	C0RFT7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKGSVTVID IAREAGVSKS TVSLVLRDSP LVHAETRAKV QEAIEKLGYV YNRSAANLRQ AKSKIIGLVV NDLTNSFFAE LAVGVDRVMQ SAGYVQFLAN TAESIDRQRE VIASMREHGI AGLIVSPARG TEASDLKPLA KSGLPVVQMV RDVPGSGVSS IVSDNRGGVA KAVEHLVSLG HRAIAFMGGY ADIAVFSERL AGYRTGLEQA GIAFDEALVF TSAPSRAGGV EALEQMLRQG MKPTAAVCFN DAVAFGVCDG LRATHLEPGR DFGVVGFDDV IEAKTAVPAL TTVAVDPQGL GERAAQLLLK QINSERVEAE AQRLSVRLAV RASCGAPFRK PEEKFEWQSG AL

NT Sequence

ATGAGCAAGG GCAGCGTCAC CGTTATCGAC ATTGCGCGCG AGGCTGGCGT GTCGAAATCG ACCGTCTCTC TGGTGCTGCG TGACAGTCCG CTGGTCCATG CCGAAACCCG CGCCAAGGTG CAGGAGGCAA TCGAGAAACT GGGCTATGTC TATAACCGCT CGGCGGCCAA TCTCCGGCAG GCGAAATCGA AAATCATAGG CCTTGTGGTC AATGATCTGA CCAACAGTTT CTTTGCCGAA CTGGCGGTAG GTGTGGATCG CGTGATGCAG TCTGCGGGCT ATGTGCAGTT TCTGGCCAAT ACGGCGGAAA GCATTGATCG CCAGCGTGAA GTGATTGCCT CAATGCGCGA ACATGGCATT GCCGGGCTGA TCGTTTCGCC AGCGCGTGGA ACTGAGGCCA GTGATCTGAA ACCATTGGCG AAAAGCGGCC TTCCGGTTGT GCAGATGGTG CGCGATGTGC CGGGTTCGGG TGTTTCCTCC ATCGTTTCGG ATAATCGCGG CGGCGTGGCG AAGGCGGTCG AGCATCTGGT GTCGCTGGGG CATCGCGCCA TCGCTTTCAT GGGTGGTTAT GCGGATATAG CGGTGTTTTC CGAGCGTCTT GCCGGTTATC GCACAGGGTT GGAGCAAGCG GGCATTGCCT TCGACGAGGC GCTGGTTTTC ACCTCTGCGC CGTCACGCGC TGGTGGGGTT GAGGCGCTGG AACAGATGCT GCGGCAGGGC ATGAAACCGA CGGCGGCGGT GTGCTTCAAC GATGCGGTTG CTTTTGGTGT CTGCGATGGC TTGCGAGCCA CGCATCTGGA ACCGGGCCGG GATTTTGGCG TCGTCGGCTT TGACGATGTG ATCGAGGCAA AGACGGCTGT TCCGGCACTG ACGACCGTGG CTGTCGATCC GCAAGGTTTG GGAGAACGTG CGGCGCAGCT TTTGCTGAAA CAAATCAATT CGGAACGGGT GGAGGCGGAG GCGCAGCGTC TGTCGGTGCG TCTTGCAGTG CGTGCAAGTT GCGGCGCGCC GTTCAGAAAA CCGGAGGAGA AATTTGAATG GCAAAGTGGG GCCTTA

Details for BrmeB.17377.b.B1.GE36961

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	73

Validated AA Sequence

MQSAGYVQFL ANTAESIDRQ REVIASMREH GIAGLIVSPA RGTEASDLKP LAKSGLPVVQ MVRDVPGSGV SSIVSDNRGG VAKAVEHLVS LGHRAIAFMG GYADIAVFSE RLAGYRTGLE QAGIAFDEAL VFTSAPSRAG GVEALEQMLR QGMKPTAAVC FNDAVAFGVC DGLRATHLEP GRDFGVVGFD DVIEAKTAVP ALTTVAVDPQ GLGERAAQLL LKQINSERVE AEAQRLSVRL AVRASCGAPF RKPEEKFEWQ SGA

Validated NT Sequence

aggccccact ttgccattca aatttctcct ccggttttct gaacggcgcg ccgcaacttg cacgcactgc aagacgcacc gacagacgct gcgcctccgc ctccacccgt tccgaattga tttgtttcag caaaagctgc gccgcacgtt ctcccaaacc ttgcggatcg acagccacgg tcgtcagtgc cggaacagcc gtctttgcct cgatcacatc gtcaaagccg acgacgccaa aatcccggcc cggttccaga tgcgtggctc gcaagccatc gcagacacca aaagcaaccg catcgttgaa gcacaccgcc gccgtcggtt tcatgccctg ccgcagcatc tgttccagcg cctcaacccc accagcgcgt gacggcgcag aggtgaaaac cagcgcctcg tcgaaggcaa tgcccgcttg ctccaaccct gtgcgataac cggcaagacg ctcggaaaac accgctatat ccgcataacc acccatgaaa gcgatggcgc gatgccccag cgacaccaga tgctcgaccg ccttcgccac gccgccgcga ttatccgaaa cgatggagga aacacccgaa cccggcacat cgcgcaccat ctgcacaacc ggaaggccgc ttttcgccaa tggtttcaga tcactggcct cagttccacg cgctggcgaa acgatcagcc cggcaatgcc atgttcgcgc attgaggcaa tcacttcacg ctggcgatca atgctttccg ccgtattggc cagaaactgc acatagcccg cagactgcat cacgcgatcc acacctaccg ccagttcggc aaagaaactg ttggtcagat cattgaccac aaggcctatg attttcgatt tcgcctgccg gagattggcc gccgagcggt tatagacata gcccagtttc tcgattgcct cctgcacctt ggcgcgggtt tcggcatgga ccagcggact gtcacgcagc accagagaga cggtcgattt cgacacgcca gcctcgcgcg caatgtcgat aacggtgacg ctgcc

Expected Protein Sequence

MAHHHHHHMS KGSVTVIDIA REAGVSKSTV SLVLRDSPLV HAETRAKVQE AIEKLGYVYN RSAANLRQAK SKIIGLVVND LTNSFFAELA VGVDRVMQSA GYVQFLANTA ESIDRQREVI ASMREHGIAG LIVSPARGTE ASDLKPLAKS GLPVVQMVRD VPGSGVSSIV SDNRGGVAKA VEHLVSLGHR AIAFMGGYAD IAVFSERLAG YRTGLEQAGI AFDEALVFTS APSRAGGVEA LEQMLRQGMK PTAAVCFNDA VAFGVCDGLR ATHLEPGRDF GVVGFDDVIE AKTAVPALTT VAVDPQGLGE RAAQLLLKQI NSERVEAEAQ RLSVRLAVRA SCGAPFRKPE EKFEWQSGAL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca agggcagcgt caccgttatc gacattgcgc gcgaggctgg cgtgtcgaaa tcgaccgtct ctctggtgct gcgtgacagt ccgctggtcc atgccgaaac ccgcgccaag gtgcaggagg caatcgagaa actgggctat gtctataacc gctcggcggc caatctccgg caggcgaaat cgaaaatcat aggccttgtg gtcaatgatc tgaccaacag tttctttgcc gaactggcgg taggtgtgga tcgcgtgatg cagtctgcgg gctatgtgca gtttctggcc aatacggcgg aaagcattga tcgccagcgt gaagtgattg cctcaatgcg cgaacatggc attgccgggc tgatcgtttc gccagcgcgt ggaactgagg ccagtgatct gaaaccattg gcgaaaagcg gccttccggt tgtgcagatg gtgcgcgatg tgccgggttc gggtgtttcc tccatcgttt cggataatcg cggcggcgtg gcgaaggcgg tcgagcatct ggtgtcgctg gggcatcgcg ccatcgcttt catgggtggt tatgcggata tagcggtgtt ttccgagcgt cttgccggtt atcgcacagg gttggagcaa gcgggcattg ccttcgacga ggcgctggtt ttcacctctg cgccgtcacg cgctggtggg gttgaggcgc tggaacagat gctgcggcag ggcatgaaac cgacggcggc ggtgtgcttc aacgatgcgg ttgcttttgg tgtctgcgat ggcttgcgag ccacgcatct ggaaccgggc cgggattttg gcgtcgtcgg ctttgacgat gtgatcgagg caaagacggc tgttccggca ctgacgaccg tggctgtcga tccgcaaggt ttgggagaac gtgcggcgca gcttttgctg aaacaaatca attcggaacg ggtggaggcg gaggcgcagc gtctgtcggt gcgtcttgca gtgcgtgcaa gttgcggcgc gccgttcaga aaaccggagg agaaatttga atggcaaagt ggggccttat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BrmeB.17377.b.B1.PW37378

PURIFICATION DATe:	10/14/2013
CONCENTRATION:	24.4mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	12
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	73

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA AGGGCAGCGT CACCGTTATC GACATTGCGC GCGAGGCTGG CGTGTCGAAA TCGACCGTCT CTCTGGTGCT GCGTGACAGT CCGCTGGTCC ATGCCGAAAC CCGCGCCAAG GTGCAGGAGG CAATCGAGAA ACTGGGCTAT GTCTATAACC GCTCGGCGGC CAATCTCCGG CAGGCGAAAT CGAAAATCAT AGGCCTTGTG GTCAATGATC TGACCAACAG TTTCTTTGCC GAACTGGCGG TAGGTGTGGA TCGCGTGATG CAGTCTGCGG GCTATGTGCA GTTTCTGGCC AATACGGCGG AAAGCATTGA TCGCCAGCGT GAAGTGATTG CCTCAATGCG CGAACATGGC ATTGCCGGGC TGATCGTTTC GCCAGCGCGT GGAACTGAGG CCAGTGATCT GAAACCATTG GCGAAAAGCG GCCTTCCGGT TGTGCAGATG GTGCGCGATG TGCCGGGTTC GGGTGTTTCC TCCATCGTTT CGGATAATCG CGGCGGCGTG GCGAAGGCGG TCGAGCATCT GGTGTCGCTG GGGCATCGCG CCATCGCTTT CATGGGTGGT TATGCGGATA TAGCGGTGTT TTCCGAGCGT CTTGCCGGTT ATCGCACAGG GTTGGAGCAA GCGGGCATTG CCTTCGACGA GGCGCTGGTT TTCACCTCTG CGCCGTCACG CGCTGGTGGG GTTGAGGCGC TGGAACAGAT GCTGCGGCAG GGCATGAAAC CGACGGCGGC GGTGTGCTTC AACGATGCGG TTGCTTTTGG TGTCTGCGAT GGCTTGCGAG CCACGCATCT GGAACCGGGC CGGGATTTTG GCGTCGTCGG CTTTGACGAT GTGATCGAGG CAAAGACGGC TGTTCCGGCA CTGACGACCG TGGCTGTCGA TCCGCAAGGT TTGGGAGAAC GTGCGGCGCA GCTTTTGCTG AAACAAATCA ATTCGGAACG GGTGGAGGCG GAGGCGCAGC GTCTGTCGGT GCGTCTTGCA GTGCGTGCAA GTTGCGGCGC GCCGTTCAGA AAACCGGAGG AGAAATTTGA ATGGCAAAGT GGGGCCTTAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT