BrmeB.17377.a

Transcriptional regulator, LacI family

CENTER ID: BrmeB.17377.a
ORGANISM: Brucella melitensis ATCC 23457
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.17377.a.B1.GE36960 Full length( BrmeB.17377.a ) 1 340
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel14466_0222
BV-BRC: fig|546272.3.peg.222
RefSeq: YP_002731969.1
UniProt: C0RGQ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAATGKRATI HDVARLAGVS IKTVSRVYND EPNVREAIRE KVKQAGAQLR YRPNVAARNL VERRSHLIGL FFENPSSSYV TELQMGALER LRGTRYRLLI FPVENRADIR GALIETAHAF GLDGIIVTPP MSDDPDILQE LTASAMPFAR VAGDANVHPT DSVTIDDEAA AVDLMHYLLE LGHRKIAIVI GDPTHRSAEL RLEGYRRALR EAGIEGNSDY EVQGGFSFAS GLVAGRKLLS LPNRPTAIFA SNDDMAAAVM QVAYDAGIAV PDDLTVVGFD DSAIATMVSP QITTVRQPIF EMTRDAADML LRQMESGETS PAQRIDYKLI VRQSSARVPA
NT Sequence
ATGGCCGCGA CGGGCAAAAG AGCAACGATA CATGATGTGG CGCGCCTTGC TGGCGTGTCG ATCAAGACTG TTTCGCGCGT CTATAACGAC GAGCCGAACG TGCGCGAAGC CATACGCGAA AAAGTAAAAC AGGCCGGTGC GCAACTGCGC TATCGCCCCA ATGTGGCGGC ACGCAATCTG GTCGAGCGCC GCTCGCACCT GATCGGCCTT TTCTTTGAAA ACCCGAGCTC CAGTTATGTC ACCGAATTGC AGATGGGCGC ACTCGAACGC CTGCGTGGCA CGCGCTACCG GCTCCTGATC TTTCCGGTCG AAAACCGGGC CGATATTCGC GGTGCGCTGA TCGAAACCGC CCATGCTTTC GGTCTTGACG GCATCATCGT CACACCCCCC ATGTCGGATG ACCCGGACAT TTTGCAGGAA TTGACGGCCT CGGCCATGCC TTTCGCGCGT GTGGCGGGGG ATGCGAATGT GCATCCGACC GACAGCGTCA CCATCGACGA CGAGGCGGCG GCTGTCGATC TGATGCACTA TCTCCTGGAA CTCGGTCATC GAAAGATCGC CATCGTGATT GGCGATCCGA CACACCGTTC TGCCGAATTG CGCCTGGAAG GCTATCGCCG CGCGCTGCGT GAGGCGGGGA TCGAAGGCAA TTCCGATTAT GAGGTGCAGG GCGGGTTCAG TTTTGCAAGC GGCCTTGTGG CGGGCCGCAA GCTGCTTTCG CTCCCGAACA GGCCGACCGC CATCTTCGCC TCCAATGACG ATATGGCAGC CGCCGTGATG CAGGTCGCCT ATGATGCCGG GATCGCCGTT CCCGATGATC TTACGGTCGT CGGTTTTGAC GATAGTGCGA TTGCAACTAT GGTCTCGCCG CAGATCACCA CTGTTCGCCA GCCGATTTTC GAGATGACGC GGGATGCCGC CGATATGCTT TTGCGCCAGA TGGAGAGCGG GGAAACCTCG CCTGCACAGC GGATCGACTA TAAGCTCATC GTTCGCCAGT CTTCCGCCAG AGTTCCTGCC
Details for BrmeB.17377.a.B1.GE36960
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMA ATGKRATIHD VARLAGVSIK TVSRVYNDEP NVREAIREKV KQAGAQLRYR PNVAARNLVE RRSHLIGLFF ENPSSSYVTE LQMGALERLR GTRYRLLIFP VENRADIRGA LIETAHAFGL DGIIVTPPMS DDPDILQELT ASAMPFARVA GDANVHPTDS VTIDDEAAAV DLMHYLLELG HRKIAIVIGD PTHRSAELRL EGYRRALREA GIEGNSDYEV QGGFSFASGL VAGRKLLSLP NRPTAIFASN DDMAAAVMQV AYDAGIAVPD DLTVVGFDDS AIATMVSPQI TTVRQPIFEM TRDAADMLLR QMESGETSPA QRIDYKLIV
Validated NT Sequence
cgaacgatga gcttatagtc gatccgctgt gcaggcgagg tttccccgct ctccatctgg cgcaaaagca tatcggcggc atcccgcgtc atctcgaaaa tcggctggcg aacagtggtg atctgcggcg agaccatagt tgcaatcgca ctatcgtcaa aaccgacgac cgtaagatca tcgggaacgg cgatcccggc atcataggcg acctgcatca cggcggctgc catatcgtca ttggaggcga agatggcggt cggcctgttc gggagcgaaa gcagcttgcg gcccgccaca aggccgcttg caaaactgaa cccgccctgc acctcataat cggaattgcc ttcgatcccc gcctcacgca gcgcgcggcg atagccttcc aggcgcaatt cggcagaacg gtgtgtcgga tcgccaatca cgatggcgat ctttcgatga ccgagttcca ggagatagtg catcagatcg acagccgccg cctcgtcgtc gatggtgacg ctgtcggtcg gatgcacatt cgcatccccc gccacacgcg cgaaaggcat ggccgaggcc gtcaattcct gcaaaatgtc cgggtcatcc gacatggggg gtgtgacgat gatgccgtca agaccgaaag catgggcggt ttcgatcagc gcaccgcgaa tatcggcccg gttttcgacc ggaaagatca ggagccggta gcgcgtgcca cgcaggcgtt cgagtgcgcc catctgcaat tcggtgacat aactggagct cgggttttca aagaaaaggc cgatcaggtg cgagcggcgc tcgaccagat tgcgtgccgc cacattgggg cgatagcgca gttgcgcacc ggcctgtttt actttttcgc gtatggcttc gcgcacgttc ggctcgtcgt tatagacgcg cgaaacagtc ttgatcgaca cgccagcaag gcgcgccaca tcatgtatcg ttgctctttt gcccgtcgcg gccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMA ATGKRATIHD VARLAGVSIK TVSRVYNDEP NVREAIREKV KQAGAQLRYR PNVAARNLVE RRSHLIGLFF ENPSSSYVTE LQMGALERLR GTRYRLLIFP VENRADIRGA LIETAHAFGL DGIIVTPPMS DDPDILQELT ASAMPFARVA GDANVHPTDS VTIDDEAAAV DLMHYLLELG HRKIAIVIGD PTHRSAELRL EGYRRALREA GIEGNSDYEV QGGFSFASGL VAGRKLLSLP NRPTAIFASN DDMAAAVMQV AYDAGIAVPD DLTVVGFDDS AIATMVSPQI TTVRQPIFEM TRDAADMLLR QMESGETSPA QRIDYKLIVR QSSARVPA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccg cgacgggcaa aagagcaacg atacatgatg tggcgcgcct tgctggcgtg tcgatcaaga ctgtttcgcg cgtctataac gacgagccga acgtgcgcga agccatacgc gaaaaagtaa aacaggccgg tgcgcaactg cgctatcgcc ccaatgtggc ggcacgcaat ctggtcgagc gccgctcgca cctgatcggc cttttctttg aaaacccgag ctccagttat gtcaccgaat tgcagatggg cgcactcgaa cgcctgcgtg gcacgcgcta ccggctcctg atctttccgg tcgaaaaccg ggccgatatt cgcggtgcgc tgatcgaaac cgcccatgct ttcggtcttg acggcatcat cgtcacaccc cccatgtcgg atgacccgga cattttgcag gaattgacgg cctcggccat gcctttcgcg cgtgtggcgg gggatgcgaa tgtgcatccg accgacagcg tcaccatcga cgacgaggcg gcggctgtcg atctgatgca ctatctcctg gaactcggtc atcgaaagat cgccatcgtg attggcgatc cgacacaccg ttctgccgaa ttgcgcctgg aaggctatcg ccgcgcgctg cgtgaggcgg ggatcgaagg caattccgat tatgaggtgc agggcgggtt cagttttgca agcggccttg tggcgggccg caagctgctt tcgctcccga acaggccgac cgccatcttc gcctccaatg acgatatggc agccgccgtg atgcaggtcg cctatgatgc cgggatcgcc gttcccgatg atcttacggt cgtcggtttt gacgatagtg cgattgcaac tatggtctcg ccgcagatca ccactgttcg ccagccgatt ttcgagatga cgcgggatgc cgccgatatg cttttgcgcc agatggagag cggggaaacc tcgcctgcac agcggatcga ctataagctc atcgttcgcc agtcttccgc cagagttcct gcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat