SSGCID: Target Page

BrmeB.17368.b

High-affinity leucine-specific transport system, periplasmic binding protein LivK (TC 3.A.1.4.1)

CENTER ID:	BrmeB.17368.b
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.17368.b.B2.GE36958	Mature protein( BrmeB.17368.b )		28	376

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_1851
BV-BRC:	fig\|546272.3.peg.1851
RefSeq:	YP_002733462.1
UniProt:	C0RF36

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGAIEMKKSL FCGVCLCALV AMGGTSFADI MVGVGAPLTG SQAAFGEQIK RGVEAAVAEA NAKGGMNGEQ ITLVYGDDAA DPKQGISVAN KFVGDGVKFV IGHFNSGVSI PTSDIYAENG VLMIAPGTTN PTFTERELWN TFRTCRRDDK QGIVAGKYMA DNYKDGKVAI LHDKTPYGQG LADETKKSLN ENGMKETLYE GVNQGDKDFS ALISKMKAAG ITAVYWGGMH PEAGLLIRQM ADQGLKAQFI SGDGIVSNEL ASIAGDAVAG VMNTFGPDPR DDKANAELIK AFRDKGFEPE AYTFYAYAGV QSLVNAANAA GSNDPMEVAT AMKEKGPFKT VLGDISFDAK GDPSLSPYVM FEWRKGEDGK YNYFQK

NT Sequence

ATGGGAGCAA TCGAAATGAA GAAATCCTTA TTTTGCGGCG TTTGCCTTTG TGCGCTGGTC GCGATGGGCG GGACATCTTT CGCCGATATC ATGGTCGGTG TTGGTGCGCC CCTGACTGGC AGCCAGGCTG CCTTCGGCGA ACAGATCAAG CGCGGCGTGG AGGCGGCTGT CGCGGAAGCC AATGCCAAGG GCGGCATGAA TGGTGAGCAG ATCACCCTCG TTTATGGTGA TGACGCTGCC GATCCCAAGC AGGGCATCTC GGTTGCAAAC AAGTTCGTGG GCGATGGCGT CAAGTTTGTG ATTGGCCATT TCAATTCCGG TGTCAGCATT CCGACCTCGG ATATCTATGC CGAAAACGGC GTGCTGATGA TTGCACCGGG CACGACCAAC CCGACCTTTA CCGAGCGCGA ATTGTGGAAC ACATTCCGCA CCTGCCGCCG CGACGACAAG CAGGGCATTG TCGCAGGCAA GTACATGGCC GATAATTACA AGGACGGCAA GGTTGCCATT CTGCACGACA AGACGCCCTA TGGTCAGGGC CTTGCCGACG AAACTAAAAA GTCGCTCAAC GAGAACGGCA TGAAGGAAAC GCTCTATGAG GGCGTGAATC AGGGCGACAA GGATTTCTCC GCGCTCATTT CCAAGATGAA AGCTGCGGGC ATCACTGCCG TTTACTGGGG CGGTATGCAC CCGGAAGCTG GCCTTCTGAT TCGCCAGATG GCCGATCAGG GGCTGAAAGC CCAGTTCATT TCGGGTGACG GCATCGTGTC CAACGAGCTG GCGTCGATTG CCGGTGACGC CGTGGCCGGT GTGATGAATA CTTTCGGTCC CGATCCGCGC GACGACAAGG CCAATGCCGA ACTCATCAAG GCGTTCCGTG ACAAGGGTTT CGAGCCGGAA GCCTATACGT TCTATGCCTA TGCCGGTGTG CAATCGCTTG TGAACGCGGC CAATGCGGCT GGCAGCAATG ATCCGATGGA AGTCGCTACC GCGATGAAGG AAAAGGGTCC GTTCAAGACC GTGCTCGGCG ATATCTCCTT CGATGCGAAG GGTGATCCGA GCCTGTCGCC CTATGTCATG TTTGAATGGC GCAAGGGCGA AGACGGCAAG TATAATTACT TCCAGAAG

Details for BrmeB.17368.b.B2.GE36958

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA DIMVGVGAPL TGSQAAFGEQ IKRGVEAAVA EANAKGGMNG EQITLVYGDD AADPKQGISV ANKFVGDGVK FVIGHFNSGV SIPTSDIYAE NGVLMIAPGT TNPTFTEREL WNTFRTCRRD DKQGIVAGKY MADNYKDGKV AILHDKTPYG QGLADETKKS LNENGMKETL YEGVNQGDKD FSALISKMKA AGITAVYWGG MHPEAGLLIR QMADQGLKAQ FISGDGIVSN ELASIAGDAV AGVMNTFGPD PRDDKANAEL IKAFRDKGFE PEAYTFYAYA GVQSLVNAAN AAGSNDPMEV ATAMKEKGPF KTVLGDXSFD AKGDPSLSPY VMFEXRKGED GKYNYFQK

Validated NT Sequence

tcttactcnc ttacttctgg aagtaattat acttgccgtc ttcncccttg cgccnttcaa acatgacata gggcgacagg ctcggatcac ccttcgcatc gaagganata tcgccgagca cggtcttgaa cggacccttt tccttcatcg cggtagcgac ttccatcgga tcattgctgc cagccgcatt ggccgcgttc acaagcgatt gcacaccggc ataggcatag aacgtatagg cttccggctc gaaacccttg tcacggaacg ccttgatgag ttcggcattg gccttgtcgt cgcgcggatc gggaccgaaa gtattcatca caccggccac ggcgtcaccg gcaatcgacg ccagctcgtt ggacacgatg ccgtcacccg aaatgaactg ggctttcagc ccctgatcgg ccatctggcg aatcagaagg ccagcttccg ggtgcatacc gccccagtaa acggcagtga tgcccgcagc tttcatcttg gaaatgagcg cggagaaatc cttgtcgccc tgattcacgc cctcatagag cgtttccttc atgccgttct cgttgagcga ctttttagtt tcgtcggcaa ggccctgacc atagggcgtc ttgtcgtgca gaatggcaac cttgccgtcc ttgtaattat cggccatgta cttgcctgcg acaatgccct gcttgtcgtc gcggcggcag gtgcggaatg tgttccacaa ttcgcgctcg gtaaaggtcg ggttggtcgt gcccggtgca atcatcagca cgccgttttc ggcatagata tccgaggtcg gaatgctgac accggaattg aaatggccaa tcacaaactt gacgccatcg cccacgaact tgtttgcaac cgagatgccc tgcttgggat cggcagcgtc atcaccataa acgagggtga tctgctcacc attcatgccg cccttggcat tggcttccgc gacagccgcc tccacgccgc gcttgatctg ttcgccgaag gcagcctggc tgccagtcag gggcgcacca acaccgacca tgatatcggc catatggtgg tggtggtggt gagccatggt atntctcctt cttaa

Expected Protein Sequence

MAHHHHHHAD IMVGVGAPLT GSQAAFGEQI KRGVEAAVAE ANAKGGMNGE QITLVYGDDA ADPKQGISVA NKFVGDGVKF VIGHFNSGVS IPTSDIYAEN GVLMIAPGTT NPTFTERELW NTFRTCRRDD KQGIVAGKYM ADNYKDGKVA ILHDKTPYGQ GLADETKKSL NENGMKETLY EGVNQGDKDF SALISKMKAA GITAVYWGGM HPEAGLLIRQ MADQGLKAQF ISGDGIVSNE LASIAGDAVA GVMNTFGPDP RDDKANAELI KAFRDKGFEP EAYTFYAYAG VQSLVNAANA AGSNDPMEVA TAMKEKGPFK TVLGDISFDA KGDPSLSPYV MFEWRKGEDG KYNYFQK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgata tcatggtcgg tgttggtgcg cccctgactg gcagccaggc tgccttcggc gaacagatca agcgcggcgt ggaggcggct gtcgcggaag ccaatgccaa gggcggcatg aatggtgagc agatcaccct cgtttatggt gatgacgctg ccgatcccaa gcagggcatc tcggttgcaa acaagttcgt gggcgatggc gtcaagtttg tgattggcca tttcaattcc ggtgtcagca ttccgacctc ggatatctat gccgaaaacg gcgtgctgat gattgcaccg ggcacgacca acccgacctt taccgagcgc gaattgtgga acacattccg cacctgccgc cgcgacgaca agcagggcat tgtcgcaggc aagtacatgg ccgataatta caaggacggc aaggttgcca ttctgcacga caagacgccc tatggtcagg gccttgccga cgaaactaaa aagtcgctca acgagaacgg catgaaggaa acgctctatg agggcgtgaa tcagggcgac aaggatttct ccgcgctcat ttccaagatg aaagctgcgg gcatcactgc cgtttactgg ggcggtatgc acccggaagc tggccttctg attcgccaga tggccgatca ggggctgaaa gcccagttca tttcgggtga cggcatcgtg tccaacgagc tggcgtcgat tgccggtgac gccgtggccg gtgtgatgaa tactttcggt cccgatccgc gcgacgacaa ggccaatgcc gaactcatca aggcgttccg tgacaagggt ttcgagccgg aagcctatac gttctatgcc tatgccggtg tgcaatcgct tgtgaacgcg gccaatgcgg ctggcagcaa tgatccgatg gaagtcgcta ccgcgatgaa ggaaaagggt ccgttcaaga ccgtgctcgg cgatatctcc ttcgatgcga agggtgatcc gagcctgtcg ccctatgtca tgtttgaatg gcgcaagggc gaagacggca agtataatta cttccagaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat