SSGCID: Target Page

BrmeB.17368.a

ABC transporter, substrate-binding protein

CENTER ID:	BrmeB.17368.a
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.17368.a.B2.GE36957	Mature protein( BrmeB.17368.a )		23	368

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.17368.a.B2.PS01882	Mature protein( BrmeB.17368.a )		23	368

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_0016
BV-BRC:	fig\|546272.3.peg.16
RefSeq:	YP_002731793.1
UniProt:	C0RG81

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNLKLLSSVA FAATIGFASA AYADITIGVI APLTGPVAAF GDQVKKGAET AVEVINKAGG IKGEKVVLKF ADDAGEPKQG VSAANQIVGD GIKFVVGLVT TGVAVPVSEV LSENGVLMVT PTATGPDLTA RGLENVFRTC GRDGQQAEVM ADYVLKNMKD KKVAVIHDKG AYGKGLADAF KAAINKGGIT EVHYDSVTPG DKDFSALVTK LKSAGAEVVY FGGYHAEGGL LSRQLHDAGM QALVLGGEGL SNTEYWAIGG TNAQGTLFTN AKDATKNPAA KDAIQALKAK NIPAEAYTMN AYAAVEVIKA GIERAGSTDD SAAVAKALHD GKPIETAIGT LTYSETGDLS SPSFDIFKWD DGKIVGLE

NT Sequence

ATGAACCTGA AACTTCTGTC CAGCGTGGCT TTTGCCGCTA CCATCGGCTT TGCCAGCGCC GCTTACGCAG ACATCACCAT CGGTGTGATT GCACCGCTGA CGGGCCCGGT TGCCGCTTTC GGCGATCAGG TAAAAAAGGG CGCGGAAACC GCCGTGGAAG TCATCAACAA GGCTGGCGGC ATCAAGGGTG AGAAAGTCGT CCTGAAGTTT GCCGACGATG CGGGCGAGCC GAAGCAGGGC GTTTCCGCTG CCAACCAGAT CGTTGGCGAT GGCATCAAGT TTGTCGTCGG CCTGGTTACG ACCGGCGTTG CCGTCCCCGT TTCCGAGGTT TTGTCTGAAA ACGGCGTCCT CATGGTCACC CCGACGGCTA CCGGCCCGGA CCTGACCGCG CGCGGCCTGG AAAACGTGTT CCGTACCTGC GGCCGCGACG GCCAGCAGGC TGAAGTCATG GCTGATTACG TCCTGAAAAA CATGAAGGAC AAGAAGGTTG CCGTGATCCA CGACAAGGGC GCTTATGGCA AGGGTCTTGC CGATGCGTTC AAGGCGGCCA TCAACAAGGG CGGCATCACC GAAGTCCATT ATGATTCGGT CACACCGGGC GACAAGGATT TTTCCGCGCT CGTCACCAAG CTGAAGTCTG CCGGTGCTGA AGTGGTCTAT TTCGGCGGCT ACCATGCTGA AGGCGGTCTC CTGTCGCGCC AGCTCCACGA TGCTGGCATG CAGGCCCTGG TGCTCGGCGG CGAAGGCCTC TCCAACACCG AATATTGGGC AATCGGCGGC ACAAACGCAC AGGGTACGCT CTTCACCAAC GCCAAGGATG CCACGAAGAA CCCGGCTGCC AAGGACGCTA TCCAAGCCCT GAAGGCGAAG AACATCCCGG CTGAAGCTTA CACGATGAAC GCCTATGCGG CTGTGGAAGT CATCAAGGCC GGCATCGAAC GCGCCGGTTC GACTGACGAT TCCGCTGCCG TCGCCAAGGC GCTGCATGAC GGCAAGCCGA TCGAAACCGC AATCGGCACG CTGACCTATA GCGAAACCGG CGACCTTAGC TCGCCAAGCT TTGATATCTT CAAGTGGGAT GACGGCAAGA TCGTCGGTCT TGAA

Details for BrmeB.17368.a.B2.GE36957

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Validated AA Sequence

MADITIGVIA PLTGPVAAFG DQVKKGAETA VEVINKAGGI KGEKVVLKFA DDAGEPKQGV SAANQIVGDG IKFVVGLVTT GVAVPVSEVL SENGVLMVTP TATGPDLTAR GLENVFRTCG RDGQQAEVMA DYVLKNMKDK KVAVIHDKGA YGKGLADAFK AAINKGGITE VHYDSVTPGD KDFSALVTKL KSAGAEVVYF GGYHAEGGLL SRQLHDAGMQ ALVLGGEGLS NTEYWAIGGT NAQGTLFTNA KDATKNPAAK DAIQALKAKN IPAEAYTMNA YAAVEVIKAG IERAGSTDDS AAVAKALHDG KPIETAIGTL TYSETGDLSS PSFDIFKWD

Validated NT Sequence

tcatcccact tgaagatatc aaagcttggc gagctaaggt cgccggtttc gctataggtc agcgtgccga ttgcggtttc gatcggcttg ccgtcatgca gcgccttggc gacggcagcg gaatcgtcag tcgaaccggc gcgttcgatg ccggccttga tgacttccac agccgcatag gcgttcatcg tgtaagcttc agccgggatg ttcttcgcct tcagggcttg gatagcgtcc ttggcagccg ggttcttcgt ggcatccttg gcgttggtga agagcgtacc ctgtgcgttt gtgccgccga ttgcccaata ttcggtgttg gagaggcctt cgccgccgag caccagggcc tgcatgccag catcgtggag ctggcgcgac aggagaccgc cttcagcatg gtagccgccg aaatagacca cttcagcacc ggcagacttc agcttggtga cgagcgcgga aaaatccttg tcgcccggtg tgaccgaatc ataatggact tcggtgatgc cgcccttgtt gatggccgcc ttgaacgcat cggcaagacc cttgccataa gcgcccttgt cgtggatcac ggcaaccttc ttgtccttca tgtttttcag gacgtaatca gccatgactt cagcctgctg gccgtcgcgg ccgcaggtac ggaacacgtt ttccaggccg cgcgcggtca ggtccgggcc ggtagccgtc ggggtgacca tgaggacgcc gttttcagac aaaacctcgg aaacggggac ggcaacgccg gtcgtaacca ggccgacgac aaacttgatg ccatcgccaa cgatctggtt ggcagcggaa acgccctgct tcggctcgcc cgcatcgtcg gcaaacttca ggacgacttt ctcacccttg atgccgccag ccttgttgat gacttccacg gcggtttccg cgcccttttt tacctgatcg ccgaaagcgg caaccgggcc cgtcagcggt gcaatcacac cgatggtgat gtctgccata tg

Expected Protein Sequence

MAHHHHHHAD ITIGVIAPLT GPVAAFGDQV KKGAETAVEV INKAGGIKGE KVVLKFADDA GEPKQGVSAA NQIVGDGIKF VVGLVTTGVA VPVSEVLSEN GVLMVTPTAT GPDLTARGLE NVFRTCGRDG QQAEVMADYV LKNMKDKKVA VIHDKGAYGK GLADAFKAAI NKGGITEVHY DSVTPGDKDF SALVTKLKSA GAEVVYFGGY HAEGGLLSRQ LHDAGMQALV LGGEGLSNTE YWAIGGTNAQ GTLFTNAKDA TKNPAAKDAI QALKAKNIPA EAYTMNAYAA VEVIKAGIER AGSTDDSAAV AKALHDGKPI ETAIGTLTYS ETGDLSSPSF DIFKWDDGKI VGLE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcagaca tcaccatcgg tgtgattgca ccgctgacgg gcccggttgc cgctttcggc gatcaggtaa aaaagggcgc ggaaaccgcc gtggaagtca tcaacaaggc tggcggcatc aagggtgaga aagtcgtcct gaagtttgcc gacgatgcgg gcgagccgaa gcagggcgtt tccgctgcca accagatcgt tggcgatggc atcaagtttg tcgtcggcct ggttacgacc ggcgttgccg tccccgtttc cgaggttttg tctgaaaacg gcgtcctcat ggtcaccccg acggctaccg gcccggacct gaccgcgcgc ggcctggaaa acgtgttccg tacctgcggc cgcgacggcc agcaggctga agtcatggct gattacgtcc tgaaaaacat gaaggacaag aaggttgccg tgatccacga caagggcgct tatggcaagg gtcttgccga tgcgttcaag gcggccatca acaagggcgg catcaccgaa gtccattatg attcggtcac accgggcgac aaggattttt ccgcgctcgt caccaagctg aagtctgccg gtgctgaagt ggtctatttc ggcggctacc atgctgaagg cggtctcctg tcgcgccagc tccacgatgc tggcatgcag gccctggtgc tcggcggcga aggcctctcc aacaccgaat attgggcaat cggcggcaca aacgcacagg gtacgctctt caccaacgcc aaggatgcca cgaagaaccc ggctgccaag gacgctatcc aagccctgaa ggcgaagaac atcccggctg aagcttacac gatgaacgcc tatgcggctg tggaagtcat caaggccggc atcgaacgcg ccggttcgac tgacgattcc gctgccgtcg ccaaggcgct gcatgacggc aagccgatcg aaaccgcaat cggcacgctg acctatagcg aaaccggcga ccttagctcg ccaagctttg atatcttcaa gtgggatgac ggcaagatcg tcggtcttga atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BrmeB.17368.a.B2.PS01882

PURIFICATION DATe:	8/15/2013
CONCENTRATION:	26.9mg/ml
OBSERVED MW:	36kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCAGACA TCACCATCGG TGTGATTGCA CCGCTGACGG GCCCGGTTGC CGCTTTCGGC GATCAGGTAA AAAAGGGCGC GGAAACCGCC GTGGAAGTCA TCAACAAGGC TGGCGGCATC AAGGGTGAGA AAGTCGTCCT GAAGTTTGCC GACGATGCGG GCGAGCCGAA GCAGGGCGTT TCCGCTGCCA ACCAGATCGT TGGCGATGGC ATCAAGTTTG TCGTCGGCCT GGTTACGACC GGCGTTGCCG TCCCCGTTTC CGAGGTTTTG TCTGAAAACG GCGTCCTCAT GGTCACCCCG ACGGCTACCG GCCCGGACCT GACCGCGCGC GGCCTGGAAA ACGTGTTCCG TACCTGCGGC CGCGACGGCC AGCAGGCTGA AGTCATGGCT GATTACGTCC TGAAAAACAT GAAGGACAAG AAGGTTGCCG TGATCCACGA CAAGGGCGCT TATGGCAAGG GTCTTGCCGA TGCGTTCAAG GCGGCCATCA ACAAGGGCGG CATCACCGAA GTCCATTATG ATTCGGTCAC ACCGGGCGAC AAGGATTTTT CCGCGCTCGT CACCAAGCTG AAGTCTGCCG GTGCTGAAGT GGTCTATTTC GGCGGCTACC ATGCTGAAGG CGGTCTCCTG TCGCGCCAGC TCCACGATGC TGGCATGCAG GCCCTGGTGC TCGGCGGCGA AGGCCTCTCC AACACCGAAT ATTGGGCAAT CGGCGGCACA AACGCACAGG GTACGCTCTT CACCAACGCC AAGGATGCCA CGAAGAACCC GGCTGCCAAG GACGCTATCC AAGCCCTGAA GGCGAAGAAC ATCCCGGCTG AAGCTTACAC GATGAACGCC TATGCGGCTG TGGAAGTCAT CAAGGCCGGC ATCGAACGCG CCGGTTCGAC TGACGATTCC GCTGCCGTCG CCAAGGCGCT GCATGACGGC AAGCCGATCG AAACCGCAAT CGGCACGCTG ACCTATAGCG AAACCGGCGA CCTTAGCTCG CCAAGCTTTG ATATCTTCAA GTGGGATGAC GGCAAGATCG TCGGTCTTGA ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT