BrmeB.17356.a
ABC transporter, periplasmic substrate-binding protein
| CENTER ID: | BrmeB.17356.a |
| ORGANISM: | Brucella melitensis ATCC 23457 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeB.17356.a.B2.GE36956 | Mature protein( BrmeB.17356.a ) | 29 | 615 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeB.17356.a.B2.PS01888 | Mature protein( BrmeB.17356.a ) | 29 | 615 |
Structures
4ONY
DEPOSITED: 1/29/2014
DETERMINATION: XRay
CLONE: BrmeB.17356.a.B2.GE36956
PROTEIN: BrmeB.17356.a.B2.PS01888
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|546272.3.peg.2836 |
| RefSeq: | YP_002734333.1 |
| UniProt: | C0RL96 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLNRFIAFFR SVFLIGLVAT AFGALPARAA NETAPDYALS MHGDVALPAD YTHFPYTNPD APKKGSLTVG VVGTFDSLNP FVLKSMRTTA RGLYNDGEFG NMVYQTLMLR SRDEPFTLYS LLAEKVAIDP ERKWVEFTLN PKAKWSDGQP VTVDDVLFTY DILTEKGRPP YNSRMSRVAK IEKTGERSVR FTFNEKSDRE FPMLIAGSMP VLPKHAINRD TFGNSTLEPP IGSGPYVVAS VQPGQRIVYK RNPDYWGKDL PSQRGFNNFD KISIEYYRNE TSLFESFKKG ILDIFIEGNP IRWEKLYDFP AVEQGKVIKD TFEKGTPADM LGFVFNTRRP IFADRRVRQA LGLLFDFEWA NSNLFAGQYR RTQSFWEGSQ LSSVGRPADA RERELLAPFP GAVREDVMNG TWHPPVTDGS GHDRVPAKKA YDLLSQAGFQ FKDGMAIDPT AKPFAFEIMT RSPDEEKIAL AYQRNLSRLG IAVEIHTVDD AQYQQRLQTF DYDMILGALA SSLSPGNEQW LRWGSASRDV QGSFNFAGVA DPAVDAMIEA LLAARNRADF VSAVRALDRV LISGDYYVPL YHLPYQWVAR WDRIEHPQKT PLSGYQLPAW WHTSQ
NT Sequence
ATGTTGAACA GATTTATTGC TTTTTTCCGC AGTGTGTTTC TCATTGGCCT TGTTGCGACA GCCTTTGGCG CGTTGCCCGC GCGGGCGGCA AACGAGACAG CACCCGACTA TGCGCTCTCC ATGCATGGCG ACGTGGCTTT GCCTGCCGAT TATACCCATT TTCCCTACAC CAATCCCGAT GCCCCGAAAA AGGGTTCGCT GACCGTAGGC GTGGTGGGCA CTTTCGACAG CCTTAATCCC TTTGTGCTGA AAAGTATGCG CACCACCGCG CGCGGCCTGT ATAATGATGG CGAGTTTGGC AATATGGTGT ACCAGACGCT GATGCTGCGC TCGCGCGATG AGCCTTTTAC GCTCTATAGT CTGCTGGCTG AGAAAGTTGC CATCGATCCC GAGCGCAAAT GGGTGGAATT CACGCTCAAT CCAAAAGCCA AATGGTCAGA TGGCCAGCCT GTCACGGTGG ACGATGTTCT CTTCACCTAT GACATACTGA CCGAAAAGGG CCGCCCGCCC TATAACAGCC GCATGAGCCG GGTGGCGAAG ATCGAAAAGA CCGGCGAACG CTCAGTGCGC TTCACATTCA ACGAGAAATC CGACCGTGAG TTTCCGATGC TGATCGCAGG CTCGATGCCG GTTCTGCCGA AACATGCCAT CAACCGCGAT ACGTTCGGCA ATTCCACACT GGAACCGCCG ATCGGCAGCG GCCCCTATGT GGTCGCCAGT GTGCAGCCCG GCCAGCGGAT CGTCTACAAG CGCAACCCGG ACTATTGGGG CAAGGATCTG CCCTCGCAGC GCGGCTTCAA CAATTTCGAC AAGATCTCCA TCGAATATTA CCGCAACGAA ACCTCGCTGT TTGAATCCTT CAAAAAGGGC ATTCTCGACA TTTTCATTGA AGGCAATCCG ATACGCTGGG AAAAGCTCTA TGATTTTCCG GCGGTCGAGC AGGGCAAGGT CATCAAGGAC ACTTTCGAGA AGGGCACACC GGCCGATATG CTGGGCTTCG TGTTCAACAC GCGCCGCCCG ATCTTCGCTG ACCGGCGTGT GCGCCAGGCC CTTGGATTGC TGTTCGATTT TGAGTGGGCG AACAGCAATC TCTTTGCAGG GCAATATCGC CGTACGCAGA GCTTCTGGGA GGGGTCGCAA CTTTCATCCG TCGGCAGGCC CGCCGATGCG CGCGAAAGGG AGTTGCTGGC CCCCTTCCCC GGTGCTGTGC GCGAAGACGT GATGAATGGC ACCTGGCACC CGCCCGTTAC AGATGGCAGC GGCCATGACC GCGTTCCGGC CAAGAAGGCC TATGATCTTC TAAGCCAGGC CGGTTTCCAG TTCAAGGACG GCATGGCCAT AGATCCGACC GCTAAACCGT TTGCCTTCGA GATTATGACG CGATCACCGG ACGAGGAGAA AATCGCGCTC GCCTATCAGC GCAACCTCTC GCGCCTTGGC ATAGCAGTTG AAATCCACAC GGTGGACGAT GCGCAATATC AACAGCGCCT GCAAACCTTC GATTATGATA TGATCCTCGG TGCACTTGCC AGTTCGCTTT CTCCCGGCAA TGAGCAATGG TTGCGGTGGG GTTCCGCCTC GCGCGATGTG CAGGGCAGCT TCAACTTTGC GGGCGTGGCC GACCCTGCCG TTGATGCGAT GATCGAGGCG CTGCTTGCCG CGCGCAACCG CGCCGATTTC GTCAGCGCCG TGCGTGCACT CGACCGTGTG CTGATTTCAG GTGATTATTA TGTTCCGCTC TACCACCTGC CCTATCAATG GGTTGCGCGG TGGGACCGGA TTGAACATCC CCAAAAAACA CCACTTTCCG GCTACCAACT GCCGGCATGG TGGCACACGT CCCAA
Details for BrmeB.17356.a.B2.GE36956
| HARVESTED ON: | 5/21/2013 |
| SEQUENCED ON: | 5/23/2013 |
| EXPECTED MW: | 67kDa |
| OBSERVED MW: | 67kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MHGDVALPAD YTHFPYTNPD APKKGSLTVG VVGTFDSLNP FVLKSMRTTA RGLYNDGEFG NMVYQTLMLR SRDEPFTLYS LLAEKVAIDP ERKWVEFTLN PKAKWSDGQP VTVDDVLFTY DILTEKGRPP YNSRMSRVAK IEKTGERSVR FTFNEKSDRE FPMLIAGSMP VLPKHAINRD TFGNSTLEPP IGSGPYVVAS VQPGQRIVYK RNPDYXGKXL PSQRGFXNFD KISIEYYRNE TSLFESFKKG ILDIFIEGNP IRWEKLYDFP AVEQGKVIXD TFEKGTPADM LGFVFNTRRP IFADRRVRQA LGLLXDXEWA NSNLFAGQYR RTQSFWEXSQ LSSVGRPADA RERELLAPFP GAVREDVMNG TWHPPVTDGS GHDRVPAKKA YDLXSQAGFQ FKDGMAIDPT AKPFAXEIMT RSPDEEKIAL AYQRNLSRLG IAVEIHTVDD AQYQQRLQTF DYDMILGALA SSLSPGNEQW LRWGSASRDV QGSFNFAGVA DPAVDAMIEA LLAARNRADF VSAVRALDRV LISGDYYVPL YHLPYQWVAR WDRIEHPQKT PLSGYQLPAW WHTSQ
Validated NT Sequence
atcttactca cttattggga cgtgtgccac catgccggca gttggtagcc ggaaagtggt gttttttggg gatgttcaat ccggtcccac cgcgcaaccc attgataggg caggtggtag agcggaacat aataatcacc tgaaatcagc acacggtcga gtgcacgcac ggcgctgacg aaatcggcgc ggttgcgcgc ggcaagcagc gcctcgatca tcgcatcaac ggcagggtcg gccacgcccg caaagttgaa gctgccctgc acatcgcgcg aggcggaacc ccaccgcaac cattgctcat tgccgggaga aagcgaactg gcaagtgcac cgaggatcat atcataatcg aaggtttgca ggcgctgttg atattgcgca tcgtccaccg tgtggatttc aactgctatg ccaaggcgcg agaggttgcg ctgataggcg agcgcgattt tctcctcgtc cggtgatcgc gtcataatct cnaaggcaaa cggtttagcg gtcggatcta tggccatgcc gtccttgaac tggaaaccgg cctggcttan aagatcatag gccttcttgg ccggaacgcg gtcatggccg ctgccatctg taacgggcgg gtgccaggtg ccattcatca cgtcttcgcg cacagcaccg gggaangggg ccagcaactc cctttcgcgc gcatcggcgg gcctgccgac ggatgaaagt tgcgacnnct cccagaagct ctgcgtacgg cgatattgcc ctgcaaagag attgctgttc gcccactcna aatcnaacag caatccaagg gcctggcgca cacgccggtc agcgaagatc gggcggcgcg tgttgaacac gaagcccagc atatcggccg gtgtgccctt ctcgaaagtg tcnttgatga ccttgccctg ctcgaccgcc ggaaaatcat agagcttttc ccagcgtatn ggattgcctt caatgaaaat gtcgagaatg ccctttttga aggattcaaa cagcgaggtt tcgttgcggt aatattcgat ggagatcttg tcgaaattgt ngaagccgcg ctgcgagggc aganccttgc cncaatagtc cgggttgcgc ttgtagacga tccgctggcc gggctgcaca ctggcgacca cataggggcc gctgccgatc ggcggttcca gtgtggaatt gccgaacgta tcgcggttga tggcatgttt cggcagaacc ggcatcgagc ctgcgatcag catcggaaac tcacggtcgg atttctcgtt gaatgtgaag cgcactgagc gttcgccggt cttttcgatc ttcgccaccc ggctcatgcg gctgttatag ggcgggcggc ccttttcggt cagtatgtca taggtgaaga gaacatcgtc caccgtgaca ggctggccat ctgaccattt ggcttttgga ttgagcgtga attccaccca tttgcgctcg ggatcgatgg caactttctc agccagcaga ctatagagcg taaaaggctc atcgcgcgag cgcagcatca gcgtctggta caccatattg ccaaactcgc catcattata caggccgcgc gcggtggtgc gcatactttt cagcacaaag ggattaaggc tgtcgaaagt gcccaccacg cctacggtca gcgaaccctt tttcggggca tcgggattgg tgtagggaaa atgggtataa tcggcaggca aagccacgtc gccatgcatg gagagcgcat agtcgggtgc tgtctcgttt g
Expected Protein Sequence
MAHHHHHHAA NETAPDYALS MHGDVALPAD YTHFPYTNPD APKKGSLTVG VVGTFDSLNP FVLKSMRTTA RGLYNDGEFG NMVYQTLMLR SRDEPFTLYS LLAEKVAIDP ERKWVEFTLN PKAKWSDGQP VTVDDVLFTY DILTEKGRPP YNSRMSRVAK IEKTGERSVR FTFNEKSDRE FPMLIAGSMP VLPKHAINRD TFGNSTLEPP IGSGPYVVAS VQPGQRIVYK RNPDYWGKDL PSQRGFNNFD KISIEYYRNE TSLFESFKKG ILDIFIEGNP IRWEKLYDFP AVEQGKVIKD TFEKGTPADM LGFVFNTRRP IFADRRVRQA LGLLFDFEWA NSNLFAGQYR RTQSFWEGSQ LSSVGRPADA RERELLAPFP GAVREDVMNG TWHPPVTDGS GHDRVPAKKA YDLLSQAGFQ FKDGMAIDPT AKPFAFEIMT RSPDEEKIAL AYQRNLSRLG IAVEIHTVDD AQYQQRLQTF DYDMILGALA SSLSPGNEQW LRWGSASRDV QGSFNFAGVA DPAVDAMIEA LLAARNRADF VSAVRALDRV LISGDYYVPL YHLPYQWVAR WDRIEHPQKT PLSGYQLPAW WHTSQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcggcaa acgagacagc acccgactat gcgctctcca tgcatggcga cgtggctttg cctgccgatt atacccattt tccctacacc aatcccgatg ccccgaaaaa gggttcgctg accgtaggcg tggtgggcac tttcgacagc cttaatccct ttgtgctgaa aagtatgcgc accaccgcgc gcggcctgta taatgatggc gagtttggca atatggtgta ccagacgctg atgctgcgct cgcgcgatga gccttttacg ctctatagtc tgctggctga gaaagttgcc atcgatcccg agcgcaaatg ggtggaattc acgctcaatc caaaagccaa atggtcagat ggccagcctg tcacggtgga cgatgttctc ttcacctatg acatactgac cgaaaagggc cgcccgccct ataacagccg catgagccgg gtggcgaaga tcgaaaagac cggcgaacgc tcagtgcgct tcacattcaa cgagaaatcc gaccgtgagt ttccgatgct gatcgcaggc tcgatgccgg ttctgccgaa acatgccatc aaccgcgata cgttcggcaa ttccacactg gaaccgccga tcggcagcgg cccctatgtg gtcgccagtg tgcagcccgg ccagcggatc gtctacaagc gcaacccgga ctattggggc aaggatctgc cctcgcagcg cggcttcaac aatttcgaca agatctccat cgaatattac cgcaacgaaa cctcgctgtt tgaatccttc aaaaagggca ttctcgacat tttcattgaa ggcaatccga tacgctggga aaagctctat gattttccgg cggtcgagca gggcaaggtc atcaaggaca ctttcgagaa gggcacaccg gccgatatgc tgggcttcgt gttcaacacg cgccgcccga tcttcgctga ccggcgtgtg cgccaggccc ttggattgct gttcgatttt gagtgggcga acagcaatct ctttgcaggg caatatcgcc gtacgcagag cttctgggag gggtcgcaac tttcatccgt cggcaggccc gccgatgcgc gcgaaaggga gttgctggcc cccttccccg gtgctgtgcg cgaagacgtg atgaatggca cctggcaccc gcccgttaca gatggcagcg gccatgaccg cgttccggcc aagaaggcct atgatcttct aagccaggcc ggtttccagt tcaaggacgg catggccata gatccgaccg ctaaaccgtt tgccttcgag attatgacgc gatcaccgga cgaggagaaa atcgcgctcg cctatcagcg caacctctcg cgccttggca tagcagttga aatccacacg gtggacgatg cgcaatatca acagcgcctg caaaccttcg attatgatat gatcctcggt gcacttgcca gttcgctttc tcccggcaat gagcaatggt tgcggtgggg ttccgcctcg cgcgatgtgc agggcagctt caactttgcg ggcgtggccg accctgccgt tgatgcgatg atcgaggcgc tgcttgccgc gcgcaaccgc gccgatttcg tcagcgccgt gcgtgcactc gaccgtgtgc tgatttcagg tgattattat gttccgctct accacctgcc ctatcaatgg gttgcgcggt gggaccggat tgaacatccc caaaaaacac cactttccgg ctaccaactg ccggcatggt ggcacacgtc ccaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BrmeB.17356.a.B2.PS01888
| PURIFICATION DATe: | 8/15/2013 |
| CONCENTRATION: | 51.8mg/ml |
| OBSERVED MW: | 70kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MHGDVALPAD YTHFPYTNPD APKKGSLTVG VVGTFDSLNP FVLKSMRTTA RGLYNDGEFG NMVYQTLMLR SRDEPFTLYS LLAEKVAIDP ERKWVEFTLN PKAKWSDGQP VTVDDVLFTY DILTEKGRPP YNSRMSRVAK IEKTGERSVR FTFNEKSDRE FPMLIAGSMP VLPKHAINRD TFGNSTLEPP IGSGPYVVAS VQPGQRIVYK RNPDYXGKXL PSQRGFXNFD KISIEYYRNE TSLFESFKKG ILDIFIEGNP IRWEKLYDFP AVEQGKVIXD TFEKGTPADM LGFVFNTRRP IFADRRVRQA LGLLXDXEWA NSNLFAGQYR RTQSFWEXSQ LSSVGRPADA RERELLAPFP GAVREDVMNG TWHPPVTDGS GHDRVPAKKA YDLXSQAGFQ FKDGMAIDPT AKPFAXEIMT RSPDEEKIAL AYQRNLSRLG IAVEIHTVDD AQYQQRLQTF DYDMILGALA SSLSPGNEQW LRWGSASRDV QGSFNFAGVA DPAVDAMIEA LLAARNRADF VSAVRALDRV LISGDYYVPL YHLPYQWVAR WDRIEHPQKT PLSGYQLPAW WHTSQ
Validated NT Sequence
atcttactca cttattggga cgtgtgccac catgccggca gttggtagcc ggaaagtggt gttttttggg gatgttcaat ccggtcccac cgcgcaaccc attgataggg caggtggtag agcggaacat aataatcacc tgaaatcagc acacggtcga gtgcacgcac ggcgctgacg aaatcggcgc ggttgcgcgc ggcaagcagc gcctcgatca tcgcatcaac ggcagggtcg gccacgcccg caaagttgaa gctgccctgc acatcgcgcg aggcggaacc ccaccgcaac cattgctcat tgccgggaga aagcgaactg gcaagtgcac cgaggatcat atcataatcg aaggtttgca ggcgctgttg atattgcgca tcgtccaccg tgtggatttc aactgctatg ccaaggcgcg agaggttgcg ctgataggcg agcgcgattt tctcctcgtc cggtgatcgc gtcataatct cnaaggcaaa cggtttagcg gtcggatcta tggccatgcc gtccttgaac tggaaaccgg cctggcttan aagatcatag gccttcttgg ccggaacgcg gtcatggccg ctgccatctg taacgggcgg gtgccaggtg ccattcatca cgtcttcgcg cacagcaccg gggaangggg ccagcaactc cctttcgcgc gcatcggcgg gcctgccgac ggatgaaagt tgcgacnnct cccagaagct ctgcgtacgg cgatattgcc ctgcaaagag attgctgttc gcccactcna aatcnaacag caatccaagg gcctggcgca cacgccggtc agcgaagatc gggcggcgcg tgttgaacac gaagcccagc atatcggccg gtgtgccctt ctcgaaagtg tcnttgatga ccttgccctg ctcgaccgcc ggaaaatcat agagcttttc ccagcgtatn ggattgcctt caatgaaaat gtcgagaatg ccctttttga aggattcaaa cagcgaggtt tcgttgcggt aatattcgat ggagatcttg tcgaaattgt ngaagccgcg ctgcgagggc aganccttgc cncaatagtc cgggttgcgc ttgtagacga tccgctggcc gggctgcaca ctggcgacca cataggggcc gctgccgatc ggcggttcca gtgtggaatt gccgaacgta tcgcggttga tggcatgttt cggcagaacc ggcatcgagc ctgcgatcag catcggaaac tcacggtcgg atttctcgtt gaatgtgaag cgcactgagc gttcgccggt cttttcgatc ttcgccaccc ggctcatgcg gctgttatag ggcgggcggc ccttttcggt cagtatgtca taggtgaaga gaacatcgtc caccgtgaca ggctggccat ctgaccattt ggcttttgga ttgagcgtga attccaccca tttgcgctcg ggatcgatgg caactttctc agccagcaga ctatagagcg taaaaggctc atcgcgcgag cgcagcatca gcgtctggta caccatattg ccaaactcgc catcattata caggccgcgc gcggtggtgc gcatactttt cagcacaaag ggattaaggc tgtcgaaagt gcccaccacg cctacggtca gcgaaccctt tttcggggca tcgggattgg tgtagggaaa atgggtataa tcggcaggca aagccacgtc gccatgcatg gagagcgcat agtcgggtgc tgtctcgttt g
Expressed Protein Sequence
MAHHHHHHAA NETAPDYALS MHGDVALPAD YTHFPYTNPD APKKGSLTVG VVGTFDSLNP FVLKSMRTTA RGLYNDGEFG NMVYQTLMLR SRDEPFTLYS LLAEKVAIDP ERKWVEFTLN PKAKWSDGQP VTVDDVLFTY DILTEKGRPP YNSRMSRVAK IEKTGERSVR FTFNEKSDRE FPMLIAGSMP VLPKHAINRD TFGNSTLEPP IGSGPYVVAS VQPGQRIVYK RNPDYWGKDL PSQRGFNNFD KISIEYYRNE TSLFESFKKG ILDIFIEGNP IRWEKLYDFP AVEQGKVIKD TFEKGTPADM LGFVFNTRRP IFADRRVRQA LGLLFDFEWA NSNLFAGQYR RTQSFWEGSQ LSSVGRPADA RERELLAPFP GAVREDVMNG TWHPPVTDGS GHDRVPAKKA YDLLSQAGFQ FKDGMAIDPT AKPFAFEIMT RSPDEEKIAL AYQRNLSRLG IAVEIHTVDD AQYQQRLQTF DYDMILGALA SSLSPGNEQW LRWGSASRDV QGSFNFAGVA DPAVDAMIEA LLAARNRADF VSAVRALDRV LISGDYYVPL YHLPYQWVAR WDRIEHPQKT PLSGYQLPAW WHTSQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCGGCAA ACGAGACAGC ACCCGACTAT GCGCTCTCCA TGCATGGCGA CGTGGCTTTG CCTGCCGATT ATACCCATTT TCCCTACACC AATCCCGATG CCCCGAAAAA GGGTTCGCTG ACCGTAGGCG TGGTGGGCAC TTTCGACAGC CTTAATCCCT TTGTGCTGAA AAGTATGCGC ACCACCGCGC GCGGCCTGTA TAATGATGGC GAGTTTGGCA ATATGGTGTA CCAGACGCTG ATGCTGCGCT CGCGCGATGA GCCTTTTACG CTCTATAGTC TGCTGGCTGA GAAAGTTGCC ATCGATCCCG AGCGCAAATG GGTGGAATTC ACGCTCAATC CAAAAGCCAA ATGGTCAGAT GGCCAGCCTG TCACGGTGGA CGATGTTCTC TTCACCTATG ACATACTGAC CGAAAAGGGC CGCCCGCCCT ATAACAGCCG CATGAGCCGG GTGGCGAAGA TCGAAAAGAC CGGCGAACGC TCAGTGCGCT TCACATTCAA CGAGAAATCC GACCGTGAGT TTCCGATGCT GATCGCAGGC TCGATGCCGG TTCTGCCGAA ACATGCCATC AACCGCGATA CGTTCGGCAA TTCCACACTG GAACCGCCGA TCGGCAGCGG CCCCTATGTG GTCGCCAGTG TGCAGCCCGG CCAGCGGATC GTCTACAAGC GCAACCCGGA CTATTGGGGC AAGGATCTGC CCTCGCAGCG CGGCTTCAAC AATTTCGACA AGATCTCCAT CGAATATTAC CGCAACGAAA CCTCGCTGTT TGAATCCTTC AAAAAGGGCA TTCTCGACAT TTTCATTGAA GGCAATCCGA TACGCTGGGA AAAGCTCTAT GATTTTCCGG CGGTCGAGCA GGGCAAGGTC ATCAAGGACA CTTTCGAGAA GGGCACACCG GCCGATATGC TGGGCTTCGT GTTCAACACG CGCCGCCCGA TCTTCGCTGA CCGGCGTGTG CGCCAGGCCC TTGGATTGCT GTTCGATTTT GAGTGGGCGA ACAGCAATCT CTTTGCAGGG CAATATCGCC GTACGCAGAG CTTCTGGGAG GGGTCGCAAC TTTCATCCGT CGGCAGGCCC GCCGATGCGC GCGAAAGGGA GTTGCTGGCC CCCTTCCCCG GTGCTGTGCG CGAAGACGTG ATGAATGGCA CCTGGCACCC GCCCGTTACA GATGGCAGCG GCCATGACCG CGTTCCGGCC AAGAAGGCCT ATGATCTTCT AAGCCAGGCC GGTTTCCAGT TCAAGGACGG CATGGCCATA GATCCGACCG CTAAACCGTT TGCCTTCGAG ATTATGACGC GATCACCGGA CGAGGAGAAA ATCGCGCTCG CCTATCAGCG CAACCTCTCG CGCCTTGGCA TAGCAGTTGA AATCCACACG GTGGACGATG CGCAATATCA ACAGCGCCTG CAAACCTTCG ATTATGATAT GATCCTCGGT GCACTTGCCA GTTCGCTTTC TCCCGGCAAT GAGCAATGGT TGCGGTGGGG TTCCGCCTCG CGCGATGTGC AGGGCAGCTT CAACTTTGCG GGCGTGGCCG ACCCTGCCGT TGATGCGATG ATCGAGGCGC TGCTTGCCGC GCGCAACCGC GCCGATTTCG TCAGCGCCGT GCGTGCACTC GACCGTGTGC TGATTTCAGG TGATTATTAT GTTCCGCTCT ACCACCTGCC CTATCAATGG GTTGCGCGGT GGGACCGGAT TGAACATCCC CAAAAAACAC CACTTTCCGG CTACCAACTG CCGGCATGGT GGCACACGTC CCAATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT