BrmeB.17313.a

Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB

CENTER ID: BrmeB.17313.a
ORGANISM: Brucella melitensis ATCC 23457
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.17313.a.B1.GE36953 Full length( BrmeB.17313.a ) 1 404
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel14466_0194
BV-BRC: fig|546272.3.peg.194
RefSeq: YP_002731941.1
UniProt: C0RGM9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNKLFPPLQA KNAASTYPVM HADGETAFGI YVHWPFCLAK CPYCDFNSHV RHKPVDQARY GAAFRREMET LRERTGPRTV TSIFLGGGTP SLMQPETVGA LLDGIASYWS VAPDIEMTLE ANPTSVEADR FRGYRAAGVN RVSLGIQALN DPDLRRLGRM HSVAEAKAAI RLAREIFPRL SFDLIYARPD QTIEAWREEL KEAIGLAADH LSLYQLTIEE GTQFYNLWKA GKLTTPEPDL AAALYEETQR VTAEHGLPAY EISNHAVPGR ESQHNLVYWR YGQYAGIGPG AHGRFVENDV RTVTMTEKHP ETWLDHVERR GHGIIEEEYL DGGQEGDEFL MMGLRLREGI DLARYARLSG HAIDDKRLAK LIAEGMIEPM GGSLIRATPD GALVLDALVA DLAA
NT Sequence
ATGAACAAGC TGTTTCCCCC TTTGCAGGCC AAAAATGCTG CCAGCACCTA TCCCGTCATG CATGCTGATG GGGAAACCGC CTTTGGCATT TATGTGCACT GGCCGTTCTG TCTGGCCAAA TGCCCTTATT GCGATTTCAA CAGCCATGTC CGCCACAAGC CGGTGGATCA GGCGCGCTAT GGCGCGGCTT TCCGGCGCGA GATGGAAACC TTGCGTGAGC GCACAGGCCC GCGCACCGTC ACCAGCATTT TCCTGGGCGG CGGCACACCA TCGCTGATGC AGCCGGAAAC CGTGGGCGCT CTGCTGGATG GCATCGCATC GTATTGGTCT GTGGCGCCGG ACATTGAAAT GACGCTGGAA GCCAATCCGA CAAGCGTTGA GGCCGACCGT TTTCGCGGCT ATCGTGCGGC AGGCGTTAAC CGGGTGTCGC TTGGTATTCA GGCGTTGAAC GATCCCGATC TGCGCCGCCT TGGCCGGATG CATTCGGTTG CAGAGGCAAA GGCCGCGATC CGGCTGGCAC GCGAGATTTT CCCGCGCCTT TCCTTCGACC TTATCTATGC GCGGCCGGAC CAGACAATCG AGGCCTGGCG CGAGGAATTG AAGGAGGCGA TCGGCCTTGC TGCCGACCAT CTGTCGCTCT ACCAGCTTAC CATCGAGGAA GGCACGCAGT TCTATAATCT CTGGAAGGCG GGCAAGCTCA CCACGCCGGA GCCGGACCTT GCAGCCGCGC TCTATGAGGA AACGCAGCGT GTGACCGCCG AACATGGCCT TCCGGCCTAT GAGATTTCCA ACCATGCGGT GCCGGGGCGG GAATCGCAGC ACAATCTCGT CTATTGGCGT TATGGCCAAT ATGCTGGCAT CGGGCCCGGC GCACATGGCC GTTTCGTGGA AAATGACGTG CGCACCGTGA CCATGACTGA AAAGCATCCT GAAACATGGC TTGACCATGT GGAGCGGCGC GGCCACGGAA TTATCGAGGA AGAATATCTC GATGGCGGGC AGGAGGGCGA TGAATTCCTG ATGATGGGCC TGCGCCTGCG CGAAGGCATC GACCTTGCCC GTTACGCGCG GCTTTCCGGC CATGCGATCG ACGACAAACG GCTGGCAAAG CTGATCGCGG AAGGCATGAT CGAGCCGATG GGCGGCAGCC TGATCCGCGC AACGCCGGAT GGCGCGCTGG TGCTGGATGC ACTGGTTGCC GATCTGGCGG CA
Details for BrmeB.17313.a.B1.GE36953
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 46kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMN KLFPPLQAKN AASTYPVMHA DGETAFGIYV HWPFCLAKCP YCDFNSHVRH KPVDQARYGA AFRREMETLR ERTGPRTVTS IFLGGGTPSL MQPETVGALL DGIASYWSVA PDIEMTLEAN PTSVEADRFR GYRAAGVNRV SLGIQALNDP DLRRLGRMHS VAEAKAAIRL AREIFPRLSF DLIYARPDQT IEAWREELKE AIGLAADHLS LYQLTIEEGT QFYNLWKAGK LTTPXPXLAA ALYEETQRVT AEHGLPVYEI SNHAVPGRES QHNLVYWRYG QYAGIGPGAH XRFVENDVRT VTXTEKHPET WXDHVEXXAX ELSRXISRXR AXXXEXXXXG XXXKXSTXXX XXXXXXXRXX XXXXXXXXXX GXXXXXXXXX XXXXXX
Validated NT Sequence
tttntnnnnn ttaagaagga ganataccat ggctcaccac caccaccacc atatgaacaa gctgtttccc cctttgcagg ccaaaaatgc tgccagcacc tatcccgtca tgcatgctga tggggaaacc gcctttggca tttatgtgca ctggccgttc tgtctggcca aatgccctta ttgcgatttc aacagccatg tccgccacaa gccggtggat caggcgcgct atggcgcggc tttccggcgc gagatggaaa ccttgcgtga gcgcacaggc ccgcgcaccg tcaccagcat tttcctgggc ggcggcacac catcgctgat gcagccggaa accgtgggcg ctctgctgga tggcatcgca tcgtattggt ctgtggcgcc ggacattgaa atgacgctgg aagccaatcc gacaagcgtt gaggccgacc gttttcgcgg ctatcgtgcg gcaggcgtta accgggtgtc gcttggtatt caggcgttga acgatcccga tctgcgccgc cttggccgga tgcattcggt tgcagaggca aaggccgcga tccggctggc acgcgagatt ttcccgcgcc tttccttcga ccttatctat gcgcggccgg accagacaat cgaggcctgg cgcgaggaat tgaaggaggc gatcggcctt gctgccgacc atctgtcgct ctaccagctt accatcgagg aaggcacgca gttctataat ctctggaagg cgggcaagct caccacgccg ganccgganc ttgcagccgc gctctatgag gaaacgcagc gtgtgaccgc cgaacatggc cttccggtct atgagatttc caaccatgcg gtgccggggc gggaatcgca gcacaatctc gtctattggc gttatggcca atatgctggc atcgggcccg gcgcacatng ccgtttcgtg gaaaatgacg tgcgcaccgt gacnntgact gaaaagcatc ctgaaacatg gnttgaccat gtggagngnn cggccncnga attatcgaga nnaatatctc ganngcgggc ngnngnngan gaantccnga nganggggnn cnnnnngaag gnatcgaccn nnncnnntan nnncngnttn nnncnnnatc gacnnnnnan nnnggnnann nnnnnnnnna ngnacnnngg nnnnnnnnna ncnnnnnnnn nnngnnnnnn nnnnnnnnnn nntnnnna
Expected Protein Sequence
MAHHHHHHMN KLFPPLQAKN AASTYPVMHA DGETAFGIYV HWPFCLAKCP YCDFNSHVRH KPVDQARYGA AFRREMETLR ERTGPRTVTS IFLGGGTPSL MQPETVGALL DGIASYWSVA PDIEMTLEAN PTSVEADRFR GYRAAGVNRV SLGIQALNDP DLRRLGRMHS VAEAKAAIRL AREIFPRLSF DLIYARPDQT IEAWREELKE AIGLAADHLS LYQLTIEEGT QFYNLWKAGK LTTPEPDLAA ALYEETQRVT AEHGLPAYEI SNHAVPGRES QHNLVYWRYG QYAGIGPGAH GRFVENDVRT VTMTEKHPET WLDHVERRGH GIIEEEYLDG GQEGDEFLMM GLRLREGIDL ARYARLSGHA IDDKRLAKLI AEGMIEPMGG SLIRATPDGA LVLDALVADL AA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca agctgtttcc ccctttgcag gccaaaaatg ctgccagcac ctatcccgtc atgcatgctg atggggaaac cgcctttggc atttatgtgc actggccgtt ctgtctggcc aaatgccctt attgcgattt caacagccat gtccgccaca agccggtgga tcaggcgcgc tatggcgcgg ctttccggcg cgagatggaa accttgcgtg agcgcacagg cccgcgcacc gtcaccagca ttttcctggg cggcggcaca ccatcgctga tgcagccgga aaccgtgggc gctctgctgg atggcatcgc atcgtattgg tctgtggcgc cggacattga aatgacgctg gaagccaatc cgacaagcgt tgaggccgac cgttttcgcg gctatcgtgc ggcaggcgtt aaccgggtgt cgcttggtat tcaggcgttg aacgatcccg atctgcgccg ccttggccgg atgcattcgg ttgcagaggc aaaggccgcg atccggctgg cacgcgagat tttcccgcgc ctttccttcg accttatcta tgcgcggccg gaccagacaa tcgaggcctg gcgcgaggaa ttgaaggagg cgatcggcct tgctgccgac catctgtcgc tctaccagct taccatcgag gaaggcacgc agttctataa tctctggaag gcgggcaagc tcaccacgcc ggagccggac cttgcagccg cgctctatga ggaaacgcag cgtgtgaccg ccgaacatgg ccttccggcc tatgagattt ccaaccatgc ggtgccgggg cgggaatcgc agcacaatct cgtctattgg cgttatggcc aatatgctgg catcgggccc ggcgcacatg gccgtttcgt ggaaaatgac gtgcgcaccg tgaccatgac tgaaaagcat cctgaaacat ggcttgacca tgtggagcgg cgcggccacg gaattatcga ggaagaatat ctcgatggcg ggcaggaggg cgatgaattc ctgatgatgg gcctgcgcct gcgcgaaggc atcgaccttg cccgttacgc gcggctttcc ggccatgcga tcgacgacaa acggctggca aagctgatcg cggaaggcat gatcgagccg atgggcggca gcctgatccg cgcaacgccg gatggcgcgc tggtgctgga tgcactggtt gccgatctgg cggcatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat