BrmeB.01563.a
1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22)
| CENTER ID: | BrmeB.01563.a |
| ORGANISM: | Brucella melitensis ATCC 23457 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeB.01563.a.B1.GE36941 | Full length( BrmeB.01563.a ) | 1 | 320 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeB.01563.a.B1.PS01874 | Full length( BrmeB.01563.a ) | 1 | 320 | ||
| BrmeB.01563.a.B1.PS01875 | Full length( BrmeB.01563.a ) | 1 | 320 |
Structures
4MPQ
DEPOSITED: 9/13/2013
DETERMINATION: XRay
CLONE: BrmeB.01563.a.B1.GE36941
PROTEIN: BrmeB.01563.a.B1.PS01874
4O0K
DEPOSITED: 12/13/2013
DETERMINATION: XRay
CLONE: BrmeB.01563.a.B1.GE36941
PROTEIN: BrmeB.01563.a.B1.PS01875
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|546272.3.peg.2505 |
| RefSeq: | YP_002734061.1 |
| UniProt: | C0RKH4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKAEIFSGVI PALMTPCKPD RSPDFDALVR KGQELIGDGM SAVVYCGSMG DWPLLTDAQR MEGVERLVKA GIPVIVGTGA VNTASAVAHA AHAQKVGAKG LMVIPRVLSR GSAIAAQKAY FKAILSAAPD LPAVIYNSPY YGFATRADLF FDLRAEHPNL VGFKEFGGPA DMRYAAENIT SRDDGVSLMI GVDTAVFHGF VNCGATGAIT GIGNVLPKEV IHLCNLSQAA ALGDVDARQR AQELEQALAV LSSFDEGPDL VLYFKHMMVL KGDKEYTLHF NETDALSESQ RGYVETQFRL FNTWYAEWSK LPGAVQRCKA
NT Sequence
TTGAAAGCCG AGATTTTTTC CGGGGTGATC CCCGCTCTGA TGACACCGTG CAAGCCGGAC CGCAGCCCTG ATTTCGATGC GCTCGTGCGC AAGGGGCAGG AGCTGATTGG CGATGGTATG TCGGCGGTCG TCTATTGTGG TTCGATGGGC GATTGGCCGC TTCTGACGGA TGCGCAGCGC ATGGAGGGCG TGGAGCGTCT GGTAAAGGCC GGTATTCCCG TTATCGTCGG CACCGGCGCA GTCAATACGG CATCGGCTGT CGCTCATGCC GCACACGCGC AGAAAGTGGG CGCAAAGGGC CTTATGGTGA TCCCGCGCGT TCTGTCTCGC GGCTCGGCCA TTGCGGCGCA GAAGGCATAT TTCAAGGCAA TCCTTTCGGC GGCGCCGGAC CTGCCTGCGG TGATCTATAA CAGCCCCTAT TACGGCTTTG CCACGCGTGC CGATCTGTTT TTTGACCTGC GCGCCGAACA TCCAAATCTC GTTGGCTTCA AGGAATTCGG CGGCCCGGCG GACATGCGTT ATGCGGCGGA AAACATCACC AGCCGTGATG ATGGTGTTTC GCTGATGATC GGCGTCGATA CGGCCGTGTT CCATGGTTTT GTGAATTGCG GCGCAACGGG TGCGATTACC GGCATCGGCA ATGTGCTGCC AAAAGAAGTC ATTCACCTTT GCAATCTCTC GCAGGCAGCC GCCTTGGGCG ATGTCGATGC GCGCCAGCGC GCACAGGAAC TGGAACAGGC GCTTGCCGTC CTGTCCTCTT TCGATGAGGG ACCGGATCTG GTCCTCTACT TCAAGCACAT GATGGTGCTG AAGGGGGACA AGGAATATAC GCTGCATTTC AACGAGACCG ACGCATTGAG CGAAAGCCAG CGCGGCTATG TCGAAACGCA GTTCAGGCTT TTCAACACCT GGTATGCCGA GTGGAGCAAA CTGCCGGGTG CCGTACAGCG CTGCAAGGCG
Details for BrmeB.01563.a.B1.GE36941
| HARVESTED ON: | 5/21/2013 |
| SEQUENCED ON: | 5/23/2013 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHML KAEIFSGVIP ALMTPCKPDR SPDFDALVRK GQELIGDGMS AVVYCGSMGD WPLLTDAQRM EGVERLVKAG IPVIVGTGAV NTASAVAHAA HAQKVGAKGL MVIPRVLSRG SAIAAQKAYF KAILSAAPDL PAVIYNSPYY GFATRADLFF DLRAEHPNLV GFKEFGGPAD MRYAAENITS RDDGVSLMIG VDTAVFHGFV NCGATGAITG IGNVLPKEVI HLCNLSQAAA LGDVDARQRA QELEQALAVL SSFDEGPDLV LYFKHMMVLK GDKEYTLHFN ETDALSESQR GYVETQFRLF NTWYAEWSKL PGAVQRCKA
Validated NT Sequence
gancctatct tactcactta cgccttgcag cgctgtacgg cacccggcag tttgctccac tcggcatacc aggtgttgaa aagcctgaac tgcgtttcga catagccgcg ctggctttcg ctcaatgcgt cggtctcgtt gaaatgcagc gtatattcct tgtccccctt cagcaccatc atgtgcttga agtagaggac cagatccggt ccctcatcga aagaggacag gacggcaagc gcctgttcca gttcctgtgc gcgctggcgc gcatcgacat cgcccaaggc ggctgcctgc gagagattgc aaaggtgaat gacttctttt ggcagcacat tgccgatgcc ggtaatcgca cccgttgcgc cgcaattcac aaaaccatgg aacacggccg tatcgacgcc gatcatcagc gaaacaccat catcacggct ggtgatgttt tccgccgcat aacgcatgtc cgccgggccg ccgaattcct tgaagccaac gagatttgga tgttcggcgc gcaggtcaaa aaacagatcg gcacgcgtgg caaagccgta ataggggctg ttatagatca ccgcaggcag gtccggcgcc gccgaaagga ttgccttgaa atatgccttc tgcgccgcaa tggccgagcc gcgagacaga acgcgcggga tcaccataag gccctttgcg cccactttct gcgcgtgtgc ggcatgagcg acagccgatg ccgtattgac tgcgccggtg ccgacgataa cgggaatacc ggcctttacc agacgctcca cgccctccat gcgctgcgca tccgtcagaa gcggccaatc gcccatcgaa ccacaataga cgaccgccga cataccatcg ccaatcagct cctgcccctt gcgcacgagc gcatcgaaat cagggctgcg gtccggcttg cacggtgtca tcagagcggg gatcaccccg gaaaaaatct cggctttcaa catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMK AEIFSGVIPA LMTPCKPDRS PDFDALVRKG QELIGDGMSA VVYCGSMGDW PLLTDAQRME GVERLVKAGI PVIVGTGAVN TASAVAHAAH AQKVGAKGLM VIPRVLSRGS AIAAQKAYFK AILSAAPDLP AVIYNSPYYG FATRADLFFD LRAEHPNLVG FKEFGGPADM RYAAENITSR DDGVSLMIGV DTAVFHGFVN CGATGAITGI GNVLPKEVIH LCNLSQAAAL GDVDARQRAQ ELEQALAVLS SFDEGPDLVL YFKHMMVLKG DKEYTLHFNE TDALSESQRG YVETQFRLFN TWYAEWSKLP GAVQRCKA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgaaag ccgagatttt ttccggggtg atccccgctc tgatgacacc gtgcaagccg gaccgcagcc ctgatttcga tgcgctcgtg cgcaaggggc aggagctgat tggcgatggt atgtcggcgg tcgtctattg tggttcgatg ggcgattggc cgcttctgac ggatgcgcag cgcatggagg gcgtggagcg tctggtaaag gccggtattc ccgttatcgt cggcaccggc gcagtcaata cggcatcggc tgtcgctcat gccgcacacg cgcagaaagt gggcgcaaag ggccttatgg tgatcccgcg cgttctgtct cgcggctcgg ccattgcggc gcagaaggca tatttcaagg caatcctttc ggcggcgccg gacctgcctg cggtgatcta taacagcccc tattacggct ttgccacgcg tgccgatctg ttttttgacc tgcgcgccga acatccaaat ctcgttggct tcaaggaatt cggcggcccg gcggacatgc gttatgcggc ggaaaacatc accagccgtg atgatggtgt ttcgctgatg atcggcgtcg atacggccgt gttccatggt tttgtgaatt gcggcgcaac gggtgcgatt accggcatcg gcaatgtgct gccaaaagaa gtcattcacc tttgcaatct ctcgcaggca gccgccttgg gcgatgtcga tgcgcgccag cgcgcacagg aactggaaca ggcgcttgcc gtcctgtcct ctttcgatga gggaccggat ctggtcctct acttcaagca catgatggtg ctgaaggggg acaaggaata tacgctgcat ttcaacgaga ccgacgcatt gagcgaaagc cagcgcggct atgtcgaaac gcagttcagg cttttcaaca cctggtatgc cgagtggagc aaactgccgg gtgccgtaca gcgctgcaag gcgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BrmeB.01563.a.B1.PS01874
| PURIFICATION DATe: | 7/31/2013 |
| CONCENTRATION: | 77.1mg/ml |
| OBSERVED MW: | 34kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML KAEIFSGVIP ALMTPCKPDR SPDFDALVRK GQELIGDGMS AVVYCGSMGD WPLLTDAQRM EGVERLVKAG IPVIVGTGAV NTASAVAHAA HAQKVGAKGL MVIPRVLSRG SAIAAQKAYF KAILSAAPDL PAVIYNSPYY GFATRADLFF DLRAEHPNLV GFKEFGGPAD MRYAAENITS RDDGVSLMIG VDTAVFHGFV NCGATGAITG IGNVLPKEVI HLCNLSQAAA LGDVDARQRA QELEQALAVL SSFDEGPDLV LYFKHMMVLK GDKEYTLHFN ETDALSESQR GYVETQFRLF NTWYAEWSKL PGAVQRCKA
Validated NT Sequence
gancctatct tactcactta cgccttgcag cgctgtacgg cacccggcag tttgctccac tcggcatacc aggtgttgaa aagcctgaac tgcgtttcga catagccgcg ctggctttcg ctcaatgcgt cggtctcgtt gaaatgcagc gtatattcct tgtccccctt cagcaccatc atgtgcttga agtagaggac cagatccggt ccctcatcga aagaggacag gacggcaagc gcctgttcca gttcctgtgc gcgctggcgc gcatcgacat cgcccaaggc ggctgcctgc gagagattgc aaaggtgaat gacttctttt ggcagcacat tgccgatgcc ggtaatcgca cccgttgcgc cgcaattcac aaaaccatgg aacacggccg tatcgacgcc gatcatcagc gaaacaccat catcacggct ggtgatgttt tccgccgcat aacgcatgtc cgccgggccg ccgaattcct tgaagccaac gagatttgga tgttcggcgc gcaggtcaaa aaacagatcg gcacgcgtgg caaagccgta ataggggctg ttatagatca ccgcaggcag gtccggcgcc gccgaaagga ttgccttgaa atatgccttc tgcgccgcaa tggccgagcc gcgagacaga acgcgcggga tcaccataag gccctttgcg cccactttct gcgcgtgtgc ggcatgagcg acagccgatg ccgtattgac tgcgccggtg ccgacgataa cgggaatacc ggcctttacc agacgctcca cgccctccat gcgctgcgca tccgtcagaa gcggccaatc gcccatcgaa ccacaataga cgaccgccga cataccatcg ccaatcagct cctgcccctt gcgcacgagc gcatcgaaat cagggctgcg gtccggcttg cacggtgtca tcagagcggg gatcaccccg gaaaaaatct cggctttcaa catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMK AEIFSGVIPA LMTPCKPDRS PDFDALVRKG QELIGDGMSA VVYCGSMGDW PLLTDAQRME GVERLVKAGI PVIVGTGAVN TASAVAHAAH AQKVGAKGLM VIPRVLSRGS AIAAQKAYFK AILSAAPDLP AVIYNSPYYG FATRADLFFD LRAEHPNLVG FKEFGGPADM RYAAENITSR DDGVSLMIGV DTAVFHGFVN CGATGAITGI GNVLPKEVIH LCNLSQAAAL GDVDARQRAQ ELEQALAVLS SFDEGPDLVL YFKHMMVLKG DKEYTLHFNE TDALSESQRG YVETQFRLFN TWYAEWSKLP GAVQRCKA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGAAAG CCGAGATTTT TTCCGGGGTG ATCCCCGCTC TGATGACACC GTGCAAGCCG GACCGCAGCC CTGATTTCGA TGCGCTCGTG CGCAAGGGGC AGGAGCTGAT TGGCGATGGT ATGTCGGCGG TCGTCTATTG TGGTTCGATG GGCGATTGGC CGCTTCTGAC GGATGCGCAG CGCATGGAGG GCGTGGAGCG TCTGGTAAAG GCCGGTATTC CCGTTATCGT CGGCACCGGC GCAGTCAATA CGGCATCGGC TGTCGCTCAT GCCGCACACG CGCAGAAAGT GGGCGCAAAG GGCCTTATGG TGATCCCGCG CGTTCTGTCT CGCGGCTCGG CCATTGCGGC GCAGAAGGCA TATTTCAAGG CAATCCTTTC GGCGGCGCCG GACCTGCCTG CGGTGATCTA TAACAGCCCC TATTACGGCT TTGCCACGCG TGCCGATCTG TTTTTTGACC TGCGCGCCGA ACATCCAAAT CTCGTTGGCT TCAAGGAATT CGGCGGCCCG GCGGACATGC GTTATGCGGC GGAAAACATC ACCAGCCGTG ATGATGGTGT TTCGCTGATG ATCGGCGTCG ATACGGCCGT GTTCCATGGT TTTGTGAATT GCGGCGCAAC GGGTGCGATT ACCGGCATCG GCAATGTGCT GCCAAAAGAA GTCATTCACC TTTGCAATCT CTCGCAGGCA GCCGCCTTGG GCGATGTCGA TGCGCGCCAG CGCGCACAGG AACTGGAACA GGCGCTTGCC GTCCTGTCCT CTTTCGATGA GGGACCGGAT CTGGTCCTCT ACTTCAAGCA CATGATGGTG CTGAAGGGGG ACAAGGAATA TACGCTGCAT TTCAACGAGA CCGACGCATT GAGCGAAAGC CAGCGCGGCT ATGTCGAAAC GCAGTTCAGG CTTTTCAACA CCTGGTATGC CGAGTGGAGC AAACTGCCGG GTGCCGTACA GCGCTGCAAG GCGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for BrmeB.01563.a.B1.PS01875
| PURIFICATION DATe: | 7/31/2013 |
| CONCENTRATION: | 47mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML KAEIFSGVIP ALMTPCKPDR SPDFDALVRK GQELIGDGMS AVVYCGSMGD WPLLTDAQRM EGVERLVKAG IPVIVGTGAV NTASAVAHAA HAQKVGAKGL MVIPRVLSRG SAIAAQKAYF KAILSAAPDL PAVIYNSPYY GFATRADLFF DLRAEHPNLV GFKEFGGPAD MRYAAENITS RDDGVSLMIG VDTAVFHGFV NCGATGAITG IGNVLPKEVI HLCNLSQAAA LGDVDARQRA QELEQALAVL SSFDEGPDLV LYFKHMMVLK GDKEYTLHFN ETDALSESQR GYVETQFRLF NTWYAEWSKL PGAVQRCKA
Validated NT Sequence
gancctatct tactcactta cgccttgcag cgctgtacgg cacccggcag tttgctccac tcggcatacc aggtgttgaa aagcctgaac tgcgtttcga catagccgcg ctggctttcg ctcaatgcgt cggtctcgtt gaaatgcagc gtatattcct tgtccccctt cagcaccatc atgtgcttga agtagaggac cagatccggt ccctcatcga aagaggacag gacggcaagc gcctgttcca gttcctgtgc gcgctggcgc gcatcgacat cgcccaaggc ggctgcctgc gagagattgc aaaggtgaat gacttctttt ggcagcacat tgccgatgcc ggtaatcgca cccgttgcgc cgcaattcac aaaaccatgg aacacggccg tatcgacgcc gatcatcagc gaaacaccat catcacggct ggtgatgttt tccgccgcat aacgcatgtc cgccgggccg ccgaattcct tgaagccaac gagatttgga tgttcggcgc gcaggtcaaa aaacagatcg gcacgcgtgg caaagccgta ataggggctg ttatagatca ccgcaggcag gtccggcgcc gccgaaagga ttgccttgaa atatgccttc tgcgccgcaa tggccgagcc gcgagacaga acgcgcggga tcaccataag gccctttgcg cccactttct gcgcgtgtgc ggcatgagcg acagccgatg ccgtattgac tgcgccggtg ccgacgataa cgggaatacc ggcctttacc agacgctcca cgccctccat gcgctgcgca tccgtcagaa gcggccaatc gcccatcgaa ccacaataga cgaccgccga cataccatcg ccaatcagct cctgcccctt gcgcacgagc gcatcgaaat cagggctgcg gtccggcttg cacggtgtca tcagagcggg gatcaccccg gaaaaaatct cggctttcaa catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMK AEIFSGVIPA LMTPCKPDRS PDFDALVRKG QELIGDGMSA VVYCGSMGDW PLLTDAQRME GVERLVKAGI PVIVGTGAVN TASAVAHAAH AQKVGAKGLM VIPRVLSRGS AIAAQKAYFK AILSAAPDLP AVIYNSPYYG FATRADLFFD LRAEHPNLVG FKEFGGPADM RYAAENITSR DDGVSLMIGV DTAVFHGFVN CGATGAITGI GNVLPKEVIH LCNLSQAAAL GDVDARQRAQ ELEQALAVLS SFDEGPDLVL YFKHMMVLKG DKEYTLHFNE TDALSESQRG YVETQFRLFN TWYAEWSKLP GAVQRCKA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGAAAG CCGAGATTTT TTCCGGGGTG ATCCCCGCTC TGATGACACC GTGCAAGCCG GACCGCAGCC CTGATTTCGA TGCGCTCGTG CGCAAGGGGC AGGAGCTGAT TGGCGATGGT ATGTCGGCGG TCGTCTATTG TGGTTCGATG GGCGATTGGC CGCTTCTGAC GGATGCGCAG CGCATGGAGG GCGTGGAGCG TCTGGTAAAG GCCGGTATTC CCGTTATCGT CGGCACCGGC GCAGTCAATA CGGCATCGGC TGTCGCTCAT GCCGCACACG CGCAGAAAGT GGGCGCAAAG GGCCTTATGG TGATCCCGCG CGTTCTGTCT CGCGGCTCGG CCATTGCGGC GCAGAAGGCA TATTTCAAGG CAATCCTTTC GGCGGCGCCG GACCTGCCTG CGGTGATCTA TAACAGCCCC TATTACGGCT TTGCCACGCG TGCCGATCTG TTTTTTGACC TGCGCGCCGA ACATCCAAAT CTCGTTGGCT TCAAGGAATT CGGCGGCCCG GCGGACATGC GTTATGCGGC GGAAAACATC ACCAGCCGTG ATGATGGTGT TTCGCTGATG ATCGGCGTCG ATACGGCCGT GTTCCATGGT TTTGTGAATT GCGGCGCAAC GGGTGCGATT ACCGGCATCG GCAATGTGCT GCCAAAAGAA GTCATTCACC TTTGCAATCT CTCGCAGGCA GCCGCCTTGG GCGATGTCGA TGCGCGCCAG CGCGCACAGG AACTGGAACA GGCGCTTGCC GTCCTGTCCT CTTTCGATGA GGGACCGGAT CTGGTCCTCT ACTTCAAGCA CATGATGGTG CTGAAGGGGG ACAAGGAATA TACGCTGCAT TTCAACGAGA CCGACGCATT GAGCGAAAGC CAGCGCGGCT ATGTCGAAAC GCAGTTCAGG CTTTTCAACA CCTGGTATGC CGAGTGGAGC AAACTGCCGG GTGCCGTACA GCGCTGCAAG GCGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT