SSGCID: Target Page

BrmeB.01365.d

Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

CENTER ID:	BrmeB.01365.d
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.01365.d.B1.GE36939	Full length( BrmeB.01365.d )		1	257

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_3002
BV-BRC:	fig\|546272.3.peg.3002
RefSeq:	YP_002734488.1
UniProt:	C0RLQ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDHSGEFAGR SVLVTGAGGG LGRALAALFA ARGARVIGCD VSMDLMAASD FASRHVFDLL DRAELAAAAN KLILADGVPD IVINNAGWTR AETFEGLEQD RIEVEIDLNL TGVASFSNIM AQAMATRGHG AFVFVSSVNS LQHFGNPAYA AAKAGINAFA RGIAVEFGQR GVRANVVCPG SIRTPAWDHR IAQDPSVLDR LQRLYPLGRI VNVGEVAEAV AFLASERASG ITGAVLPVDA GLTAGCRPFI EDILGGN

NT Sequence

GTGGATCATT CAGGTGAATT TGCCGGAAGA TCGGTTCTTG TAACCGGTGC TGGCGGCGGC TTGGGCAGGG CTCTTGCGGC GCTGTTTGCC GCGCGCGGCG CGCGCGTCAT CGGCTGTGAT GTATCGATGG ATCTGATGGC CGCTTCGGAT TTCGCCAGCC GCCATGTATT CGACCTGCTT GACCGTGCGG AGCTTGCGGC GGCTGCCAAC AAGCTCATTC TTGCCGACGG CGTGCCGGAT ATCGTCATCA ACAATGCGGG CTGGACCCGC GCCGAGACAT TCGAGGGCTT GGAACAGGAC CGGATCGAGG TGGAAATCGA CCTTAACCTG ACCGGCGTGG CGAGCTTTTC CAATATCATG GCGCAGGCCA TGGCCACGCG CGGCCATGGC GCTTTTGTTT TCGTTTCGTC CGTCAACTCG CTCCAGCATT TCGGCAACCC GGCCTATGCG GCGGCCAAGG CAGGCATCAA TGCTTTTGCG CGCGGCATTG CGGTGGAATT CGGCCAGCGG GGTGTGCGCG CCAATGTGGT TTGCCCCGGT TCCATTCGCA CCCCCGCATG GGATCACCGC ATCGCACAGG ATCCGTCCGT GCTGGACCGT TTGCAGCGGC TTTATCCGCT GGGGCGCATC GTTAATGTTG GCGAGGTGGC CGAGGCGGTA GCATTTCTGG CGTCCGAGCG GGCATCGGGC ATCACCGGCG CGGTTTTGCC GGTGGATGCG GGCCTGACGG CGGGGTGCCG TCCGTTCATC GAAGATATTC TGGGAGGGAA T

Details for BrmeB.01365.d.B1.GE36939

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	28kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV DHSGEFAGRS VLVTGAGGGL GRALAALFAA RGARVIGCDV SMDLMAASDF ASRHVFDLLD RAELAAAANK LILADGVPDI VINNAGWTRA ETFEGLEQDR IEVEIDLNLT GVASFSNIMA QAMATRGHGA FVFVSSVNSL QHFGNPAYAA AKAGINAFAR GIAVEFGQRG VRANVVCPGS IRTPAWDHRI AQDPSVLDRL QRLYPLGRIV NVGEVAEAVA FLASERASGI TGAVLPVDAG LTAGCRPFIE DILGGN

Validated NT Sequence

tcttactcac ttaattccct cccagaatat cttcgatgaa cggacggcac cccgccgtca ggcccgcatc caccggcaaa accgcgccgg tgatgcccga tgcccgctcg gacgccagaa atgctaccgc ctcggccacc tcgccaacat taacgatgcg ccccagcgga taaagccgct gcaaacggtc cagcacggac ggatcctgtg cgatgcggtg atcccatgcg ggggtgcgaa tggaaccggg gcaaaccaca ttggcgcgca caccccgctg gccgaattcc accgcaatgc cgcgcgcaaa agcattgatg cctgccttgg ccgccgcata ggccgggttg ccgaaatgct ggagcgagtt gacggacgaa acgaaaacaa aagcgccatg gccgcgcgtg gccatggcct gcgccatgat attggaaaag ctcgccacgc cggtcaggtt aaggtcgatt tccacctcga tccggtcctg ttccaagccc tcgaatgtct cggcgcgggt ccagcccgca ttgttgatga cgatatccgg cacgccgtcg gcaagaatga gcttgttggc agccgccgca agctccgcac ggtcaagcag gtcgaataca tggcggctgg cgaaatccga agcggccatc agatccatcg atacatcaca gccgatgacg cgcgcgccgc gcgcggcaaa cagcgccgca agagccctgc ccaagccgcc gccagcaccg gttacaagaa ccgatcttcc ggcaaattca cctgaatgat ccaccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHMD HSGEFAGRSV LVTGAGGGLG RALAALFAAR GARVIGCDVS MDLMAASDFA SRHVFDLLDR AELAAAANKL ILADGVPDIV INNAGWTRAE TFEGLEQDRI EVEIDLNLTG VASFSNIMAQ AMATRGHGAF VFVSSVNSLQ HFGNPAYAAA KAGINAFARG IAVEFGQRGV RANVVCPGSI RTPAWDHRIA QDPSVLDRLQ RLYPLGRIVN VGEVAEAVAF LASERASGIT GAVLPVDAGL TAGCRPFIED ILGGN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggatc attcaggtga atttgccgga agatcggttc ttgtaaccgg tgctggcggc ggcttgggca gggctcttgc ggcgctgttt gccgcgcgcg gcgcgcgcgt catcggctgt gatgtatcga tggatctgat ggccgcttcg gatttcgcca gccgccatgt attcgacctg cttgaccgtg cggagcttgc ggcggctgcc aacaagctca ttcttgccga cggcgtgccg gatatcgtca tcaacaatgc gggctggacc cgcgccgaga cattcgaggg cttggaacag gaccggatcg aggtggaaat cgaccttaac ctgaccggcg tggcgagctt ttccaatatc atggcgcagg ccatggccac gcgcggccat ggcgcttttg ttttcgtttc gtccgtcaac tcgctccagc atttcggcaa cccggcctat gcggcggcca aggcaggcat caatgctttt gcgcgcggca ttgcggtgga attcggccag cggggtgtgc gcgccaatgt ggtttgcccc ggttccattc gcacccccgc atgggatcac cgcatcgcac aggatccgtc cgtgctggac cgtttgcagc ggctttatcc gctggggcgc atcgttaatg ttggcgaggt ggccgaggcg gtagcatttc tggcgtccga gcgggcatcg ggcatcaccg gcgcggtttt gccggtggat gcgggcctga cggcggggtg ccgtccgttc atcgaagata ttctgggagg gaattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat