SSGCID: Target Page

BrmeB.01365.c

Ribitol 2-dehydrogenase (EC 1.1.1.56)

CENTER ID:	BrmeB.01365.c
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.01365.c.B1.GE36938	Full length( BrmeB.01365.c )		1	216

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_2533
BV-BRC:	fig\|546272.3.peg.2533
RefSeq:	YP_002734088.1
UniProt:	C0RKK1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIGAGITVFL VDRDENALAE KCVELGDKAK PLVVDLLDPA SVATMMPQIL EQAGQLDIFH ANAGSYIGGP LWEGDPDAWD RMLNLNINAA FRTVHAVLPH MMERKTGDII MTSSVAGVVP VIWEPIYTAS KHAVQAFVHT VRRQVAPHGI RVGGVLPGPV VTALISDWPQ AKLDEALAAG SLMEPKEVAE SVMFMLTRPR NITIRDLVIL PQSLDL

NT Sequence

ATGATTGGGG CGGGCATAAC CGTGTTTCTT GTCGACCGCG ACGAGAACGC GCTTGCGGAA AAGTGCGTTG AGCTTGGCGA CAAGGCCAAA CCGCTGGTCG TCGATCTGCT CGATCCGGCA TCCGTCGCCA CCATGATGCC GCAGATTCTT GAACAGGCAG GCCAGCTCGA TATTTTCCAC GCCAATGCCG GCTCCTATAT TGGCGGGCCG CTCTGGGAGG GCGATCCGGA TGCATGGGAT CGCATGTTGA ACCTCAATAT TAATGCGGCT TTCCGTACCG TCCATGCCGT TCTGCCGCAT ATGATGGAAC GCAAGACTGG CGACATCATC ATGACCAGCT CTGTTGCTGG CGTCGTGCCG GTGATATGGG AACCGATTTA TACGGCGTCC AAACATGCCG TGCAGGCTTT CGTGCACACG GTTCGCCGTC AGGTCGCCCC GCATGGTATT CGCGTTGGCG GTGTTCTGCC GGGCCCGGTC GTCACTGCGC TCATCAGCGA CTGGCCGCAA GCCAAGCTCG ATGAGGCGCT GGCGGCAGGC AGCCTGATGG AGCCGAAAGA GGTTGCGGAA TCCGTGATGT TCATGCTGAC ACGCCCGCGC AATATCACCA TCCGCGATCT GGTTATTCTT CCGCAAAGTC TCGATCTC

Details for BrmeB.01365.c.B1.GE36938

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	24kDa
OBSERVED MW:	22kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI GAGITVFLVD RDENALAEKC VELGDKAKPL VVDLLDPASV ATMMPQILEQ AGQLDIFHAN AGSYIGGPLW EGDPDAWDRM LNLNINAAFR TVHAVLPHMM ERKTGDIIMT SSVAGVVPVI WEPIYTASKH AVQAFVHTVR RQVAPHGIRV GGVLPGPVVT ALISDWPQAK LDEALAAGSL MEPKEVAESV MFMLTRPRNI TIRDLVILPQ SLDL

Validated NT Sequence

anantttgtn nnntttaaga aggaganata ccatggctca ccaccaccac caccatatga ttggggcggg cataaccgtg tttcttgtcg accgcgacga gaacgcgctt gcggaaaagt gcgttgagct tggcgacaag gccaaaccgc tggtcgtcga tctgctcgat ccggcatccg tcgccaccat gatgccgcag attcttgaac aggcaggcca gctcgatatt ttccacgcca atgccggctc ctatattggc gggccgctct gggagggcga tccggatgca tgggatcgca tgttgaacct caatattaat gcggctttcc gtaccgtcca tgccgttctg ccgcatatga tggaacgcaa gactggcgac atcatcatga ccagctctgt tgctggcgtc gtgccggtga tatgggaacc gatttatacg gcgtccaaac atgccgtgca ggctttcgtg cacacggttc gccgtcaggt cgccccgcat ggtattcgcg ttggcggtgt tctgccgggc ccggtcgtca ctgcgctcat cagcgactgg ccgcaagcca agctcgatga ggcgctggcg gcaggcagcc tgatggagcc gaaagaggtt gcggaatccg tgatgttcat gctgacacgc ccgcgcaata tcaccatccg cgatctggtt attcttccgc aaagtctcga tctctaagtg agtaagatag gatccggctg cnnnannncc ccnnnnnnnn nnngnnnaaa annnnnnnnn nnnngnnncc nncnnnnngn cnnnnacann nnncnannan nnnncnggng nncnatnntn nnggnnnnna nnnnnnnnnn nnnnncnntn nnnnnnncna cnnnnatncc acnnccnntn nnncntnnnn nnnnnnnnnn nnnnnngatn nnnnnnnnnn nancaaagcn ttnnnnnnna nnnnnnnnnn tnnnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattg gggcgggcat aaccgtgttt cttgtcgacc gcgacgagaa cgcgcttgcg gaaaagtgcg ttgagcttgg cgacaaggcc aaaccgctgg tcgtcgatct gctcgatccg gcatccgtcg ccaccatgat gccgcagatt cttgaacagg caggccagct cgatattttc cacgccaatg ccggctccta tattggcggg ccgctctggg agggcgatcc ggatgcatgg gatcgcatgt tgaacctcaa tattaatgcg gctttccgta ccgtccatgc cgttctgccg catatgatgg aacgcaagac tggcgacatc atcatgacca gctctgttgc tggcgtcgtg ccggtgatat gggaaccgat ttatacggcg tccaaacatg ccgtgcaggc tttcgtgcac acggttcgcc gtcaggtcgc cccgcatggt attcgcgttg gcggtgttct gccgggcccg gtcgtcactg cgctcatcag cgactggccg caagccaagc tcgatgaggc gctggcggca ggcagcctga tggagccgaa agaggttgcg gaatccgtga tgttcatgct gacacgcccg cgcaatatca ccatccgcga tctggttatt cttccgcaaa gtctcgatct ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat