BrmeB.01123.a

Dihydropyrimidinase (EC 3.5.2.2)

CENTER ID: BrmeB.01123.a
ORGANISM: Brucella melitensis ATCC 23457
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.01123.a.B1.GE36935 Full length( BrmeB.01123.a ) 1 489
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel14466_0328
BV-BRC: fig|546272.3.peg.328
RefSeq: YP_002732059.1
UniProt: C0RGZ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKTGASKVI KGGTVITADR TFRADILIED DKIAAIGDSL EGDEVIDASG CYVMPGGIDP HTHLQMPFMG TYSSDDFDTG TAAALAGGTT MVVDFVLPDS EGNLLDALQE WFQKAGKART DYSFHIAITG WNERTFNEMV EVVKRGINTF KHFMAYKGAL MVNDDEMFAS FQRCAELGAM PLVHAENGDI VAQLQAKLMA EGNDGPEAHA YSRPPEVEGE ATNRAIMIAD QAGVPLYVVH VSCEQSHEAI RRARQKGIRV FGEPLIQHLT LDESEYHNRD WDYAARRVMS PPFRDKLNQD SLWAGLAAGS LQCVATDHCA FTTEQKRYGI GNFTKIPNGT GGLEERMPVL WTRGVRTGRL TPNEFVAVTS TNIAKILNIY PQKGAVVPGA DADLVIWDPE ATKKISAKTQ HSSIDYNVFE GFELKGLPIM TLSRGRIAFD KGQVTAKPGD GRFIEREPNG AVNRALSQWK EIVAPRKVER SAEHMPIGV
NT Sequence
ATGGCCAAGA CCGGGGCAAG CAAAGTCATC AAGGGCGGCA CCGTCATCAC GGCTGACCGC ACGTTCAGGG CAGATATTCT TATCGAAGAC GACAAGATCG CCGCCATTGG CGATAGCCTT GAAGGCGATG AGGTCATCGA CGCATCCGGC TGCTATGTCA TGCCGGGCGG CATCGACCCG CACACCCACC TGCAAATGCC CTTCATGGGC ACATATTCCT CCGACGATTT CGACACGGGC ACGGCGGCAG CACTTGCCGG CGGCACGACG ATGGTGGTCG ATTTCGTCCT GCCGGATTCC GAGGGCAATC TTCTGGATGC GCTTCAGGAA TGGTTTCAGA AGGCGGGCAA GGCGCGCACC GATTATTCGT TCCACATAGC GATCACCGGC TGGAACGAGC GCACTTTCAA CGAAATGGTC GAAGTGGTGA AGCGCGGCAT CAACACGTTC AAGCATTTCA TGGCCTATAA GGGCGCGCTG ATGGTGAATG ACGATGAGAT GTTCGCCTCG TTCCAGCGTT GTGCGGAACT GGGTGCCATG CCGCTCGTCC ATGCGGAAAA CGGCGATATC GTCGCGCAAT TGCAGGCGAA ACTCATGGCC GAAGGCAATG ACGGGCCGGA AGCCCATGCC TATTCCCGCC CGCCGGAAGT GGAAGGAGAA GCCACCAATC GCGCCATCAT GATCGCCGAT CAGGCGGGCG TACCGCTTTA TGTGGTGCAT GTCTCCTGCG AGCAAAGCCA TGAAGCAATC CGCCGCGCGC GCCAGAAGGG TATACGTGTT TTCGGCGAGC CGCTGATCCA GCATCTGACG CTCGATGAAA GCGAATATCA CAATCGCGAC TGGGACTATG CCGCGCGGCG CGTGATGTCG CCGCCGTTCC GCGACAAGCT CAATCAGGAC AGCCTCTGGG CGGGCCTTGC GGCTGGAAGC CTGCAATGCG TGGCGACCGA TCATTGCGCC TTCACCACCG AGCAGAAACG CTATGGCATC GGCAATTTCA CCAAGATTCC GAACGGCACC GGCGGGCTGG AGGAACGTAT GCCGGTTCTG TGGACGCGCG GCGTGCGAAC GGGCCGCCTG ACACCTAATG AGTTCGTGGC CGTCACTTCC ACGAACATCG CGAAAATCCT CAACATCTAT CCGCAGAAAG GTGCCGTGGT GCCAGGCGCG GACGCCGATC TTGTTATATG GGATCCGGAG GCGACCAAGA AGATTTCCGC CAAGACGCAG CATTCCTCCA TCGATTACAA CGTGTTCGAG GGTTTCGAGC TGAAGGGGCT GCCGATCATG ACGCTGTCGC GCGGGCGGAT CGCCTTCGAC AAGGGACAGG TGACAGCAAA ACCGGGCGAC GGGCGCTTCA TCGAGCGTGA GCCGAATGGC GCGGTCAATC GCGCGCTATC GCAATGGAAG GAAATTGTCG CGCCGCGCAA GGTGGAGCGC AGTGCCGAAC ATATGCCGAT AGGAGTC
Details for BrmeB.01123.a.B1.GE36935
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 55kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 68
Validated AA Sequence
MPGGIDPHTH LQMPFMGTYS SDDFDTGTAA ALAGGTTMVV DFVLPDSEGN LLDALQEWFQ KAGKARTDYS FHIAITGWNE RTFNEMVEVV KRGINTFKHF MAYKGALMVN DDEMFASFQR CAELGAMPLV HAENGDIVAQ LQAKLMAEGN DGPEAHAYSR PPEVEGEATN RAIMIADQAG VPLYVVHVSC EQSHEAIRRA RQKGIRVFXE PLIQHLTLDE SEYHNRDWXY AARRVMSPPF RDKLNXDSLW AGLAAGXXQX VATDXXXFXX EQKRYGIGNF TKIPNGXXXL XXXXXXXXTR GVRTXRLTXN EFVAVTSXNI AKILNIYPQK GAXVPGADAD XV
Validated NT Sequence
tataacanga tcggcgtccg cgcctggcac cncggcacct ttctgcggat agatgttgag gattttcgcg atgttcgngg aagtgacggc cacgaactca ttangtgtca ggcggnccgt tcgcacgccg cgngtccnca nanncngnnt anntncntcc agnncnncng ngccgttcgg aatcttggtg aaattgccga tgccatagcg tttctgctcn gnggngaang nncnangatc ggtcgccacn cnttgcangn ntccagccgc aaggcccgcc cagaggctgt ccngattgag cttgtcgcgg aacggcggcg acatcacgcg ccgcgcggca tanncccagt cgcgattgtg atattcgctt tcatcgagcg tcagatgctg gatcagcggc tcgcngaaaa cacgtatacc cttctggcgc gcgcggcgga ttgcttcatg gctttgctcg caggagacat gcaccacata aagcggtacg cccgcctgat cggcgatcat gatggcgcga ttggtggctt ctccttccac ttccggcggg cgggaatagg catgggcttc cggcccgtca ttgccttcgg ccatgagttt cgcctgcaat tgcgcgacga tatcgccgtt ttccgcatgg acgagcggca tggcacccag ttccgcacaa cgctggaacg aggcgaacat ctcatcgtca ttcaccatca gcgcgccctt ataggccatg aaatgcttga acgtgttgat gccgcgcttc accacttcga ccatttcgtt gaaagtgcgc tcgttccagc cggtgatcgc tatgtggaac gaataatcgg tgcgcgcctt gcccgccttc tgaaaccatt cctgaagcgc atccagaaga ttgccctcgg aatccggcag gacgaaatcg accaccatcg tcgtgccgcc ggcaagtgct gccgccgtgc ccgtgtcgaa atcgtcggag gaatatgtgc ccatgaaggg catttgcagg tgggtgtgcg ggtcgatgcc gcccggcatg acatagcagc cggatgcgtc gatgacctca tcgccttcaa ggctatcgcc aatggcggcg atcttgtcgt cttcgataag aatatctgcc ctgaacgtgc ggtcagccgt gatgacggtg ccgcccttga tgactttgct tgccccggtc t
Expected Protein Sequence
MAHHHHHHMA KTGASKVIKG GTVITADRTF RADILIEDDK IAAIGDSLEG DEVIDASGCY VMPGGIDPHT HLQMPFMGTY SSDDFDTGTA AALAGGTTMV VDFVLPDSEG NLLDALQEWF QKAGKARTDY SFHIAITGWN ERTFNEMVEV VKRGINTFKH FMAYKGALMV NDDEMFASFQ RCAELGAMPL VHAENGDIVA QLQAKLMAEG NDGPEAHAYS RPPEVEGEAT NRAIMIADQA GVPLYVVHVS CEQSHEAIRR ARQKGIRVFG EPLIQHLTLD ESEYHNRDWD YAARRVMSPP FRDKLNQDSL WAGLAAGSLQ CVATDHCAFT TEQKRYGIGN FTKIPNGTGG LEERMPVLWT RGVRTGRLTP NEFVAVTSTN IAKILNIYPQ KGAVVPGADA DLVIWDPEAT KKISAKTQHS SIDYNVFEGF ELKGLPIMTL SRGRIAFDKG QVTAKPGDGR FIEREPNGAV NRALSQWKEI VAPRKVERSA EHMPIGV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcca agaccggggc aagcaaagtc atcaagggcg gcaccgtcat cacggctgac cgcacgttca gggcagatat tcttatcgaa gacgacaaga tcgccgccat tggcgatagc cttgaaggcg atgaggtcat cgacgcatcc ggctgctatg tcatgccggg cggcatcgac ccgcacaccc acctgcaaat gcccttcatg ggcacatatt cctccgacga tttcgacacg ggcacggcgg cagcacttgc cggcggcacg acgatggtgg tcgatttcgt cctgccggat tccgagggca atcttctgga tgcgcttcag gaatggtttc agaaggcggg caaggcgcgc accgattatt cgttccacat agcgatcacc ggctggaacg agcgcacttt caacgaaatg gtcgaagtgg tgaagcgcgg catcaacacg ttcaagcatt tcatggccta taagggcgcg ctgatggtga atgacgatga gatgttcgcc tcgttccagc gttgtgcgga actgggtgcc atgccgctcg tccatgcgga aaacggcgat atcgtcgcgc aattgcaggc gaaactcatg gccgaaggca atgacgggcc ggaagcccat gcctattccc gcccgccgga agtggaagga gaagccacca atcgcgccat catgatcgcc gatcaggcgg gcgtaccgct ttatgtggtg catgtctcct gcgagcaaag ccatgaagca atccgccgcg cgcgccagaa gggtatacgt gttttcggcg agccgctgat ccagcatctg acgctcgatg aaagcgaata tcacaatcgc gactgggact atgccgcgcg gcgcgtgatg tcgccgccgt tccgcgacaa gctcaatcag gacagcctct gggcgggcct tgcggctgga agcctgcaat gcgtggcgac cgatcattgc gccttcacca ccgagcagaa acgctatggc atcggcaatt tcaccaagat tccgaacggc accggcgggc tggaggaacg tatgccggtt ctgtggacgc gcggcgtgcg aacgggccgc ctgacaccta atgagttcgt ggccgtcact tccacgaaca tcgcgaaaat cctcaacatc tatccgcaga aaggtgccgt ggtgccaggc gcggacgccg atcttgttat atgggatccg gaggcgacca agaagatttc cgccaagacg cagcattcct ccatcgatta caacgtgttc gagggtttcg agctgaaggg gctgccgatc atgacgctgt cgcgcgggcg gatcgccttc gacaagggac aggtgacagc aaaaccgggc gacgggcgct tcatcgagcg tgagccgaat ggcgcggtca atcgcgcgct atcgcaatgg aaggaaattg tcgcgccgcg caaggtggag cgcagtgccg aacatatgcc gataggagtc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat