BrmeB.01104.b

Cysteine desulfurase (EC 2.8.1.7), SufS subfamily

CENTER ID:	BrmeB.01104.b
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	native diffraction-data
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.01104.b.B1.GE36934	Full length( BrmeB.01104.b )		1	414

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.01104.b.B1.PS01871	Full length( BrmeB.01104.b )		1	414

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|546272.3.peg.989
RefSeq:	YP_002732678.1
UniProt:	C0RIR6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDQKNPLTMA YDVEAIRRDF PILSRQVHGK TLVYLDNGAS AQKPQSVIDA VTHAYANEYA NVHRGLHFLS NAATDAYEKS RETVRRFLNA GSVDEIVFTK NATEAINTVA YGYGMPFIGE GDEILLSIME HHSNIVPWHF IRERQGAKLV FTPVDDNGVF HIEEFEKRLS ERTKLVAITH MSNTLGTVVP IKKIVELAHA RGVPVLVDGS QGAVHLPVDV QDLGCDWYVF TGHKVYGPSG IGVLYGRAQM LEKMRPFQGG GEMIEEVTEE NVTYNHPPHR FEAGTPPIVQ AIGLGAALEY MEKIGRHAIL AHEADLRDYA HERLGRINSL RIFGNAPDKG AIISFALEGI HAHDVSMVID RAGVAVRAGT HCAQPLLKRF GVTSTCRASF ALYNTRAEVD ALAEALEKAR KFFG

NT Sequence

ATGGATCAGA AGAATCCGCT CACCATGGCC TATGATGTCG AGGCCATACG CAGGGATTTC CCGATTTTGT CGCGGCAGGT GCACGGCAAA ACGCTCGTTT ATCTGGATAA CGGCGCTTCC GCACAGAAGC CGCAAAGCGT TATCGACGCG GTAACTCATG CTTACGCGAA TGAATATGCC AATGTTCATC GCGGTTTGCA TTTTCTCTCG AACGCTGCCA CCGACGCTTA TGAGAAGTCG CGTGAAACCG TGCGACGTTT TCTCAATGCC GGTTCTGTGG ACGAGATCGT CTTCACCAAG AATGCGACAG AGGCGATCAA TACCGTCGCT TATGGCTATG GAATGCCGTT CATCGGCGAG GGCGATGAAA TCCTGCTCTC GATCATGGAG CATCATTCCA ACATCGTGCC GTGGCATTTC ATACGCGAAC GCCAGGGCGC GAAACTGGTT TTCACACCAG TCGACGATAA CGGTGTTTTC CACATCGAAG AGTTCGAGAA GCGCCTGAGC GAACGTACCA AACTCGTTGC CATCACGCAT ATGTCGAATA CACTCGGCAC GGTGGTCCCG ATCAAGAAGA TCGTGGAGCT TGCCCATGCA CGCGGCGTTC CGGTTCTGGT CGATGGCAGT CAGGGTGCAG TGCACCTGCC GGTCGATGTG CAGGATCTGG GCTGTGACTG GTATGTCTTT ACCGGCCACA AAGTCTATGG CCCTTCGGGC ATCGGCGTGC TTTATGGACG CGCGCAAATG CTGGAAAAGA TGCGCCCGTT CCAGGGCGGG GGCGAAATGA TCGAGGAAGT GACGGAGGAA AACGTCACCT ACAATCATCC GCCACACCGC TTTGAAGCAG GCACGCCGCC TATCGTTCAG GCGATTGGTC TGGGTGCGGC GCTTGAATAT ATGGAAAAGA TCGGGCGTCA TGCGATCCTG GCTCACGAGG CAGACTTGCG TGATTATGCG CATGAACGGC TCGGCCGGAT CAACAGCTTG CGCATTTTCG GCAATGCGCC GGACAAGGGC GCAATCATCT CCTTCGCGCT TGAAGGCATT CATGCCCATG ACGTGTCGAT GGTGATCGAT CGGGCGGGGG TCGCGGTGCG CGCGGGAACG CATTGTGCGC AACCGCTCTT GAAACGCTTT GGCGTCACCT CTACATGCCG TGCATCCTTT GCCCTTTATA ATACCCGCGC CGAAGTAGAC GCATTGGCTG AAGCCTTGGA AAAGGCAAGG AAGTTTTTCG GA

Details for BrmeB.01104.b.B1.GE36934

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	47kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	92
PERCENT COVERAGE:	93

Validated AA Sequence

MAYDVEAIRR DFPILSRQVH GKTLVYLDNG ASAQKPQSVI DAVTHAYANE YANVHRGLHF LSNAATDAYE KSRETVRRFL NAGSVDEIVF TKNATEAINT VAYGYGMPFI GEGDEILLSI MEHHSNIVPW HFIRERQGAK LVFTPVDDNG VFHIEEFEKR LSERTKLVAI THMSNTLGTV VPIKKIVELA HARGVPVLVD GSQGAVHLPV DVQDLGCDWY VFTGHKVYGP SGIGVLYGRA QMLEKMRPFQ GGGEMIEEVT EENVTYNHPP HRFEAXTPPI VQXXXXGAAL EYMEXXGXXA ILAHEADXXD XXHERLGRIX SLRXXXNAPD KGAIXSXALE GIHXHXXXMV IDRAGVAXRA GTHXXQPLXK RXGVTSTXRA SFALYNTRAE VDAX

Validated NT Sequence

ccnatgcgtc tacttcggcg cgggtattat aaagggcaaa ggatgcacgg natgtagagg tgacnccnaa gcgtttnnng agcggttgcn cncaatgcgt tcccgcgcgc nccgcgaccc ccgcccgatc gatcaccatc nnnncnncat gggnatgaat gccttcnagc gcnnangana tgattgcgcc cttgtccggc gcattgncna nnatgcgcaa gctgntgatc cggccgagcc gttcatgcnn nnaatcncnc nngtctgcct cgtgagccag gatcgcnngn nncccgannn nttccatata ttcaagcgcc gcacccnnnn nnntcncctg aacgataggc ggcgtgnctg cttcaaagcg gtgtggcgga tgattgtagg tgacgttttc ctccgtcact tcctcgatca tttcgccncc gccctggaac gggcgcatct tttccagcat ttgcgcgcgt ccataaagca cgccgatgcc cgaagggcca tagactttgt ggccggtaaa gacataccag tcacagccca gatcctgcac atcgaccggc aggtgcactg caccctgact gccatcgacc agaaccggaa cgccgcgtgc atgggcaagc tccacgatct tcttgatcgg gaccaccgtg ccgagtgtat tcgacatatg cgtgatggca acgagtttgg tacgttcgct caggcgcttc tcgaactctt cgatgtggaa aacaccgtta tcgtcgactg gtgtgaaaac cagtttcgcg ccctggcgtt cgcgtatgaa atgccacggc acgatgttgg aatgatgctc catgatcgag agcaggattt catcgccctc gccgatgaac ggcattccat agccataagc gacggtattg atcgcctctg tcgcattctt ggtgaagacg atctcgtcca cagaaccggc attgagaaaa cgtcgcacgg tttcacgcga cttctcataa gcgtcggtgg cagcgttcga gagaaaatgc aaaccgcgat gaacattggc atattcattc gcgtaagcat gagttaccgc gtcgataacg ctttgcggct tctgtgcgga agcgccgtta tccagataaa cgagcgtttt gccgtgcacc tgccgcgaca aaatcgggaa atccctgcgt atggcctcga catcataggc catggtgagc ggattcttct g

Expected Protein Sequence

MAHHHHHHMD QKNPLTMAYD VEAIRRDFPI LSRQVHGKTL VYLDNGASAQ KPQSVIDAVT HAYANEYANV HRGLHFLSNA ATDAYEKSRE TVRRFLNAGS VDEIVFTKNA TEAINTVAYG YGMPFIGEGD EILLSIMEHH SNIVPWHFIR ERQGAKLVFT PVDDNGVFHI EEFEKRLSER TKLVAITHMS NTLGTVVPIK KIVELAHARG VPVLVDGSQG AVHLPVDVQD LGCDWYVFTG HKVYGPSGIG VLYGRAQMLE KMRPFQGGGE MIEEVTEENV TYNHPPHRFE AGTPPIVQAI GLGAALEYME KIGRHAILAH EADLRDYAHE RLGRINSLRI FGNAPDKGAI ISFALEGIHA HDVSMVIDRA GVAVRAGTHC AQPLLKRFGV TSTCRASFAL YNTRAEVDAL AEALEKARKF FG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc agaagaatcc gctcaccatg gcctatgatg tcgaggccat acgcagggat ttcccgattt tgtcgcggca ggtgcacggc aaaacgctcg tttatctgga taacggcgct tccgcacaga agccgcaaag cgttatcgac gcggtaactc atgcttacgc gaatgaatat gccaatgttc atcgcggttt gcattttctc tcgaacgctg ccaccgacgc ttatgagaag tcgcgtgaaa ccgtgcgacg ttttctcaat gccggttctg tggacgagat cgtcttcacc aagaatgcga cagaggcgat caataccgtc gcttatggct atggaatgcc gttcatcggc gagggcgatg aaatcctgct ctcgatcatg gagcatcatt ccaacatcgt gccgtggcat ttcatacgcg aacgccaggg cgcgaaactg gttttcacac cagtcgacga taacggtgtt ttccacatcg aagagttcga gaagcgcctg agcgaacgta ccaaactcgt tgccatcacg catatgtcga atacactcgg cacggtggtc ccgatcaaga agatcgtgga gcttgcccat gcacgcggcg ttccggttct ggtcgatggc agtcagggtg cagtgcacct gccggtcgat gtgcaggatc tgggctgtga ctggtatgtc tttaccggcc acaaagtcta tggcccttcg ggcatcggcg tgctttatgg acgcgcgcaa atgctggaaa agatgcgccc gttccagggc gggggcgaaa tgatcgagga agtgacggag gaaaacgtca cctacaatca tccgccacac cgctttgaag caggcacgcc gcctatcgtt caggcgattg gtctgggtgc ggcgcttgaa tatatggaaa agatcgggcg tcatgcgatc ctggctcacg aggcagactt gcgtgattat gcgcatgaac ggctcggccg gatcaacagc ttgcgcattt tcggcaatgc gccggacaag ggcgcaatca tctccttcgc gcttgaaggc attcatgccc atgacgtgtc gatggtgatc gatcgggcgg gggtcgcggt gcgcgcggga acgcattgtg cgcaaccgct cttgaaacgc tttggcgtca cctctacatg ccgtgcatcc tttgcccttt ataatacccg cgccgaagta gacgcattgg ctgaagcctt ggaaaaggca aggaagtttt tcggatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for BrmeB.01104.b.B1.PS01871

PURIFICATION DATe:	7/31/2013
CONCENTRATION:	24mg/ml
OBSERVED MW:	45kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	92
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGATC AGAAGAATCC GCTCACCATG GCCTATGATG TCGAGGCCAT ACGCAGGGAT TTCCCGATTT TGTCGCGGCA GGTGCACGGC AAAACGCTCG TTTATCTGGA TAACGGCGCT TCCGCACAGA AGCCGCAAAG CGTTATCGAC GCGGTAACTC ATGCTTACGC GAATGAATAT GCCAATGTTC ATCGCGGTTT GCATTTTCTC TCGAACGCTG CCACCGACGC TTATGAGAAG TCGCGTGAAA CCGTGCGACG TTTTCTCAAT GCCGGTTCTG TGGACGAGAT CGTCTTCACC AAGAATGCGA CAGAGGCGAT CAATACCGTC GCTTATGGCT ATGGAATGCC GTTCATCGGC GAGGGCGATG AAATCCTGCT CTCGATCATG GAGCATCATT CCAACATCGT GCCGTGGCAT TTCATACGCG AACGCCAGGG CGCGAAACTG GTTTTCACAC CAGTCGACGA TAACGGTGTT TTCCACATCG AAGAGTTCGA GAAGCGCCTG AGCGAACGTA CCAAACTCGT TGCCATCACG CATATGTCGA ATACACTCGG CACGGTGGTC CCGATCAAGA AGATCGTGGA GCTTGCCCAT GCACGCGGCG TTCCGGTTCT GGTCGATGGC AGTCAGGGTG CAGTGCACCT GCCGGTCGAT GTGCAGGATC TGGGCTGTGA CTGGTATGTC TTTACCGGCC ACAAAGTCTA TGGCCCTTCG GGCATCGGCG TGCTTTATGG ACGCGCGCAA ATGCTGGAAA AGATGCGCCC GTTCCAGGGC GGGGGCGAAA TGATCGAGGA AGTGACGGAG GAAAACGTCA CCTACAATCA TCCGCCACAC CGCTTTGAAG CAGGCACGCC GCCTATCGTT CAGGCGATTG GTCTGGGTGC GGCGCTTGAA TATATGGAAA AGATCGGGCG TCATGCGATC CTGGCTCACG AGGCAGACTT GCGTGATTAT GCGCATGAAC GGCTCGGCCG GATCAACAGC TTGCGCATTT TCGGCAATGC GCCGGACAAG GGCGCAATCA TCTCCTTCGC GCTTGAAGGC ATTCATGCCC ATGACGTGTC GATGGTGATC GATCGGGCGG GGGTCGCGGT GCGCGCGGGA ACGCATTGTG CGCAACCGCT CTTGAAACGC TTTGGCGTCA CCTCTACATG CCGTGCATCC TTTGCCCTTT ATAATACCCG CGCCGAAGTA GACGCATTGG CTGAAGCCTT GGAAAAGGCA AGGAAGTTTT TCGGATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT