BrmeB.01104.a

Cysteine desulfurase (EC 2.8.1.7)

CENTER ID: BrmeB.01104.a
ORGANISM: Brucella melitensis ATCC 23457
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.01104.a.B1.GE36933 Full length( BrmeB.01104.a ) 1 430
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel14466_0985
BV-BRC: fig|546272.3.peg.985
RefSeq: YP_002732674.1
UniProt: C0RIR2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTETGFSFSS GFIGRSWLKI QENCDFRYLE GVVKEIGKVV GSGKRLYLDY NASAPLLDEA RDAIIAALGI TGNPSSVHRE GRAARALVES ARRSVATLVN ARPEHVFFTS GATEAASTLL TPNYMMGRSS VRLSHLYVSA TEHPCMIAGG QFSPDDITVL PVDANGILRL DILRGLLAAH DRSKGLPLVA VQAANNETGV IQPVAEIAAI VKAAGGIYIV DAVQAAGRIK LDITDNCGDY LIISSHKIGG PKGVGAVIAI SDLMMPRALV RGGGQEKGHR AGTEALPLIA GFGAAADVAK ARIDGEGWSS LMRDRLERGL AEIAPAAIVH GSAVPRLPNT TFFSLQGQKA ETVQIAFDLA GIALSAGSAC SSGKVGPSHV LAAMGQEDGP GAIRVSLATD AGEETVDRFL EALRKIVERH QRTKHELGAV
NT Sequence
ATGACTGAAA CAGGTTTCAG TTTCTCAAGC GGCTTCATAG GCCGTTCTTG GTTGAAAATT CAAGAAAATT GCGACTTCAG ATATCTCGAA GGCGTTGTTA AGGAGATAGG TAAGGTGGTC GGGAGCGGCA AGAGGCTCTA TCTGGATTAT AATGCCAGTG CGCCTTTGCT TGATGAAGCG CGCGATGCGA TCATCGCGGC ATTGGGCATT ACGGGCAATC CATCCTCCGT TCACCGCGAG GGGCGGGCTG CCCGTGCGCT GGTCGAATCC GCCCGCCGCA GTGTTGCGAC CCTCGTTAAT GCCAGGCCGG AGCATGTCTT TTTTACGTCC GGTGCGACAG AGGCGGCCTC GACGCTGCTG ACCCCCAATT ACATGATGGG GCGTTCGTCG GTGCGTCTCT CACACCTTTA TGTCAGCGCC ACCGAACACC CATGCATGAT CGCCGGCGGA CAGTTTTCGC CCGATGACAT CACCGTTCTT CCGGTTGATG CCAATGGCAT CCTGCGCCTG GACATTTTGC GCGGCCTGCT TGCCGCCCAT GACAGGAGCA AGGGTCTTCC GCTGGTTGCA GTACAGGCAG CCAATAATGA AACGGGCGTG ATCCAGCCGG TGGCGGAGAT CGCAGCCATC GTAAAGGCTG CGGGCGGCAT TTACATCGTC GATGCCGTGC AGGCTGCAGG GAGAATTAAA TTAGATATTA CAGATAATTG CGGTGATTAT CTCATAATTT CCTCACACAA GATCGGTGGC CCGAAGGGCG TCGGCGCGGT CATTGCGATC TCCGACCTCA TGATGCCGCG GGCGCTGGTG CGCGGCGGCG GGCAGGAAAA AGGCCACCGG GCGGGAACAG AGGCGCTGCC CCTGATTGCC GGTTTTGGTG CGGCTGCCGA TGTGGCAAAA GCGCGAATCG ACGGCGAAGG CTGGTCATCC CTCATGCGGG ATCGTCTGGA GCGTGGCCTT GCCGAAATTG CGCCAGCGGC GATCGTTCAT GGCAGTGCTG TGCCGCGCTT GCCGAATACG ACTTTCTTTT CCCTGCAAGG CCAGAAGGCT GAAACGGTGC AGATCGCTTT TGATCTTGCA GGTATTGCGC TTTCGGCCGG GTCGGCCTGT TCTTCGGGCA AGGTGGGGCC GAGCCATGTT CTTGCTGCCA TGGGGCAGGA AGACGGGCCG GGAGCCATCC GTGTTTCGCT TGCAACGGAT GCGGGCGAGG AAACAGTGGA TCGATTTCTT GAAGCCTTGC GCAAAATCGT CGAGCGTCAC CAGCGCACGA AGCACGAATT GGGCGCGGTT
Details for BrmeB.01104.a.B1.GE36933
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 46kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 41
Validated AA Sequence
MMGRSSVRLS HLYVSATEHP CMIAGGQFSP DDITVLPVDA NGILRLDILR GLLAAHDRSK GLPLVAVQAA NNETGVIQPV AEIAAIVKAA GGIYIVDAVQ AAGRIKLDIT DNCGDYLIIS SHKIGGPXGV XAVIAISDLM MPRALVRGGG QEKGHRAGTE XLPLXAGFGA AADVAKARID G
Validated NT Sequence
tcgccgtcga ttcgcgcttt tgccacatcg gcagccgcac caaaaccggc antcaggggc agcncctctg ttcccgcccg gtggcctttt tcctgcccgc cgccgcgcac cagcgcccgc ggcatcatga ggtcggagat cgcaatgacc gcgcngacgc cnttcgggcc accgatcttg tgtgaggaaa ttatgagata atcaccgcaa ttatctgtaa tatctaattt aattctccct gcagcctgca cggcatcgac gatgtaaatg ccgcccgcag cctttacgat ggctgcgatc tccgccaccg gctggatcac gcccgtttca ttattggctg cctgtactgc aaccagcgga agacccttgc tcctgtcatg ggcggcaagc aggccgcgca aaatgtccag gcgcaggatg ccattggcat caaccggaag aacggtgatg tcatcgggcg aaaactgtcc gccggcgatc atgcatgggt gttcggtggc gctgacataa aggtgtgaga gacgcaccga cgaacgcccc atcatgtaat tgggggtcag cagcgtcgag gccgcctctg tcgcaccgga cgtaaaaaag acatgctccg gcctggcatt aacgagggtc gcaacactgc ggcgggcgga ttcgaccagc gcacgggcag cccgcccctc gcggtgaacg gaggatggat tgcccgtaat gcccaatgcc gcgatgatcg catcgcgcgc ttcatcaagc aaaggcgcac tggcattata atccagatag agcctcttgc cgctcccgac caccttacct atctccttaa caacgccttc gagatatctg aagtcgcaat tttcttgaat tttcaaccaa gaacggccta tgaagccgct tgagaaactg aaacctgtt
Expected Protein Sequence
MAHHHHHHMT ETGFSFSSGF IGRSWLKIQE NCDFRYLEGV VKEIGKVVGS GKRLYLDYNA SAPLLDEARD AIIAALGITG NPSSVHREGR AARALVESAR RSVATLVNAR PEHVFFTSGA TEAASTLLTP NYMMGRSSVR LSHLYVSATE HPCMIAGGQF SPDDITVLPV DANGILRLDI LRGLLAAHDR SKGLPLVAVQ AANNETGVIQ PVAEIAAIVK AAGGIYIVDA VQAAGRIKLD ITDNCGDYLI ISSHKIGGPK GVGAVIAISD LMMPRALVRG GGQEKGHRAG TEALPLIAGF GAAADVAKAR IDGEGWSSLM RDRLERGLAE IAPAAIVHGS AVPRLPNTTF FSLQGQKAET VQIAFDLAGI ALSAGSACSS GKVGPSHVLA AMGQEDGPGA IRVSLATDAG EETVDRFLEA LRKIVERHQR TKHELGAV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg aaacaggttt cagtttctca agcggcttca taggccgttc ttggttgaaa attcaagaaa attgcgactt cagatatctc gaaggcgttg ttaaggagat aggtaaggtg gtcgggagcg gcaagaggct ctatctggat tataatgcca gtgcgccttt gcttgatgaa gcgcgcgatg cgatcatcgc ggcattgggc attacgggca atccatcctc cgttcaccgc gaggggcggg ctgcccgtgc gctggtcgaa tccgcccgcc gcagtgttgc gaccctcgtt aatgccaggc cggagcatgt cttttttacg tccggtgcga cagaggcggc ctcgacgctg ctgaccccca attacatgat ggggcgttcg tcggtgcgtc tctcacacct ttatgtcagc gccaccgaac acccatgcat gatcgccggc ggacagtttt cgcccgatga catcaccgtt cttccggttg atgccaatgg catcctgcgc ctggacattt tgcgcggcct gcttgccgcc catgacagga gcaagggtct tccgctggtt gcagtacagg cagccaataa tgaaacgggc gtgatccagc cggtggcgga gatcgcagcc atcgtaaagg ctgcgggcgg catttacatc gtcgatgccg tgcaggctgc agggagaatt aaattagata ttacagataa ttgcggtgat tatctcataa tttcctcaca caagatcggt ggcccgaagg gcgtcggcgc ggtcattgcg atctccgacc tcatgatgcc gcgggcgctg gtgcgcggcg gcgggcagga aaaaggccac cgggcgggaa cagaggcgct gcccctgatt gccggttttg gtgcggctgc cgatgtggca aaagcgcgaa tcgacggcga aggctggtca tccctcatgc gggatcgtct ggagcgtggc cttgccgaaa ttgcgccagc ggcgatcgtt catggcagtg ctgtgccgcg cttgccgaat acgactttct tttccctgca aggccagaag gctgaaacgg tgcagatcgc ttttgatctt gcaggtattg cgctttcggc cgggtcggcc tgttcttcgg gcaaggtggg gccgagccat gttcttgctg ccatggggca ggaagacggg ccgggagcca tccgtgtttc gcttgcaacg gatgcgggcg aggaaacagt ggatcgattt cttgaagcct tgcgcaaaat cgtcgagcgt caccagcgca cgaagcacga attgggcgcg gtttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat