SSGCID: Target Page

BrmeB.00808.a

L-asparaginase (EC 3.5.1.1)

CENTER ID:	BrmeB.00808.a
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.00808.a.B1.GE36932	Full length( BrmeB.00808.a )		1	290

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_2033
BV-BRC:	fig\|546272.3.peg.2033
RefSeq:	YP_002733631.1
UniProt:	C0RFK5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPTLTADALI AAVPQIRNYA QIKAQSLFQL PSPSLKFRHG LEVIGWAENE VKSGADGIVL TQGTDTLEEM AFLLDLLWEH PQPLVLTGAM RSPRAPGADG PANLVAAVLT VASRLSRERG VLVAMNDTIH AARWITKSHA LSVQTFVSSD SGPLGYVYEE TPVYINTLSR LPKIDRTQFN ADVKIGFYES LLDGDAQMLR SMFESGRYDG IVVAGFGAGH VSGDEADIIE RYASRIPVVV ASRTYGGRTA TKTYGFHGSE IDLQTKGAML AGWLSPPQGA VIAVGTTGSG

NT Sequence

GTGCCGACCC TGACCGCGGA TGCACTCATT GCCGCTGTGC CGCAGATTAG AAATTATGCG CAGATCAAGG CCCAATCTCT TTTTCAGCTT CCCAGCCCCT CGCTAAAATT TCGGCACGGC CTTGAAGTGA TAGGCTGGGC TGAAAACGAG GTGAAATCGG GAGCGGACGG CATAGTGCTG ACGCAGGGAA CCGATACGCT TGAGGAAATG GCATTCCTTC TGGATTTATT GTGGGAACAT CCGCAGCCCC TCGTACTGAC CGGCGCCATG CGCTCGCCGC GCGCGCCGGG TGCTGACGGT CCGGCCAATC TGGTGGCGGC CGTATTGACA GTCGCGAGCA GGCTTAGCCG GGAACGCGGT GTCCTTGTTG CAATGAACGA TACAATCCAC GCGGCACGTT GGATAACAAA ATCTCATGCG CTCAGTGTCC AGACATTCGT ATCGTCGGAC AGCGGGCCGT TGGGATATGT GTATGAGGAG ACGCCGGTTT ACATCAACAC CCTTTCGCGG TTGCCAAAAA TTGACCGTAC GCAGTTCAAC GCCGATGTGA AGATCGGCTT TTACGAATCG CTGCTGGATG GTGACGCGCA GATGCTGCGA AGCATGTTTG AGAGCGGCAG ATATGATGGA ATCGTCGTCG CCGGTTTTGG AGCAGGACAT GTGTCGGGCG ACGAAGCCGA CATTATCGAG CGCTACGCGT CCCGCATTCC GGTCGTCGTA GCCAGCCGGA CCTATGGCGG AAGGACCGCC ACGAAGACTT ATGGGTTCCA TGGCTCCGAG ATTGATCTTC AGACGAAAGG CGCTATGCTC GCAGGCTGGC TATCCCCCCC GCAAGGCGCG GTTATTGCTG TGGGCACTAC TGGCAGCGGG

Details for BrmeB.00808.a.B1.GE36932

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV PTLTADALIA AVPQIRNYAQ IKAQSLFQLP SPSLKFRHGL EVIGWAENEV KSGADGIVLT QGTDTLEEMA FLLDLLWEHP QPLVLTGAMR SPRAPGADGP ANLVAAVLTV ASRLSRERGV LVAMNDTIHA ARWITKSHAL SVQTFVSSDS GPLGYVYEET PVYINTLSRL PKIDRTQFNA DVKIGFYESL LDGDAQMLRS MFESGRYDGI VVAGFGAGHV SGDEADIIER YASRIPVVVA SRTYGGRTAT KTYGFHGSEI DLQTKGAMLA XWLSPPQGAV IAVGTTGSG

Validated NT Sequence

tttgtnnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatggtgcc gaccctgacc gcggatgcac tcattgccgc tgtgccgcag attagaaatt atgcgcagat caaggcccaa tctctttttc agcttcccag cccctcgcta aaatttcggc acggccttga agtgataggc tgggctgaaa acgaggtgaa atcgggagcg gacggcatag tgctgacgca gggaaccgat acgcttgagg aaatggcatt ccttctggat ttattgtggg aacatccgca gcccctcgta ctgaccggcg ccatgcgctc gccgcgcgcg ccgggtgctg acggtccggc caatctggtg gcggccgtat tgacagtcgc gagcaggctt agccgggaac gcggtgtcct tgttgcaatg aacgatacaa tccacgcggc acgttggata acaaaatctc atgcgctcag tgtccagaca ttcgtatcgt cggacagcgg gccgttggga tatgtgtatg aggagacgcc ggtttacatc aacacccttt cgcggttgcc aaaaattgac cgtacgcagt tcaacgccga tgtgaagatc ggcttttacg aatcgctgct ggatggtgac gcgcagatgc tgcgaagcat gtttgagagc ggcagatatg atggaatcgt cgtcgccggt tttggagcag gacatgtgtc gggcgacgaa gccgacatta tcgagcgcta cgcgtcccgc attccggtcg tcgtagccag ccggacctat ggcggaagga ccgccacgaa gacttatggg ttccatggct ccgagattga tcttcagacg aaaggcgcta tgctcgcang ctggctatcc cccccgcaag gcgcggttat tgctgtgggc actactggca gcgggtaagt gagtaanata ggancnnnnn nnnnnnnccn nnnnnnnnnn nnnnnnnnan nnnannnnnn tnnnnnnnnn nnncnnnc

Expected Protein Sequence

MAHHHHHHMP TLTADALIAA VPQIRNYAQI KAQSLFQLPS PSLKFRHGLE VIGWAENEVK SGADGIVLTQ GTDTLEEMAF LLDLLWEHPQ PLVLTGAMRS PRAPGADGPA NLVAAVLTVA SRLSRERGVL VAMNDTIHAA RWITKSHALS VQTFVSSDSG PLGYVYEETP VYINTLSRLP KIDRTQFNAD VKIGFYESLL DGDAQMLRSM FESGRYDGIV VAGFGAGHVS GDEADIIERY ASRIPVVVAS RTYGGRTATK TYGFHGSEID LQTKGAMLAG WLSPPQGAVI AVGTTGSG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgccga ccctgaccgc ggatgcactc attgccgctg tgccgcagat tagaaattat gcgcagatca aggcccaatc tctttttcag cttcccagcc cctcgctaaa atttcggcac ggccttgaag tgataggctg ggctgaaaac gaggtgaaat cgggagcgga cggcatagtg ctgacgcagg gaaccgatac gcttgaggaa atggcattcc ttctggattt attgtgggaa catccgcagc ccctcgtact gaccggcgcc atgcgctcgc cgcgcgcgcc gggtgctgac ggtccggcca atctggtggc ggccgtattg acagtcgcga gcaggcttag ccgggaacgc ggtgtccttg ttgcaatgaa cgatacaatc cacgcggcac gttggataac aaaatctcat gcgctcagtg tccagacatt cgtatcgtcg gacagcgggc cgttgggata tgtgtatgag gagacgccgg tttacatcaa caccctttcg cggttgccaa aaattgaccg tacgcagttc aacgccgatg tgaagatcgg cttttacgaa tcgctgctgg atggtgacgc gcagatgctg cgaagcatgt ttgagagcgg cagatatgat ggaatcgtcg tcgccggttt tggagcagga catgtgtcgg gcgacgaagc cgacattatc gagcgctacg cgtcccgcat tccggtcgtc gtagccagcc ggacctatgg cggaaggacc gccacgaaga cttatgggtt ccatggctcc gagattgatc ttcagacgaa aggcgctatg ctcgcaggct ggctatcccc cccgcaaggc gcggttattg ctgtgggcac tactggcagc gggtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat