BrmeB.00674.b

Aspartate aminotransferase (EC 2.6.1.1)

CENTER ID: BrmeB.00674.b
ORGANISM: Brucella melitensis ATCC 23457
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.00674.b.B1.GE36931 Full length( BrmeB.00674.b ) 1 406
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel14466_1341
BV-BRC: fig|546272.3.peg.1341
RefSeq: YP_002733002.1
UniProt: C0RJP0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEEFHKIRR LPHYVFEQVN RLKASARAAG ADIIDLGMGN PDLPTPQNIV DKLCEAVQDP RAHRYSASKG IPGLRRAQAQ YYARRFGVKL NPDTQVVATL GSKEGFANMA QAITAPGDVV LCPDPTYPIH SFGFIMSGGV IRSVQAKPDD NFIPTLERGV KHSIPKPIAL ILNFPSNPTA YVATLDFYKD VVAFARKHDI VILSDLAYSE IYFDGNPPPS VLEVPGAMDV TVEFTSMSKT FSMPGWRMGF AVGNERLIAA LTRVKSYLDY GAFTPIQVAA TAALNGDGSD IAYVRNVYKQ RRDVLVESFG RAGWDVPPPA ATMFAWVPIP ERFRSLGSLE FSKLLVEQAD VAVAPGVGFG EHGDDYVRIA LVENEHRIRQ AARNIKRFFS AKDENLQNVI PLEGHR
NT Sequence
ATGTCGGAAG AATTCCATAA AATCCGTCGT CTGCCACATT ATGTCTTTGA ACAGGTCAAC CGTCTGAAGG CATCGGCGCG AGCGGCGGGT GCCGACATTA TCGATCTGGG CATGGGTAAT CCCGATCTGC CGACCCCGCA AAATATCGTG GACAAGCTTT GCGAGGCTGT GCAGGACCCG CGCGCCCATC GCTATTCCGC CTCCAAGGGC ATTCCGGGCC TGCGCCGTGC TCAGGCACAA TATTATGCAC GCCGTTTTGG TGTGAAGCTT AACCCGGACA CGCAGGTTGT CGCCACGCTT GGCTCCAAGG AAGGCTTCGC CAATATGGCG CAGGCCATCA CCGCGCCGGG CGATGTGGTT CTGTGCCCGG ACCCGACCTA TCCGATCCAC TCCTTTGGCT TCATCATGTC GGGCGGTGTC ATTCGCTCGG TGCAGGCCAA GCCGGACGAT AATTTCATCC CGACGCTTGA GCGCGGCGTG AAACACTCCA TTCCGAAGCC GATTGCGCTG ATCCTCAATT TTCCGTCCAA CCCGACGGCT TATGTGGCGA CGCTTGATTT CTACAAGGAC GTGGTGGCTT TTGCGCGCAA GCACGACATC GTCATCCTTT CGGACCTGGC CTATTCGGAA ATCTATTTCG ACGGCAACCC GCCGCCATCC GTTCTGGAAG TGCCGGGCGC AATGGATGTG ACGGTGGAGT TCACCTCCAT GTCGAAGACT TTCTCCATGC CCGGCTGGCG CATGGGCTTC GCGGTCGGCA ATGAGCGTTT GATCGCCGCG CTGACGCGGG TGAAATCCTA TCTCGATTAT GGCGCCTTTA CGCCGATCCA GGTGGCGGCG ACGGCAGCAC TCAATGGTGA CGGCTCCGAC ATTGCCTATG TGCGCAATGT CTATAAGCAG CGCCGTGACG TGCTGGTTGA AAGCTTCGGC CGCGCTGGCT GGGATGTTCC GCCACCGGCT GCCACCATGT TTGCCTGGGT TCCGATCCCG GAACGGTTCC GCTCGCTGGG TTCGCTGGAA TTCTCCAAAC TTCTGGTGGA GCAGGCCGAT GTGGCTGTTG CGCCGGGCGT CGGTTTTGGT GAACATGGCG ACGATTATGT GCGCATCGCG CTGGTGGAAA ACGAGCATCG CATTCGTCAG GCCGCGCGCA ATATCAAGCG GTTCTTTTCT GCCAAGGATG AGAACCTGCA AAATGTGATT CCGCTTGAAG GTCACCGC
Details for BrmeB.00674.b.B1.GE36931
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMS EEFHKIRRLP HYVFEQVNRL KASARAAGAD IIDLGMGNPD LPTPQNIVDK LCEAVQDPRA HRYSASKGIP GLRRAQAQYY ARRFGVKLNP DTQVVATLGS KEGFANMAQA ITAPGDVVLC PDPTYPIHSF GFIMSGGVIR SVQAKPDDNF IPTLERGVKH SIPKPIALIL NFPSNPTAYV ATLDFYKDVV AFARKHDIVI LSDLAYSEIY FDGNPPPSVL EVPGAMDVTV EFTSMSKTFS MPGWRMGFAV GNERLIAALT RVKSYLDYGA FTPIQVAATA ALXGDGSDIA YVRNXYXQRR DVLVESFXRA GWXVPPPAAX XXAWXXSXNG XLAGXAXXXN FXXXXXXXXX XXXXXXAXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
ttgttnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatgtcggaa gaattccata aaatccgtcg tctgccacat tatgtctttg aacaggtcaa ccgtctgaag gcatcggcgc gagcggcggg tgccgacatt atcgatctgg gcatgggtaa tcccgatctg ccgaccccgc aaaatatcgt ggacaagctt tgcgaggctg tgcaggaccc gcgcgcccat cgctattccg cctccaaggg cattccgggc ctgcgccgtg ctcaggcaca atattatgca cgccgttttg gtgtgaagct taacccggac acgcaggttg tcgccacgct tggctccaag gaaggcttcg ccaatatggc gcaggccatc accgcgccgg gcgatgtggt tctgtgcccg gacccgacct atccgatcca ctcctttggc ttcatcatgt cgggcggtgt cattcgctcg gtgcaggcca agccggacga taatttcatc ccgacgcttg agcgcggcgt gaaacactcc attccgaagc cgattgcgct gatcctcaat tttccgtcca acccgacggc ttatgtggcg acgcttgatt tctacaagga cgtggtggct tttgcgcgca agcacgacat cgtcatcctt tcggacctgg cctattcgga aatctatttc gacggcaacc cgccgccatc cgttctggaa gtgccgggcg caatggatgt gacggtggag ttcacctcca tgtcgaagac tttctccatg cccggctggc gcatgggctt cgcggtcggc aatgagcgtt tgatcgccgc gctgacgcgg gtgaaatcct atctcgatta tggcgccttt acgccgatcc aggtggcggc gacggcagca ctcnatggtg acggctccga cattgcctat gtgcgcaatg nctataanca gcgccgtgac gtgctggttg aaagcttcgn ccgcgctggc tgggnngttc cgccaccggc tgccancntg nttgcctggg nncnatccnn gaacggtnnn ctcgctgggn tcgctgnnnn nncnaacttc ngngnncngn cgannnngnt ntncnnnncn nngnttngnn annnggcnna tnannngnnn nnnnnnntnn nnnaannnnn ncnnnnnnnn annnnnnnnn nnnnnnnnnn nnnnnnnnna antnnnnnnn ngnnnnnnnn nnnnnnnnna
Expected Protein Sequence
MAHHHHHHMS EEFHKIRRLP HYVFEQVNRL KASARAAGAD IIDLGMGNPD LPTPQNIVDK LCEAVQDPRA HRYSASKGIP GLRRAQAQYY ARRFGVKLNP DTQVVATLGS KEGFANMAQA ITAPGDVVLC PDPTYPIHSF GFIMSGGVIR SVQAKPDDNF IPTLERGVKH SIPKPIALIL NFPSNPTAYV ATLDFYKDVV AFARKHDIVI LSDLAYSEIY FDGNPPPSVL EVPGAMDVTV EFTSMSKTFS MPGWRMGFAV GNERLIAALT RVKSYLDYGA FTPIQVAATA ALNGDGSDIA YVRNVYKQRR DVLVESFGRA GWDVPPPAAT MFAWVPIPER FRSLGSLEFS KLLVEQADVA VAPGVGFGEH GDDYVRIALV ENEHRIRQAA RNIKRFFSAK DENLQNVIPL EGHR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgg aagaattcca taaaatccgt cgtctgccac attatgtctt tgaacaggtc aaccgtctga aggcatcggc gcgagcggcg ggtgccgaca ttatcgatct gggcatgggt aatcccgatc tgccgacccc gcaaaatatc gtggacaagc tttgcgaggc tgtgcaggac ccgcgcgccc atcgctattc cgcctccaag ggcattccgg gcctgcgccg tgctcaggca caatattatg cacgccgttt tggtgtgaag cttaacccgg acacgcaggt tgtcgccacg cttggctcca aggaaggctt cgccaatatg gcgcaggcca tcaccgcgcc gggcgatgtg gttctgtgcc cggacccgac ctatccgatc cactcctttg gcttcatcat gtcgggcggt gtcattcgct cggtgcaggc caagccggac gataatttca tcccgacgct tgagcgcggc gtgaaacact ccattccgaa gccgattgcg ctgatcctca attttccgtc caacccgacg gcttatgtgg cgacgcttga tttctacaag gacgtggtgg cttttgcgcg caagcacgac atcgtcatcc tttcggacct ggcctattcg gaaatctatt tcgacggcaa cccgccgcca tccgttctgg aagtgccggg cgcaatggat gtgacggtgg agttcacctc catgtcgaag actttctcca tgcccggctg gcgcatgggc ttcgcggtcg gcaatgagcg tttgatcgcc gcgctgacgc gggtgaaatc ctatctcgat tatggcgcct ttacgccgat ccaggtggcg gcgacggcag cactcaatgg tgacggctcc gacattgcct atgtgcgcaa tgtctataag cagcgccgtg acgtgctggt tgaaagcttc ggccgcgctg gctgggatgt tccgccaccg gctgccacca tgtttgcctg ggttccgatc ccggaacggt tccgctcgct gggttcgctg gaattctcca aacttctggt ggagcaggcc gatgtggctg ttgcgccggg cgtcggtttt ggtgaacatg gcgacgatta tgtgcgcatc gcgctggtgg aaaacgagca tcgcattcgt caggccgcgc gcaatatcaa gcggttcttt tctgccaagg atgagaacct gcaaaatgtg attccgcttg aaggtcaccg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat