BrmeB.00629.a

Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases

CENTER ID: BrmeB.00629.a
ORGANISM: Brucella melitensis ATCC 23457
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.00629.a.B1.GE36929 Full length( BrmeB.00629.a ) 1 300
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel14466_2707

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGPLLLGVP TILNEGGFTA ENTYDIIERL GVTSLAGSPT AFRLLMAAGP ESAARVKGRL RVASSAGEPL NPEVIRWFDA CLGAPIHDHY GQTELGMVVN NHHGLEHPVR QGSAGYAMPG YRVAVLDEAG KEVGPNEPGV LAIDIDNSPL LWFTGYYKKD TPSISGGYYR TGDTVEFEPH GSISFIGRAD DVITSSGYRI GPFDVESALL EHPAVNEAAV VGVPDPQRTE IVKAFVILAP GFEGTPELAE ELALHVKKQL SAHAYPRQID FVAELPKTPS GKIQRFLLRK AEVEKQQQQN
NT Sequence
GTGACGGGTC CCCTGCTTCT GGGTGTGCCG ACGATCCTGA ACGAAGGCGG TTTCACCGCC GAAAACACCT ATGACATCAT CGAACGCCTC GGCGTGACGA GCCTTGCGGG CTCTCCGACT GCCTTCCGCC TGCTTATGGC CGCCGGGCCG GAATCTGCTG CCCGCGTCAA GGGCCGCTTG CGCGTGGCAT CCAGCGCGGG CGAGCCGCTG AACCCGGAAG TCATTCGCTG GTTCGATGCC TGTCTTGGCG CGCCGATCCA CGATCATTAC GGCCAGACGG AACTGGGCAT GGTGGTGAAC AACCATCATG GATTGGAACA TCCGGTGCGG CAGGGTTCGG CGGGCTATGC CATGCCGGGC TACAGGGTTG CGGTTCTGGA TGAAGCGGGC AAAGAAGTCG GCCCCAATGA ACCGGGCGTT CTGGCCATTG ATATCGACAA TTCGCCGCTT CTCTGGTTCA CCGGCTATTA TAAAAAGGAT ACGCCCTCCA TCTCCGGCGG ATATTATCGC ACCGGCGATA CGGTGGAATT CGAGCCGCAT GGGTCTATCA GCTTCATCGG CCGCGCCGAT GACGTCATCA CCTCCTCCGG CTATCGCATC GGGCCTTTCG ATGTGGAAAG CGCGCTTCTG GAACATCCTG CGGTCAACGA GGCTGCGGTG GTCGGCGTTC CCGATCCGCA ACGCACCGAA ATCGTGAAGG CCTTTGTCAT CCTGGCCCCC GGCTTCGAGG GTACGCCGGA ACTGGCGGAA GAACTGGCGC TTCACGTCAA GAAACAGCTT TCCGCCCATG CCTATCCGCG GCAGATCGAC TTTGTGGCGG AGCTTCCCAA GACGCCGAGC GGAAAAATAC AGCGCTTCCT CCTGCGCAAG GCGGAAGTCG AAAAGCAACA GCAACAGAAT
Details for BrmeB.00629.a.B1.GE36929
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV TGPLLLGVPT ILNEGGFTAE NTYDIIERLG VTSLAGSPTA FRLLMAAGPE SAARVKGRLR VASSAGEPLN PEVIRWFDAC LGAPIHDHYG QTELGMVVNN HHGLEHPVRQ GSAGYAMPGY RVAVLDEAGK EVGPNEPGVL AIDIDNSPLL WFTGYYKKDT PSISGGYYRT GDTVEFEPHG SISFIGRADD VITSSGYRIG PFDVESALLE HPAVNEAAVV GVPDPQRTEI VKAFVILAPG FEGTPELAEE LALHVKKQLS AHAYPRQIDF VAELPKTPSG KIQRFLLRKA EVEKQQQQN
Validated NT Sequence
tcttactcac ttaattctgt tgctgttgct tttcgacttc cgccttgcgc aggaggaagc gctgtatttt tccgctcggc gtcttgggaa gctccgccac aaagtcgatc tgccgcggat aggcatgggc ggaaagctgt ttcttgacgt gaagcgccag ttcttccgcc agttccggcg taccctcgaa gccgggggcc aggatgacaa aggccttcac gatttcggtg cgttgcggat cgggaacgcc gaccaccgca gcctcgttga ccgcaggatg ttccagaagc gcgctttcca catcgaaagg cccgatgcga tagccggagg aggtgatgac gtcatcggcg cggccgatga agctgataga cccatgcggc tcgaattcca ccgtatcgcc ggtgcgataa tatccgccgg agatggaggg cgtatccttt ttataatagc cggtgaacca gagaagcggc gaattgtcga tatcaatggc cagaacgccc ggttcattgg ggccgacttc tttgcccgct tcatccagaa ccgcaaccct gtagcccggc atggcatagc ccgccgaacc ctgccgcacc ggatgttcca atccatgatg gttgttcacc accatgccca gttccgtctg gccgtaatga tcgtggatcg gcgcgccaag acaggcatcg aaccagcgaa tgacttccgg gttcagcggc tcgcccgcgc tggatgccac gcgcaagcgg cccttgacgc gggcagcaga ttccggcccg gcggccataa gcaggcggaa ggcagtcgga gagcccgcaa ggctcgtcac gccgaggcgt tcgatgatgt cataggtgtt ttcggcggtg aaaccgcctt cgttcaggat cgtcggcaca cccagaagca ggggacccgt caccatatgg tggtggtggt ggtgagccat ggtat
Expected Protein Sequence
MAHHHHHHMT GPLLLGVPTI LNEGGFTAEN TYDIIERLGV TSLAGSPTAF RLLMAAGPES AARVKGRLRV ASSAGEPLNP EVIRWFDACL GAPIHDHYGQ TELGMVVNNH HGLEHPVRQG SAGYAMPGYR VAVLDEAGKE VGPNEPGVLA IDIDNSPLLW FTGYYKKDTP SISGGYYRTG DTVEFEPHGS ISFIGRADDV ITSSGYRIGP FDVESALLEH PAVNEAAVVG VPDPQRTEIV KAFVILAPGF EGTPELAEEL ALHVKKQLSA HAYPRQIDFV AELPKTPSGK IQRFLLRKAE VEKQQQQN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacgg gtcccctgct tctgggtgtg ccgacgatcc tgaacgaagg cggtttcacc gccgaaaaca cctatgacat catcgaacgc ctcggcgtga cgagccttgc gggctctccg actgccttcc gcctgcttat ggccgccggg ccggaatctg ctgcccgcgt caagggccgc ttgcgcgtgg catccagcgc gggcgagccg ctgaacccgg aagtcattcg ctggttcgat gcctgtcttg gcgcgccgat ccacgatcat tacggccaga cggaactggg catggtggtg aacaaccatc atggattgga acatccggtg cggcagggtt cggcgggcta tgccatgccg ggctacaggg ttgcggttct ggatgaagcg ggcaaagaag tcggccccaa tgaaccgggc gttctggcca ttgatatcga caattcgccg cttctctggt tcaccggcta ttataaaaag gatacgccct ccatctccgg cggatattat cgcaccggcg atacggtgga attcgagccg catgggtcta tcagcttcat cggccgcgcc gatgacgtca tcacctcctc cggctatcgc atcgggcctt tcgatgtgga aagcgcgctt ctggaacatc ctgcggtcaa cgaggctgcg gtggtcggcg ttcccgatcc gcaacgcacc gaaatcgtga aggcctttgt catcctggcc cccggcttcg agggtacgcc ggaactggcg gaagaactgg cgcttcacgt caagaaacag ctttccgccc atgcctatcc gcggcagatc gactttgtgg cggagcttcc caagacgccg agcggaaaaa tacagcgctt cctcctgcgc aaggcggaag tcgaaaagca acagcaacag aattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat