SSGCID: Target Page

BrmeB.00323.a

ZINC PROTEASE (EC 3.4.99.-)

CENTER ID:	BrmeB.00323.a
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.00323.a.B2.GE36883	Mature protein( BrmeB.00323.a )		30	455

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.00323.a.B2.PS01879	Mature protein( BrmeB.00323.a )		30	455

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_2476

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMNLSMSNFK RAFPLCGALH DARAVAGLLA ASFTMLIVCT LPARAIEIQE VVSPKGIHAW LVEDNSVPLV SMRFSFKGGA SQDPSGKEGI ANLMTGLFDE GAGDLDSDAF QERIDNLGAE MSFSASQDSV SGGVRMLAEN RDAATDLVAL AVNEPRFDQE AIDRIRQQIV AGIEAAQRNP STIASRKFAE VLYGNHPYAR DDEGTVKSLQ SISRDDLANF HRKNFARDKL TVGVVGAINA KDLGVMLDRI FGDLPASAEL VPVPDAKLAL GTTTSLNFDM PQTSISFVYP AIPRKDPEFF AAYLMNHILG GGFTSRLYNE VREKRGLAYS VSSSMVMHDH VSELMISTAT RPDKAQDSLK IIREQVAAMA NDGPTEEELA AAKSFLKGSY AVNNLDSSGA IANTLVSLQE AGLPSDYIDK RSELIDAVTL DQVKAIAWKL LQAEPAILIY GPAQS

NT Sequence

GTGATGAACC TGTCCATGTC GAATTTCAAA CGCGCTTTCC CCCTTTGCGG CGCCCTTCAT GACGCCCGTG CCGTCGCTGG CCTCTTGGCT GCGTCCTTCA CCATGCTGAT CGTCTGCACC CTGCCGGCGC GCGCCATCGA GATCCAGGAA GTGGTTTCGC CAAAAGGTAT CCACGCCTGG CTGGTGGAAG ACAATTCTGT TCCGCTCGTT TCCATGCGCT TCTCGTTCAA GGGCGGCGCC TCGCAAGATC CCTCCGGCAA GGAGGGGATT GCCAATCTGA TGACCGGCCT TTTCGATGAA GGTGCGGGCG ATCTCGATTC CGATGCCTTT CAGGAGCGGA TCGATAATCT GGGCGCGGAA ATGAGTTTTT CCGCTTCGCA GGATTCGGTT TCCGGCGGCG TCCGTATGCT GGCCGAAAAT CGTGATGCCG CGACCGATCT CGTGGCGCTT GCCGTCAACG AGCCGCGCTT CGATCAGGAA GCCATCGACC GCATTCGCCA GCAGATTGTT GCAGGCATTG AGGCCGCACA GCGCAATCCG TCTACGATTG CGTCGCGCAA GTTTGCCGAA GTGCTTTACG GCAACCACCC TTATGCGCGT GACGATGAGG GAACCGTGAA ATCCCTGCAA TCGATCAGCC GCGATGATCT TGCGAATTTC CATCGCAAGA ATTTCGCCCG CGACAAGCTG ACCGTTGGCG TTGTGGGTGC CATCAACGCC AAGGATCTGG GCGTCATGCT CGACAGGATT TTTGGCGACC TGCCCGCCTC GGCGGAACTG GTTCCGGTGC CGGATGCGAA GCTTGCGCTT GGCACCACCA CCAGCCTGAA TTTCGATATG CCGCAGACGT CGATCAGCTT CGTCTACCCG GCCATTCCGC GCAAAGACCC TGAGTTTTTT GCCGCCTATC TGATGAACCA TATTCTGGGC GGCGGTTTCA CCTCGCGTCT TTATAACGAA GTGCGTGAAA AGCGCGGCCT TGCCTATTCG GTGTCCTCGT CCATGGTCAT GCACGACCAT GTTTCCGAAC TGATGATTTC CACCGCCACC CGGCCTGACA AGGCCCAGGA CTCATTGAAA ATCATTCGTG AGCAGGTTGC CGCCATGGCC AATGACGGCC CGACCGAGGA AGAGCTTGCC GCAGCCAAGA GCTTTCTCAA AGGCTCCTAT GCGGTGAACA ATCTCGATTC GTCGGGAGCT ATTGCCAATA CGCTGGTGAG TTTGCAGGAA GCCGGGCTTC CAAGCGATTA TATCGACAAG CGTTCCGAAC TCATCGATGC TGTCACGCTG GATCAGGTCA AGGCCATTGC CTGGAAACTG CTTCAGGCCG AGCCTGCGAT CCTGATCTAT GGACCGGCGC AAAGT

Details for BrmeB.00323.a.B2.GE36883

HARVESTED ON:	5/1/2013
SEQUENCED ON:	5/8/2013
EXPECTED MW:	47kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

MLIVCTLPAR AIEIQEVVSP KGIHAWLVED NSVPLVSMRF SFKGGASQDP SGKEGIANLM TGLFDEGAGD LDSDAFQERI DNLGAEMSFS ASQDSVSGGV RMLAENRDAA TDLVALAVNE PRFDQEAIDR IRQQIVAGIE AAQRNPSTIA SRKFAEVLYG NHPYARDDEG TVKSLQSISR DDLANFHRKN FARDKLTVGV VGAINAKDLG VMLDRIFGDL PASAELVPVP DAKLALGTTT SLNFDMPQTS ISFVYPAIPR KDPEFFAAYL MNHILGGGFT SRLYNEVREK RGLAYSVSSS MVMHDHVSEL MISTATRPDK AQDSLKIIRE QVAAMANDGP TEEELAAAKS FLKGSYAVNN LDSSGAIANT LVSLQEAGLP SDYIDKRSEL IDAVTLDQVK AIAWKLLQAE PA

Validated NT Sequence

atcgcaggct cggcctgaag cagtttccag gcaatggcct tgacctgatc cagcgtgaca gcatcgatga gttcggaacg cttgtcgata taatcgcttg gaagcccggc ttcctgcaaa ctcaccagcg tattggcaat agctcccgac gaatcgagat tgttcaccgc ataggagcct ttgagaaagc tcttggctgc ggcaagctct tcctcggtcg ggccgtcatt ggccatggcg gcaacctgct cacgaatgat tttcaatgag tcctgggcct tgtcaggccg ggtggcggtg gaaatcatca gttcggaaac atggtcgtgc atgaccatgg acgaggacac cgaataggca aggccgcgct tttcacgcac ttcgttataa agacgcgagg tgaaaccgcc gcccagaata tggttcatca gataggcggc aaaaaactca gggtctttgc gcggaatggc cgggtagacg aagctgatcg acgtctgcgg catatcgaaa ttcaggctgg tggtggtgcc aagcgcaagc ttcgcatccg gcaccggaac cagttccgcc gaggcgggca ggtcgccaaa aatcctgtcg agcatgacgc ccagatcctt ggcgttgatg gcacccacaa cgccaacggt cagcttgtcg cgggcgaaat tcttgcgatg gaaattcgca agatcatcgc ggctgatcga ttgcagggat ttcacggttc cctcatcgtc acgcgcataa gggtggttgc cgtaaagcac ttcggcaaac ttgcgcgacg caatcgtaga cggattgcgc tgtgcggcct caatgcctgc aacaatctgc tggcgaatgc ggtcgatggc ttcctgatcg aagcgcggct cgttgacggc aagcgccacg agatcggtcg cggcatcacg attttcggcc agcatacgga cgccgccgga aaccgaatcc tgcgaagcgg aaaaactcat ttccgcgccc agattatcga tccgctcctg aaaggcatcg gaatcgagat cgcccgcacc ttcatcgaaa aggccggtca tcagattggc aatcccctcc ttgccggagg gatcttgcga ggcgccgccc ttgaacgaga agcgcatgga aacgagcgga acagaattgt cttccaccag ccaggcgtgg ataccttttg gcgaaaccac ttcctggatc tcgatggcgc gcgccggcag ggtgcagacg atcagcatgg tgaaggac

Expected Protein Sequence

MAHHHHHHAA SFTMLIVCTL PARAIEIQEV VSPKGIHAWL VEDNSVPLVS MRFSFKGGAS QDPSGKEGIA NLMTGLFDEG AGDLDSDAFQ ERIDNLGAEM SFSASQDSVS GGVRMLAENR DAATDLVALA VNEPRFDQEA IDRIRQQIVA GIEAAQRNPS TIASRKFAEV LYGNHPYARD DEGTVKSLQS ISRDDLANFH RKNFARDKLT VGVVGAINAK DLGVMLDRIF GDLPASAELV PVPDAKLALG TTTSLNFDMP QTSISFVYPA IPRKDPEFFA AYLMNHILGG GFTSRLYNEV REKRGLAYSV SSSMVMHDHV SELMISTATR PDKAQDSLKI IREQVAAMAN DGPTEEELAA AKSFLKGSYA VNNLDSSGAI ANTLVSLQEA GLPSDYIDKR SELIDAVTLD QVKAIAWKLL QAEPAILIYG PAQS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctgcgt ccttcaccat gctgatcgtc tgcaccctgc cggcgcgcgc catcgagatc caggaagtgg tttcgccaaa aggtatccac gcctggctgg tggaagacaa ttctgttccg ctcgtttcca tgcgcttctc gttcaagggc ggcgcctcgc aagatccctc cggcaaggag gggattgcca atctgatgac cggccttttc gatgaaggtg cgggcgatct cgattccgat gcctttcagg agcggatcga taatctgggc gcggaaatga gtttttccgc ttcgcaggat tcggtttccg gcggcgtccg tatgctggcc gaaaatcgtg atgccgcgac cgatctcgtg gcgcttgccg tcaacgagcc gcgcttcgat caggaagcca tcgaccgcat tcgccagcag attgttgcag gcattgaggc cgcacagcgc aatccgtcta cgattgcgtc gcgcaagttt gccgaagtgc tttacggcaa ccacccttat gcgcgtgacg atgagggaac cgtgaaatcc ctgcaatcga tcagccgcga tgatcttgcg aatttccatc gcaagaattt cgcccgcgac aagctgaccg ttggcgttgt gggtgccatc aacgccaagg atctgggcgt catgctcgac aggatttttg gcgacctgcc cgcctcggcg gaactggttc cggtgccgga tgcgaagctt gcgcttggca ccaccaccag cctgaatttc gatatgccgc agacgtcgat cagcttcgtc tacccggcca ttccgcgcaa agaccctgag ttttttgccg cctatctgat gaaccatatt ctgggcggcg gtttcacctc gcgtctttat aacgaagtgc gtgaaaagcg cggccttgcc tattcggtgt cctcgtccat ggtcatgcac gaccatgttt ccgaactgat gatttccacc gccacccggc ctgacaaggc ccaggactca ttgaaaatca ttcgtgagca ggttgccgcc atggccaatg acggcccgac cgaggaagag cttgccgcag ccaagagctt tctcaaaggc tcctatgcgg tgaacaatct cgattcgtcg ggagctattg ccaatacgct ggtgagtttg caggaagccg ggcttccaag cgattatatc gacaagcgtt ccgaactcat cgatgctgtc acgctggatc aggtcaaggc cattgcctgg aaactgcttc aggccgagcc tgcgatcctg atctatggac cggcgcaaag ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BrmeB.00323.a.B2.PS01879

PURIFICATION DATe:	8/8/2013
CONCENTRATION:	25mg/ml
OBSERVED MW:	49kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCTGCGT CCTTCACCAT GCTGATCGTC TGCACCCTGC CGGCGCGCGC CATCGAGATC CAGGAAGTGG TTTCGCCAAA AGGTATCCAC GCCTGGCTGG TGGAAGACAA TTCTGTTCCG CTCGTTTCCA TGCGCTTCTC GTTCAAGGGC GGCGCCTCGC AAGATCCCTC CGGCAAGGAG GGGATTGCCA ATCTGATGAC CGGCCTTTTC GATGAAGGTG CGGGCGATCT CGATTCCGAT GCCTTTCAGG AGCGGATCGA TAATCTGGGC GCGGAAATGA GTTTTTCCGC TTCGCAGGAT TCGGTTTCCG GCGGCGTCCG TATGCTGGCC GAAAATCGTG ATGCCGCGAC CGATCTCGTG GCGCTTGCCG TCAACGAGCC GCGCTTCGAT CAGGAAGCCA TCGACCGCAT TCGCCAGCAG ATTGTTGCAG GCATTGAGGC CGCACAGCGC AATCCGTCTA CGATTGCGTC GCGCAAGTTT GCCGAAGTGC TTTACGGCAA CCACCCTTAT GCGCGTGACG ATGAGGGAAC CGTGAAATCC CTGCAATCGA TCAGCCGCGA TGATCTTGCG AATTTCCATC GCAAGAATTT CGCCCGCGAC AAGCTGACCG TTGGCGTTGT GGGTGCCATC AACGCCAAGG ATCTGGGCGT CATGCTCGAC AGGATTTTTG GCGACCTGCC CGCCTCGGCG GAACTGGTTC CGGTGCCGGA TGCGAAGCTT GCGCTTGGCA CCACCACCAG CCTGAATTTC GATATGCCGC AGACGTCGAT CAGCTTCGTC TACCCGGCCA TTCCGCGCAA AGACCCTGAG TTTTTTGCCG CCTATCTGAT GAACCATATT CTGGGCGGCG GTTTCACCTC GCGTCTTTAT AACGAAGTGC GTGAAAAGCG CGGCCTTGCC TATTCGGTGT CCTCGTCCAT GGTCATGCAC GACCATGTTT CCGAACTGAT GATTTCCACC GCCACCCGGC CTGACAAGGC CCAGGACTCA TTGAAAATCA TTCGTGAGCA GGTTGCCGCC ATGGCCAATG ACGGCCCGAC CGAGGAAGAG CTTGCCGCAG CCAAGAGCTT TCTCAAAGGC TCCTATGCGG TGAACAATCT CGATTCGTCG GGAGCTATTG CCAATACGCT GGTGAGTTTG CAGGAAGCCG GGCTTCCAAG CGATTATATC GACAAGCGTT CCGAACTCAT CGATGCTGTC ACGCTGGATC AGGTCAAGGC CATTGCCTGG AAACTGCTTC AGGCCGAGCC TGCGATCCTG ATCTATGGAC CGGCGCAAAG TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT