BrmeB.00143.f

D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30)

CENTER ID: BrmeB.00143.f
ORGANISM: Brucella melitensis ATCC 23457
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.00143.f.B1.GE36875 Full length( BrmeB.00143.f ) 1 263
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.00143.f.B1.PW37377 Full length( BrmeB.00143.f ) 1 263

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel14466_1848
BV-BRC: fig|546272.3.peg.1848
RefSeq: YP_002733459.1
UniProt: C0RF33

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGVSARKTA IVTGSNSGIG LGIAHALAAA GHNVVINSFT DRDEDHALAR MLAEEHGANV IYIAADMSKA EDCRNLIAKT VKVFGNSDIL VNNAGIQHVA PVEEFPPEQW DRIIAINLTS AFHTSAAAIP HMRKAGWGRI INIASAHGLV ASPFKSAYVA AKHGIVGFTK TLALELATAK ITANSICPGY VLTPLVEAQI PDQMKAHHMD RETVIRDVML DKQPTKEFVT TAQIGAVATF LASDAAAQIN GAAISVDGGW TAE
NT Sequence
ATGACCGGCG TATCCGCCCG CAAAACTGCA ATTGTTACCG GATCAAATTC AGGGATCGGC CTCGGAATCG CCCATGCACT GGCAGCCGCG GGGCATAACG TCGTAATAAA CTCCTTCACC GACCGGGACG AAGACCATGC GCTTGCCCGG ATGCTTGCCG AAGAACATGG TGCGAATGTC ATCTATATTG CCGCGGATAT GTCGAAAGCC GAAGATTGCC GCAATCTCAT TGCCAAAACC GTCAAGGTTT TCGGCAACTC CGATATTCTG GTCAATAATG CGGGCATCCA GCATGTCGCC CCGGTGGAAG AGTTTCCTCC CGAGCAATGG GACCGCATCA TCGCAATCAA CCTGACATCG GCCTTCCACA CTTCTGCTGC TGCCATCCCC CATATGCGAA AGGCGGGCTG GGGCCGTATC ATCAATATTG CCTCGGCGCA TGGTCTCGTG GCTTCGCCGT TCAAATCGGC ATATGTCGCC GCCAAGCACG GCATTGTCGG CTTCACCAAG ACGCTGGCGC TGGAGCTTGC CACCGCAAAA ATCACCGCCA ATTCCATCTG TCCCGGCTAT GTGCTGACCC CTCTGGTGGA AGCGCAGATA CCCGACCAGA TGAAAGCCCA CCACATGGAC CGTGAAACCG TGATCCGCGA CGTGATGCTG GATAAACAGC CGACGAAGGA GTTTGTCACC ACCGCGCAAA TCGGGGCCGT TGCCACTTTC CTCGCAAGCG ATGCGGCAGC GCAGATCAAC GGTGCAGCCA TTTCCGTCGA CGGCGGCTGG ACGGCAGAA
Details for BrmeB.00143.f.B1.GE36875
HARVESTED ON: 5/1/2013
SEQUENCED ON: 5/8/2013
EXPECTED MW: 29kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT GVSARKTAIV TGSNSGIGLG IAHALAAAGH NVVINSFTDR DEDHALARML AEEHGANVIY IAADMSKAED CRNLIAKTVK VFGNSDILVN NAGIQHVAPV EEFPPEQWDR IIAINLTSAF HTSAAAIPHM RKAGWGRIIN IASAHGLVAS PFKSAYVAAK HGIVGFTKTL ALELATAKIT ANSICPGYVL TPLVEAQIPD QMKAHHMDRE TVIRDVMLDK QPTKEFVTTA QIGAVATFLA SDAAAQINGA AISVDGGWTA E
Validated NT Sequence
atcttactca cttattctgc cgtccagccg ccgtcgacgg aaatggctgc accgttgatc tgcgctgccg catcgcttgc gaggaaagtg gcaacggccc cgatttgcgc ggtggtgaca aactccttcg tcggctgttt atccagcatc acgtcgcgga tcacggtttc acggtccatg tggtgggctt tcatctggtc gggtatctgc gcttccacca gaggggtcag cacatagccg ggacagatgg aattggcggt gatttttgcg gtggcaagct ccagcgccag cgtcttggtg aagccgacaa tgccgtgctt ggcggcgaca tatgccgatt tgaacggcga agccacgaga ccatgcgccg aggcaatatt gatgatacgg ccccagcccg cctttcgcat atgggggatg gcagcagcag aagtgtggaa ggccgatgtc aggttgattg cgatgatgcg gtcccattgc tcgggaggaa actcttccac cggggcgaca tgctggatgc ccgcattatt gaccagaata tcggagttgc cgaaaacctt gacggttttg gcaatgagat tgcggcaatc ttcggctttc gacatatccg cggcaatata gatgacattc gcaccatgtt cttcggcaag catccgggca agcgcatggt cttcgtcccg gtcggtgaag gagtttatta cgacgttatg ccccgcggct gccagtgcat gggcgattcc gaggccgatc cctgaatttg atccggtaac aattgcagtt ttgcgggcgg atacgccggt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMT GVSARKTAIV TGSNSGIGLG IAHALAAAGH NVVINSFTDR DEDHALARML AEEHGANVIY IAADMSKAED CRNLIAKTVK VFGNSDILVN NAGIQHVAPV EEFPPEQWDR IIAINLTSAF HTSAAAIPHM RKAGWGRIIN IASAHGLVAS PFKSAYVAAK HGIVGFTKTL ALELATAKIT ANSICPGYVL TPLVEAQIPD QMKAHHMDRE TVIRDVMLDK QPTKEFVTTA QIGAVATFLA SDAAAQINGA AISVDGGWTA E
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg gcgtatccgc ccgcaaaact gcaattgtta ccggatcaaa ttcagggatc ggcctcggaa tcgcccatgc actggcagcc gcggggcata acgtcgtaat aaactccttc accgaccggg acgaagacca tgcgcttgcc cggatgcttg ccgaagaaca tggtgcgaat gtcatctata ttgccgcgga tatgtcgaaa gccgaagatt gccgcaatct cattgccaaa accgtcaagg ttttcggcaa ctccgatatt ctggtcaata atgcgggcat ccagcatgtc gccccggtgg aagagtttcc tcccgagcaa tgggaccgca tcatcgcaat caacctgaca tcggccttcc acacttctgc tgctgccatc ccccatatgc gaaaggcggg ctggggccgt atcatcaata ttgcctcggc gcatggtctc gtggcttcgc cgttcaaatc ggcatatgtc gccgccaagc acggcattgt cggcttcacc aagacgctgg cgctggagct tgccaccgca aaaatcaccg ccaattccat ctgtcccggc tatgtgctga cccctctggt ggaagcgcag atacccgacc agatgaaagc ccaccacatg gaccgtgaaa ccgtgatccg cgacgtgatg ctggataaac agccgacgaa ggagtttgtc accaccgcgc aaatcggggc cgttgccact ttcctcgcaa gcgatgcggc agcgcagatc aacggtgcag ccatttccgt cgacggcggc tggacggcag aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BrmeB.00143.f.B1.PW37377
PURIFICATION DATe: 10/14/2013
CONCENTRATION: 22.2mg/ml
OBSERVED MW: 29kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT GVSARKTAIV TGSNSGIGLG IAHALAAAGH NVVINSFTDR DEDHALARML AEEHGANVIY IAADMSKAED CRNLIAKTVK VFGNSDILVN NAGIQHVAPV EEFPPEQWDR IIAINLTSAF HTSAAAIPHM RKAGWGRIIN IASAHGLVAS PFKSAYVAAK HGIVGFTKTL ALELATAKIT ANSICPGYVL TPLVEAQIPD QMKAHHMDRE TVIRDVMLDK QPTKEFVTTA QIGAVATFLA SDAAAQINGA AISVDGGWTA E
Validated NT Sequence
atcttactca cttattctgc cgtccagccg ccgtcgacgg aaatggctgc accgttgatc tgcgctgccg catcgcttgc gaggaaagtg gcaacggccc cgatttgcgc ggtggtgaca aactccttcg tcggctgttt atccagcatc acgtcgcgga tcacggtttc acggtccatg tggtgggctt tcatctggtc gggtatctgc gcttccacca gaggggtcag cacatagccg ggacagatgg aattggcggt gatttttgcg gtggcaagct ccagcgccag cgtcttggtg aagccgacaa tgccgtgctt ggcggcgaca tatgccgatt tgaacggcga agccacgaga ccatgcgccg aggcaatatt gatgatacgg ccccagcccg cctttcgcat atgggggatg gcagcagcag aagtgtggaa ggccgatgtc aggttgattg cgatgatgcg gtcccattgc tcgggaggaa actcttccac cggggcgaca tgctggatgc ccgcattatt gaccagaata tcggagttgc cgaaaacctt gacggttttg gcaatgagat tgcggcaatc ttcggctttc gacatatccg cggcaatata gatgacattc gcaccatgtt cttcggcaag catccgggca agcgcatggt cttcgtcccg gtcggtgaag gagtttatta cgacgttatg ccccgcggct gccagtgcat gggcgattcc gaggccgatc cctgaatttg atccggtaac aattgcagtt ttgcgggcgg atacgccggt catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMT GVSARKTAIV TGSNSGIGLG IAHALAAAGH NVVINSFTDR DEDHALARML AEEHGANVIY IAADMSKAED CRNLIAKTVK VFGNSDILVN NAGIQHVAPV EEFPPEQWDR IIAINLTSAF HTSAAAIPHM RKAGWGRIIN IASAHGLVAS PFKSAYVAAK HGIVGFTKTL ALELATAKIT ANSICPGYVL TPLVEAQIPD QMKAHHMDRE TVIRDVMLDK QPTKEFVTTA QIGAVATFLA SDAAAQINGA AISVDGGWTA E
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCG GCGTATCCGC CCGCAAAACT GCAATTGTTA CCGGATCAAA TTCAGGGATC GGCCTCGGAA TCGCCCATGC ACTGGCAGCC GCGGGGCATA ACGTCGTAAT AAACTCCTTC ACCGACCGGG ACGAAGACCA TGCGCTTGCC CGGATGCTTG CCGAAGAACA TGGTGCGAAT GTCATCTATA TTGCCGCGGA TATGTCGAAA GCCGAAGATT GCCGCAATCT CATTGCCAAA ACCGTCAAGG TTTTCGGCAA CTCCGATATT CTGGTCAATA ATGCGGGCAT CCAGCATGTC GCCCCGGTGG AAGAGTTTCC TCCCGAGCAA TGGGACCGCA TCATCGCAAT CAACCTGACA TCGGCCTTCC ACACTTCTGC TGCTGCCATC CCCCATATGC GAAAGGCGGG CTGGGGCCGT ATCATCAATA TTGCCTCGGC GCATGGTCTC GTGGCTTCGC CGTTCAAATC GGCATATGTC GCCGCCAAGC ACGGCATTGT CGGCTTCACC AAGACGCTGG CGCTGGAGCT TGCCACCGCA AAAATCACCG CCAATTCCAT CTGTCCCGGC TATGTGCTGA CCCCTCTGGT GGAAGCGCAG ATACCCGACC AGATGAAAGC CCACCACATG GACCGTGAAA CCGTGATCCG CGACGTGATG CTGGATAAAC AGCCGACGAA GGAGTTTGTC ACCACCGCGC AAATCGGGGC CGTTGCCACT TTCCTCGCAA GCGATGCGGC AGCGCAGATC AACGGTGCAG CCATTTCCGT CGACGGCGGC TGGACGGCAG AATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT