BrmeB.00143.c
SHORT-CHAIN DEHYDROGENASE
| CENTER ID: | BrmeB.00143.c |
| ORGANISM: | Brucella melitensis ATCC 23457 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeB.00143.c.B1.GE36872 | Full length( BrmeB.00143.c ) | 1 | 269 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeB.00143.c.B1.PS01906 | Full length( BrmeB.00143.c ) | 1 | 269 |
Structures
4NIM
DEPOSITED: 11/6/2013
DETERMINATION: XRay
CLONE: BrmeB.00143.c.B1.GE36872
PROTEIN: BrmeB.00143.c.B1.PS01906
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|546272.3.peg.1411 |
| RefSeq: | YP_002733057.1 |
| UniProt: | C0RJU5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGGQLKGKIA VITGSTQGLG AATARLFAER GAQGLVICGR SAEKGRAQAA GLEELGAKAV FVQVDLENVE DCRRIVAEAD RAFGRLDILV NAAGLTDRGT ILDTSPELFD RLFAVNTRAP FFLIQEAIKL FRRDRVEGAI VNVSSMSSMG GQPFIAAYCA SKGALDTLTR NVAYSVLRNR IRVNSLNIGW MASDGEDRIQ REYHGAPANW LEEAVKTQPF GRLLDPAKVA RAIAFLASEE SGLMTGAIVN FDQSVWGAYD GSPHPEKPL
NT Sequence
ATGGGCGGAC AGTTGAAGGG TAAGATTGCA GTCATTACAG GCAGCACGCA GGGGCTGGGC GCGGCCACCG CCCGGCTGTT TGCCGAACGG GGCGCGCAAG GCCTTGTGAT CTGCGGGCGT TCTGCCGAAA AGGGCAGGGC GCAGGCTGCC GGGCTTGAAG AACTGGGCGC AAAAGCGGTT TTCGTGCAGG TGGACCTTGA AAATGTGGAA GACTGCCGCC GCATCGTTGC AGAAGCTGAC CGGGCATTCG GCAGGCTTGA TATTCTGGTG AATGCGGCGG GACTGACCGA TCGCGGCACT ATTCTGGATA CATCACCGGA ACTGTTCGAC CGGCTCTTTG CCGTGAACAC GCGCGCGCCG TTTTTCCTTA TTCAGGAAGC GATCAAGCTT TTCCGCCGTG ACAGGGTGGA GGGTGCGATT GTGAATGTCT CCTCCATGTC GTCCATGGGA GGCCAGCCTT TTATTGCAGC CTATTGCGCC TCCAAGGGCG CGCTCGACAC GCTGACGCGC AATGTTGCCT ATTCGGTTCT TCGCAACCGC ATTCGGGTCA ACAGCCTCAA TATCGGCTGG ATGGCATCCG ATGGCGAGGA TCGTATCCAG CGTGAATATC ATGGTGCACC GGCAAACTGG CTGGAGGAAG CCGTAAAGAC GCAACCTTTC GGGCGGCTTC TCGACCCGGC GAAAGTGGCG CGCGCCATTG CTTTTCTGGC CTCGGAGGAA TCAGGACTGA TGACGGGTGC TATCGTCAAT TTCGACCAGT CGGTCTGGGG CGCTTATGAC GGTTCTCCGC ATCCTGAGAA GCCGCTT
Details for BrmeB.00143.c.B1.GE36872
| HARVESTED ON: | 5/1/2013 |
| SEQUENCED ON: | 5/8/2013 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG GQLKGKIAVI TGSTQGLGAA TARLFAERGA QGLVICGRSA EKGRAQAAGL EELGAKAVFV QVDLENVEDC RRIVAEADRA FGRLDILVNA AGLTDRGTIL DTSPELFDRL FAVNTRAPFF LIQEAIKLFR RDRVEGAIVN VSSMSSMGGQ PFIAAYCASK GALDTLTRNV AYSVLRNRIR VNSLNIGWMA SDGEDRIQRE YHGAPANWLE EAVKTQPFGR LLDPAKVARA IAFLASEESG LMTGAIVNFD QSVWGAYDGS PHPEKPL
Validated NT Sequence
tcttactcac ttaaagcggc ttctcaggat gcggagaacc gtcataagcg ccccagaccg actggtcgaa attgacgata gcacccgtca tcagtcctga ttcctccgag gccagaaaag caatggcgcg cgccactttc gccgggtcga gaagccgccc gaaaggttgc gtctttacgg cttcctccag ccagtttgcc ggtgcaccat gatattcacg ctggatacga tcctcgccat cggatgccat ccagccgata ttgaggctgt tgacccgaat gcggttgcga agaaccgaat aggcaacatt gcgcgtcagc gtgtcgagcg cgcccttgga ggcgcaatag gctgcaataa aaggctggcc tcccatggac gacatggagg agacattcac aatcgcaccc tccaccctgt cacggcggaa aagcttgatc gcttcctgaa taaggaaaaa cggcgcgcgc gtgttcacgg caaagagccg gtcgaacagt tccggtgatg tatccagaat agtgccgcga tcggtcagtc ccgccgcatt caccagaata tcaagcctgc cgaatgcccg gtcagcttct gcaacgatgc ggcggcagtc ttccacattt tcaaggtcca cctgcacgaa aaccgctttt gcgcccagtt cttcaagccc ggcagcctgc gccctgccct tttcggcaga acgcccgcag atcacaaggc cttgcgcgcc ccgttcggca aacagccggg cggtggccgc gcccagcccc tgcgtgctgc ctgtaatgac tgcaatctta cccttcaact gtccgcccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMG GQLKGKIAVI TGSTQGLGAA TARLFAERGA QGLVICGRSA EKGRAQAAGL EELGAKAVFV QVDLENVEDC RRIVAEADRA FGRLDILVNA AGLTDRGTIL DTSPELFDRL FAVNTRAPFF LIQEAIKLFR RDRVEGAIVN VSSMSSMGGQ PFIAAYCASK GALDTLTRNV AYSVLRNRIR VNSLNIGWMA SDGEDRIQRE YHGAPANWLE EAVKTQPFGR LLDPAKVARA IAFLASEESG LMTGAIVNFD QSVWGAYDGS PHPEKPL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcg gacagttgaa gggtaagatt gcagtcatta caggcagcac gcaggggctg ggcgcggcca ccgcccggct gtttgccgaa cggggcgcgc aaggccttgt gatctgcggg cgttctgccg aaaagggcag ggcgcaggct gccgggcttg aagaactggg cgcaaaagcg gttttcgtgc aggtggacct tgaaaatgtg gaagactgcc gccgcatcgt tgcagaagct gaccgggcat tcggcaggct tgatattctg gtgaatgcgg cgggactgac cgatcgcggc actattctgg atacatcacc ggaactgttc gaccggctct ttgccgtgaa cacgcgcgcg ccgtttttcc ttattcagga agcgatcaag cttttccgcc gtgacagggt ggagggtgcg attgtgaatg tctcctccat gtcgtccatg ggaggccagc cttttattgc agcctattgc gcctccaagg gcgcgctcga cacgctgacg cgcaatgttg cctattcggt tcttcgcaac cgcattcggg tcaacagcct caatatcggc tggatggcat ccgatggcga ggatcgtatc cagcgtgaat atcatggtgc accggcaaac tggctggagg aagccgtaaa gacgcaacct ttcgggcggc ttctcgaccc ggcgaaagtg gcgcgcgcca ttgcttttct ggcctcggag gaatcaggac tgatgacggg tgctatcgtc aatttcgacc agtcggtctg gggcgcttat gacggttctc cgcatcctga gaagccgctt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BrmeB.00143.c.B1.PS01906
| PURIFICATION DATe: | 9/19/2013 |
| CONCENTRATION: | 15.36mg/ml |
| OBSERVED MW: | 30kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG GQLKGKIAVI TGSTQGLGAA TARLFAERGA QGLVICGRSA EKGRAQAAGL EELGAKAVFV QVDLENVEDC RRIVAEADRA FGRLDILVNA AGLTDRGTIL DTSPELFDRL FAVNTRAPFF LIQEAIKLFR RDRVEGAIVN VSSMSSMGGQ PFIAAYCASK GALDTLTRNV AYSVLRNRIR VNSLNIGWMA SDGEDRIQRE YHGAPANWLE EAVKTQPFGR LLDPAKVARA IAFLASEESG LMTGAIVNFD QSVWGAYDGS PHPEKPL
Validated NT Sequence
tcttactcac ttaaagcggc ttctcaggat gcggagaacc gtcataagcg ccccagaccg actggtcgaa attgacgata gcacccgtca tcagtcctga ttcctccgag gccagaaaag caatggcgcg cgccactttc gccgggtcga gaagccgccc gaaaggttgc gtctttacgg cttcctccag ccagtttgcc ggtgcaccat gatattcacg ctggatacga tcctcgccat cggatgccat ccagccgata ttgaggctgt tgacccgaat gcggttgcga agaaccgaat aggcaacatt gcgcgtcagc gtgtcgagcg cgcccttgga ggcgcaatag gctgcaataa aaggctggcc tcccatggac gacatggagg agacattcac aatcgcaccc tccaccctgt cacggcggaa aagcttgatc gcttcctgaa taaggaaaaa cggcgcgcgc gtgttcacgg caaagagccg gtcgaacagt tccggtgatg tatccagaat agtgccgcga tcggtcagtc ccgccgcatt caccagaata tcaagcctgc cgaatgcccg gtcagcttct gcaacgatgc ggcggcagtc ttccacattt tcaaggtcca cctgcacgaa aaccgctttt gcgcccagtt cttcaagccc ggcagcctgc gccctgccct tttcggcaga acgcccgcag atcacaaggc cttgcgcgcc ccgttcggca aacagccggg cggtggccgc gcccagcccc tgcgtgctgc ctgtaatgac tgcaatctta cccttcaact gtccgcccat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMG GQLKGKIAVI TGSTQGLGAA TARLFAERGA QGLVICGRSA EKGRAQAAGL EELGAKAVFV QVDLENVEDC RRIVAEADRA FGRLDILVNA AGLTDRGTIL DTSPELFDRL FAVNTRAPFF LIQEAIKLFR RDRVEGAIVN VSSMSSMGGQ PFIAAYCASK GALDTLTRNV AYSVLRNRIR VNSLNIGWMA SDGEDRIQRE YHGAPANWLE EAVKTQPFGR LLDPAKVARA IAFLASEESG LMTGAIVNFD QSVWGAYDGS PHPEKPL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGCG GACAGTTGAA GGGTAAGATT GCAGTCATTA CAGGCAGCAC GCAGGGGCTG GGCGCGGCCA CCGCCCGGCT GTTTGCCGAA CGGGGCGCGC AAGGCCTTGT GATCTGCGGG CGTTCTGCCG AAAAGGGCAG GGCGCAGGCT GCCGGGCTTG AAGAACTGGG CGCAAAAGCG GTTTTCGTGC AGGTGGACCT TGAAAATGTG GAAGACTGCC GCCGCATCGT TGCAGAAGCT GACCGGGCAT TCGGCAGGCT TGATATTCTG GTGAATGCGG CGGGACTGAC CGATCGCGGC ACTATTCTGG ATACATCACC GGAACTGTTC GACCGGCTCT TTGCCGTGAA CACGCGCGCG CCGTTTTTCC TTATTCAGGA AGCGATCAAG CTTTTCCGCC GTGACAGGGT GGAGGGTGCG ATTGTGAATG TCTCCTCCAT GTCGTCCATG GGAGGCCAGC CTTTTATTGC AGCCTATTGC GCCTCCAAGG GCGCGCTCGA CACGCTGACG CGCAATGTTG CCTATTCGGT TCTTCGCAAC CGCATTCGGG TCAACAGCCT CAATATCGGC TGGATGGCAT CCGATGGCGA GGATCGTATC CAGCGTGAAT ATCATGGTGC ACCGGCAAAC TGGCTGGAGG AAGCCGTAAA GACGCAACCT TTCGGGCGGC TTCTCGACCC GGCGAAAGTG GCGCGCGCCA TTGCTTTTCT GGCCTCGGAG GAATCAGGAC TGATGACGGG TGCTATCGTC AATTTCGACC AGTCGGTCTG GGGCGCTTAT GACGGTTCTC CGCATCCTGA GAAGCCGCTT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT