BrmeB.00111.a

glutamine synthetase family protein

CENTER ID: BrmeB.00111.a
ORGANISM: Brucella melitensis ATCC 23457
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.00111.a.B1.GE36869 Full length( BrmeB.00111.a ) 1 455
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.00111.a.B1.PW37375 Full length( BrmeB.00111.a ) 1 455

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel14466_2991
BV-BRC: fig|546272.3.peg.2991
RefSeq: YP_002734474.1
UniProt: C0RLN7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGQLTFDEL KKAVAEGRID TVLACIVDMQ GRLIGKRFYG QFFVESGYDE THGCNYLLAD DIDMEPVPGY EAAGWDKGYG DFVMKPDLST LRLAPWLEKT AIVLCDVLDH HHDDLSHSPR AVLKKQVQRL HERGYRAYFA SELEFYIFDE TYKSARAKRW HEMETASPYV QGYVIHLTTR EEPVLRAMRN HLADAGIPVE NSKGEWGPGQ QELNVRYCKA LEMADRHVIM KNAMKEIAEA HGKCITFMAK YDYARAGSSS HVHNSIWSAD GKEPLFFDPK APYTMTPLMR SWVAGQIKYA TDYTYFLAPY INSYKRFQAG TFTPTKIMWS QDNRTAGFRL CGEGTKGIRI ECRIGGADIN PYLAFAALIA AGLKGVDEKL ELDEPFVGDA YSAVRLKEIP YTLREAAAAL KGSAFLKEAF GEDVVNHYTH TAHWEQIEYD RRVTDWELYR GFERY
NT Sequence
ATGGCCGGAC AGTTGACTTT TGACGAATTG AAGAAGGCAG TCGCGGAAGG CAGGATCGAC ACGGTGCTGG CCTGCATCGT GGACATGCAG GGCCGCCTGA TTGGAAAGCG CTTCTACGGA CAGTTTTTCG TGGAATCCGG TTATGACGAA ACCCACGGCT GCAATTATCT TCTGGCCGAC GATATCGATA TGGAACCCGT ACCGGGCTAT GAGGCGGCGG GTTGGGACAA AGGCTATGGC GATTTCGTCA TGAAGCCGGA TCTCTCCACG CTGCGCCTTG CGCCGTGGCT GGAAAAGACG GCCATTGTGC TGTGCGATGT ACTGGACCAC CATCATGACG ATCTTTCCCA TTCGCCGCGC GCCGTCCTGA AAAAACAGGT GCAGCGCCTG CACGAGCGCG GTTATCGCGC TTATTTCGCA TCCGAGCTTG AATTCTACAT TTTCGATGAA ACCTATAAAT CGGCGCGGGC AAAACGCTGG CATGAGATGG AAACCGCCTC GCCTTACGTG CAGGGTTATG TGATCCACCT CACCACCAGG GAGGAGCCGG TCCTGCGCGC CATGCGCAAC CATCTGGCCG ATGCGGGCAT TCCGGTGGAA AATTCAAAAG GCGAATGGGG GCCGGGCCAG CAGGAGCTGA ACGTGCGCTA TTGCAAGGCG CTTGAAATGG CCGATCGCCA CGTCATCATG AAAAACGCGA TGAAGGAGAT TGCCGAGGCG CATGGCAAGT GCATCACCTT CATGGCGAAG TATGATTATG CAAGGGCTGG CAGTTCGAGC CATGTGCACA ATTCCATCTG GAGTGCAGAT GGCAAGGAAC CTCTGTTCTT CGATCCCAAA GCCCCCTACA CCATGACACC GCTGATGCGC TCATGGGTGG CCGGCCAGAT CAAATATGCG ACCGATTACA CCTATTTTCT GGCGCCTTAC ATCAATTCCT ACAAGCGGTT TCAGGCGGGC ACCTTCACCC CCACCAAGAT CATGTGGAGC CAGGACAACC GCACAGCCGG TTTTCGCCTT TGCGGCGAAG GTACCAAGGG CATTCGCATC GAATGCCGCA TCGGCGGCGC GGATATCAAT CCCTATCTGG CCTTTGCAGC GCTCATCGCC GCCGGCCTGA AAGGCGTGGA TGAAAAGCTG GAACTGGACG AACCTTTTGT GGGCGATGCC TATAGCGCGG TGAGGCTGAA GGAAATTCCT TACACGCTGC GCGAGGCGGC TGCGGCGCTC AAAGGTTCCG CTTTCCTGAA AGAAGCCTTC GGCGAGGATG TCGTGAACCA TTACACCCAT ACCGCCCATT GGGAGCAGAT CGAATATGAC CGCCGCGTGA CCGACTGGGA GCTCTACCGC GGATTTGAAC GCTAC
Details for BrmeB.00111.a.B1.GE36869
HARVESTED ON: 5/1/2013
SEQUENCED ON: 5/8/2013
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 90
Validated AA Sequence
MQGRLIGKRF YGQFFVESGY DETHGCNYLL ADDIDMEPVP GYEAAGWDKG YGDFVMKPDL STLRLAPWLE KTAIVLCDVL DHHHDDLSHS PRAVLKKQVQ RLHERGYRAY FASELEFYIF DETYKSARAK RWHEMETASP YVQGYVIHLT TREEPVLRAM RNHLADAGIP VENSKGEWGP GQQELNVRYC KALEMADRHV IMKNAMKEIA EAHGKCITFM AKYDYARAGS SSHVHNSIWS ADGKEPLFFD PKAPYTMTPL MRSWVAGQIK YATDYTYFLA PYINSYKRFQ AGTFTPTKIM WSQDNRTAGF RLCGEGTKGI RIECRIGGAD INPYLAFAAL IAAGLKGVDE KLELDEPFVG DAYSAVRLKE IPYTLREAAA ALKGSAFLKE AFGEDVVNHY THTAHWEQIE YDRRVTD
Validated NT Sequence
agtcggtcac gcggcggtca tattcgatct gctcccaatg ggcggtatgg gtgtaatggt tcacgacatc ctcgccgaag gcttctttca ggaaagcgga acctttgagc gccgcagccg cctcgcgcag cgtgtaagga atttccttca gcctcaccgc gctataggca tcgcccacaa aaggttcgtc cagttccagc ttttcatcca cgcctttcag gccggcggcg atgagcgctg caaaggccag atagggattg atatccgcgc cgccgatgcg gcattcgatg cgaatgccct tggtaccttc gccgcaaagg cgaaaaccgg ctgtgcggtt gtcctggctc cacatgatct tggtgggggt gaaggtgccc gcctgaaacc gcttgtagga attgatgtaa ggcgccagaa aataggtgta atcggtcgca tatttgatct ggccggccac ccatgagcgc atcagcggtg tcatggtgta gggggctttg ggatcgaaga acagaggttc cttgccatct gcactccaga tggaattgtg cacatggctc gaactgccag cccttgcata atcatacttc gccatgaagg tgatgcactt gccatgcgcc tcggcaatct ccttcatcgc gtttttcatg atgacgtggc gatcggccat ttcaagcgcc ttgcaatagc gcacgttcag ctcctgctgg cccggccccc attcgccttt tgaattttcc accggaatgc ccgcatcggc cagatggttg cgcatggcgc gcaggaccgg ctcctccctg gtggtgaggt ggatcacata accctgcacg taaggcgagg cggtttccat ctcatgccag cgttttgccc gcgccgattt ataggtttca tcgaaaatgt agaattcaag ctcggatgcg aaataagcgc gataaccgcg ctcgtgcagg cgctgcacct gttttttcag gacggcgcgc ggcgaatggg aaagatcgtc atgatggtgg tccagtacat cgcacagcac aatggccgtc ttttccagcc acggcgcaag gcgcagcgtg gagagatccg gcttcatgac gaaatcgcca tagcctttgt cccaacccgc cgcctcatag cccggtacgg gttccatatc gatatcgtcg gccagaagat aattgcagcc gtgggtttcg tcataaccgg attccacgaa aaactgtccg tagaagcgct ttccaatcag gcggccctgc atgtccacga tgcaggccag caccgtgtcg atcctgcctt ccgcgactgc cttcttcaat tcgtcaaaag tcaactgt
Expected Protein Sequence
MAHHHHHHMA GQLTFDELKK AVAEGRIDTV LACIVDMQGR LIGKRFYGQF FVESGYDETH GCNYLLADDI DMEPVPGYEA AGWDKGYGDF VMKPDLSTLR LAPWLEKTAI VLCDVLDHHH DDLSHSPRAV LKKQVQRLHE RGYRAYFASE LEFYIFDETY KSARAKRWHE METASPYVQG YVIHLTTREE PVLRAMRNHL ADAGIPVENS KGEWGPGQQE LNVRYCKALE MADRHVIMKN AMKEIAEAHG KCITFMAKYD YARAGSSSHV HNSIWSADGK EPLFFDPKAP YTMTPLMRSW VAGQIKYATD YTYFLAPYIN SYKRFQAGTF TPTKIMWSQD NRTAGFRLCG EGTKGIRIEC RIGGADINPY LAFAALIAAG LKGVDEKLEL DEPFVGDAYS AVRLKEIPYT LREAAAALKG SAFLKEAFGE DVVNHYTHTA HWEQIEYDRR VTDWELYRGF ERY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccg gacagttgac ttttgacgaa ttgaagaagg cagtcgcgga aggcaggatc gacacggtgc tggcctgcat cgtggacatg cagggccgcc tgattggaaa gcgcttctac ggacagtttt tcgtggaatc cggttatgac gaaacccacg gctgcaatta tcttctggcc gacgatatcg atatggaacc cgtaccgggc tatgaggcgg cgggttggga caaaggctat ggcgatttcg tcatgaagcc ggatctctcc acgctgcgcc ttgcgccgtg gctggaaaag acggccattg tgctgtgcga tgtactggac caccatcatg acgatctttc ccattcgccg cgcgccgtcc tgaaaaaaca ggtgcagcgc ctgcacgagc gcggttatcg cgcttatttc gcatccgagc ttgaattcta cattttcgat gaaacctata aatcggcgcg ggcaaaacgc tggcatgaga tggaaaccgc ctcgccttac gtgcagggtt atgtgatcca cctcaccacc agggaggagc cggtcctgcg cgccatgcgc aaccatctgg ccgatgcggg cattccggtg gaaaattcaa aaggcgaatg ggggccgggc cagcaggagc tgaacgtgcg ctattgcaag gcgcttgaaa tggccgatcg ccacgtcatc atgaaaaacg cgatgaagga gattgccgag gcgcatggca agtgcatcac cttcatggcg aagtatgatt atgcaagggc tggcagttcg agccatgtgc acaattccat ctggagtgca gatggcaagg aacctctgtt cttcgatccc aaagccccct acaccatgac accgctgatg cgctcatggg tggccggcca gatcaaatat gcgaccgatt acacctattt tctggcgcct tacatcaatt cctacaagcg gtttcaggcg ggcaccttca cccccaccaa gatcatgtgg agccaggaca accgcacagc cggttttcgc ctttgcggcg aaggtaccaa gggcattcgc atcgaatgcc gcatcggcgg cgcggatatc aatccctatc tggcctttgc agcgctcatc gccgccggcc tgaaaggcgt ggatgaaaag ctggaactgg acgaaccttt tgtgggcgat gcctatagcg cggtgaggct gaaggaaatt ccttacacgc tgcgcgaggc ggctgcggcg ctcaaaggtt ccgctttcct gaaagaagcc ttcggcgagg atgtcgtgaa ccattacacc cataccgccc attgggagca gatcgaatat gaccgccgcg tgaccgactg ggagctctac cgcggatttg aacgctactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BrmeB.00111.a.B1.PW37375
PURIFICATION DATe: 10/9/2013
CONCENTRATION: 16.5mg/ml
OBSERVED MW: 51kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 90
Protocol Notes
notes unavailable
Validated AA Sequence
MQGRLIGKRF YGQFFVESGY DETHGCNYLL ADDIDMEPVP GYEAAGWDKG YGDFVMKPDL STLRLAPWLE KTAIVLCDVL DHHHDDLSHS PRAVLKKQVQ RLHERGYRAY FASELEFYIF DETYKSARAK RWHEMETASP YVQGYVIHLT TREEPVLRAM RNHLADAGIP VENSKGEWGP GQQELNVRYC KALEMADRHV IMKNAMKEIA EAHGKCITFM AKYDYARAGS SSHVHNSIWS ADGKEPLFFD PKAPYTMTPL MRSWVAGQIK YATDYTYFLA PYINSYKRFQ AGTFTPTKIM WSQDNRTAGF RLCGEGTKGI RIECRIGGAD INPYLAFAAL IAAGLKGVDE KLELDEPFVG DAYSAVRLKE IPYTLREAAA ALKGSAFLKE AFGEDVVNHY THTAHWEQIE YDRRVTD
Validated NT Sequence
agtcggtcac gcggcggtca tattcgatct gctcccaatg ggcggtatgg gtgtaatggt tcacgacatc ctcgccgaag gcttctttca ggaaagcgga acctttgagc gccgcagccg cctcgcgcag cgtgtaagga atttccttca gcctcaccgc gctataggca tcgcccacaa aaggttcgtc cagttccagc ttttcatcca cgcctttcag gccggcggcg atgagcgctg caaaggccag atagggattg atatccgcgc cgccgatgcg gcattcgatg cgaatgccct tggtaccttc gccgcaaagg cgaaaaccgg ctgtgcggtt gtcctggctc cacatgatct tggtgggggt gaaggtgccc gcctgaaacc gcttgtagga attgatgtaa ggcgccagaa aataggtgta atcggtcgca tatttgatct ggccggccac ccatgagcgc atcagcggtg tcatggtgta gggggctttg ggatcgaaga acagaggttc cttgccatct gcactccaga tggaattgtg cacatggctc gaactgccag cccttgcata atcatacttc gccatgaagg tgatgcactt gccatgcgcc tcggcaatct ccttcatcgc gtttttcatg atgacgtggc gatcggccat ttcaagcgcc ttgcaatagc gcacgttcag ctcctgctgg cccggccccc attcgccttt tgaattttcc accggaatgc ccgcatcggc cagatggttg cgcatggcgc gcaggaccgg ctcctccctg gtggtgaggt ggatcacata accctgcacg taaggcgagg cggtttccat ctcatgccag cgttttgccc gcgccgattt ataggtttca tcgaaaatgt agaattcaag ctcggatgcg aaataagcgc gataaccgcg ctcgtgcagg cgctgcacct gttttttcag gacggcgcgc ggcgaatggg aaagatcgtc atgatggtgg tccagtacat cgcacagcac aatggccgtc ttttccagcc acggcgcaag gcgcagcgtg gagagatccg gcttcatgac gaaatcgcca tagcctttgt cccaacccgc cgcctcatag cccggtacgg gttccatatc gatatcgtcg gccagaagat aattgcagcc gtgggtttcg tcataaccgg attccacgaa aaactgtccg tagaagcgct ttccaatcag gcggccctgc atgtccacga tgcaggccag caccgtgtcg atcctgcctt ccgcgactgc cttcttcaat tcgtcaaaag tcaactgt
Expressed Protein Sequence
MAHHHHHHMA GQLTFDELKK AVAEGRIDTV LACIVDMQGR LIGKRFYGQF FVESGYDETH GCNYLLADDI DMEPVPGYEA AGWDKGYGDF VMKPDLSTLR LAPWLEKTAI VLCDVLDHHH DDLSHSPRAV LKKQVQRLHE RGYRAYFASE LEFYIFDETY KSARAKRWHE METASPYVQG YVIHLTTREE PVLRAMRNHL ADAGIPVENS KGEWGPGQQE LNVRYCKALE MADRHVIMKN AMKEIAEAHG KCITFMAKYD YARAGSSSHV HNSIWSADGK EPLFFDPKAP YTMTPLMRSW VAGQIKYATD YTYFLAPYIN SYKRFQAGTF TPTKIMWSQD NRTAGFRLCG EGTKGIRIEC RIGGADINPY LAFAALIAAG LKGVDEKLEL DEPFVGDAYS AVRLKEIPYT LREAAAALKG SAFLKEAFGE DVVNHYTHTA HWEQIEYDRR VTDWELYRGF ERY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCG GACAGTTGAC TTTTGACGAA TTGAAGAAGG CAGTCGCGGA AGGCAGGATC GACACGGTGC TGGCCTGCAT CGTGGACATG CAGGGCCGCC TGATTGGAAA GCGCTTCTAC GGACAGTTTT TCGTGGAATC CGGTTATGAC GAAACCCACG GCTGCAATTA TCTTCTGGCC GACGATATCG ATATGGAACC CGTACCGGGC TATGAGGCGG CGGGTTGGGA CAAAGGCTAT GGCGATTTCG TCATGAAGCC GGATCTCTCC ACGCTGCGCC TTGCGCCGTG GCTGGAAAAG ACGGCCATTG TGCTGTGCGA TGTACTGGAC CACCATCATG ACGATCTTTC CCATTCGCCG CGCGCCGTCC TGAAAAAACA GGTGCAGCGC CTGCACGAGC GCGGTTATCG CGCTTATTTC GCATCCGAGC TTGAATTCTA CATTTTCGAT GAAACCTATA AATCGGCGCG GGCAAAACGC TGGCATGAGA TGGAAACCGC CTCGCCTTAC GTGCAGGGTT ATGTGATCCA CCTCACCACC AGGGAGGAGC CGGTCCTGCG CGCCATGCGC AACCATCTGG CCGATGCGGG CATTCCGGTG GAAAATTCAA AAGGCGAATG GGGGCCGGGC CAGCAGGAGC TGAACGTGCG CTATTGCAAG GCGCTTGAAA TGGCCGATCG CCACGTCATC ATGAAAAACG CGATGAAGGA GATTGCCGAG GCGCATGGCA AGTGCATCAC CTTCATGGCG AAGTATGATT ATGCAAGGGC TGGCAGTTCG AGCCATGTGC ACAATTCCAT CTGGAGTGCA GATGGCAAGG AACCTCTGTT CTTCGATCCC AAAGCCCCCT ACACCATGAC ACCGCTGATG CGCTCATGGG TGGCCGGCCA GATCAAATAT GCGACCGATT ACACCTATTT TCTGGCGCCT TACATCAATT CCTACAAGCG GTTTCAGGCG GGCACCTTCA CCCCCACCAA GATCATGTGG AGCCAGGACA ACCGCACAGC CGGTTTTCGC CTTTGCGGCG AAGGTACCAA GGGCATTCGC ATCGAATGCC GCATCGGCGG CGCGGATATC AATCCCTATC TGGCCTTTGC AGCGCTCATC GCCGCCGGCC TGAAAGGCGT GGATGAAAAG CTGGAACTGG ACGAACCTTT TGTGGGCGAT GCCTATAGCG CGGTGAGGCT GAAGGAAATT CCTTACACGC TGCGCGAGGC GGCTGCGGCG CTCAAAGGTT CCGCTTTCCT GAAAGAAGCC TTCGGCGAGG ATGTCGTGAA CCATTACACC CATACCGCCC ATTGGGAGCA GATCGAATAT GACCGCCGCG TGACCGACTG GGAGCTCTAC CGCGGATTTG AACGCTACTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT