SSGCID: Target Page

BrmeB.00107.d

MEMBRANE FUSION PROTEIN MTRC

CENTER ID:	BrmeB.00107.d
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.00107.d.B1.GE36867	Full length( BrmeB.00107.d )		1	328

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.00107.d.B1.PS01886	Full length( BrmeB.00107.d )		1	328

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_3079
BV-BRC:	fig\|546272.3.peg.3079
RefSeq:	YP_002734551.1
UniProt:	C0RLW4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLRNTVGTAV AIASTAVNSP EAGNVAVLAV KDGAIVKAGD LIAQLDDKAI RANIVKDTAS LAKSQATLNG AEIQLKRTQD LVRKGGATSQ SGDDALAALK VAQAQLQVDQ AQLAADQVTL SYTKIVAPFT GQLGVVQVSP GAYLSAGSSV ATLTRMSPIY AEFTLPETDL ARIRAALKDG TLKTDVTVTS GEGKDVTESG RVDFIDNAVD PASGTVRLRA TLDNRSGNFW PGQSLRISVE LGKIDDLVLV PDVAVEPQEN GSISYVVKPD NSVEIRPVSV ALRADGMAGV RAGLQPGERV VIEGQDALSN GNRVKLVETK PDAKVSSD

NT Sequence

GTGCTGCGCA ATACGGTCGG CACTGCCGTG GCAATAGCGT CAACGGCGGT CAACAGCCCG GAAGCGGGCA ATGTGGCGGT GTTGGCAGTG AAGGATGGTG CCATTGTCAA GGCGGGCGAC CTTATCGCCC AGCTTGACGA CAAGGCAATC CGCGCAAATA TCGTAAAGGA TACGGCTTCC CTCGCGAAGT CGCAGGCCAC GCTGAACGGT GCGGAAATTC AGCTCAAACG TACGCAGGAT CTGGTTCGGA AGGGGGGCGC CACGTCGCAG AGTGGCGATG ATGCCCTGGC CGCATTGAAG GTGGCGCAAG CACAGCTACA GGTCGATCAG GCGCAACTTG CGGCGGATCA GGTGACGCTT TCCTATACAA AAATCGTGGC GCCCTTTACC GGGCAACTGG GCGTCGTTCA GGTTTCTCCG GGTGCTTATC TTTCTGCTGG CTCATCGGTT GCTACCCTCA CCCGGATGTC ACCGATCTAT GCGGAGTTCA CTCTTCCCGA AACGGACCTT GCGCGCATAA GGGCAGCCCT CAAGGACGGC ACGTTGAAAA CGGATGTGAC GGTGACTTCC GGCGAGGGAA AGGATGTCAC CGAAAGCGGC CGCGTCGATT TCATCGACAA TGCAGTCGAT CCGGCCAGTG GTACAGTGCG CTTGCGGGCG ACGCTCGACA ACCGATCCGG CAATTTCTGG CCGGGACAAT CCCTGCGCAT TTCAGTTGAA TTGGGTAAGA TCGATGATCT GGTGCTGGTT CCCGATGTCG CCGTGGAGCC ACAGGAAAAC GGTTCCATCA GTTATGTGGT CAAGCCTGAC AATTCCGTCG AAATCAGACC TGTGAGCGTG GCCTTGCGTG CTGATGGCAT GGCCGGCGTG CGGGCAGGCC TTCAACCGGG CGAAAGAGTG GTTATCGAAG GGCAGGATGC TCTTTCCAAC GGCAACCGGG TAAAACTGGT CGAGACGAAG CCAGATGCCA AGGTTTCATC CGAC

Details for BrmeB.00107.d.B1.GE36867

HARVESTED ON:	5/1/2013
SEQUENCED ON:	5/8/2013
EXPECTED MW:	35kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	51

Validated AA Sequence

MSPIYAEFTL PETDLARIRA ALKDGTLKTD VTVTSGEGKD VTESGRVDFI DNAVDPASGT VRLRATLDNR SGNFWPGQSL RISVELGKID DLVLVPDVAV EPQENGSISY VVKPDNSVEI RPVSVALRAD GMAGVRAGLQ PGERVVIEGQ DALSNGNRVK LVETKPDAKV SSD

Validated NT Sequence

cttagtcgga tgaaaccttg gcatctggct tcgtctcgac cagttttacc cggttgccgt tggaaagagc atcctgccct tcgataacca ctctttcgcc cggttgaagg cctgcccgca cgccggccat gccatcagca cgcaaggcca cgctcacagg tctgatttcg acggaattgt caggcttgac cacataactg atggaaccgt tttcctgtgg ctccacggcg acatcgggaa ccagcaccag atcatcgatc ttacccaatt caactgaaat gcgcagggat tgtcccggcc agaaattgcc ggatcggttg tcgagcgtcg cccgcaagcg cactgtacca ctggccggat cgactgcatt gtcgatgaaa tcgacgcggc cgctttcggt gacatccttt ccctcgccgg aagtcaccgt cacatccgtt ttcaacgtgc cgtccttgag ggctgccctt atgcgcgcaa ggtccgtttc gggaagagtg aactccgcat agatcggtga catccgggtg agggtagcaa ccgatgagcc agcagaaaga taagcacccg gagaaacctg aacgacgccc agttgcccgg taaagggcgc cacgattttt gtataggaaa gcgtcacctg atccgccgca agttgcgcct gatcgacctg tagctgtgct tgcgccacct tcaatgcggc cagggcatca tcgccactct gcgacgtggc gccccccttc cgaaccagat cctgcgtacg tttgagctga atttccgcac cgttcagcgt ggcctgcgac ttcgcgaggg aagccgtatc ctttacgata tttgcgcgga ttgccttgtc gtcaagctgg gcgataaggt cgcccgcctt gacaatggca ccatccttca ctgccaacac cgccacattg cccgcttccg ggctgttgac cgccgttgac gctattgcca cggcagtgcc gaccgtattg cgcagcac

Expected Protein Sequence

MAHHHHHHML RNTVGTAVAI ASTAVNSPEA GNVAVLAVKD GAIVKAGDLI AQLDDKAIRA NIVKDTASLA KSQATLNGAE IQLKRTQDLV RKGGATSQSG DDALAALKVA QAQLQVDQAQ LAADQVTLSY TKIVAPFTGQ LGVVQVSPGA YLSAGSSVAT LTRMSPIYAE FTLPETDLAR IRAALKDGTL KTDVTVTSGE GKDVTESGRV DFIDNAVDPA SGTVRLRATL DNRSGNFWPG QSLRISVELG KIDDLVLVPD VAVEPQENGS ISYVVKPDNS VEIRPVSVAL RADGMAGVRA GLQPGERVVI EGQDALSNGN RVKLVETKPD AKVSSD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgctgc gcaatacggt cggcactgcc gtggcaatag cgtcaacggc ggtcaacagc ccggaagcgg gcaatgtggc ggtgttggca gtgaaggatg gtgccattgt caaggcgggc gaccttatcg cccagcttga cgacaaggca atccgcgcaa atatcgtaaa ggatacggct tccctcgcga agtcgcaggc cacgctgaac ggtgcggaaa ttcagctcaa acgtacgcag gatctggttc ggaagggggg cgccacgtcg cagagtggcg atgatgccct ggccgcattg aaggtggcgc aagcacagct acaggtcgat caggcgcaac ttgcggcgga tcaggtgacg ctttcctata caaaaatcgt ggcgcccttt accgggcaac tgggcgtcgt tcaggtttct ccgggtgctt atctttctgc tggctcatcg gttgctaccc tcacccggat gtcaccgatc tatgcggagt tcactcttcc cgaaacggac cttgcgcgca taagggcagc cctcaaggac ggcacgttga aaacggatgt gacggtgact tccggcgagg gaaaggatgt caccgaaagc ggccgcgtcg atttcatcga caatgcagtc gatccggcca gtggtacagt gcgcttgcgg gcgacgctcg acaaccgatc cggcaatttc tggccgggac aatccctgcg catttcagtt gaattgggta agatcgatga tctggtgctg gttcccgatg tcgccgtgga gccacaggaa aacggttcca tcagttatgt ggtcaagcct gacaattccg tcgaaatcag acctgtgagc gtggccttgc gtgctgatgg catggccggc gtgcgggcag gccttcaacc gggcgaaaga gtggttatcg aagggcagga tgctctttcc aacggcaacc gggtaaaact ggtcgagacg aagccagatg ccaaggtttc atccgactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for BrmeB.00107.d.B1.PS01886

PURIFICATION DATe:	8/22/2013
CONCENTRATION:	40mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	51

Protocol Notes

notes unavailable

Validated AA Sequence

MSPIYAEFTL PETDLARIRA ALKDGTLKTD VTVTSGEGKD VTESGRVDFI DNAVDPASGT VRLRATLDNR SGNFWPGQSL RISVELGKID DLVLVPDVAV EPQENGSISY VVKPDNSVEI RPVSVALRAD GMAGVRAGLQ PGERVVIEGQ DALSNGNRVK LVETKPDAKV SSD

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGCTGC GCAATACGGT CGGCACTGCC GTGGCAATAG CGTCAACGGC GGTCAACAGC CCGGAAGCGG GCAATGTGGC GGTGTTGGCA GTGAAGGATG GTGCCATTGT CAAGGCGGGC GACCTTATCG CCCAGCTTGA CGACAAGGCA ATCCGCGCAA ATATCGTAAA GGATACGGCT TCCCTCGCGA AGTCGCAGGC CACGCTGAAC GGTGCGGAAA TTCAGCTCAA ACGTACGCAG GATCTGGTTC GGAAGGGGGG CGCCACGTCG CAGAGTGGCG ATGATGCCCT GGCCGCATTG AAGGTGGCGC AAGCACAGCT ACAGGTCGAT CAGGCGCAAC TTGCGGCGGA TCAGGTGACG CTTTCCTATA CAAAAATCGT GGCGCCCTTT ACCGGGCAAC TGGGCGTCGT TCAGGTTTCT CCGGGTGCTT ATCTTTCTGC TGGCTCATCG GTTGCTACCC TCACCCGGAT GTCACCGATC TATGCGGAGT TCACTCTTCC CGAAACGGAC CTTGCGCGCA TAAGGGCAGC CCTCAAGGAC GGCACGTTGA AAACGGATGT GACGGTGACT TCCGGCGAGG GAAAGGATGT CACCGAAAGC GGCCGCGTCG ATTTCATCGA CAATGCAGTC GATCCGGCCA GTGGTACAGT GCGCTTGCGG GCGACGCTCG ACAACCGATC CGGCAATTTC TGGCCGGGAC AATCCCTGCG CATTTCAGTT GAATTGGGTA AGATCGATGA TCTGGTGCTG GTTCCCGATG TCGCCGTGGA GCCACAGGAA AACGGTTCCA TCAGTTATGT GGTCAAGCCT GACAATTCCG TCGAAATCAG ACCTGTGAGC GTGGCCTTGC GTGCTGATGG CATGGCCGGC GTGCGGGCAG GCCTTCAACC GGGCGAAAGA GTGGTTATCG AAGGGCAGGA TGCTCTTTCC AACGGCAACC GGGTAAAACT GGTCGAGACG AAGCCAGATG CCAAGGTTTC ATCCGACTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT