BrmeB.00107.b

Multidrug resistance protein A

CENTER ID: BrmeB.00107.b
ORGANISM: Brucella melitensis ATCC 23457
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.00107.b.B1.GE36865 Full length( BrmeB.00107.b ) 1 319
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel14466_1117
BV-BRC: fig|546272.3.peg.1117
RefSeq: YP_002732796.1
UniProt: C0RJ34

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEDDSYVGA AAVAITPQVS GQVIRVAVGP NQPVKEGDLL FEIDPQPFQI ELDQAKANLA QASEKLAGLV LTYKQQLASV AQAKDDVTFA QEQFNRVSAL VHDKFEARAQ FDQAERALQV AKQEQNAAAS AAAATLAQLG GTLDKPLQQH ASYLAAKAEV ELAERNVRLT RVLAPFAGTV TQVENIQPGS FLAVGDPAFT LIGADAWVNS NIKETDLTHI KVGDPATVVL DSYLDQPLQG EVQSITPASG SVFALLPAQN AAGNWVKVVQ RMPVRIKITK PHPDVVMRDG GSATVTINTG YHRTFATLWR DLTGMVGID
NT Sequence
GTGAGCGAGG ATGATTCCTA TGTCGGCGCG GCGGCAGTGG CCATAACACC GCAGGTTTCC GGTCAGGTTA TACGTGTCGC CGTTGGCCCG AACCAGCCCG TCAAGGAAGG CGATCTTCTG TTTGAAATAG ACCCACAGCC GTTCCAGATC GAACTTGACC AGGCAAAGGC CAATCTGGCG CAAGCGAGCG AAAAACTCGC AGGGCTTGTC CTCACCTACA AGCAACAATT GGCAAGTGTG GCTCAGGCAA AGGACGATGT CACCTTCGCA CAGGAGCAAT TCAACCGTGT TTCGGCACTG GTTCACGACA AGTTTGAGGC GCGCGCGCAA TTCGATCAGG CTGAGCGGGC CTTGCAGGTG GCCAAGCAGG AGCAAAATGC CGCCGCGTCG GCAGCCGCCG CAACACTGGC ACAGCTTGGC GGCACACTCG ATAAACCGTT ACAACAACAT GCATCCTATC TGGCGGCCAA AGCCGAAGTG GAGCTTGCCG AACGCAATGT CAGGCTAACC CGCGTGCTCG CCCCATTCGC CGGAACAGTG ACGCAAGTGG AAAACATCCA GCCGGGAAGT TTTCTTGCCG TAGGCGATCC GGCTTTCACC CTGATCGGCG CCGATGCCTG GGTAAACAGC AATATCAAGG AAACGGACCT GACCCATATC AAGGTTGGCG ATCCTGCCAC GGTCGTGCTC GATTCCTATC TGGACCAGCC CCTGCAAGGC GAGGTGCAGA GCATAACGCC CGCAAGCGGT TCTGTTTTTG CCCTGCTGCC CGCCCAGAAT GCCGCCGGCA ACTGGGTCAA GGTGGTGCAG CGCATGCCGG TGCGGATCAA GATTACAAAA CCCCATCCTG ACGTGGTGAT GCGTGATGGC GGCAGCGCAA CCGTTACCAT CAATACAGGC TATCATCGCA CATTTGCTAC CTTGTGGCGC GATCTGACCG GCATGGTGGG GATCGAC
Details for BrmeB.00107.b.B1.GE36865
HARVESTED ON: 5/1/2013
SEQUENCED ON: 5/8/2013
EXPECTED MW: 35kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV SEDDSYVGAA AVAITPQVSG QVIRVAVGPN QPVKEGDLLF EIDPQPFQIE LDQAKANLAQ ASEKLAGLVL TYKQQLASVA QAKDDVTFAQ KQFNRVSALV HDKFEARAQF DQAERALQVA KQEQNAAASA AAATLAQLGG TLDKPLQQHA SYLAAKAEVE LAERNVRLTR VLAPFAGTVT QVENIQPGSF LAVGDPAFTL IGADAWVNSN IKETDLTHIK VGDPATVVLD SYLDQPLQGE VQSITPASGS VFALLPAQNA AGNWVKVVQR MPVRIKITKP HPDVVMRDGG SATVTINTGY HRTFATLWRD LTGMVGID
Validated NT Sequence
tactcactta gtcgatcccc accatgccgg tcagatcgcg ccacaaggta gcaaatgtgc gatgatagcc tgtattgatg gtaacggttg cgctgccgcc atcacgcatc accacgtcag gatggggttt tgtaatcttg atccgcaccg gcatgcgctg caccaccttg acccagttgc cggcggcatt ctgggcgggc agcagggcaa aaacagaacc gcttgcgggc gttatgctct gcacctcgcc ttgcaggggc tggtccagat aggaatcgag cacgaccgtg gcaggatcgc caaccttgat atgggtcagg tccgtttcct tgatattgct gtttacccag gcatcggcgc cgatcagggt gaaagccgga tcgcctacgg caagaaaact tcccggctgg atgttttcca cttgcgtcac tgttccggcg aatggggcga gcacgcgggt tagcctgaca ttgcgttcgg caagctccac ttcggctttg gccgccagat aggatgcatg ttgttgtaac ggtttatcga gtgtgccgcc aagctgtgcc agtgttgcgg cggctgccga cgcggcggca ttttgctcct gcttggccac ctgcaaggcc cgctcagcct gatcgaattg cgcgcgcgcc tcaaacttgt cgtgaaccag tgccgaaaca cggttgaatt gcttctgtgc gaaggtgaca tcgtcctttg cctgagccac acttgccaat tgttgcttgt aggtgaggac aagccctgcg agtttttcgc tcgcttgcgc cagattggcc tttgcctggt caagttcgat ctggaacggc tgtgggtcta tttcaaacag aagatcgcct tccttgacgg gctggttcgg gccaacggcg acacgtataa cctgaccgga aacctgcggt gttatggcca ctgccgccgc gccgacatag gaatcatcct cgctcaccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMS EDDSYVGAAA VAITPQVSGQ VIRVAVGPNQ PVKEGDLLFE IDPQPFQIEL DQAKANLAQA SEKLAGLVLT YKQQLASVAQ AKDDVTFAQE QFNRVSALVH DKFEARAQFD QAERALQVAK QEQNAAASAA AATLAQLGGT LDKPLQQHAS YLAAKAEVEL AERNVRLTRV LAPFAGTVTQ VENIQPGSFL AVGDPAFTLI GADAWVNSNI KETDLTHIKV GDPATVVLDS YLDQPLQGEV QSITPASGSV FALLPAQNAA GNWVKVVQRM PVRIKITKPH PDVVMRDGGS ATVTINTGYH RTFATLWRDL TGMVGID
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagcg aggatgattc ctatgtcggc gcggcggcag tggccataac accgcaggtt tccggtcagg ttatacgtgt cgccgttggc ccgaaccagc ccgtcaagga aggcgatctt ctgtttgaaa tagacccaca gccgttccag atcgaacttg accaggcaaa ggccaatctg gcgcaagcga gcgaaaaact cgcagggctt gtcctcacct acaagcaaca attggcaagt gtggctcagg caaaggacga tgtcaccttc gcacaggagc aattcaaccg tgtttcggca ctggttcacg acaagtttga ggcgcgcgcg caattcgatc aggctgagcg ggccttgcag gtggccaagc aggagcaaaa tgccgccgcg tcggcagccg ccgcaacact ggcacagctt ggcggcacac tcgataaacc gttacaacaa catgcatcct atctggcggc caaagccgaa gtggagcttg ccgaacgcaa tgtcaggcta acccgcgtgc tcgccccatt cgccggaaca gtgacgcaag tggaaaacat ccagccggga agttttcttg ccgtaggcga tccggctttc accctgatcg gcgccgatgc ctgggtaaac agcaatatca aggaaacgga cctgacccat atcaaggttg gcgatcctgc cacggtcgtg ctcgattcct atctggacca gcccctgcaa ggcgaggtgc agagcataac gcccgcaagc ggttctgttt ttgccctgct gcccgcccag aatgccgccg gcaactgggt caaggtggtg cagcgcatgc cggtgcggat caagattaca aaaccccatc ctgacgtggt gatgcgtgat ggcggcagcg caaccgttac catcaataca ggctatcatc gcacatttgc taccttgtgg cgcgatctga ccggcatggt ggggatcgac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat