SSGCID: Target Page

BrmeB.00085.b

UDP-glucose 4-epimerase (EC 5.1.3.2)

CENTER ID:	BrmeB.00085.b
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.00085.b.B1.GE36864	Full length( BrmeB.00085.b )		1	328

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_2800
BV-BRC:	fig\|546272.3.peg.2800
RefSeq:	YP_002734306.1
UniProt:	C0RL69

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSANNVLVVG GAGFIGSHTA KLLAGQGYAP VVYDNLSTGH QSAVRWGDFV EGDILDQARL VETMEKYAPV AVIHFAASAY VGESVEDPAK YYRNNVGGTQ SLLDACRLTR TQNVIFSSSC ATYGVPSRLP IGEGEAQNPI NPYGRTKLIA EHMLADYAVA YGLRYVALRY FNASGADIDG ELGEKHDPET HLIPRAMMAA AGRLDVLEVY GDDYETPDGT CIRDYIHVTD LARAHVLAVE HLKEAGGNLA VNLGTGRGTS SREIVQSIGR LTGRSVPVAM RARRAGDPPA LYADPALAAE KLGFHTVYSD LDTIIRTAAP HFGLEVRG

NT Sequence

ATGTCCGCGA ACAACGTCCT CGTCGTCGGT GGCGCCGGAT TCATCGGCAG CCACACGGCC AAGCTTCTGG CCGGGCAGGG CTATGCACCC GTCGTCTACG ACAATCTTTC GACGGGCCAT CAATCCGCCG TCCGGTGGGG CGACTTTGTC GAGGGCGACA TTCTTGACCA GGCGCGTCTG GTCGAGACAA TGGAAAAATA TGCGCCGGTC GCGGTAATCC ATTTCGCCGC CTCGGCCTAT GTCGGGGAAT CGGTGGAGGA CCCCGCCAAA TATTATCGCA ACAATGTAGG CGGAACGCAG TCGCTTCTCG ACGCCTGCCG TCTGACGCGC ACGCAAAACG TTATCTTCTC CTCCAGTTGC GCCACCTATG GCGTGCCCAG CCGGCTTCCC ATCGGGGAGG GCGAGGCCCA GAATCCGATC AACCCTTATG GGCGCACAAA GTTGATCGCC GAGCACATGC TGGCCGATTA TGCGGTGGCC TACGGGCTGC GTTATGTGGC GCTACGCTAT TTCAATGCAT CCGGTGCCGA TATCGACGGT GAACTGGGCG AAAAGCACGA CCCTGAAACA CATCTCATCC CGCGCGCCAT GATGGCCGCC GCTGGCAGGC TCGACGTTCT GGAAGTCTAT GGCGACGATT ACGAGACGCC GGACGGCACC TGCATCCGCG ACTATATCCA TGTCACCGAT CTCGCGCGGG CGCATGTGCT GGCGGTCGAG CATCTGAAGG AAGCCGGGGG CAATCTGGCC GTTAATCTGG GTACCGGGCG TGGCACGTCA AGTCGTGAGA TCGTGCAGTC CATTGGCCGC CTGACGGGCC GTAGCGTGCC GGTCGCCATG CGCGCGCGCC GTGCCGGCGA TCCGCCAGCG CTCTATGCCG ACCCGGCACT TGCTGCCGAA AAGCTCGGCT TCCATACGGT CTATTCCGAT CTGGACACTA TCATCCGCAC CGCTGCCCCA CACTTCGGAC TGGAGGTTAG GGGA

Details for BrmeB.00085.b.B1.GE36864

HARVESTED ON:	5/1/2013
SEQUENCED ON:	5/8/2013
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

tcttactcac ttatccccta acctccagtc cgaagtgtgg ggcagcggtg cggatgatag tgtccagatc ggaatagacc gtatggaagc cgagcttttc ggcagcaagt gccgggtcgg catagagcgc tggcggatcg ccggcacggc gcgcgcgcat ggcgaccggc acgctacggc ccgtcaggcg gccaatggac tgcacgatct cacgacttga cgtgccacgc ccggtaccca gattaacggc cagattgccc ccggcttcct tcagatgctc gaccgccagc acatgcgccc gcgcgagatc ggtgacatgg atatagtcgc ggatgcaggt gccgtccggc gtctcgtaat cgtcgccata gacttccaga acgtcgagcc tgccagcggc ggccatcatg gcgcgcggga tgagatgtgt ttcagggtcg tgcttttcgc ccagttcacc gtcgatatcg gcaccggatg cattgaaata gcgtagcgcc acataacgca gcccgtaggc caccgcataa tcggccagca tgtgctcggc gatcaacttt gtgcgcccat aagggttgat cggattctgg gcctcgccct ccccgatggg aagccggctg ggcacgccat aggtggcgca actggaggag aagataacgt tttgcgtgcg cgtcagacgg caggcgtcga gaagcgactg cgttccgcct acattgttgc gataatattt ggcggggtcc tccaccgatt ccccgacata ggccgaggcg gcgaaatgga ttaccgcgac cggcgcatat ttttccattg tctcgaccag acgcgcctgg tcaagaatgt cgccctcgac aaagtcgccc caccggacgg cggattgatg gcccgtcgaa agattgtcgt agacgacggg tgcatagccc tgcccggcca gaagcttggc cgtgtggctg ccgatgaatc cggcgccacc gacgacgagg acgttgttcg cggacatatg gtggtggtgg t

Expected Protein Sequence

MAHHHHHHMS ANNVLVVGGA GFIGSHTAKL LAGQGYAPVV YDNLSTGHQS AVRWGDFVEG DILDQARLVE TMEKYAPVAV IHFAASAYVG ESVEDPAKYY RNNVGGTQSL LDACRLTRTQ NVIFSSSCAT YGVPSRLPIG EGEAQNPINP YGRTKLIAEH MLADYAVAYG LRYVALRYFN ASGADIDGEL GEKHDPETHL IPRAMMAAAG RLDVLEVYGD DYETPDGTCI RDYIHVTDLA RAHVLAVEHL KEAGGNLAVN LGTGRGTSSR EIVQSIGRLT GRSVPVAMRA RRAGDPPALY ADPALAAEKL GFHTVYSDLD TIIRTAAPHF GLEVRG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg cgaacaacgt cctcgtcgtc ggtggcgccg gattcatcgg cagccacacg gccaagcttc tggccgggca gggctatgca cccgtcgtct acgacaatct ttcgacgggc catcaatccg ccgtccggtg gggcgacttt gtcgagggcg acattcttga ccaggcgcgt ctggtcgaga caatggaaaa atatgcgccg gtcgcggtaa tccatttcgc cgcctcggcc tatgtcgggg aatcggtgga ggaccccgcc aaatattatc gcaacaatgt aggcggaacg cagtcgcttc tcgacgcctg ccgtctgacg cgcacgcaaa acgttatctt ctcctccagt tgcgccacct atggcgtgcc cagccggctt cccatcgggg agggcgaggc ccagaatccg atcaaccctt atgggcgcac aaagttgatc gccgagcaca tgctggccga ttatgcggtg gcctacgggc tgcgttatgt ggcgctacgc tatttcaatg catccggtgc cgatatcgac ggtgaactgg gcgaaaagca cgaccctgaa acacatctca tcccgcgcgc catgatggcc gccgctggca ggctcgacgt tctggaagtc tatggcgacg attacgagac gccggacggc acctgcatcc gcgactatat ccatgtcacc gatctcgcgc gggcgcatgt gctggcggtc gagcatctga aggaagccgg gggcaatctg gccgttaatc tgggtaccgg gcgtggcacg tcaagtcgtg agatcgtgca gtccattggc cgcctgacgg gccgtagcgt gccggtcgcc atgcgcgcgc gccgtgccgg cgatccgcca gcgctctatg ccgacccggc acttgctgcc gaaaagctcg gcttccatac ggtctattcc gatctggaca ctatcatccg caccgctgcc ccacacttcg gactggaggt taggggatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at