SSGCID: Target Page

BrmeB.00057.f

Glycerol-3-phosphate ABC transporter, ATP-binding protein UgpC (TC 3.A.1.1.3)

CENTER ID:	BrmeB.00057.f
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.00057.f.B1.GE36862	Full length( BrmeB.00057.f )		1	390

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_3469
BV-BRC:	fig\|546272.3.peg.3469
RefSeq:	YP_002734896.1
UniProt:	C0RMV9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTLVLRDIG KSYGGNRVLD GISIEAAEGE FIALVGPSGC GKSTLLRILA GLDHADEGSV ELAGCNITGA HPADRNIAMV FQSYALYPHL TAAQNIAVPL AMRDLTVMER LPVIGAFLPG QRDKRAAIQK QVRATAASLK IDHLLDRKPG QMSGGQRQRV ALARALVRQP GAFLMDEPLS NLDANLRVHT RAEIVELHRR AGVPTIYVTH DQAEALSMAD RVAVMMGGKL LQIDTPGAIY NEPRHIEVAQ FIGTPRINIL EALVSDNGVV HFAGRAILQG VAAHSGSNIR IGIRPEHFQL RPHDKTGIAS TVSRIEFLGS EVLAYLKVPG AGQDLTVKLS PEDARDIVVG LPAGLHFDAS KAYLFGEDGL RQPAGNIVKL ASPAKATAHV

NT Sequence

GTGAGCACAC TCGTCCTTCG CGACATTGGC AAATCCTACG GGGGAAATCG CGTTCTCGAT GGCATTTCGA TCGAAGCCGC AGAAGGCGAA TTCATCGCCC TCGTCGGCCC GTCCGGCTGC GGCAAGAGCA CATTGCTGCG TATTCTTGCC GGGCTCGATC ATGCGGACGA AGGAAGCGTG GAGCTTGCCG GGTGCAACAT AACCGGCGCC CACCCCGCCG ACCGGAACAT TGCTATGGTG TTCCAGTCCT ATGCACTCTA TCCCCATCTG ACAGCGGCGC AGAACATCGC CGTGCCGCTG GCGATGCGCG ATCTGACCGT AATGGAACGC CTGCCGGTGA TCGGCGCCTT CCTGCCCGGC CAGCGCGACA AGCGCGCCGC CATCCAGAAG CAGGTCAGGG CAACCGCGGC CAGCCTCAAG ATCGACCACC TGCTCGACCG CAAGCCGGGC CAGATGTCCG GTGGCCAGCG CCAGCGTGTG GCATTGGCCC GCGCACTTGT CCGCCAGCCC GGCGCATTTC TGATGGACGA ACCGCTGTCC AATCTCGATG CCAATCTGCG CGTTCACACG CGCGCAGAAA TCGTCGAACT GCATCGCCGC GCCGGTGTGC CCACCATCTA TGTGACCCAC GATCAGGCTG AAGCACTCAG CATGGCCGAC CGCGTTGCGG TGATGATGGG CGGCAAACTG CTCCAGATCG ACACGCCTGG CGCGATCTAT AACGAGCCGC GCCATATCGA GGTCGCGCAA TTCATCGGCA CGCCGCGCAT CAATATTCTG GAAGCATTGG TCAGCGATAA CGGCGTGGTG CATTTTGCAG GCCGCGCCAT CCTTCAGGGC GTCGCCGCCC ATTCCGGCAG CAATATTCGT ATTGGCATTC GTCCTGAACA TTTTCAGCTC AGGCCGCACG ACAAGACAGG CATTGCAAGC ACCGTTTCAC GGATCGAATT CCTCGGCTCG GAAGTCCTTG CCTATCTCAA GGTCCCCGGC GCCGGGCAGG ATCTCACGGT GAAGCTTTCA CCGGAAGACG CCCGCGACAT CGTCGTCGGC CTGCCTGCGG GTCTGCATTT TGATGCCAGC AAGGCTTATC TCTTCGGGGA AGATGGCCTG CGCCAGCCGG CCGGAAATAT CGTGAAGCTT GCATCCCCGG CAAAGGCTAC CGCTCATGTC

Details for BrmeB.00057.f.B1.GE36862

HARVESTED ON:	5/1/2013
SEQUENCED ON:	5/8/2013
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	75

Validated AA Sequence

MVFQSYALYP HLTAAQNIAV PLAMRDLTVM ERLPVIGAFL PGQRDKRAAI QKQVRATAAS LKIDHLLDRK PGQMSGGQRQ RVALARALVR QPGAFLMDEP LSNLDANLRV HTRAEIVELH RRAGVPTIYV THDQAEALSM ADRVAVMMGG KLLQIDTPGA IYNEPRHIEV AQFIGTPRIN ILEALVSDNG VVHFAGRAIL QGVAAHSGSN IRIGIRPEHF QLRPHDKTGI ASTVSRIEFL GSEVLAYLKV PGAGQDLTVK LSPEDARDIV VGLPAGLHFD ASKAYLFGED GLRQPAGNIV KL

Validated NT Sequence

gcaagcttca cgatatttcc ggccggctgg cgcaggccat cttccccgaa gagataagcc ttgctggcat caaaatgcag acccgcaggc aggccgacga cgatgtcgcg ggcgtcttcc ggtgaaagct tcaccgtgag atcctgcccg gcgccgggga ccttgagata ggcaaggact tccgagccga ggaattcgat ccgtgaaacg gtgcttgcaa tgcctgtctt gtcgtgcggc ctgagctgaa aatgttcagg acgaatgcca atacgaatat tgctgccgga atgggcggcg acgccctgaa ggatggcgcg gcctgcaaaa tgcaccacgc cgttatcgct gaccaatgct tccagaatat tgatgcgcgg cgtgccgatg aattgcgcga cctcgatatg gcgcggctcg ttatagatcg cgccaggcgt gtcgatctgg agcagtttgc cgcccatcat caccgcaacg cggtcggcca tgctgagtgc ttcagcctga tcgtgggtca catagatggt gggcacaccg gcgcggcgat gcagttcgac gatttctgcg cgcgtgtgaa cgcgcagatt ggcatcgaga ttggacagcg gttcgtccat cagaaatgcg ccgggctggc ggacaagtgc gcgggccaat gccacacgct ggcgctggcc accggacatc tggcccggct tgcggtcgag caggtggtcg atcttgaggc tggccgcggt tgccctgacc tgcttctgga tggcggcgcg cttgtcgcgc tggccgggca ggaaggcgcc gatcaccggc aggcgttcca ttacggtcag atcgcgcatc gccagcggca cggcgatgtt ctgcgccgct gtcagatggg gatagagtgc ataggactgg aacaccatag caatgttccg gtcggcgggg tgggcgccgg ttatgttgca cccggcaagc tccacgcttc cttcgtccgc atgatcgagc ccggcaagaa tacgcagcaa tgtgctcttg ccgcagccgg acgggccgac gagggcgatg aattcgcctt ctgcggcttc gatcgaaatg ccatcgagaa cgcgatttcc cccgtaggat ttgccaatgt cgcgaaggac gagtgtg

Expected Protein Sequence

MAHHHHHHMS TLVLRDIGKS YGGNRVLDGI SIEAAEGEFI ALVGPSGCGK STLLRILAGL DHADEGSVEL AGCNITGAHP ADRNIAMVFQ SYALYPHLTA AQNIAVPLAM RDLTVMERLP VIGAFLPGQR DKRAAIQKQV RATAASLKID HLLDRKPGQM SGGQRQRVAL ARALVRQPGA FLMDEPLSNL DANLRVHTRA EIVELHRRAG VPTIYVTHDQ AEALSMADRV AVMMGGKLLQ IDTPGAIYNE PRHIEVAQFI GTPRINILEA LVSDNGVVHF AGRAILQGVA AHSGSNIRIG IRPEHFQLRP HDKTGIASTV SRIEFLGSEV LAYLKVPGAG QDLTVKLSPE DARDIVVGLP AGLHFDASKA YLFGEDGLRQ PAGNIVKLAS PAKATAHV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagca cactcgtcct tcgcgacatt ggcaaatcct acgggggaaa tcgcgttctc gatggcattt cgatcgaagc cgcagaaggc gaattcatcg ccctcgtcgg cccgtccggc tgcggcaaga gcacattgct gcgtattctt gccgggctcg atcatgcgga cgaaggaagc gtggagcttg ccgggtgcaa cataaccggc gcccaccccg ccgaccggaa cattgctatg gtgttccagt cctatgcact ctatccccat ctgacagcgg cgcagaacat cgccgtgccg ctggcgatgc gcgatctgac cgtaatggaa cgcctgccgg tgatcggcgc cttcctgccc ggccagcgcg acaagcgcgc cgccatccag aagcaggtca gggcaaccgc ggccagcctc aagatcgacc acctgctcga ccgcaagccg ggccagatgt ccggtggcca gcgccagcgt gtggcattgg cccgcgcact tgtccgccag cccggcgcat ttctgatgga cgaaccgctg tccaatctcg atgccaatct gcgcgttcac acgcgcgcag aaatcgtcga actgcatcgc cgcgccggtg tgcccaccat ctatgtgacc cacgatcagg ctgaagcact cagcatggcc gaccgcgttg cggtgatgat gggcggcaaa ctgctccaga tcgacacgcc tggcgcgatc tataacgagc cgcgccatat cgaggtcgcg caattcatcg gcacgccgcg catcaatatt ctggaagcat tggtcagcga taacggcgtg gtgcattttg caggccgcgc catccttcag ggcgtcgccg cccattccgg cagcaatatt cgtattggca ttcgtcctga acattttcag ctcaggccgc acgacaagac aggcattgca agcaccgttt cacggatcga attcctcggc tcggaagtcc ttgcctatct caaggtcccc ggcgccgggc aggatctcac ggtgaagctt tcaccggaag acgcccgcga catcgtcgtc ggcctgcctg cgggtctgca ttttgatgcc agcaaggctt atctcttcgg ggaagatggc ctgcgccagc cggccggaaa tatcgtgaag cttgcatccc cggcaaaggc taccgctcat gtctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat