SSGCID: Target Page

BrmeB.00057.e

Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1)

CENTER ID:	BrmeB.00057.e
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.00057.e.B1.GE36861	Full length( BrmeB.00057.e )		1	347

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_3358
BV-BRC:	fig\|546272.3.peg.3358
RefSeq:	YP_002734804.1
UniProt:	C0RML7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGISIEGVTK KYGSFTAVDG LDMQIAPGEF LALLGPSGSG KSTILMLLAG FERPDNGRIL FGNNDYTRVP PHRRNIGMVF QHYTLFPNMS VLDNVAFPLK TRGMERSQRH RIAREALARV RLADFADRSP KQLSGGQQQR VALARSIVYS PRVLLMDEPL SALDKNLREE MQIEIKRLHT ELGMTIVFVT HDQGEALTMA DRVAILQGGH IQQIAGAREL YNRPANLFSA RFIGEMNLIP VDWDGQTART TDGTTVPVPA HNVMNASTGK ALIAIRPERL APAGQGLEIT VRDVIYAGPD TAVLGTMADG TELRARLASA ALPDIRSGQK LYLGFSPEAG LLYRPAE

NT Sequence

TTGGGTATCA GCATTGAAGG CGTCACCAAG AAATATGGCA GCTTCACCGC AGTCGACGGG CTGGATATGC AGATTGCGCC TGGCGAATTT CTGGCGTTGC TGGGGCCTTC CGGATCGGGA AAGTCCACCA TCCTGATGCT GCTGGCGGGG TTCGAGCGCC CGGATAATGG CCGCATTCTC TTCGGCAATA ATGATTATAC CCGTGTTCCG CCGCACAGGC GCAATATTGG CATGGTATTC CAGCATTACA CCCTGTTTCC CAATATGAGC GTGCTCGATA ATGTGGCGTT CCCGCTCAAG ACGCGGGGCA TGGAGCGCAG CCAGCGCCAC AGGATCGCGC GTGAAGCGCT GGCGCGCGTG CGGCTTGCCG ATTTTGCGGA TCGCAGTCCC AAGCAATTGT CCGGCGGGCA GCAGCAACGC GTCGCGCTGG CACGCTCCAT TGTCTATTCG CCGCGCGTGC TCCTGATGGA TGAGCCGCTT TCGGCCCTGG ACAAGAACCT GCGCGAAGAG ATGCAGATCG AGATCAAGCG CTTGCATACC GAGCTTGGCA TGACGATCGT ATTCGTGACC CATGACCAGG GCGAGGCCCT CACCATGGCG GACAGGGTGG CGATTCTGCA AGGTGGCCAT ATTCAGCAGA TCGCAGGCGC CCGCGAGCTT TACAATAGAC CGGCAAACCT GTTCTCCGCC AGGTTTATTG GCGAGATGAA TCTGATTCCG GTCGACTGGG ATGGCCAGAC CGCGCGCACG ACCGATGGGA CCACGGTTCC GGTTCCGGCG CACAACGTGA TGAATGCGTC TACCGGGAAG GCACTAATAG CCATTCGGCC CGAACGGCTG GCGCCAGCCG GGCAGGGGCT GGAGATTACT GTCCGGGACG TGATCTACGC CGGGCCCGAT ACGGCTGTTC TGGGAACCAT GGCGGACGGA ACCGAATTGC GTGCCCGTCT GGCAAGCGCA GCGCTTCCGG ATATTCGCAG CGGACAGAAA TTGTATCTGG GCTTCTCGCC GGAAGCCGGC CTGCTCTATC GTCCGGCGGA G

Details for BrmeB.00057.e.B1.GE36861

HARVESTED ON:	5/1/2013
SEQUENCED ON:	5/8/2013
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	93
PERCENT COVERAGE:	102

Validated AA Sequence

MAHHHHHHML GISIEGVTKK YGSFTAVDGL DMQIAPGEFL ALLGPSGSGK STILMLLAGF ERPDNGRILF GNNDYTRVPP HRRNIGMVFQ HYTLFPNMSV LDNVAFPLKT RGMERSQRHR IAREALARVR LADFADRSPK QLSGGQQQRV ALARSIVYSP RVLLMDEPLS ALDKNLREEM QIEIKRLHTE LGMTIVFVTH DQGEALTMAD RVAILQGGHI QQIAGARELY NRPANLFSAR FIGEMNLIPV DWXGQTARTT DGTTVPVPAH NVMNASTGKA LIAIRPERLA PAGQGLEITV RXVIYAGPDT AVLGTMAXGT ELRAXLASXX LPXXXXGXKL XXGXXPEAXA SSGXXXXXXX XXXX

Validated NT Sequence

tttnttnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatgttggg tatcagcatt gaaggcgtca ccaagaaata tggcagcttc accgcagtcg acgggctgga tatgcagatt gcgcctggcg aatttctggc gttgctgggg ccttccggat cgggaaagtc caccatcctg atgctgctgg cggggttcga gcgcccggat aatggccgca ttctcttcgg caataatgat tatacccgtg ttccgccgca caggcgcaat attggcatgg tattccagca ttacaccctg tttcccaata tgagcgtgct cgataatgtg gcgttcccgc tcaagacgcg gggcatggag cgcagccagc gccacaggat cgcgcgtgaa gcgctggcgc gcgtgcggct tgccgatttt gcggatcgca gtcccaagca attgtccggc gggcagcagc aacgcgtcgc gctggcacgc tccattgtct attcgccgcg cgtgctcctg atggatgagc cgctttcggc cctggacaag aacctgcgcg aagagatgca gatcgagatc aagcgcttgc ataccgagct tggcatgacg atcgtattcg tgacccatga ccagggcgag gccctcacca tggcggacag ggtggcgatt ctgcaaggtg gccatattca gcagatcgca ggcgcccgcg agctttacaa tagaccggca aacctgttct ccgccaggtt tattggcgag atgaatctga ttccggtcga ctggnatggc cagaccgcgc gcacgaccga tgggaccacg gttccggttc cggcgcacaa cgtgatgaat gcgtctaccg ggaaggcact aatagccatt cggcccgaac ggctggcgcc agccgggcag gggctggaga ttactgtccg ggnngtgatc tacgccgggc ccgatacggc tgttctggga accatggcgg anggaaccga attgcgtgcc cntctggcaa gcncancgct tccgnannnt cncancggan ngaaattgnn nntgggctnn nnnccngaag ccnnngcntc gtccggcnna nnannnngtn nnnnnnnnnn nnnnnnnnnc c

Expected Protein Sequence

MAHHHHHHMG ISIEGVTKKY GSFTAVDGLD MQIAPGEFLA LLGPSGSGKS TILMLLAGFE RPDNGRILFG NNDYTRVPPH RRNIGMVFQH YTLFPNMSVL DNVAFPLKTR GMERSQRHRI AREALARVRL ADFADRSPKQ LSGGQQQRVA LARSIVYSPR VLLMDEPLSA LDKNLREEMQ IEIKRLHTEL GMTIVFVTHD QGEALTMADR VAILQGGHIQ QIAGARELYN RPANLFSARF IGEMNLIPVD WDGQTARTTD GTTVPVPAHN VMNASTGKAL IAIRPERLAP AGQGLEITVR DVIYAGPDTA VLGTMADGTE LRARLASAAL PDIRSGQKLY LGFSPEAGLL YRPAE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgggta tcagcattga aggcgtcacc aagaaatatg gcagcttcac cgcagtcgac gggctggata tgcagattgc gcctggcgaa tttctggcgt tgctggggcc ttccggatcg ggaaagtcca ccatcctgat gctgctggcg gggttcgagc gcccggataa tggccgcatt ctcttcggca ataatgatta tacccgtgtt ccgccgcaca ggcgcaatat tggcatggta ttccagcatt acaccctgtt tcccaatatg agcgtgctcg ataatgtggc gttcccgctc aagacgcggg gcatggagcg cagccagcgc cacaggatcg cgcgtgaagc gctggcgcgc gtgcggcttg ccgattttgc ggatcgcagt cccaagcaat tgtccggcgg gcagcagcaa cgcgtcgcgc tggcacgctc cattgtctat tcgccgcgcg tgctcctgat ggatgagccg ctttcggccc tggacaagaa cctgcgcgaa gagatgcaga tcgagatcaa gcgcttgcat accgagcttg gcatgacgat cgtattcgtg acccatgacc agggcgaggc cctcaccatg gcggacaggg tggcgattct gcaaggtggc catattcagc agatcgcagg cgcccgcgag ctttacaata gaccggcaaa cctgttctcc gccaggttta ttggcgagat gaatctgatt ccggtcgact gggatggcca gaccgcgcgc acgaccgatg ggaccacggt tccggttccg gcgcacaacg tgatgaatgc gtctaccggg aaggcactaa tagccattcg gcccgaacgg ctggcgccag ccgggcaggg gctggagatt actgtccggg acgtgatcta cgccgggccc gatacggctg ttctgggaac catggcggac ggaaccgaat tgcgtgcccg tctggcaagc gcagcgcttc cggatattcg cagcggacag aaattgtatc tgggcttctc gccggaagcc ggcctgctct atcgtccggc ggagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat