BrmeB.00057.d

ABC transporter, ATP-binding protein

CENTER ID: BrmeB.00057.d
ORGANISM: Brucella melitensis ATCC 23457
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.00057.d.B1.GE36860 Full length( BrmeB.00057.d ) 1 303
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel14466_3071

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNCIAGLLAT TGGGVYIDEK RIDTLKPEDR GFGMVFQNYA LFPHMSVREN VGFGLKMRGT PKSEIRKRVD EAIGMVRLNG QEEKLPGQLS GGQQQRVAIA RAIVIEPPLV LMDEPLSNLD AKLRLEMRAE IRRIHNQLGS TTIYVTHDQD EALSLADRIV VLRDGEIRQV GVPSDLYERP DHLDVAEFMG YRNRLPGKVT AIDGERATIS IGGSLIAGIA RVPLAIGQDV VLAARPDDVA TGPASPAALQ AKVETIEYRG RDFVGTANMS DGHNLVFHAS AHAELGSAVS LTVDPNRALI FPA
NT Sequence
TTGAACTGTA TTGCCGGTCT GCTGGCTACC ACCGGTGGCG GCGTCTATAT CGATGAAAAG CGCATCGATA CATTGAAGCC GGAAGACCGT GGCTTTGGGA TGGTGTTTCA GAATTATGCG CTGTTTCCAC ATATGAGCGT CCGCGAGAAT GTGGGCTTCG GCCTGAAAAT GCGTGGCACA CCGAAGTCAG AAATCCGAAA ACGTGTAGAT GAAGCCATAG GAATGGTTCG TCTCAATGGT CAGGAAGAAA AACTGCCCGG CCAGCTTTCA GGCGGTCAGC AACAACGGGT TGCAATTGCC CGCGCCATCG TCATCGAACC GCCATTGGTT CTTATGGATG AGCCGCTTTC CAATCTCGAT GCCAAACTGC GCCTGGAAAT GCGGGCCGAA ATTCGCCGCA TTCACAATCA GCTTGGCTCG ACCACGATTT ATGTCACGCA CGATCAGGAT GAGGCGCTCT CGCTTGCAGA TCGTATAGTG GTGCTGCGCG ATGGCGAGAT CCGCCAGGTT GGCGTGCCCA GCGATCTTTA CGAGCGTCCC GACCATTTGG ACGTCGCCGA ATTTATGGGA TATCGCAATC GCCTCCCCGG TAAGGTGACG GCAATTGATG GAGAGCGGGC AACTATATCC ATCGGCGGTT CGCTGATTGC AGGTATTGCC CGCGTACCTC TTGCCATAGG TCAGGATGTC GTGCTTGCAG CGCGCCCCGA TGACGTCGCT ACGGGACCTG CAAGCCCAGC GGCCCTTCAA GCAAAAGTCG AGACGATCGA ATATCGCGGA CGTGATTTTG TGGGCACCGC GAACATGTCA GACGGGCATA ATCTTGTGTT TCACGCCTCC GCCCACGCTG AACTGGGAAG TGCAGTTTCT CTGACGGTCG ACCCAAACCG TGCCCTGATT TTCCCGGCG
Details for BrmeB.00057.d.B1.GE36860
HARVESTED ON: 5/1/2013
SEQUENCED ON: 5/8/2013
EXPECTED MW: 34kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML NCIAGLLATT GGGVYIDEKR IDTLKPEDRG FGMVFQNYAL FPHMSVRENV GFGLKMRGTP KSEIRKRVDE AIGMVRLNGQ EEKLPGQLSG GQQQRVAIAR AIVIEPPLVL MDEPLSNLDA KLRLEMRAEI RRIHNQLGST TIYVTHDQDE ALSLADRIVV LRDGEIRQVG VPSDLYERPD HLDVAEFMGY RNRLPGKVTA IDGERATISI GGSLIAGIAR VPLAIGQDVV LAARPDDVAT GPASPAALQA KVETIEYRGR DFVGTANMSX GHNLVFHASA HAELGSAVSL XXDPNRALIF PX
Validated NT Sequence
attttgntta nctttaagaa ggaganatac catggctcac caccaccacc accatatgtt gaactgtatt gccggtctgc tggctaccac cggtggcggc gtctatatcg atgaaaagcg catcgataca ttgaagccgg aagaccgtgg ctttgggatg gtgtttcaga attatgcgct gtttccacat atgagcgtcc gcgagaatgt gggcttcggc ctgaaaatgc gtggcacacc gaagtcagaa atccgaaaac gtgtagatga agccatagga atggttcgtc tcaatggtca ggaagaaaaa ctgcccggcc agctttcagg cggtcagcaa caacgggttg caattgcccg cgccatcgtc atcgaaccgc cattggttct tatggatgag ccgctttcca atctcgatgc caaactgcgc ctggaaatgc gggccgaaat tcgccgcatt cacaatcagc ttggctcgac cacgatttat gtcacgcacg atcaggatga ggcgctctcg cttgcagatc gtatagtggt gctgcgcgat ggcgagatcc gccaggttgg cgtgcccagc gatctttacg agcgtcccga ccatttggac gtcgccgaat ttatgggata tcgcaatcgc ctccccggta aggtgacggc aattgatgga gagcgggcaa ctatatccat cggcggttcg ctgattgcag gtattgcccg cgtacctctt gccataggtc aggatgtcgt gcttgcagcg cgccccgatg acgtcgctac gggacctgca agcccagcgg cccttcaagc aaaagtcgag acgatcgaat atcgcggacg tgattttgtg ggcaccgcga acatgtcana cgggcataat cttgtgtttc acgcctccgc ccacgctgaa ctgggaagtg cagtttctct gangntcgac ccaaaccgtg ccctgatttt cccggngtaa gtgagtaana naggannnnn nnnnnnnnnn cc
Expected Protein Sequence
MAHHHHHHMN CIAGLLATTG GGVYIDEKRI DTLKPEDRGF GMVFQNYALF PHMSVRENVG FGLKMRGTPK SEIRKRVDEA IGMVRLNGQE EKLPGQLSGG QQQRVAIARA IVIEPPLVLM DEPLSNLDAK LRLEMRAEIR RIHNQLGSTT IYVTHDQDEA LSLADRIVVL RDGEIRQVGV PSDLYERPDH LDVAEFMGYR NRLPGKVTAI DGERATISIG GSLIAGIARV PLAIGQDVVL AARPDDVATG PASPAALQAK VETIEYRGRD FVGTANMSDG HNLVFHASAH AELGSAVSLT VDPNRALIFP A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgaact gtattgccgg tctgctggct accaccggtg gcggcgtcta tatcgatgaa aagcgcatcg atacattgaa gccggaagac cgtggctttg ggatggtgtt tcagaattat gcgctgtttc cacatatgag cgtccgcgag aatgtgggct tcggcctgaa aatgcgtggc acaccgaagt cagaaatccg aaaacgtgta gatgaagcca taggaatggt tcgtctcaat ggtcaggaag aaaaactgcc cggccagctt tcaggcggtc agcaacaacg ggttgcaatt gcccgcgcca tcgtcatcga accgccattg gttcttatgg atgagccgct ttccaatctc gatgccaaac tgcgcctgga aatgcgggcc gaaattcgcc gcattcacaa tcagcttggc tcgaccacga tttatgtcac gcacgatcag gatgaggcgc tctcgcttgc agatcgtata gtggtgctgc gcgatggcga gatccgccag gttggcgtgc ccagcgatct ttacgagcgt cccgaccatt tggacgtcgc cgaatttatg ggatatcgca atcgcctccc cggtaaggtg acggcaattg atggagagcg ggcaactata tccatcggcg gttcgctgat tgcaggtatt gcccgcgtac ctcttgccat aggtcaggat gtcgtgcttg cagcgcgccc cgatgacgtc gctacgggac ctgcaagccc agcggccctt caagcaaaag tcgagacgat cgaatatcgc ggacgtgatt ttgtgggcac cgcgaacatg tcagacgggc ataatcttgt gtttcacgcc tccgcccacg ctgaactggg aagtgcagtt tctctgacgg tcgacccaaa ccgtgccctg attttcccgg cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat