SSGCID: Target Page

BrmeB.00057.b

ABC transporter ATP-binding protein

CENTER ID:	BrmeB.00057.b
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.00057.b.B1.GE36858	Full length( BrmeB.00057.b )		1	264

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_2128
BV-BRC:	fig\|546272.3.peg.2128
RefSeq:	YP_002733720.1
UniProt:	C0RFU4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIELSNLDVV FGRGTPLEKQ VLHKINLTMN KGSFVTVIGS NGAGKSTMLG VLAGDVIPTR GKVVIAGQDV TRKSTADRAG LVARVFQDPL AGSCGTLTIE ENLALAASRG KTRGLTHALN SRRRTWFRDR VASLNLGLEN RMHDRMELLS GGQRQALSLI MATLAGSEVL LLDEHTAALD PGMAEFVMEL TRTLIQETKL TTLMVTHSMR QALDYGDRTI MLHGGNILLD VTGDKRKTLG VEDLIEMFRK VRGQTLDDDA LLIE

NT Sequence

ATGATCGAAC TGTCCAATCT CGATGTCGTC TTCGGTCGCG GCACCCCGCT CGAAAAGCAG GTTCTGCATA AAATCAACCT GACCATGAAC AAGGGCTCCT TCGTCACGGT TATCGGTTCC AACGGTGCAG GCAAATCCAC AATGCTGGGC GTGCTGGCAG GCGATGTCAT ACCAACCCGT GGCAAGGTGG TCATCGCTGG CCAGGACGTG ACCCGCAAGT CTACCGCCGA TCGCGCCGGT CTGGTCGCGC GCGTGTTTCA GGACCCGCTG GCGGGAAGTT GCGGCACGCT CACCATTGAG GAAAACCTCG CTCTGGCCGC CTCGCGCGGC AAGACGCGCG GCCTCACCCA CGCGCTTAAT TCCAGGCGTC GCACCTGGTT CCGCGACCGC GTGGCAAGCC TGAACCTCGG TCTTGAAAAC CGTATGCATG ACCGCATGGA GCTTCTTTCC GGCGGCCAGC GGCAGGCGCT ATCGCTCATC ATGGCAACCC TTGCCGGTTC GGAAGTGCTG CTTTTGGATG AACATACAGC AGCACTTGAT CCCGGCATGG CCGAATTCGT GATGGAGCTG ACACGCACGT TGATCCAGGA AACCAAGCTG ACCACCTTGA TGGTAACGCA CTCCATGCGG CAGGCTCTCG ATTACGGCGA CCGCACCATC ATGCTCCATG GCGGCAATAT CCTGCTCGAT GTGACCGGCG ACAAGCGCAA GACGCTCGGT GTCGAAGATC TGATCGAGAT GTTCCGCAAG GTGCGCGGAC AGACCCTCGA TGACGATGCG CTGCTGATCG AA

Details for BrmeB.00057.b.B1.GE36858

HARVESTED ON:	5/1/2013
SEQUENCED ON:	5/8/2013
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI ELSNLDVVFG RGTPLEKQVL HKINLTMNKG SFVTVIGSNG AGKSTMLGVL AGDVIPTRGK VVIAGQDVTR KSTADRAGLV ARVFQDPLAG SCGTLTIEEN LALAASRGKT RGLTHALNSR RRTWFRDRVA SLNLGLENRM HDRMELLSGG QRQALSLIMA TLAGSEVLLL DEHTAALDPG MAEFVMELTR TLIQETKLTT LMVTHSMRQA LDYGDRTIML HGGNILLDVT GDKRKTLGVE DLIEMFRKVR GQTLDDDALL IE

Validated NT Sequence

tttnttnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatgatcga actgtccaat ctcgatgtcg tcttcggtcg cggcaccccg ctcgaaaagc aggttctgca taaaatcaac ctgaccatga acaagggctc cttcgtcacg gttatcggtt ccaacggtgc aggcaaatcc acaatgctgg gcgtgctggc aggcgatgtc ataccaaccc gtggcaaggt ggtcatcgct ggccaggacg tgacccgcaa gtctaccgcc gatcgcgccg gtctggtcgc gcgcgtgttt caggacccgc tggcgggaag ttgcggcacg ctcaccattg aggaaaacct cgctctggcc gcctcgcgcg gcaagacgcg cggcctcacc cacgcgctta attccaggcg tcgcacctgg ttccgcgacc gcgtggcaag cctgaacctc ggtcttgaaa accgtatgca tgaccgcatg gagcttcttt ccggcggcca gcggcaggcg ctatcgctca tcatggcaac ccttgccggt tcggaagtgc tgcttttgga tgaacataca gcagcacttg atcccggcat ggccgaattc gtgatggagc tgacacgcac gttgatccag gaaaccaagc tgaccacctt gatggtaacg cactccatgc ggcaggctct cgattacggc gaccgcacca tcatgctcca tggcggcaat atcctgctcg atgtgaccgg cgacaagcgc aagacgctcg gtgtcgaaga tctgatcgag atgttccgca aggtgcgcgg acagaccctc gatgacgatg cgctgctgat cgaataagtg agtannatag gatccgnnnn nnnnnnnccn nnnnnnnnnn nnnnnnnnnn nnnnnnnntn nnnnnnnnnn tngnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcg aactgtccaa tctcgatgtc gtcttcggtc gcggcacccc gctcgaaaag caggttctgc ataaaatcaa cctgaccatg aacaagggct ccttcgtcac ggttatcggt tccaacggtg caggcaaatc cacaatgctg ggcgtgctgg caggcgatgt cataccaacc cgtggcaagg tggtcatcgc tggccaggac gtgacccgca agtctaccgc cgatcgcgcc ggtctggtcg cgcgcgtgtt tcaggacccg ctggcgggaa gttgcggcac gctcaccatt gaggaaaacc tcgctctggc cgcctcgcgc ggcaagacgc gcggcctcac ccacgcgctt aattccaggc gtcgcacctg gttccgcgac cgcgtggcaa gcctgaacct cggtcttgaa aaccgtatgc atgaccgcat ggagcttctt tccggcggcc agcggcaggc gctatcgctc atcatggcaa cccttgccgg ttcggaagtg ctgcttttgg atgaacatac agcagcactt gatcccggca tggccgaatt cgtgatggag ctgacacgca cgttgatcca ggaaaccaag ctgaccacct tgatggtaac gcactccatg cggcaggctc tcgattacgg cgaccgcacc atcatgctcc atggcggcaa tatcctgctc gatgtgaccg gcgacaagcg caagacgctc ggtgtcgaag atctgatcga gatgttccgc aaggtgcgcg gacagaccct cgatgacgat gcgctgctga tcgaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat