SSGCID: Target Page

BrmeB.00057.a

Methionine ABC transporter ATP-binding protein

CENTER ID:	BrmeB.00057.a
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.00057.a.B1.GE36857	Full length( BrmeB.00057.a )		1	285

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_1076
BV-BRC:	fig\|546272.3.peg.1076
RefSeq:	YP_002732758.1
UniProt:	C0RIZ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MENGIDTQSQ PQEDQNHQTG VPMISVRDVT VSFGHQTVLD KLSLDIFQGE VLGFIGPSGS GKSVLMRTIL GLNKKQTGDI EILGRNIDKL NEMEKMAIDM RMGVLFQHGA LFSALNVLEN IQVPMREYLD LSPKLMDELA RLKVDLVGLH PDTAEKFPSE LSGGMIKRAA LARALALDPD IVFLDEPTSG LDPIGAAEFD DLIANLRDTM GLTVYMVTHD LDSLFAICDR IAVLGNKKVL VSGTIEDMLK VDDPWVKAYF RGKRARQIDT SAPSRRRPGN RLQTG

NT Sequence

ATGGAAAACG GCATCGACAC TCAGTCGCAA CCCCAGGAAG ATCAGAACCA CCAGACGGGC GTGCCGATGA TTTCGGTGCG CGACGTTACG GTTTCGTTTG GTCACCAGAC TGTTCTCGAC AAGCTTTCGC TCGATATATT CCAGGGAGAG GTCCTTGGCT TTATCGGGCC GTCCGGCTCC GGCAAGTCGG TGCTGATGCG TACCATTCTT GGCCTCAACA AGAAGCAGAC CGGCGATATC GAAATTCTCG GGCGCAATAT CGACAAGCTG AACGAGATGG AAAAGATGGC CATCGATATG CGTATGGGGG TTCTCTTTCA GCATGGCGCG CTGTTTTCCG CGCTGAACGT GCTGGAGAAC ATTCAGGTGC CGATGCGTGA ATATCTCGAC CTTTCGCCCA AACTCATGGA TGAGCTGGCG CGCCTGAAGG TCGATCTGGT GGGATTGCAC CCGGATACGG CGGAAAAATT TCCCTCCGAA CTTTCAGGCG GCATGATCAA GCGCGCCGCG CTTGCACGCG CGCTGGCGCT GGATCCGGAT ATTGTGTTTC TTGATGAGCC GACATCCGGC CTGGACCCGA TCGGTGCAGC GGAATTTGAC GATCTGATCG CAAACCTGCG CGATACGATG GGGCTGACGG TTTACATGGT GACCCATGAC CTCGACAGTC TTTTTGCAAT CTGCGACCGG ATCGCGGTTC TGGGAAACAA GAAGGTGCTC GTGAGCGGCA CCATTGAGGA CATGCTGAAG GTGGATGACC CTTGGGTGAA GGCCTATTTT CGGGGCAAGC GTGCGCGTCA GATTGATACA TCTGCGCCAA GTAGACGGCG GCCGGGCAAC CGCTTGCAGA CTGGA

Details for BrmeB.00057.a.B1.GE36857

HARVESTED ON:	5/1/2013
SEQUENCED ON:	5/8/2013
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHME NGIDTQSQPQ EDQNHQTGVP MISVRDVTVS FGHQTVLDKL SLDIFQGEVL GFIGPSGSGK SVLMRTILGL NKKQTGDIEI LGRNIDKLNE MEKMAIDMRM GVLFQHGALF SALNVLENIQ VPMREYLDLS PKLMDELARL KVDLVGLHPD TAEKFPSELS GGMIKRAALA RALALDPDIV FLDEPTSGLD PIGAAEFDDL IANLRDTMGL TVYMVTHDLD SLFAICDRIA VLGNKKVLVS GTIEDMLXVD DPWVKAYFRG KRARQIDTSA PSRXGRATAC RLXK

Validated NT Sequence

tttnttnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatggaaaa cggcatcgac actcagtcgc aaccccagga agatcagaac caccagacgg gcgtgccgat gatttcggtg cgcgacgtta cggtttcgtt tggtcaccag actgttctcg acaagctttc gctcgatata ttccagggag aggtccttgg ctttatcggg ccgtccggct ccggcaagtc ggtgctgatg cgtaccattc ttggcctcaa caagaagcag accggcgata tcgaaattct cgggcgcaat atcgacaagc tgaacgagat ggaaaagatg gccatcgata tgcgtatggg ggttctcttt cagcatggcg cgctgttttc cgcgctgaac gtgctggaga acattcaggt gccgatgcgt gaatatctcg acctttcgcc caaactcatg gatgagctgg cgcgcctgaa ggtcgatctg gtgggattgc acccggatac ggcggaaaaa tttccctccg aactttcagg cggcatgatc aagcgcgccg cgcttgcacg cgcgctggcg ctggatccgg atattgtgtt tcttgatgag ccgacatccg gcctggaccc gatcggtgca gcggaatttg acgatctgat cgcaaacctg cgcgatacga tggggctgac ggtttacatg gtgacccatg acctcgacag tctttttgca atctgcgacc ggatcgcggt tctgggaaac aagaaggtgc tcgtgagcgg caccattgag gacatgctga angtggatga cccttgggtg aaggcctatt ttcggggcaa gcgtgcgcgt cagattgata catctgcgcc aagtagagnc ggccgggcaa ccgcttgcag actggntaag tgagtaanat agnancnnnn nnnnnnnccc ccnnnnnnnn nnnnnnannn nnnnnnnnnn nnncnnncnn nnnnncnnnc ntnccnncnn nnnnnnnnnn nnnntnnnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa acggcatcga cactcagtcg caaccccagg aagatcagaa ccaccagacg ggcgtgccga tgatttcggt gcgcgacgtt acggtttcgt ttggtcacca gactgttctc gacaagcttt cgctcgatat attccaggga gaggtccttg gctttatcgg gccgtccggc tccggcaagt cggtgctgat gcgtaccatt cttggcctca acaagaagca gaccggcgat atcgaaattc tcgggcgcaa tatcgacaag ctgaacgaga tggaaaagat ggccatcgat atgcgtatgg gggttctctt tcagcatggc gcgctgtttt ccgcgctgaa cgtgctggag aacattcagg tgccgatgcg tgaatatctc gacctttcgc ccaaactcat ggatgagctg gcgcgcctga aggtcgatct ggtgggattg cacccggata cggcggaaaa atttccctcc gaactttcag gcggcatgat caagcgcgcc gcgcttgcac gcgcgctggc gctggatccg gatattgtgt ttcttgatga gccgacatcc ggcctggacc cgatcggtgc agcggaattt gacgatctga tcgcaaacct gcgcgatacg atggggctga cggtttacat ggtgacccat gacctcgaca gtctttttgc aatctgcgac cggatcgcgg ttctgggaaa caagaaggtg ctcgtgagcg gcaccattga ggacatgctg aaggtggatg acccttgggt gaaggcctat tttcggggca agcgtgcgcg tcagattgat acatctgcgc caagtagacg gcggccgggc aaccgcttgc agactggatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat