SSGCID: Target Page

BrmeB.00027.f

Butyryl-CoA dehydrogenase (EC 1.3.99.2)

CENTER ID:	BrmeB.00027.f
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.00027.f.B1.GE36856	Full length( BrmeB.00027.f )		1	380

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_3378
BV-BRC:	fig\|546272.3.peg.3378
RefSeq:	YP_002734820.1
UniProt:	C0RMN3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIRDPETLDA LVTTVRRFVR ERLVPAESRV EETNRIPDDI IEEMRAMGLF GLSIPEEYGG LGLTMEEEVR VGFELGYTSP AFRSVIGTNN GIGSQGIIAD GTDDQKKYWL PRLASGGIIG SFALTEPDVG SDAGAVRTTA ERDGDSYIIN GTKRFITNAP VAGVFTLMAR TGGSGPSGIS AFLAERDLPG LTVGTPDHKM GQRGTQTCDV YLENVRVPAT SIIGGVEGRG FKTAMKVLNR GRLHISSVCA GTAERPIEES VGFAGSRVQF GKRIAEHQMI QAMLADMSTE AYAGRCMVLD AARAFDESNV DIIRKAASCK LFCSEMVGRV ADNAVQIHGG SGYMQNYPVE HFYRDVRLFR LYEGTSQIQR IIIAREMIRE

NT Sequence

ATGATCCGCG ATCCGGAAAC ACTCGATGCG CTTGTCACAA CGGTTCGGCG TTTCGTGCGC GAACGTCTGG TACCGGCGGA AAGTCGCGTC GAAGAGACGA ACCGTATTCC TGATGACATC ATTGAAGAAA TGCGGGCCAT GGGCCTCTTT GGTTTGTCGA TCCCGGAAGA GTATGGCGGT CTTGGCCTGA CGATGGAAGA GGAAGTCCGT GTCGGTTTTG AACTTGGATA TACCTCACCA GCCTTTCGCT CCGTCATTGG TACCAATAAT GGCATCGGCT CTCAGGGCAT CATTGCCGAT GGCACAGACG ACCAGAAGAA ATATTGGCTT CCACGGCTGG CCAGCGGCGG GATAATTGGT TCATTCGCAT TGACAGAGCC TGATGTCGGT TCGGATGCCG GTGCAGTACG CACCACTGCC GAACGCGACG GAGACAGCTA TATCATCAAC GGCACGAAGC GCTTCATTAC CAATGCGCCG GTTGCCGGAG TTTTCACGCT CATGGCTCGT ACGGGTGGTT CGGGCCCATC GGGAATCAGC GCCTTTCTGG CGGAAAGGGA TTTGCCCGGT CTCACGGTCG GAACACCCGA CCACAAAATG GGCCAGAGAG GCACCCAAAC CTGCGATGTC TATCTTGAGA ATGTACGCGT TCCTGCTACA AGCATAATTG GCGGGGTCGA AGGACGCGGT TTCAAGACCG CTATGAAAGT ACTCAATCGC GGGCGTCTTC ATATATCGTC CGTCTGCGCC GGTACGGCGG AGCGTCCGAT TGAGGAATCT GTCGGCTTTG CCGGCAGCCG CGTACAGTTT GGCAAACGTA TCGCCGAACA TCAGATGATT CAGGCGATGC TGGCCGATAT GAGCACAGAA GCCTATGCGG GGCGTTGCAT GGTTCTTGAT GCCGCACGGG CCTTTGATGA AAGCAACGTC GATATCATTC GCAAAGCTGC AAGCTGCAAG TTGTTTTGTT CCGAAATGGT TGGAAGGGTT GCAGACAATG CCGTGCAGAT ACACGGCGGC TCCGGATATA TGCAGAACTA TCCGGTCGAG CACTTCTATC GCGATGTACG TTTGTTCCGC CTCTATGAGG GAACCTCGCA AATCCAGCGA ATTATCATAG CGCGCGAAAT GATAAGGGAG

Details for BrmeB.00027.f.B1.GE36856

HARVESTED ON:	5/1/2013
SEQUENCED ON:	5/8/2013
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	84

Validated AA Sequence

MRAMGLFGLS IPEEYGGLGL TMEEEVRVGF ELGYTSPAFR SVIGTNNGIG SQGIIADGTD DQKKYWLPRL ASGGIIGSFA LTEPDVGSDA GAVRTTAERD GDSYIINGTK RFITNAPVAG VFTLMARTGG SGPSGISAFL AERDLPGLTV GTPDHKMGQR GTQTCDVYLE NVRVPATSII GGVEGRGFKT AMKVLNRGRL HISSVCAGTA ERPIEESVGF AGSRVQFGKR IAEHQMIQAM LADMSTEAYA GRCMVLDAAR AFDESNVDII RKAASCKLFC SEMVGRVADN AVQIHGGSGY MQNYPVEHFY RDVRLFRLYE GTSQIQRI

Validated NT Sequence

ataattcgct ggatttgcga ggttccctca tagaggcgga acaaacgtac atcgcgatag aagtgctcga ccggatagtt ctgcatatat ccggagccgc cgtgtatctg cacggcattg tctgcaaccc ttccaaccat ttcggaacaa aacaacttgc agcttgcagc tttgcgaatg atatcgacgt tgctttcatc aaaggcccgt gcggcatcaa gaaccatgca acgccccgca taggcttctg tgctcatatc ggccagcatc gcctgaatca tctgatgttc ggcgatacgt ttgccaaact gtacgcggct gccggcaaag ccgacagatt cctcaatcgg acgctccgcc gtaccggcgc agacggacga tatatgaaga cgcccgcgat tgagtacttt catagcggtc ttgaaaccgc gtccttcgac cccgccaatt atgcttgtag caggaacgcg tacattctca agatagacat cgcaggtttg ggtgcctctc tggcccattt tgtggtcggg tgttccgacc gtgagaccgg gcaaatccct ttccgccaga aaggcgctga ttcccgatgg gcccgaacca cccgtacgag ccatgagcgt gaaaactccg gcaaccggcg cattggtaat gaagcgcttc gtgccgttga tgatatagct gtctccgtcg cgttcggcag tggtgcgtac tgcaccggca tccgaaccga catcaggctc tgtcaatgcg aatgaaccaa ttatcccgcc gctggccagc cgtggaagcc aatatttctt ctggtcgtct gtgccatcgg caatgatgcc ctgagagccg atgccattat tggtaccaat gacggagcga aaggctggtg aggtatatcc aagttcaaaa ccgacacgga cttcctcttc catcgtcagg ccaagaccgc catactcttc cgggatcgac aaaccaaaga ggcccatggc ccgcatttct tcaatgatgt catcaggaat acggttcgtc tcttcgacgc gactttccgc cggtaccaga cgttcgcgca cgaaacgccg aaccgttgtg acaagcgcat cgagtgtttc cggatc

Expected Protein Sequence

MAHHHHHHMI RDPETLDALV TTVRRFVRER LVPAESRVEE TNRIPDDIIE EMRAMGLFGL SIPEEYGGLG LTMEEEVRVG FELGYTSPAF RSVIGTNNGI GSQGIIADGT DDQKKYWLPR LASGGIIGSF ALTEPDVGSD AGAVRTTAER DGDSYIINGT KRFITNAPVA GVFTLMARTG GSGPSGISAF LAERDLPGLT VGTPDHKMGQ RGTQTCDVYL ENVRVPATSI IGGVEGRGFK TAMKVLNRGR LHISSVCAGT AERPIEESVG FAGSRVQFGK RIAEHQMIQA MLADMSTEAY AGRCMVLDAA RAFDESNVDI IRKAASCKLF CSEMVGRVAD NAVQIHGGSG YMQNYPVEHF YRDVRLFRLY EGTSQIQRII IAREMIRE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcc gcgatccgga aacactcgat gcgcttgtca caacggttcg gcgtttcgtg cgcgaacgtc tggtaccggc ggaaagtcgc gtcgaagaga cgaaccgtat tcctgatgac atcattgaag aaatgcgggc catgggcctc tttggtttgt cgatcccgga agagtatggc ggtcttggcc tgacgatgga agaggaagtc cgtgtcggtt ttgaacttgg atatacctca ccagcctttc gctccgtcat tggtaccaat aatggcatcg gctctcaggg catcattgcc gatggcacag acgaccagaa gaaatattgg cttccacggc tggccagcgg cgggataatt ggttcattcg cattgacaga gcctgatgtc ggttcggatg ccggtgcagt acgcaccact gccgaacgcg acggagacag ctatatcatc aacggcacga agcgcttcat taccaatgcg ccggttgccg gagttttcac gctcatggct cgtacgggtg gttcgggccc atcgggaatc agcgcctttc tggcggaaag ggatttgccc ggtctcacgg tcggaacacc cgaccacaaa atgggccaga gaggcaccca aacctgcgat gtctatcttg agaatgtacg cgttcctgct acaagcataa ttggcggggt cgaaggacgc ggtttcaaga ccgctatgaa agtactcaat cgcgggcgtc ttcatatatc gtccgtctgc gccggtacgg cggagcgtcc gattgaggaa tctgtcggct ttgccggcag ccgcgtacag tttggcaaac gtatcgccga acatcagatg attcaggcga tgctggccga tatgagcaca gaagcctatg cggggcgttg catggttctt gatgccgcac gggcctttga tgaaagcaac gtcgatatca ttcgcaaagc tgcaagctgc aagttgtttt gttccgaaat ggttggaagg gttgcagaca atgccgtgca gatacacggc ggctccggat atatgcagaa ctatccggtc gagcacttct atcgcgatgt acgtttgttc cgcctctatg agggaacctc gcaaatccag cgaattatca tagcgcgcga aatgataagg gagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat