BrmeB.00027.e

Butyryl-CoA dehydrogenase (EC 1.3.99.2)

CENTER ID: BrmeB.00027.e
ORGANISM: Brucella melitensis ATCC 23457
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.00027.e.B1.GE36855 Full length( BrmeB.00027.e ) 1 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeB.00027.e.B1.PS02086 Full length( BrmeB.00027.e ) 1 386

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel14466_3060
BV-BRC: fig|546272.3.peg.3060
RefSeq: WP_004686003.1
RefSeq: YP_002734538.1
UniProt: C0RLV1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHVAVDDYTD IREAVFKLCQ RFPGEYWRKI DREMAYPNEF VNALTEAGWL SILIPEEYGG AGLPLSAAAA VLEEIQKSGC NGGACHAQMY TMGTLLRHGS DEQKACYLPK IASGELRLQA FGVTEPTSGT DTGALKTTAR REGDEFIING QKIWTSRAEY SDLMLLLART SPLTDGMKKT DGLSVLIVDM AKAKNNGLTI RPIRTMMNHA TTEVFFDNLR VPVSNLIGEE GKGFRYILSG MNAERILIAA ECIGDAKWFT QKSVNYAKER SIFGRPIGQN QGIQFPIAKA YANMRAAELM VQSALALYEA GQNPGAEANM AKMLAADASF EAANACVQTH GGFGFAEEYD VERKFRETRL YQVAPISTNL ILSYIAEHVL GMPRSY
NT Sequence
ATGCATGTTG CAGTGGATGA TTATACGGAC ATTCGCGAGG CCGTTTTCAA GCTTTGCCAA AGGTTTCCGG GCGAGTACTG GCGTAAAATC GATCGTGAGA TGGCCTATCC CAACGAATTT GTGAATGCGT TGACTGAGGC AGGATGGCTC TCGATCCTTA TTCCTGAAGA GTATGGCGGA GCCGGTTTGC CGCTATCGGC GGCAGCAGCC GTACTGGAAG AAATACAAAA ATCGGGCTGC AATGGCGGTG CATGTCACGC CCAGATGTAC ACGATGGGCA CCCTCCTGAG GCACGGATCA GACGAGCAAA AAGCCTGTTA TCTGCCCAAA ATTGCATCGG GTGAGCTCCG ATTGCAAGCG TTTGGCGTGA CCGAGCCGAC CAGCGGAACG GATACGGGCG CACTTAAAAC CACAGCTCGC CGTGAGGGTG ATGAATTTAT CATCAATGGC CAGAAAATAT GGACGAGCCG CGCAGAATAC TCGGACCTCA TGTTGTTGCT GGCGCGGACC TCCCCCCTGA CGGATGGAAT GAAGAAAACC GACGGCCTTT CTGTCCTTAT CGTCGATATG GCGAAAGCAA AGAATAATGG TCTTACCATC CGCCCCATTA GAACGATGAT GAACCATGCC ACGACCGAAG TTTTCTTCGA CAATCTTCGC GTACCCGTAT CAAACTTGAT CGGCGAGGAA GGCAAAGGCT TTCGCTATAT TCTTTCGGGA ATGAACGCCG AGCGAATTCT GATCGCAGCC GAATGTATTG GCGACGCAAA ATGGTTTACG CAGAAATCAG TAAATTACGC CAAGGAGCGA TCGATATTTG GGCGTCCGAT CGGACAAAAC CAGGGTATCC AGTTTCCGAT AGCGAAAGCT TATGCAAACA TGCGGGCCGC TGAACTTATG GTGCAATCTG CTCTGGCTTT GTATGAAGCA GGCCAGAATC CGGGTGCTGA GGCGAATATG GCAAAAATGC TTGCTGCCGA TGCATCATTT GAGGCTGCAA ATGCCTGCGT TCAAACGCAT GGCGGTTTTG GATTTGCCGA AGAATATGAT GTTGAACGCA AATTCCGCGA GACCCGCCTT TATCAGGTGG CACCAATCTC GACCAACCTT ATCCTCTCAT ACATAGCCGA GCATGTTCTG GGCATGCCTC GTTCTTAT
Details for BrmeB.00027.e.B1.GE36855
HARVESTED ON: 5/1/2013
SEQUENCED ON: 5/8/2013
EXPECTED MW: 44kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 87
Validated AA Sequence
MAYPNEFVNA LTEAGWLSIL IPEEYGGAGL PLSAAAAVLE EIQKSGCNGG ACHAQMYTMG TLLRHGSDEQ KACYLPKIAS GELRLQAFGV TEPTSGTDTG ALKTTARREG DEFIINGQKI WTSRAEYSDL MLLLARTSPL TDGMKKTDGL SVLIVDMAKA KNNGLTIRPI RTMMNHATTE VFFDNLRVPV SNLIGEEGKG FRYILSGMNA ERILIAAECI GDAKWFTQKS VNYAKERSIF GRPIGQNQGI QFPIAKAYAN MRAAELMVQS ALALYEAGQN PGAEANMAKM LAADASFEAA NACVQTHGGF GFAEEYDVER KFRETRLYQV APISTNLILS YIAEHV
Validated NT Sequence
gaacatgctc ggctatgtat gagaggataa ggttggtcga gattggtgcc acctgataaa ggcgggtctc gcggaatttg cgttcaacat catattcttc ggcaaatcca aaaccgccat gcgtttgaac gcaggcattt gcagcctcaa atgatgcatc ggcagcaagc atttttgcca tattcgcctc agcacccgga ttctggcctg cttcatacaa agccagagca gattgcacca taagttcagc ggcccgcatg tttgcataag ctttcgctat cggaaactgg ataccctggt tttgtccgat cggacgccca aatatcgatc gctccttggc gtaatttact gatttctgcg taaaccattt tgcgtcgcca atacattcgg ctgcgatcag aattcgctcg gcgttcattc ccgaaagaat atagcgaaag cctttgcctt cctcgccgat caagtttgat acgggtacgc gaagattgtc gaagaaaact tcggtcgtgg catggttcat catcgttcta atggggcgga tggtaagacc attattcttt gctttcgcca tatcgacgat aaggacagaa aggccgtcgg ttttcttcat tccatccgtc aggggggagg tccgcgccag caacaacatg aggtccgagt attctgcgcg gctcgtccat attttctggc cattgatgat aaattcatca ccctcacggc gagctgtggt tttaagtgcg cccgtatccg ttccgctggt cggctcggtc acgccaaacg cttgcaatcg gagctcaccc gatgcaattt tgggcagata acaggctttt tgctcgtctg atccgtgcct caggagggtg cccatcgtgt acatctgggc gtgacatgca ccgccattgc agcccgattt ttgtatttct tccagtacgg ctgctgccgc cgatagcggc aaaccggctc cgccatactc ttcaggaata aggatcgaga gccatcctgc ctcagtcaac gcattcacaa attcgttggg ataggccatc tcacgatcga ttttacgcca gtactcgccc ggaaaccttt ggcaaagctt gaaaacggcc tcgcgaatgt ccgtataatc atccactgca a
Expected Protein Sequence
MAHHHHHHMH VAVDDYTDIR EAVFKLCQRF PGEYWRKIDR EMAYPNEFVN ALTEAGWLSI LIPEEYGGAG LPLSAAAAVL EEIQKSGCNG GACHAQMYTM GTLLRHGSDE QKACYLPKIA SGELRLQAFG VTEPTSGTDT GALKTTARRE GDEFIINGQK IWTSRAEYSD LMLLLARTSP LTDGMKKTDG LSVLIVDMAK AKNNGLTIRP IRTMMNHATT EVFFDNLRVP VSNLIGEEGK GFRYILSGMN AERILIAAEC IGDAKWFTQK SVNYAKERSI FGRPIGQNQG IQFPIAKAYA NMRAAELMVQ SALALYEAGQ NPGAEANMAK MLAADASFEA ANACVQTHGG FGFAEEYDVE RKFRETRLYQ VAPISTNLIL SYIAEHVLGM PRSY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatg ttgcagtgga tgattatacg gacattcgcg aggccgtttt caagctttgc caaaggtttc cgggcgagta ctggcgtaaa atcgatcgtg agatggccta tcccaacgaa tttgtgaatg cgttgactga ggcaggatgg ctctcgatcc ttattcctga agagtatggc ggagccggtt tgccgctatc ggcggcagca gccgtactgg aagaaataca aaaatcgggc tgcaatggcg gtgcatgtca cgcccagatg tacacgatgg gcaccctcct gaggcacgga tcagacgagc aaaaagcctg ttatctgccc aaaattgcat cgggtgagct ccgattgcaa gcgtttggcg tgaccgagcc gaccagcgga acggatacgg gcgcacttaa aaccacagct cgccgtgagg gtgatgaatt tatcatcaat ggccagaaaa tatggacgag ccgcgcagaa tactcggacc tcatgttgtt gctggcgcgg acctcccccc tgacggatgg aatgaagaaa accgacggcc tttctgtcct tatcgtcgat atggcgaaag caaagaataa tggtcttacc atccgcccca ttagaacgat gatgaaccat gccacgaccg aagttttctt cgacaatctt cgcgtacccg tatcaaactt gatcggcgag gaaggcaaag gctttcgcta tattctttcg ggaatgaacg ccgagcgaat tctgatcgca gccgaatgta ttggcgacgc aaaatggttt acgcagaaat cagtaaatta cgccaaggag cgatcgatat ttgggcgtcc gatcggacaa aaccagggta tccagtttcc gatagcgaaa gcttatgcaa acatgcgggc cgctgaactt atggtgcaat ctgctctggc tttgtatgaa gcaggccaga atccgggtgc tgaggcgaat atggcaaaaa tgcttgctgc cgatgcatca tttgaggctg caaatgcctg cgttcaaacg catggcggtt ttggatttgc cgaagaatat gatgttgaac gcaaattccg cgagacccgc ctttatcagg tggcaccaat ctcgaccaac cttatcctct catacatagc cgagcatgtt ctgggcatgc ctcgttctta ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BrmeB.00027.e.B1.PS02086
PURIFICATION DATe: 7/1/2014
CONCENTRATION: 36.1mg/ml
OBSERVED MW: 39kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 87
Protocol Notes
notes unavailable
Validated AA Sequence
MAYPNEFVNA LTEAGWLSIL IPEEYGGAGL PLSAAAAVLE EIQKSGCNGG ACHAQMYTMG TLLRHGSDEQ KACYLPKIAS GELRLQAFGV TEPTSGTDTG ALKTTARREG DEFIINGQKI WTSRAEYSDL MLLLARTSPL TDGMKKTDGL SVLIVDMAKA KNNGLTIRPI RTMMNHATTE VFFDNLRVPV SNLIGEEGKG FRYILSGMNA ERILIAAECI GDAKWFTQKS VNYAKERSIF GRPIGQNQGI QFPIAKAYAN MRAAELMVQS ALALYEAGQN PGAEANMAKM LAADASFEAA NACVQTHGGF GFAEEYDVER KFRETRLYQV APISTNLILS YIAEHV
Validated NT Sequence
gaacatgctc ggctatgtat gagaggataa ggttggtcga gattggtgcc acctgataaa ggcgggtctc gcggaatttg cgttcaacat catattcttc ggcaaatcca aaaccgccat gcgtttgaac gcaggcattt gcagcctcaa atgatgcatc ggcagcaagc atttttgcca tattcgcctc agcacccgga ttctggcctg cttcatacaa agccagagca gattgcacca taagttcagc ggcccgcatg tttgcataag ctttcgctat cggaaactgg ataccctggt tttgtccgat cggacgccca aatatcgatc gctccttggc gtaatttact gatttctgcg taaaccattt tgcgtcgcca atacattcgg ctgcgatcag aattcgctcg gcgttcattc ccgaaagaat atagcgaaag cctttgcctt cctcgccgat caagtttgat acgggtacgc gaagattgtc gaagaaaact tcggtcgtgg catggttcat catcgttcta atggggcgga tggtaagacc attattcttt gctttcgcca tatcgacgat aaggacagaa aggccgtcgg ttttcttcat tccatccgtc aggggggagg tccgcgccag caacaacatg aggtccgagt attctgcgcg gctcgtccat attttctggc cattgatgat aaattcatca ccctcacggc gagctgtggt tttaagtgcg cccgtatccg ttccgctggt cggctcggtc acgccaaacg cttgcaatcg gagctcaccc gatgcaattt tgggcagata acaggctttt tgctcgtctg atccgtgcct caggagggtg cccatcgtgt acatctgggc gtgacatgca ccgccattgc agcccgattt ttgtatttct tccagtacgg ctgctgccgc cgatagcggc aaaccggctc cgccatactc ttcaggaata aggatcgaga gccatcctgc ctcagtcaac gcattcacaa attcgttggg ataggccatc tcacgatcga ttttacgcca gtactcgccc ggaaaccttt ggcaaagctt gaaaacggcc tcgcgaatgt ccgtataatc atccactgca a
Expressed Protein Sequence
MAHHHHHHMH VAVDDYTDIR EAVFKLCQRF PGEYWRKIDR EMAYPNEFVN ALTEAGWLSI LIPEEYGGAG LPLSAAAAVL EEIQKSGCNG GACHAQMYTM GTLLRHGSDE QKACYLPKIA SGELRLQAFG VTEPTSGTDT GALKTTARRE GDEFIINGQK IWTSRAEYSD LMLLLARTSP LTDGMKKTDG LSVLIVDMAK AKNNGLTIRP IRTMMNHATT EVFFDNLRVP VSNLIGEEGK GFRYILSGMN AERILIAAEC IGDAKWFTQK SVNYAKERSI FGRPIGQNQG IQFPIAKAYA NMRAAELMVQ SALALYEAGQ NPGAEANMAK MLAADASFEA ANACVQTHGG FGFAEEYDVE RKFRETRLYQ VAPISTNLIL SYIAEHVLGM PRSY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCATG TTGCAGTGGA TGATTATACG GACATTCGCG AGGCCGTTTT CAAGCTTTGC CAAAGGTTTC CGGGCGAGTA CTGGCGTAAA ATCGATCGTG AGATGGCCTA TCCCAACGAA TTTGTGAATG CGTTGACTGA GGCAGGATGG CTCTCGATCC TTATTCCTGA AGAGTATGGC GGAGCCGGTT TGCCGCTATC GGCGGCAGCA GCCGTACTGG AAGAAATACA AAAATCGGGC TGCAATGGCG GTGCATGTCA CGCCCAGATG TACACGATGG GCACCCTCCT GAGGCACGGA TCAGACGAGC AAAAAGCCTG TTATCTGCCC AAAATTGCAT CGGGTGAGCT CCGATTGCAA GCGTTTGGCG TGACCGAGCC GACCAGCGGA ACGGATACGG GCGCACTTAA AACCACAGCT CGCCGTGAGG GTGATGAATT TATCATCAAT GGCCAGAAAA TATGGACGAG CCGCGCAGAA TACTCGGACC TCATGTTGTT GCTGGCGCGG ACCTCCCCCC TGACGGATGG AATGAAGAAA ACCGACGGCC TTTCTGTCCT TATCGTCGAT ATGGCGAAAG CAAAGAATAA TGGTCTTACC ATCCGCCCCA TTAGAACGAT GATGAACCAT GCCACGACCG AAGTTTTCTT CGACAATCTT CGCGTACCCG TATCAAACTT GATCGGCGAG GAAGGCAAAG GCTTTCGCTA TATTCTTTCG GGAATGAACG CCGAGCGAAT TCTGATCGCA GCCGAATGTA TTGGCGACGC AAAATGGTTT ACGCAGAAAT CAGTAAATTA CGCCAAGGAG CGATCGATAT TTGGGCGTCC GATCGGACAA AACCAGGGTA TCCAGTTTCC GATAGCGAAA GCTTATGCAA ACATGCGGGC CGCTGAACTT ATGGTGCAAT CTGCTCTGGC TTTGTATGAA GCAGGCCAGA ATCCGGGTGC TGAGGCGAAT ATGGCAAAAA TGCTTGCTGC CGATGCATCA TTTGAGGCTG CAAATGCCTG CGTTCAAACG CATGGCGGTT TTGGATTTGC CGAAGAATAT GATGTTGAAC GCAAATTCCG CGAGACCCGC CTTTATCAGG TGGCACCAAT CTCGACCAAC CTTATCCTCT CATACATAGC CGAGCATGTT CTGGGCATGC CTCGTTCTTA TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT