BrmeB.00010.h

2-deoxy-D-gluconate 3-dehydrogenase (EC 1.1.1.125)

CENTER ID:	BrmeB.00010.h
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.00010.h.B1.GE36850	Full length( BrmeB.00010.h )		1	227

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|546272.3.peg.3250
RefSeq:	YP_002734709.1
UniProt:	C0RMC2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGLRMVHVLA GHGARVISIS RTHAGESLCP SGGEVLEIMA DVTRPEEIAR AFDEGESRFG PISALFNNAG VAHMARALDT TRDMLEHIFE VNVAGAFFTA QEVARRMIAQ GQGGSIINVT SILGERPQKG AAAYSMSKAC VSQMTRALAL EWAAHDIRVN AISPGWFPTR INEEQLQGPA AGYLKGRNPM RRLGDPNDLD GVVLLLASDA SRYMTGSIIT VDGGHQL

NT Sequence

TTGGGCCTGC GCATGGTGCA TGTGCTGGCC GGTCATGGCG CCCGGGTGAT ATCGATTTCG CGCACCCATG CGGGCGAAAG CCTGTGCCCA TCGGGCGGGG AAGTGCTGGA AATCATGGCA GATGTAACGC GCCCGGAAGA AATTGCGCGC GCCTTCGATG AAGGCGAAAG CCGCTTCGGG CCGATCAGCG CCTTGTTCAA CAATGCCGGA GTTGCCCATA TGGCCCGCGC TCTTGATACC ACTCGCGATA TGCTGGAGCA TATTTTCGAG GTGAATGTCG CGGGTGCTTT CTTCACTGCG CAGGAGGTTG CGCGCCGGAT GATCGCACAG GGGCAGGGCG GATCGATCAT CAATGTCACG AGTATTCTGG GTGAGCGCCC GCAAAAGGGT GCGGCTGCCT ATTCGATGTC GAAAGCCTGT GTGAGCCAGA TGACGCGGGC ACTGGCGCTG GAATGGGCGG CGCATGATAT TCGCGTCAAT GCAATCTCGC CCGGATGGTT TCCAACCCGA ATCAACGAAG AGCAGTTACA GGGGCCGGCT GCGGGCTATC TCAAGGGGCG CAACCCCATG CGGCGGCTTG GTGATCCGAA CGACCTCGAC GGGGTTGTGC TGCTGCTGGC CTCGGATGCG AGCCGGTATA TGACTGGGTC GATCATTACG GTCGATGGTG GCCACCAGCT C

Details for BrmeB.00010.h.B1.GE36850

HARVESTED ON:	5/1/2013
SEQUENCED ON:	5/8/2013
EXPECTED MW:	25kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML GLRMVHVLAG HGARVISISR THAGESLCPS GGEVLEIMAD VTRPEEIARA FDEGESRFGP ISALFNNAGV AHMARALDTT RDMLEHIFEV NVAGAFFTAQ EVARRMIAQG QGGSIINVTS ILGERPQKGA AAYSMSKACV SQMTRALALE WAAHDIRVNA ISPGWFPTRI NEEQLQGPAA GYLKGRNPMR RLGDPNDLDG VVLLLASDAS RYMTGSIITV DGGHQL

Validated NT Sequence

ttnttnnntt taagaaggag atataccatg gctcaccacc accaccacca tatgttgggc ctgcgcatgg tgcatgtgct ggccggtcat ggcgcccggg tgatatcgat ttcgcgcacc catgcgggcg aaagcctgtg cccatcgggc ggggaagtgc tggaaatcat ggcagatgta acgcgcccgg aagaaattgc gcgcgccttc gatgaaggcg aaagccgctt cgggccgatc agcgccttgt tcaacaatgc cggagttgcc catatggccc gcgctcttga taccactcgc gatatgctgg agcatatttt cgaggtgaat gtcgcgggtg ctttcttcac tgcgcaggag gttgcgcgcc ggatgatcgc acaggggcag ggcggatcga tcatcaatgt cacgagtatt ctgggtgagc gcccgcaaaa gggtgcggct gcctattcga tgtcgaaagc ctgtgtgagc cagatgacgc gggcactggc gctggaatgg gcggcgcatg atattcgcgt caatgcaatc tcgcccggat ggtttccaac ccgaatcaac gaagagcagt tacaggggcc ggctgcgggc tatctcaagg ggcgcaaccc catgcggcgg cttggtgatc cgaacgacct cgacggggtt gtgctgctgc tggcctcgga tgcgagccgg tatatgactg ggtcgatcat tacggtcgat ggtggccacc agctctaagt gagtaagata ggatccggct gctaacannc ccccnngnnn nccnngannn nnnnnnnnnn tnnnanntnn nnttncngtt ncngnnnnna nnnnnangnn nnnntctnnc nnnnaangna cnnnnnnnna ttttnnnnna nnnnnnncnn ccnntc

Expected Protein Sequence

MAHHHHHHMG LRMVHVLAGH GARVISISRT HAGESLCPSG GEVLEIMADV TRPEEIARAF DEGESRFGPI SALFNNAGVA HMARALDTTR DMLEHIFEVN VAGAFFTAQE VARRMIAQGQ GGSIINVTSI LGERPQKGAA AYSMSKACVS QMTRALALEW AAHDIRVNAI SPGWFPTRIN EEQLQGPAAG YLKGRNPMRR LGDPNDLDGV VLLLASDASR YMTGSIITVD GGHQL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgggcc tgcgcatggt gcatgtgctg gccggtcatg gcgcccgggt gatatcgatt tcgcgcaccc atgcgggcga aagcctgtgc ccatcgggcg gggaagtgct ggaaatcatg gcagatgtaa cgcgcccgga agaaattgcg cgcgccttcg atgaaggcga aagccgcttc gggccgatca gcgccttgtt caacaatgcc ggagttgccc atatggcccg cgctcttgat accactcgcg atatgctgga gcatattttc gaggtgaatg tcgcgggtgc tttcttcact gcgcaggagg ttgcgcgccg gatgatcgca caggggcagg gcggatcgat catcaatgtc acgagtattc tgggtgagcg cccgcaaaag ggtgcggctg cctattcgat gtcgaaagcc tgtgtgagcc agatgacgcg ggcactggcg ctggaatggg cggcgcatga tattcgcgtc aatgcaatct cgcccggatg gtttccaacc cgaatcaacg aagagcagtt acaggggccg gctgcgggct atctcaaggg gcgcaacccc atgcggcggc ttggtgatcc gaacgacctc gacggggttg tgctgctgct ggcctcggat gcgagccggt atatgactgg gtcgatcatt acggtcgatg gtggccacca gctctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat