BrmeB.00010.g
3-hydroxyacyl-CoA dehydrogenase
| CENTER ID: | BrmeB.00010.g |
| ORGANISM: | Brucella melitensis ATCC 23457 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeB.00010.g.B1.GE36849 | Full length( BrmeB.00010.g ) | 1 | 255 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeB.00010.g.B1.PS01897 | Full length( BrmeB.00010.g ) | 1 | 255 |
Structures
4PN3
DEPOSITED: 5/22/2014
DETERMINATION: XRay
CLONE: BrmeB.00010.g.B1.GE36849
PROTEIN: BrmeB.00010.g.B1.PS01897
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|546272.3.peg.2706 |
| RefSeq: | YP_002734227.1 |
| UniProt: | C0RKZ0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQIENRVFLI TGAGSGLGAA VSKMAVEAGA KVVLLDVNAE AGEAGAKALG ASARFQRTDV ASDTDGKAAI AAAIEAFSRI DVLVNCAGVA PGEKVLGREG AHKLETFTRT ISINLIGTFN MLRLAAEAMA KNEPGQGGER GVIINTASVA AFDGQIGQAA YSASKGGVAA MTLPVARELA RHGIRVMTIA PGIFKTPMMA GMPQEVQDAL GASVPFPPRL GEPAEYAALV RHIVENQMLN GEVIRLDGAL RMAAK
NT Sequence
ATGCAGATAG AAAATCGCGT ATTTCTGATT ACCGGCGCAG GCTCCGGCCT TGGCGCTGCC GTTTCCAAAA TGGCTGTTGA GGCGGGCGCA AAGGTGGTCC TTCTCGATGT GAATGCGGAA GCAGGCGAAG CGGGCGCGAA GGCGCTTGGC GCTTCGGCCA GATTCCAGCG CACAGACGTC GCAAGCGATA CCGACGGCAA GGCTGCAATT GCAGCAGCGA TTGAAGCTTT TAGCCGCATC GACGTACTGG TCAATTGCGC CGGCGTCGCG CCGGGCGAAA AGGTGCTTGG CCGCGAAGGC GCACACAAGC TTGAAACCTT CACCCGCACC ATTTCGATCA ACCTGATCGG CACCTTCAAC ATGCTGCGCC TTGCAGCCGA AGCCATGGCG AAGAACGAAC CCGGCCAGGG CGGCGAGCGC GGTGTGATCA TCAACACGGC TTCCGTTGCC GCTTTTGACG GCCAGATCGG CCAGGCTGCC TATTCGGCCT CCAAGGGTGG CGTTGCGGCC ATGACGCTAC CCGTCGCGCG CGAACTTGCC CGCCACGGCA TTCGTGTGAT GACCATTGCG CCCGGCATTT TCAAGACCCC GATGATGGCA GGAATGCCGC AGGAAGTGCA GGACGCGCTG GGTGCTTCCG TGCCTTTCCC ACCGCGTCTT GGCGAACCGG CGGAATATGC CGCCCTCGTC CGCCATATCG TGGAAAACCA GATGTTGAAC GGCGAAGTGA TCCGTCTCGA CGGCGCACTG CGTATGGCCG CCAAG
Details for BrmeB.00010.g.B1.GE36849
| HARVESTED ON: | 5/1/2013 |
| SEQUENCED ON: | 5/8/2013 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMQ IENRVFLITG AGSGLGAAVS KMAVEAGAKV VLLDVNAEAG EAGAKALGAS ARFQRTDVAS DTDGKAAIAA AIEAFSRIDV LVNCAGVAPG EKVLGREGAH KLETFTRTIS INLIGTFNML RLAAEAMAKN EPGQGGERGV IINTASVAAF DGQIGQAAYS ASKGGVAAMT LPVARELARH GIRVMTIAPG IFKTPMMAGM PQEVQDALGA SVPFPPRLGE PAEYAALVRH IVENQMLNGE VIRLDGALRM AAK
Validated NT Sequence
atcttactca cttacttggc ggccatacgc agtgcgccgt cgagacggat cacttcgccg ttcaacatct ggttttccac gatatggcgg acgagggcgg catattccgc cggttcgcca agacgcggtg ggaaaggcac ggaagcaccc agcgcgtcct gcacttcctg cggcattcct gccatcatcg gggtcttgaa aatgccgggc gcaatggtca tcacacgaat gccgtggcgg gcaagttcgc gcgcgacggg tagcgtcatg gccgcaacgc cacccttgga ggccgaatag gcagcctggc cgatctggcc gtcaaaagcg gcaacggaag ccgtgttgat gatcacaccg cgctcgccgc cctggccggg ttcgttcttc gccatggctt cggctgcaag gcgcagcatg ttgaaggtgc cgatcaggtt gatcgaaatg gtgcgggtga aggtttcaag cttgtgtgcg ccttcgcggc caagcacctt ttcgcccggc gcgacgccgg cgcaattgac cagtacgtcg atgcggctaa aagcttcaat cgctgctgca attgcagcct tgccgtcggt atcgcttgcg acgtctgtgc gctggaatct ggccgaagcg ccaagcgcct tcgcgcccgc ttcgcctgct tccgcattca catcgagaag gaccaccttt gcgcccgcct caacagccat tttggaaacg gcagcgccaa ggccggagcc tgcgccggta atcagaaata cgcgattttc tatctgcata tggtggtggt ggtggtgagc catggtatan ctcc
Expected Protein Sequence
MAHHHHHHMQ IENRVFLITG AGSGLGAAVS KMAVEAGAKV VLLDVNAEAG EAGAKALGAS ARFQRTDVAS DTDGKAAIAA AIEAFSRIDV LVNCAGVAPG EKVLGREGAH KLETFTRTIS INLIGTFNML RLAAEAMAKN EPGQGGERGV IINTASVAAF DGQIGQAAYS ASKGGVAAMT LPVARELARH GIRVMTIAPG IFKTPMMAGM PQEVQDALGA SVPFPPRLGE PAEYAALVRH IVENQMLNGE VIRLDGALRM AAK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaga tagaaaatcg cgtatttctg attaccggcg caggctccgg ccttggcgct gccgtttcca aaatggctgt tgaggcgggc gcaaaggtgg tccttctcga tgtgaatgcg gaagcaggcg aagcgggcgc gaaggcgctt ggcgcttcgg ccagattcca gcgcacagac gtcgcaagcg ataccgacgg caaggctgca attgcagcag cgattgaagc ttttagccgc atcgacgtac tggtcaattg cgccggcgtc gcgccgggcg aaaaggtgct tggccgcgaa ggcgcacaca agcttgaaac cttcacccgc accatttcga tcaacctgat cggcaccttc aacatgctgc gccttgcagc cgaagccatg gcgaagaacg aacccggcca gggcggcgag cgcggtgtga tcatcaacac ggcttccgtt gccgcttttg acggccagat cggccaggct gcctattcgg cctccaaggg tggcgttgcg gccatgacgc tacccgtcgc gcgcgaactt gcccgccacg gcattcgtgt gatgaccatt gcgcccggca ttttcaagac cccgatgatg gcaggaatgc cgcaggaagt gcaggacgcg ctgggtgctt ccgtgccttt cccaccgcgt cttggcgaac cggcggaata tgccgccctc gtccgccata tcgtggaaaa ccagatgttg aacggcgaag tgatccgtct cgacggcgca ctgcgtatgg ccgccaagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BrmeB.00010.g.B1.PS01897
| PURIFICATION DATe: | 8/21/2013 |
| CONCENTRATION: | 31.83mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ IENRVFLITG AGSGLGAAVS KMAVEAGAKV VLLDVNAEAG EAGAKALGAS ARFQRTDVAS DTDGKAAIAA AIEAFSRIDV LVNCAGVAPG EKVLGREGAH KLETFTRTIS INLIGTFNML RLAAEAMAKN EPGQGGERGV IINTASVAAF DGQIGQAAYS ASKGGVAAMT LPVARELARH GIRVMTIAPG IFKTPMMAGM PQEVQDALGA SVPFPPRLGE PAEYAALVRH IVENQMLNGE VIRLDGALRM AAK
Validated NT Sequence
atcttactca cttacttggc ggccatacgc agtgcgccgt cgagacggat cacttcgccg ttcaacatct ggttttccac gatatggcgg acgagggcgg catattccgc cggttcgcca agacgcggtg ggaaaggcac ggaagcaccc agcgcgtcct gcacttcctg cggcattcct gccatcatcg gggtcttgaa aatgccgggc gcaatggtca tcacacgaat gccgtggcgg gcaagttcgc gcgcgacggg tagcgtcatg gccgcaacgc cacccttgga ggccgaatag gcagcctggc cgatctggcc gtcaaaagcg gcaacggaag ccgtgttgat gatcacaccg cgctcgccgc cctggccggg ttcgttcttc gccatggctt cggctgcaag gcgcagcatg ttgaaggtgc cgatcaggtt gatcgaaatg gtgcgggtga aggtttcaag cttgtgtgcg ccttcgcggc caagcacctt ttcgcccggc gcgacgccgg cgcaattgac cagtacgtcg atgcggctaa aagcttcaat cgctgctgca attgcagcct tgccgtcggt atcgcttgcg acgtctgtgc gctggaatct ggccgaagcg ccaagcgcct tcgcgcccgc ttcgcctgct tccgcattca catcgagaag gaccaccttt gcgcccgcct caacagccat tttggaaacg gcagcgccaa ggccggagcc tgcgccggta atcagaaata cgcgattttc tatctgcata tggtggtggt ggtggtgagc catggtatan ctcc
Expressed Protein Sequence
MAHHHHHHMQ IENRVFLITG AGSGLGAAVS KMAVEAGAKV VLLDVNAEAG EAGAKALGAS ARFQRTDVAS DTDGKAAIAA AIEAFSRIDV LVNCAGVAPG EKVLGREGAH KLETFTRTIS INLIGTFNML RLAAEAMAKN EPGQGGERGV IINTASVAAF DGQIGQAAYS ASKGGVAAMT LPVARELARH GIRVMTIAPG IFKTPMMAGM PQEVQDALGA SVPFPPRLGE PAEYAALVRH IVENQMLNGE VIRLDGALRM AAK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAGA TAGAAAATCG CGTATTTCTG ATTACCGGCG CAGGCTCCGG CCTTGGCGCT GCCGTTTCCA AAATGGCTGT TGAGGCGGGC GCAAAGGTGG TCCTTCTCGA TGTGAATGCG GAAGCAGGCG AAGCGGGCGC GAAGGCGCTT GGCGCTTCGG CCAGATTCCA GCGCACAGAC GTCGCAAGCG ATACCGACGG CAAGGCTGCA ATTGCAGCAG CGATTGAAGC TTTTAGCCGC ATCGACGTAC TGGTCAATTG CGCCGGCGTC GCGCCGGGCG AAAAGGTGCT TGGCCGCGAA GGCGCACACA AGCTTGAAAC CTTCACCCGC ACCATTTCGA TCAACCTGAT CGGCACCTTC AACATGCTGC GCCTTGCAGC CGAAGCCATG GCGAAGAACG AACCCGGCCA GGGCGGCGAG CGCGGTGTGA TCATCAACAC GGCTTCCGTT GCCGCTTTTG ACGGCCAGAT CGGCCAGGCT GCCTATTCGG CCTCCAAGGG TGGCGTTGCG GCCATGACGC TACCCGTCGC GCGCGAACTT GCCCGCCACG GCATTCGTGT GATGACCATT GCGCCCGGCA TTTTCAAGAC CCCGATGATG GCAGGAATGC CGCAGGAAGT GCAGGACGCG CTGGGTGCTT CCGTGCCTTT CCCACCGCGT CTTGGCGAAC CGGCGGAATA TGCCGCCCTC GTCCGCCATA TCGTGGAAAA CCAGATGTTG AACGGCGAAG TGATCCGTCT CGACGGCGCA CTGCGTATGG CCGCCAAGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT