SSGCID: Target Page

BrmeB.00010.d

3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)

CENTER ID:	BrmeB.00010.d
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.00010.d.B1.GE36846	Full length( BrmeB.00010.d )		1	254

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_2115
BV-BRC:	fig\|546272.3.peg.2115
RefSeq:	YP_002733707.1
UniProt:	C0RFT1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRQRPVALV TGGRRGIGLG IARALAAKGF DLAITDRESD EAVIHELRGL GGKVAFFKSD LAAVETHEAT VFAVLDAFGG IDCLVNNAGM GAVERGDFLA LKPENFDTIM DVNLRGTVFF TQAVVKAMLA ADEVRFPRSI VTISSVSSVM TSPERLDYCI SKAGLTAFVQ GLALRLAEAR IGVFEVRPGI IRTDMTAKVA ARYDALIEGG LVPMKRWGEA SDVGAIVAGL AGGDFIFATG SAIHADGGLS IAKL

NT Sequence

ATGAGCCGTC AAAGACCTGT AGCGCTGGTG ACTGGCGGGC GGCGGGGGAT CGGGCTTGGC ATTGCCCGCG CTCTGGCTGC CAAGGGGTTT GATCTGGCGA TCACCGACCG CGAAAGCGAC GAGGCCGTCA TCCACGAATT GCGCGGGCTT GGTGGAAAAG TGGCTTTCTT TAAAAGTGAT CTCGCTGCCG TGGAAACCCA TGAGGCGACG GTTTTTGCCG TGCTGGATGC GTTCGGCGGC ATTGATTGTC TGGTCAATAA TGCGGGTATG GGTGCGGTCG AGCGCGGCGA TTTTCTTGCC CTGAAGCCTG AAAATTTCGA CACGATCATG GATGTGAACC TGCGCGGCAC GGTGTTTTTC ACGCAGGCCG TGGTGAAGGC CATGCTGGCC GCAGACGAAG TGCGCTTTCC ACGCTCCATC GTGACGATCA GTTCGGTTTC TTCCGTGATG ACATCGCCGG AGCGGCTTGA TTACTGCATC AGCAAGGCGG GGCTGACCGC CTTCGTGCAA GGGCTGGCAC TGCGTCTGGC CGAAGCGCGG ATCGGTGTCT TCGAGGTGCG CCCCGGCATT ATCCGCACCG ATATGACGGC AAAGGTTGCC GCGCGTTATG ACGCGCTGAT CGAAGGCGGG TTGGTGCCGA TGAAGCGCTG GGGCGAGGCG AGCGATGTTG GCGCCATCGT GGCCGGGCTT GCCGGTGGCG ATTTCATCTT TGCGACCGGC TCGGCAATCC ATGCCGATGG CGGCCTGTCG ATTGCAAAAT TA

Details for BrmeB.00010.d.B1.GE36846

HARVESTED ON:	5/1/2013
SEQUENCED ON:	5/8/2013
EXPECTED MW:	28kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS RQRPVALVTG GRRGIGLGIA RALAAKGFDL AITDRESDEA VIHELRGLGG KVAFFKSDLA AVETHEATVF AVLDAFGGID CLVNNAGMGA VERGDFLALK PENFDTIMDV NLRGTVFFTQ AVVKAMLAAD EVRFPRSIVT ISSVSSVMTS PERLDYCISK AGLTAFVQGL ALRLAEARIG VFEVRPGIIR TDMTAKVAAR YDALIEGGLV PMKRWGEASD VGAIVAGLAG GDFIFATGSA IHADGGLSIA KL

Validated NT Sequence

ttantcactt ataattttgc aatcgacagg ccgccatcgg catggattgc cgagccggtc gcaaagatga aatcgccacc ggcaagcccg gccacgatgg cgccaacatc gctcgcctcg ccccagcgct tcatcggcac caacccgcct tcgatcagcg cgtcataacg cgcggcaacc tttgccgtca tatcggtgcg gataatgccg gggcgcacct cgaagacacc gatccgcgct tcggccagac gcagtgccag cccttgcacg aaggcggtca gccccgcctt gctgatgcag taatcaagcc gctccggcga tgtcatcacg gaagaaaccg aactgatcgt cacgatggag cgtggaaagc gcacttcgtc tgcggccagc atggccttca ccacggcctg cgtgaaaaac accgtgccgc gcaggttcac atccatgatc gtgtcgaaat tttcaggctt cagggcaaga aaatcgccgc gctcgaccgc acccataccc gcattattga ccagacaatc aatgccgccg aacgcatcca gcacggcaaa aaccgtcgcc tcatgggttt ccacggcagc gagatcactt ttaaagaaag ccacttttcc accaagcccg cgcaattcgt ggatgacggc ctcgtcgctt tcgcggtcgg tgatcgccag atcaaacccc ttggcagcca gagcgcgggc aatgccaagc ccgatccccc gccgcccgcc agtcaccagc gctacaggtc tttgacggct catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc gtcaaagacc tgtagcgctg gtgactggcg ggcggcgggg gatcgggctt ggcattgccc gcgctctggc tgccaagggg tttgatctgg cgatcaccga ccgcgaaagc gacgaggccg tcatccacga attgcgcggg cttggtggaa aagtggcttt ctttaaaagt gatctcgctg ccgtggaaac ccatgaggcg acggtttttg ccgtgctgga tgcgttcggc ggcattgatt gtctggtcaa taatgcgggt atgggtgcgg tcgagcgcgg cgattttctt gccctgaagc ctgaaaattt cgacacgatc atggatgtga acctgcgcgg cacggtgttt ttcacgcagg ccgtggtgaa ggccatgctg gccgcagacg aagtgcgctt tccacgctcc atcgtgacga tcagttcggt ttcttccgtg atgacatcgc cggagcggct tgattactgc atcagcaagg cggggctgac cgccttcgtg caagggctgg cactgcgtct ggccgaagcg cggatcggtg tcttcgaggt gcgccccggc attatccgca ccgatatgac ggcaaaggtt gccgcgcgtt atgacgcgct gatcgaaggc gggttggtgc cgatgaagcg ctggggcgag gcgagcgatg ttggcgccat cgtggccggg cttgccggtg gcgatttcat ctttgcgacc ggctcggcaa tccatgccga tggcggcctg tcgattgcaa aattatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat