SSGCID: Target Page

BrmeB.00010.c

7-alpha-hydroxysteroid dehydrogenase (EC 1.1.1.159)

CENTER ID:	BrmeB.00010.c
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.00010.c.B1.GE36845	Full length( BrmeB.00010.c )		1	264

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_1688
BV-BRC:	fig\|546272.3.peg.1688
RefSeq:	YP_002733321.1
UniProt:	C0REP5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAYDAFAEFR MTGHNVIITG GAQNIGAGIA KTLSGAGAKV MIADLNGDKA RQTAAAIQKE TGNECHGMKC DVTSLSDIEQ VVRETVSAFG GISTLVNNVG WGGRHEDPTA ITEEDFIAAY KLNTISAYRM SMACLPHLLK SQNATITNSG SFSSAVPAYD ILAYGTAKAA LNQMMVSLAH MLAKKVRINS VLIGTVMTEG YGSAGIDEEM QEKLRHPDNL TGRAGRPEDI ANTMLWLCSP ASGWVSGQTI NVHGGGGVTR LFGR

NT Sequence

ATGGCCTATG ATGCATTTGC CGAATTTCGC ATGACGGGCC ACAATGTCAT CATCACCGGC GGCGCACAGA ATATAGGCGC AGGCATTGCG AAAACCCTGT CAGGCGCAGG CGCAAAGGTG ATGATCGCCG ACCTCAACGG CGACAAGGCC AGGCAAACGG CGGCAGCAAT CCAGAAAGAA ACCGGCAATG AATGCCATGG CATGAAGTGC GACGTCACTT CACTTAGCGA TATCGAACAG GTGGTGCGCG AAACCGTCTC GGCTTTTGGC GGCATTTCAA CCCTCGTCAA CAATGTCGGC TGGGGCGGGC GCCATGAAGA CCCGACAGCG ATCACCGAGG AAGATTTCAT CGCAGCCTAT AAGCTGAATA CGATCAGCGC CTACCGCATG AGCATGGCTT GCCTGCCGCA TCTGCTTAAA TCGCAAAACG CGACGATCAC CAATTCCGGC TCTTTCTCCT CTGCTGTGCC AGCCTATGAC ATTCTGGCCT ATGGAACGGC GAAAGCTGCC CTCAACCAGA TGATGGTTTC GCTGGCTCAT ATGCTGGCAA AGAAGGTGCG CATCAATTCC GTGCTGATCG GCACGGTGAT GACAGAAGGT TACGGTTCAG CCGGCATCGA CGAGGAAATG CAGGAAAAGC TGCGCCATCC CGATAACCTT ACCGGGCGTG CCGGACGACC CGAAGATATC GCCAATACCA TGTTGTGGCT GTGCTCGCCC GCCAGCGGAT GGGTCAGCGG CCAGACTATC AATGTGCATG GCGGCGGCGG CGTCACGCGC CTCTTCGGCA GG

Details for BrmeB.00010.c.B1.GE36845

HARVESTED ON:	5/1/2013
SEQUENCED ON:	5/8/2013
EXPECTED MW:	29kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA YDAFAEFRMT GHNVIITGGA QNIGAGIAKT LSGAGAKVMI ADLNGDKARQ TAAAIQKETG NECHGMKCDV TSLSDIEQVV RETVSAFGGI STLVNNVGWG GRHEDPTAIT EEDFIAAYKL NTISAYRMSM ACLPHLLKSQ NATITNSGSF SSAVPAYDIL AYGTAKAALN QMMVSLAHML AKKVRINSVL IGTVMTEGYG SAGIDEEMQE KLRHPDNLTG RAGRPEDIAN TMLWLCSPAS GWVSGQTINV HGGGGVTRLF GR

Validated NT Sequence

atcttactca cttacctgcc gaagaggcgc gtgacgccgc cgccgccatg cacattgata gtctggccgc tgacccatcc gctggcgggc gagcacagcc acaacatggt attggcgata tcttcgggtc gtccggcacg cccggtaagg ttatcgggat ggcgcagctt ttcctgcatt tcctcgtcga tgccggctga accgtaacct tctgtcatca ccgtgccgat cagcacggaa ttgatgcgca ccttctttgc cagcatatga gccagcgaaa ccatcatctg gttgagggca gctttcgccg ttccataggc cagaatgtca taggctggca cagcagagga gaaagagccg gaattggtga tcgtcgcgtt ttgcgattta agcagatgcg gcaggcaagc catgctcatg cggtaggcgc tgatcgtatt cagcttatag gctgcgatga aatcttcctc ggtgatcgct gtcgggtctt catggcgccc gccccagccg acattgttga cgagggttga aatgccgcca aaagccgaga cggtttcgcg caccacctgt tcgatatcgc taagtgaagt gacgtcgcac ttcatgccat ggcattcatt gccggtttct ttctggattg ctgccgccgt ttgcctggcc ttgtcgccgt tgaggtcggc gatcatcacc tttgcgcctg cgcctgacag ggttttcgca atgcctgcgc ctatattctg tgcgccgccg gtgatgatga cattgtggcc cgtcatgcga aattcggcaa atgcatcata ggccatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcct atgatgcatt tgccgaattt cgcatgacgg gccacaatgt catcatcacc ggcggcgcac agaatatagg cgcaggcatt gcgaaaaccc tgtcaggcgc aggcgcaaag gtgatgatcg ccgacctcaa cggcgacaag gccaggcaaa cggcggcagc aatccagaaa gaaaccggca atgaatgcca tggcatgaag tgcgacgtca cttcacttag cgatatcgaa caggtggtgc gcgaaaccgt ctcggctttt ggcggcattt caaccctcgt caacaatgtc ggctggggcg ggcgccatga agacccgaca gcgatcaccg aggaagattt catcgcagcc tataagctga atacgatcag cgcctaccgc atgagcatgg cttgcctgcc gcatctgctt aaatcgcaaa acgcgacgat caccaattcc ggctctttct cctctgctgt gccagcctat gacattctgg cctatggaac ggcgaaagct gccctcaacc agatgatggt ttcgctggct catatgctgg caaagaaggt gcgcatcaat tccgtgctga tcggcacggt gatgacagaa ggttacggtt cagccggcat cgacgaggaa atgcaggaaa agctgcgcca tcccgataac cttaccgggc gtgccggacg acccgaagat atcgccaata ccatgttgtg gctgtgctcg cccgccagcg gatgggtcag cggccagact atcaatgtgc atggcggcgg cggcgtcacg cgcctcttcg gcaggtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat