SSGCID: Target Page

BrmeB.00010.b

3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)

CENTER ID:	BrmeB.00010.b
ORGANISM:	Brucella melitensis ATCC 23457
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeB.00010.b.B1.GE36844	Full length( BrmeB.00010.b )		1	219

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel14466_0767
BV-BRC:	fig\|546272.3.peg.767
RefSeq:	YP_002732470.1
UniProt:	C0RI58

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGAASARRLA QDGFAVAILS SSGKGEALAK ELGGIGVTGS NQSNDDLQKL VDQTLEKWGR IDVLVNSAGH GPRAPILEIT DEDWHKGMDT YFLNAVRPAR LVVPAMQKQK SGVIINISTA WAFEPSAMFP TSAVFRAGLA SFTKIFADTY AAENIRMNNV LPGWIDSLPT TEERRESVPM QRYGKSEEIA ATVSFLASDG AAYITGQNLR VDGGLTRSV

NT Sequence

ATGGGAGCAG CCTCGGCCCG CCGTCTGGCA CAGGACGGTT TTGCAGTTGC CATTCTGTCA TCGTCCGGCA AGGGCGAAGC CCTCGCCAAA GAATTGGGGG GCATTGGCGT TACCGGCTCG AACCAGTCCA ACGACGACCT GCAAAAGCTG GTCGACCAGA CTTTGGAAAA ATGGGGGCGG ATCGACGTTC TGGTCAACAG CGCCGGTCAT GGGCCGCGCG CACCAATTCT CGAAATTACC GACGAAGACT GGCATAAGGG AATGGACACC TATTTCCTCA ATGCCGTGCG CCCCGCGCGC CTCGTCGTGC CTGCAATGCA GAAGCAGAAA TCGGGCGTCA TCATCAATAT TTCGACCGCC TGGGCTTTCG AGCCAAGTGC GATGTTCCCG ACATCGGCTG TGTTCCGTGC AGGGCTTGCC TCCTTCACCA AAATATTTGC CGACACCTAT GCGGCTGAGA ATATCCGCAT GAACAATGTC CTCCCCGGCT GGATCGACAG CCTGCCAACA ACGGAAGAAC GCCGCGAAAG CGTGCCGATG CAGCGTTATG GGAAGAGCGA GGAAATCGCG GCAACCGTGT CGTTCCTTGC TTCCGATGGG GCGGCTTATA TCACCGGGCA GAACCTGCGT GTCGATGGCG GCCTGACACG CTCAGTC

Details for BrmeB.00010.b.B1.GE36844

HARVESTED ON:	5/1/2013
SEQUENCED ON:	5/8/2013
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG AASARRLAQD GFAVAILSSS GKGEALAKEL GGIGVTGSNQ SNDDLQKLVD QTLEKWGRID VLVNSAGHGP RAPILEITDE DWHKGMDTYF LNAVRPARLV VPAMQKQKSG VIINISTAWA FEPSAMFPTS AVFRAGLASF TKIFADTYAA ENIRMNNVLP GWIDSLPTTE ERRESVPMQR YGKSEEIAAT VSFLASDGAA YITGQNLRVD GGLTRSV

Validated NT Sequence

tttntttact ttaagaagga ganataccat ggctcaccac caccaccacc atatgggagc agcctcggcc cgccgtctgg cacaggacgg ttttgcagtt gccattctgt catcgtccgg caagggcgaa gccctcgcca aagaattggg gggcattggc gttaccggct cgaaccagtc caacgacgac ctgcaaaagc tggtcgacca gactttggaa aaatgggggc ggatcgacgt tctggtcaac agcgccggtc atgggccgcg cgcaccaatt ctcgaaatta ccgacgaaga ctggcataag ggaatggaca cctatttcct caatgccgtg cgccccgcgc gcctcgtcgt gcctgcaatg cagaagcaga aatcgggcgt catcatcaat atttcgaccg cctgggcttt cgagccaagt gcgatgttcc cgacatcggc tgtgttccgt gcagggcttg cctccttcac caaaatattt gccgacacct atgcggctga gaatatccgc atgaacaatg tcctccccgg ctggatcgac agcctgccaa caacggaaga acgccgcgaa agcgtgccga tgcagcgtta tgggaagagc gaggaaatcg cggcaaccgt gtcgttcctt gcttccgatg gggcggctta tatcaccggg cagaacctgc gtgtcgatgg cggcctgaca cgctcagtct aagtgagtaa gataggatcc ggctgnnnna nncccnnnnn nnnnnnnnan nnnnnnnnnn nnnnnnntnn nncnnnnnnn cnnnnnnggn nntggnnncn ccnnnnnnng aaccnnncnn gnnnnnnncc nnngnnnnnn ngnnnnnnnn cnccnannnn nnannnnngn tatncnntnc ccnannnnnn ncttannnnn nnngnnnntn gntnnantcn ngnnnnnnnn nncngnnntn nangancnnn nnnnntcnnn nnccnnnnna nnngtnnnnn nnnnnannnn nnnnnnnnta nnnncnnnnc tgnnnnnnnn nnnnnncnnn ttnnntnngn nnnnnnnnnn nnnnannnna atnnnnnng

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggag cagcctcggc ccgccgtctg gcacaggacg gttttgcagt tgccattctg tcatcgtccg gcaagggcga agccctcgcc aaagaattgg ggggcattgg cgttaccggc tcgaaccagt ccaacgacga cctgcaaaag ctggtcgacc agactttgga aaaatggggg cggatcgacg ttctggtcaa cagcgccggt catgggccgc gcgcaccaat tctcgaaatt accgacgaag actggcataa gggaatggac acctatttcc tcaatgccgt gcgccccgcg cgcctcgtcg tgcctgcaat gcagaagcag aaatcgggcg tcatcatcaa tatttcgacc gcctgggctt tcgagccaag tgcgatgttc ccgacatcgg ctgtgttccg tgcagggctt gcctccttca ccaaaatatt tgccgacacc tatgcggctg agaatatccg catgaacaat gtcctccccg gctggatcga cagcctgcca acaacggaag aacgccgcga aagcgtgccg atgcagcgtt atgggaagag cgaggaaatc gcggcaaccg tgtcgttcct tgcttccgat ggggcggctt atatcaccgg gcagaacctg cgtgtcgatg gcggcctgac acgctcagtc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat